0

Life Sciences (UGC / NET)

Description: CSIR UGC NET Lifesciences Life Sciences
Number of Questions: 15
Created by:
Tags: CSIR UGC NET Lifesciences Life Sciences
Attempted 0/15 Correct 0 Score 0

Exome analysis is useful in

  1. complete analysis of genes of an organism

  2. sequencing of intervening sequences

  3. getting information about protein coding sequences

  4. All of the above

  5. Only 2 and 3


Correct Option: C
Explanation:

Exons are protein coding sequences of gene. In exome analysis, exons are sequenced.

Which of the following statements is/are true about isoelectric point of a protein?

  1. All proteins with isoelectric point below pH 7.0 will have negative charge and move towards positive electrode.

  2. All proteins with isoelectric point above pH 7.0 will have positive charge and move towards negative electrode.

  3. At isoelectric point, the net charge of protein depends on the nature of aminoacid present in the protein.

  4. All of the above

  5. Only 1 and 2


Correct Option: E
Explanation:

Options 1 and 2 are true, but 3 is false.

Which of the following statements is/are true about the generation of pH gradient in isoelectric focusing of protein?

  1. pH gradient is generated using ampholytes.

  2. Ampholytes have high buffering capacity at isoelectric point.

  3. Ampholytes are mixture of heteropolymers of oligoamino and oligo carboxylic acids.

  4. Only 1 and 2

  5. All of the above


Correct Option: E
Explanation:

All the statements are true.

Which of the following is/are thermostable DNA polymerase?

  1. Taq

  2. Vent

  3. Pfu

  4. None of these

  5. All of the above


Correct Option: E
Explanation:

All of the above are thermostable DNA polymerase.

Which of the following statements is/are about the intracellular ion concentration (Na, K, Ca) as compared to the concentration of that particular ion in the blood?

  1. Concentration of Na is higher inside the cell.

  2. Concentration of K is lower inside the cell.

  3. Concentration of Ca is higher inside the cell.

  4. Concentration of potassium is high inside the cell.

  5. Options 2 and 3 are correct.


Correct Option: D
Explanation:

In vertebrates, cytosolic concentration of potasium ion is 20 to 40 times higher than blood.

The first stable compound of C4 cycle is

  1. oxaloacetic acid

  2. phospho enol pyruvate

  3. ribulose 1,5-diphosphate(RuDP)

  4. fructose 1,6 diphosphate

  5. phosphoglyceric acid


Correct Option: A
Explanation:

In C4 plants, phospho enol pyruvate combines with carbon dioxide to form first stable compound oxaloacetic acid.

In biotechnology, dioxygenin is used for

  1. detection of fluid mosaic model of biological membranes

  2. detection of protein in the sample

  3. detecting antibodies

  4. detection of nucleic acids

  5. None of these


Correct Option: D
Explanation:

Dioxygenin is useful for the labelling and detection of nucleic acids.

Which of the following statements is true about nissil bodies?

  1. It takes part in DNA replication.

  2. It is made up of rough endoplasmic reticulum (RER).

  3. Nissil bodies are involved in transcription.

  4. Nissil bodies are involved in proof reading while DNA replication.

  5. Nissil bodies are made up of golgi bodies.


Correct Option: B
Explanation:

Nissil bodies are made up of rough endoplasmic reticulum.

Which of the following statements is true about enolpyruvylshikimate phosphate (EPSP) synthase?

  1. It converts phosphoenol pyruvate to pyruvate.

  2. Glyphosate induces EPSP synthase.

  3. Inhibition of EPSP synthase blocks aromatic acid synthesis.

  4. Underproduction of EPSP synthase is helpful in somatic embryogenesis.

  5. EPSP synthase is not required for plant cells.


Correct Option: C
Explanation:

Glyphosate inhibits EPSP synthase, thereby inhibiting aromatic acid biosynthasis.

Alkaptonuria is a condition in which

  1. oxidation of homogentisic acid takes place in the kidney

  2. urine becomes black while exposed to air

  3. patients lack enzymes to oxidise homogentisic acid

  4. Both 1 and 2 are correct.

  5. Both 2 and 3 are correct.


Correct Option: E
Explanation:

Both options 2 and 3 are correct.

Which of the following statements is true about polytene chromosome?

  1. They are inert cellular objects seen in drosophilla.

  2. Banding pattern of polytene chromosome is constant in individuals of one species.

  3. Puffs seen in polytene chromosomes are the sites of gene expression.

  4. Giant chromosomes are not useful in studies of gene expression system.

  5. Formation of giant chromosomes happen in adult fly.


Correct Option: C
Explanation:

Puffs seen in polytene chromosomes are the sites of gene expression, where DNA strands are unwinded and actively take part in transcription.

Which of the following statements is correct about transcription factors?

  1. Transcription factors help in unwinding DNA.

  2. Transcription factors help in recognising promoter sequence.

  3. Transcription factors recognise ori site.

  4. Transcription factors are involved in proof reading of newly synthesised RNA.

  5. None of these


Correct Option: B
Explanation:

Transcription factors help in recognising promoter sequence in template DNA where RNA polymerase binds.

In a bacterial gene, the sequence TTGACA is known as

  1. pribnow box

  2. consensus sequence

  3. conservative sequence

  4. ori site

  5. None of these


Correct Option: B
Explanation:

TTGACA is a conserved sequence seen in 35 base pair upstream of initiation site of transcription. This is known as consensus sequence.

In development of a plant embryo, perisperm is

  1. integument

  2. primary endosperm nucellus

  3. part of nucellus persisting as a thin, peppery covering

  4. micropyle

  5. seed stalk


Correct Option: C
Explanation:

During development of a plant embryo, small portion of nucellus persists as a peppery covering called perisperm.

Which of the following sequences is true for prokaryotic transcription?

  1. Separation of DNA strands, joining of RNA polymerase, release of sigma factor, longation complex formation

  2. Separation of DNA strands, release of sigma factor, joining of RNA polymerase, elongation complex formation

  3. Joining of RNA polymerase, separation of DNA strands, release of sigma factor, longation complex formation

  4. Separation of DNA strands, joining of RNA polymerase, elongation complex formation, release of sigma factor

  5. Separation of DNA strands, elongation complex formation, joining of RNA polymerase, release of sigma factor


Correct Option: A
Explanation:

In prokaryotes, first DNA strands separate, then RNA polymerase adds to template DNA, then the sigma factor releases and elongation complex forms.

- Hide questions