Genetically engineered insulin - class-XII
Description: genetically engineered insulin | |
Number of Questions: 39 | |
Created by: Blackmamba | |
Tags: cell biology and biotechnology biology genetic technology applied biology zoology applications of biotechnology |
Humulin is _________.
Insulin has 51 amino acids arranged in
After removal of signal peptide, the secretory protein involved in formation of insulin is known as
Assertion: Humilin synthesizes in the form of proinsulin.
Reason: C - peptide is not seen in mature insulin.
Genetically engineered human insulin is called as
Diabetes is caused due to the deficiency of insulin in the blood.
Scientists have been able to splice the eukaryotic gene for the production of human insulin into the genome of bacterial cells and induce those bacteria to express the gene and produce insulin. This suggests which of the following?
Human insulin can be mass produced using genetically modified bacteria. The human insulin gene is added to a bacteria strain, and the bacteria will produce the human protein. This process demonstrates that
Humulin is
What is used in production of insulin by genetic engineering?
Multiple Choice Question
Globular protein of ~ 6kDaconsisting of 51 amino acids arranged in two polypeptide chains held by disulphide bridge is
Genetic engineering has helped in production of
Which one of correct?
Maturation of proinsulin into insulin takes place after
Recombinant DNA bearing ampicillin resistance gene is passed in E. coli. The latter are spread on agar plates containing ampicillin. Then
Escherichia coli having plasmids for production of human insulin is cultured in medium containing __________.
Some of the steps involved in the production of humulin are given below. Choose the correct sequence
(i) Synthesis of insulin gene artificially
(ii) Culturing recombinant E. coli in bioreactors
(iii) Purification of humulin
(iv) Insertion of human insulin gene into a plasmid
(v) Introduction of recombinant plasmid into E.coli
(vi) Extraction of a recombinant gene product from E. coli
In the technique of recombinant insulin production, the genes for $\alpha$- and $\beta$ - polypeptides were inserted into the plasmid by the side of __________.
Human insulin is being commercially produced from transgenic species of _____________.
Identify the wrong statement with reference to the structure of human insulin :
What is the advantage of clinical use of humulin over use of conventional ox or pig insulin __________________.
Find out the wrong statement :
Which of the following companies started selling $Humulin$ in the year 1983?
Which of the following statements regarding the structure of proinsulin and mature insulin are not correct?
(i) Proinsulin is made up of three polypeptide chains A, B, and C.
(ii) C-polypeptide chain with 33 amino acids is removed prior to insulin formation.
(iii) Mature insulin is made up of 51 amino acids and arranged in two polypeptide chains-A and B.
(iv) Polypeptide chain A has 30 amino acids and polypeptide chain B has 21 amino acids.
(v) Polypeptide chains A and B are interconnected by only one S-S linkage.
Insulin is a/an.
Human insulin is being commercially produced from a transgenic species of
__________ is a globular protein of $\sim 6\, kDa$ consisting of $51$ amino acids, arranged in $2$ polypeptide chains held together by disulphide bridge.
Pick up correct matching set of scientists in field of biotechnology.
List I | List II | ||
---|---|---|---|
A | Cohen | 1 | Structure of insulin |
B | Sharpey-Schafer | 2 | Biofuel cells |
C | F. Sanger | 3 | Established bacterial gene banks |
D | V. B. D. Skermman | 4 | Totipotency in plants |
5 | Coined insulin |
One important achievement of genetic engineering has been the production of
The first hormone artificially produced by culturing bacteria is
Genetically engineered bacteria have been used in the commercial production of
Genetically engineered bacteria have been used in commercial production of
Cyanogen bromide is employed in ____________.
During the processing of proinsulin into mature insulin
Some of the steps involved in the production of humulin are given. Choose the correct sequence (i) Synthesis of gene (DNA) for human insulin artificially (ii) Culturing recombinant E.coli in bioreactors (iii) Purification of humulin (iv) Insertion of human insulin gene into plasmid (v) Introduction of recombinant plasmid into E. coli (vi) Extraction of recombinant gene product from E. coli.
Which of the following statements is not correct?
Select the correct options to fill up the blanks.
(i) ___enzymes is crucial for the immune system to function and its absence is caused by the deletion of a gene.
(ii) Insulin consists of _and _that are linked together by _.
(iii) Transgenic mice are being used to test the safety of the __.
(iv)___involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
The first step in production of insulin using E.coli is