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Biotechnology (GATE)

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CD 8 is a transmembrane glycoprotein present in

  1. cytotoxic T cells
  2. helper T cells
  3. suppressor T cells
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: C
Explanation:

CD 8 is a transmembrane glycoprotein present in suppressor T cells.

Which of the following is a pair of non-protein amino acids?

  1. Ornithine and Citrulline

  2. Glycine and Tyrosine

  3. Valine and Arginine

  4. Ornithine and Tyrosine

  5. Arginine and Citrulline


Correct Option: A
Explanation:

This option is correct because Ornithine and Citrulline are non-protein amino acids.

Which of the following sets represents synthetic estrogens?

  1. Chlorotrianisene, Estrone, Estriol

  2. Diethylstilbestrol, Estrone, Fosfestrol

  3. Estriol, Diethylstilbestrol, Fosfestrol

  4. Chlorotrianisene, Diethylstilbestrol, Fosfestrol

  5. Estrone, Estriol, Fosfestrol


Correct Option: D
Explanation:

Correct answer. Chlorotrianisene is a non-steroidal synthetic estrogen that was formerly used for the treatment of menopause, deficiencies in ovary function, and prostate cancer. Diethylstilbestrol is a synthetic non-steroidal estrogen that was first synthesised in 1938. Fosfestrol is a synthetic estrogen used as an antineoplastic agent.

Match the items given in column - I with those given in column - II and select the correct option.

 
Column – I
Column – II
P. Xanthoproteic reaction 1. Identification of tryptophan
Q. Edman degradation 2. Identification of tyrosine
R. Millon’s test 3. Determination of the amino acid sequence of a protein
S. Hopkins-Cole reaction 4. Detection of aromatic amino acids
  1. P – 1, Q – 2, R – 3, S – 4

  2. P – 1, Q – 2, R – 4, S – 3

  3. P – 2, Q – 1, R – 4, S – 3

  4. P – 4, Q – 3, R – 2, S – 1

  5. P – 1, Q – 3, R – 2, S – 4


Correct Option: D
Explanation:

This option is correct because all are correctly matched. Aromatic amino acids can be detected by xanthoproteic reaction. Edman degradation can be used to determine the amino acid sequence of a protein. Millon’s test is used for identification of tyrosine. Hopkins-Cole reaction is used for identification of tryptophan.

The ability of the component cells of callus to form a whole plant is known as

  1. redifferentiation

  2. dedifferentiation

  3. organ culture

  4. micropropagation

  5. grafting


Correct Option: A
Explanation:

The ability of the component cells of callus to form a whole plant is known as redifferentiation.

Which of the following statements is/are correct about microbiosensors?

  1. Microbiosensors are based on piezoelectric effect.
  2. These can be integrated on one chip and are useful for measuring various substrates in a small amount of sample solution simultaneously.
  3. It is possible to develop disposable transducers for biosensors through mass production.
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: E
Explanation:

Correct answer. Microbiosensors can be integrated on one chip and are useful for measuring various substrates in a small amount of sample solution simultaneously. It is possible to develop disposable transducers for biosensors through mass production.

Which of the following sets represents cytokinins?

  1. IAA, IBA, NAA

  2. Kinetin, Zeatin, BAP

  3. Kinetin, IAA, IBA

  4. Kinetin, Zeatin, IBA

  5. Zeatin, NAA, IBA


Correct Option: B
Explanation:

Correct answer. Kinetin is a type of cytokinin, a class of plant hormone that promotes cell division. Zeatin is a plant hormone derived from the purine adenine. It is a member of the plant growth hormone family known as cytokinins. 6-Benzylaminopurine (BAP) is a first-generation synthetic cytokinin that elicits plant growth and development responses, setting blossoms and stimulating fruit richness by stimulating cell division.

Which of the following is a technique for introduction of foreign gene into a cell?

  1. Amniocentesis

  2. Transfection

  3. Pharming

  4. Cloning

  5. Embryo transfer


Correct Option: B
Explanation:

Transfection refers to the introduction of foreign gene into a cell. Transfection (the delivery of DNA or RNA into eukaryotic cells) is a powerful tool used to study and control gene expression. Cloned genes can be transfected into cells for biochemical characterisation, mutational analyses, investigation of the effects of gene expression on cell growth, investigation of gene regulatory elements, and to produce a specific protein.

Match the items in column - I with those in column - II and select the correct option.

 
Column – I
Column – II
A. Cycloheximide 1. Streptomyces griseolus
B. Anisomycin 2. Streptomyces griseus
C. Bialaphos 3. Streptomyces hygroscopicus
  1. A - 1, B - 2, C - 3

  2. A - 1, B - 3, C - 2

  3. A - 2, B - 1, C - 3

  4. A - 2, B - 3, C - 1

  5. A - 3, B - 2, C - 1


Correct Option: C
Explanation:

Correct answer. Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Anisomycin, also known as flagecidin, is an antibiotic produced by Streptomyces griseolus which inhibits protein synthesis. Bialaphos is a natural herbicide produced by the bacteria Streptomyces hygroscopicus and Streptomyces viridochromeogenes.

Assertion: The normal role of restriction endonucleases in bacterial cells is to degrade the bacterial chromosome into small pieces during replication. Reason: Bacterial cells protect their own DNA from degradation by restriction endonucleases by deleting all recognition sites from the genome.

Directions: In the following question, there are two statements, Assertion (A) and Reason (R). Consider both the statements independently and mark your answer as under:

1. A and R both are correct and R is the correct explanation of A.
2. A and R both are correct and R is not the correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.

  1. A and R both are correct and R is the correct explanation of A.

  2. A and R both are correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R both are incorrect.


Correct Option: E
Explanation:

Correct answer. The normal role of restriction endonucleases in bacterial cells is to degrade invading phage DNA. Bacterial cells protect their own DNA from degradation by restriction endonucleases by methylating the DNA at the sites that the enzyme recognises.

Match the items in column - I with those in column - II and select the correct option.

 
Column – I
Column – II
A. Replication of the genome 1. C phase
B. Formation of cleavage furrow in animal cells 2. S phase
C. Primary growth phase of a cell 3. M phase
D. Spindle formation 4. G1 phase
  1. A – 1, B – 2, C – 3, D – 4

  2. A – 2, B – 1, C – 4, D – 3

  3. A – 3, B – 2, C – 1, D – 4

  4. A – 2, B – 3, C – 4, D – 1

  5. A – 4, B – 3, C – 2, D – 1


Correct Option: B
Explanation:

Correct answer. In the cell cycle, replication of the genome occurs in the S phase. During C phase, a cleavage furrow is formed in animal cells. G1 is the primary growth phase of a cell. The spindle forms in the M phase.

Match the items in column - I with those in column - II and select the correct option.

 
Column – I
Column – II
A. Gibberellins 1. Stimulate embryo or shoot development
B. Cytokinins 2. Promote cell division
C. Auxins 3. Causes plant cells to grow
  1. A - 1, B - 2, C - 3

  2. A - 1, B - 3, C - 2

  3. A - 2, B - 1, C - 3

  4. A - 2, B - 3, C - 1

  5. A - 3, B - 2, C - 1


Correct Option: A
Explanation:

Correct answer is: Gibberellins are used to stimulate embryo or shoot development. Cytokinins are growth regulators which promote cell division. Auxins are growth regulators that cause plant cells to grow.

Random amplified polymorphic DNA is a technique for

  1. the cross linkage of antigens by antibodies

  2. revealing intra-specific variation and diversity between species

  3. selection of somaclones that are resistant to herbicides

  4. reduction or elimination of the expression of individual genes

  5. transferring geometric shapes on a mask to the surface of a silicon wafer


Correct Option: B
Explanation:

Random amplified polymorphic DNA (RAPD) is a method that reveals intra-specific variation and diversity between species.

Match the items in column - I with those in column - II and select the correct option.

 
Column – I
Column – II
P. Hybridoma 1. for monoclonal antibody production
Q. Cyanogen bromide 2. for breaking bond between insulin and –galactosidase
R. Ti plasmid 3. for introducing foreign genes into plants
S. PCR 4. for gene amplification
  1. P - 1, Q - 2, R - 3, S - 4

  2. P - 2, Q - 1, R - 4, S - 3

  3. P - 3, Q - 2, R - 1, S - 4

  4. P - 4, Q - 3, R - 2, S - 1

  5. P - 1, Q - 2, R - 4, S - 3


Correct Option: A
Explanation:

Correct answer. Hybridoma is a cell that is produced in the laboratory from the fusion of an antibody-producing lymphocyte and a non-antibody-producing cancer cell, usually a myeloma or lymphoma. It proliferates and produces a continuous supply of a specific monoclonal antibody. During recombinant insulin synthesis, the bond between insulin polypeptide and galactosidase can be removed by using cyanogen bromide. The Ti plasmid (tumor-inducing plasmid) of agrobacterium tumefaciens has been developed as a vehicle for introducing foreign genes into plants. Polymerase Chain Reaction (PCR), is a technology used for amplifying DNA sequences.

Assertion: The inhibition of glycolysis by oxygen is referred to as Pasteur effect. Reason: This is due to inhibition of the enzyme phosphofructokinase by ATP and citrate.

Directions: In the following question, there are two statements, Assertion (A) and Reason (R). Consider both the statements independently and mark your answer as under:

1. A and R both are correct and R is the correct explanation of A.
2. A and R both are correct and R is not the correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.

  1. A and R both are correct and R is the correct explanation of A.

  2. A and R both are correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R both are incorrect.


Correct Option: A
Explanation:

The inhibition of glycolysis by oxygen is referred to as Pasteur effect because this is due to inhibition of the enzyme phosphofructokinase by ATP and citrate (formed in the presence of O2).

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