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DNA Replication (Medical Entrance)

Description: proteins
Number of Questions: 15
Created by:
Tags: Polymerase DNA replication Okazaki fragments DNA Replication Replication of DNA Machinery and Enzymes involved in DNA Replication
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Which polymerase is the first to participate in DNA synthesis?

  1. DNA pol I

  2. DNA pol II

  3. DNA pol III

  4. DNA pol IV


Correct Option: C
Explanation:

DNA pol III is the first polymerase to participate in DNA replication. 

Which of the following enzymes fills the necessary nucleotides between the okazaki fragments?

  1. DNA pol III

  2. DNA pol II

  3. DNA pol I

  4. DNA ligase


Correct Option: C
Explanation:

DNA pol I fills the necessary nucleotides at gaps formed by excision of RNA primer between the okazaki fragments.

Which function is performed by 3'-5' exonuclease active site of DNA pol I?

  1. Polymerisation

  2. Proofreading

  3. Joining of okazaki fragments

  4. Excision of RNA primer


Correct Option: B
Explanation:

The proofreading function of DNA polymerase I is carried out by a 3' to 5' exonuclease. 

The synthesis of DNA takes place in

  1. 5' to 3' direction

  2. 3' to 5' direction

  3. no specific direction

  4. 5' to 5' direction


Correct Option: A
Explanation:

DNA chain growth occurs in 5' to 3' direction. 

Who discovered enzyme DNA polymerase I?

  1. T Kornberg and Malcolm

  2. Anderson et. Al

  3. Arthur Kornberg

  4. Temin and Baltimore


Correct Option: C
Explanation:

Arthur Kornberg is credited for the discovery of DNA pol I in 1956.

Which of the following represents the correct order of enzymes participating in DNA replication?

  1. Primase - Helicase - ssBP - Polymerase

  2. Primase - ssBP - Helicase - Polymerase

  3. Helicase - ssBP - Primase - Polymerase

  4. Helicase - Primase - ssBP - Polymerase


Correct Option: C
Explanation:

DNA replication starts when helicase unzips DNA helix.............ssBP stabilises the unwound strands..............primase synthesises RNA primer...........and then, polymerase carries out the synthesis.

The origin sequences of DNA replication are rich in

  1. AG

  2. GC

  3. AT

  4. TC


Correct Option: C
Explanation:

The AT base pairs have only two hydrogen bonds, which are easy to unzip. Therefore, AT-rich sequences act as origin of DNA replication.

Which enzyme catalyses the elongation of the leading strand?

  1. RNA polymerase

  2. Helicase

  3. DNA polymerase

  4. Ligase


Correct Option: C
Explanation:

DNA polymerase catalyses the elongation of the leading strand.

RCR endonucleases create a nick to create a free hydroxyl in the DNA of

  1. retroviruses

  2. adenoviruses

  3. ssDNA viruses

  4. cellular life forms


Correct Option: C
Explanation:

 The 5′ end of the nicked strand is transferred to a tyrosine residue on the nuclease and the free 3′ OH group is used by the DNA polymerase to synthesise the new strand.

Which enzyme moves ahead of DNA helicase during DNA replication?

  1. DNA polymerase

  2. DNA gyrase

  3. Primase

  4. DNA ligase


Correct Option: B
Explanation:

DNA gyrase moves ahead of DNA helicase, removing the torques which come in the way.

What is the function of enzyme primase?

  1. Adding DNA primer to enable replication of DNA

  2. Adding RNA primer to enable replication of DNA

  3. Priming DNA for replication by preventing formation of nicks

  4. Unwinding double helix to prime it for DNA replication


Correct Option: B
Explanation:

Primase adds RNA primer to enable replication of DNA.

Reverse transcriptase is a special type of

  1. RNA polymerase

  2. DNA polymerase

  3. primase

  4. DNA gyrase


Correct Option: B
Explanation:

It polymerises DNA from a template of RNA.

Primasome is a complex of primase with

  1. DNA gyrase

  2. DNA helicase

  3. DNA polymerase

  4. reverse transcriptase


Correct Option: B
Explanation:

Primasome is a complex of primase with DNA helicase.

What is replicated in semi-conservative process?

  1. mRNA only

  2. DNA only

  3. mRNA and tRNA only

  4. mRNA, tRNA and DNA only


Correct Option: B
Explanation:

Semi-conservative model replicates DNA only as DNA has two strands which can act template for synthesis of new DNA.

Which enzyme regulates the overwinding or underwinding of DNA molecule during DNA replication?

  1. DNA gyrase

  2. DNA ligase

  3. Primase

  4. Topoisomerase


Correct Option: D
Explanation:

During DNA replication, topoisomerase regulates the overwinding or underwinding of DNA.

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