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Genetic Engineering and Gene Therapy

Description: Genetic Engineering and Gene Therapy Quiz
Number of Questions: 15
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Tags: genetic engineering gene therapy biotechnology
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What is the process of modifying an organism's DNA to change its traits called?

  1. Genetic Engineering

  2. Gene Therapy

  3. Recombinant DNA Technology

  4. Transgenesis


Correct Option: A
Explanation:

Genetic engineering involves altering the genetic material of an organism to introduce new traits or modify existing ones.

Which technique is commonly used to insert foreign DNA into a host organism?

  1. Polymerase Chain Reaction (PCR)

  2. Gel Electrophoresis

  3. Restriction Enzyme Digestion

  4. Transformation


Correct Option: D
Explanation:

Transformation is a process by which foreign DNA is introduced into a host organism, typically through the use of a vector such as a plasmid or virus.

What is the term for the process of transferring a gene from one organism to another?

  1. Gene Cloning

  2. Gene Therapy

  3. Transgenesis

  4. Genetic Modification


Correct Option: C
Explanation:

Transgenesis involves the transfer of a gene from one organism to another, resulting in the recipient organism expressing the transferred gene.

Which enzyme is commonly used to cut DNA at specific sequences?

  1. DNA Ligase

  2. DNA Polymerase

  3. Restriction Enzyme

  4. Helicase


Correct Option: C
Explanation:

Restriction enzymes are used to cut DNA at specific sequences, generating fragments that can be manipulated in genetic engineering techniques.

What is the term for the process of joining DNA fragments together?

  1. DNA Ligation

  2. DNA Replication

  3. DNA Transcription

  4. DNA Translation


Correct Option: A
Explanation:

DNA ligation is the process of joining DNA fragments together using an enzyme called DNA ligase, creating a continuous DNA molecule.

Which technology allows for the amplification of specific DNA sequences?

  1. Polymerase Chain Reaction (PCR)

  2. Gel Electrophoresis

  3. Restriction Enzyme Digestion

  4. Transformation


Correct Option: A
Explanation:

Polymerase Chain Reaction (PCR) is a technique used to amplify specific DNA sequences by repeatedly copying the target region.

What is the term for the process of separating DNA fragments based on their size?

  1. Polymerase Chain Reaction (PCR)

  2. Gel Electrophoresis

  3. Restriction Enzyme Digestion

  4. Transformation


Correct Option: B
Explanation:

Gel electrophoresis is a technique used to separate DNA fragments based on their size, allowing for the analysis of DNA samples.

Which type of gene therapy involves replacing a defective gene with a functional copy?

  1. Gene Augmentation

  2. Gene Silencing

  3. Gene Editing

  4. Gene Replacement


Correct Option: D
Explanation:

Gene replacement therapy involves replacing a defective gene with a functional copy to restore normal gene function.

What is the term for the process of introducing genetic material into cells using a viral vector?

  1. Transduction

  2. Transformation

  3. Transfection

  4. Gene Editing


Correct Option: A
Explanation:

Transduction is the process of introducing genetic material into cells using a viral vector, allowing for the delivery of therapeutic genes.

Which technology allows for the precise editing of DNA sequences?

  1. Polymerase Chain Reaction (PCR)

  2. Gel Electrophoresis

  3. Restriction Enzyme Digestion

  4. CRISPR-Cas9


Correct Option: D
Explanation:

CRISPR-Cas9 is a gene editing technology that allows for the precise modification of DNA sequences, enabling targeted changes to the genome.

What is the term for the process of introducing genetic material into cells using a non-viral vector?

  1. Transduction

  2. Transformation

  3. Transfection

  4. Gene Editing


Correct Option: C
Explanation:

Transfection is the process of introducing genetic material into cells using a non-viral vector, such as a plasmid or liposome.

Which type of gene therapy involves introducing a new gene into cells to compensate for a defective gene?

  1. Gene Augmentation

  2. Gene Silencing

  3. Gene Editing

  4. Gene Replacement


Correct Option: A
Explanation:

Gene augmentation therapy involves introducing a new gene into cells to compensate for a defective gene, providing additional copies of the functional gene.

What is the term for the process of inactivating a defective gene to prevent its expression?

  1. Gene Augmentation

  2. Gene Silencing

  3. Gene Editing

  4. Gene Replacement


Correct Option: B
Explanation:

Gene silencing involves inactivating a defective gene to prevent its expression, thereby reducing the production of harmful proteins.

Which type of gene therapy involves modifying the expression of a gene without altering its sequence?

  1. Gene Augmentation

  2. Gene Silencing

  3. Gene Editing

  4. Gene Replacement


Correct Option: C
Explanation:

Gene editing involves modifying the expression of a gene without altering its sequence, allowing for precise changes to gene regulation.

What is the term for the process of introducing genetic material into cells using a physical method, such as electroporation or microinjection?

  1. Transduction

  2. Transformation

  3. Transfection

  4. Gene Editing


Correct Option: B
Explanation:

Transformation is the process of introducing genetic material into cells using a physical method, such as electroporation or microinjection, bypassing the need for a vector.

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