Tag: plant tissue culture

The capacity to generate a whole new plant from any cell is called totipotency. That whole plant could be regenerated from explants ( any part of plant that taken out and grown under sterile conditions in a special nutritive media). 

Micropropagation is the tissue culture technique used for rapid vegetative multiplication of ornamental plants and fruit trees by using small sized explants.

Diving to depth can result in mechanical distortion and tissue compression, especially in gas-filled spaces of the body. Absorption of nitrogen into blood can cause high blood nitrogen levels which can exert amniotic effect. Lungs of the deep-diver cannot serve as a source of oxygen during the dive. 

An explant is a part of the plant that taken out and grown in special nutritive media under sterile conditions. This is the process to generate a whole plant in tissue culture.

Micropropagation is culture of apical shoots, axillary buds and meristems on suitable nutrient medium to produce a genetically identical progeny. Macropropagation is production of progeny by means of conventional techniques including cuttings, air-layering and grafting. Production of genetically identical cell lineage or organisms is called as cloning. It includes both conventional and tissues culture techniques. Asexual propagation refers to production of progeny by single parent plant, it can occur through vegetative propagules (rhizome, tubers and bulb) or by artificial methods (cutting, and grafting).

Plant callus is a mass of unorganized cells derived from plant tissue for use in biological research and biotechnology. In plant biology, callus cells are those cells that cover a plant wound. Callus formation is induced from plant tissues after surface sterilization and plating onto in vitro tissue culture medium. Plant growth regulators, such as auxins, cytokinins and gibberellins are supplemented into the medium to initiate callus formation or somatic embryogenesis.

Explant is any portion taken from a plant that will be used to initiate a culture. It can be a portion of the shoot or of the leaves or even just some cells. It is a piece of sterile plant tissue to be used for tissue culture under aseptic conditions. Inoculant is the active material used in an inoculation. Clones are organisms that are exact genetic copies. Somacloning is cloning of plant with a single cell.