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Biotechnology and Its Applications - 1

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Match the biologists given in Column I with their specific fields of achievements given in Column II.

 
Column I Column II
A. Alec Jefferey i. Cytogenetics and radiation genetics in plants
B. M. S. Swaminathan ii. Function of DNA ligase
C. Eli Lily iii. DNA finger printing technology
D. Mertz and Devis iv. Preparation of humulin
  1. A – ii, B – i, C – iv, D – iii

  2. A – iii, B – i, C – iv, D – ii

  3. A – iv, B – iii, C – ii, D – i

  4. A – ii, B – iv, C – i, D – iii


Correct Option: B
Explanation:

M. S. Swaminathan studied cytogenetics and radiation genetics in plants.

OCT, XAM and CAM are

  1. plasmids used in DNA probe

  2. plasmids helpful in bioremediation

  3. plasmids used to produce disease-resistant plants

  4. plasmids to promote conjugation

  5. none of the above


Correct Option: B
Explanation:

OCT, XAM and CAM are plasmids seen in Pseudomonas putida as anti-pollutants.

The process of preserving germ plasm in frozen state is called

  1. cryopreservation

  2. cold storage

  3. in situ preservation

  4. vernalisation


Correct Option: A
Explanation:

 Cryopreservation or cryoconservation is a process where cells, whole tissues, or any other substances susceptible to damage caused by chemical reactivity or time are preserved by cooling to sub-zero temperatures.

Colchicine brings about:

  1. chromosome aberrations

  2. doubling of chromosomes

  3. gene mutations

  4. quick replication


Correct Option: B
Explanation:

  Colchicine is a toxic chemical that is often used to induce polyploidy in plants. Basically, the colchicine prevents the microtubule formation during cell division, thus the chromosomes do not pull apart like they normally do. The end result is a cell that now has double the number of chromosomes that it would normally have.

A chorion is

  1. a bag like structure within a pregnant woman's uterus containing the amniotic fluid that surrounds a fetus

  2. a membrane that originates around an embryo and contributes to the formation of the placenta

  3. a bag like structure in the uterus that is connected to a fetus by the umbilical cord

  4. another term used for umblical cord


Correct Option: B
Explanation:

The chorion develops around the embryo early in a pregnancy. Later, as a fetus develops from the embryo, the chorion fuses with the amnion.

The practice of growing of two or more crops simultaneously on the same piece of land at the same time is called

  1. Inter-cropping

  2. Mixed cropping

  3. Multiple cropping

  4. Crop rotation


Correct Option: A
Explanation:

Intercropping is a multiple cropping practice involving growing two or more crops in proximity. The most common goal of intercropping is to produce a greater yield on a given piece of land by making use of resources that would otherwise not be utilized by a single crop.

Hypervariables are useful in

  1. DNA fingerprinting

  2. genetic engineering

  3. cloning

  4. gene shearing


Correct Option: C
Explanation:

Hypervariables are useful in cloning.A hypervariable region (HVR) is a location within nuclear DNA or the D-loop of mitochondrial DNA in which base pairs of nucleotides repeat (in the case of nuclear DNA) or have substitutions (in the case of mitochondrial DNA).

Monosomy and Trisomy are represented as:

  1. 2n + 1, 2n + 3

  2. 2n - 1, 2n - 2

  3. 2n, 2n + 1

  4. 2n -1, 2n + 1


Correct Option: D
Explanation:

 The term "monosomy" is used to describe the absence of one member of a pair of chromosomes.The term "trisomy" is used to describe the presence of an extra chromosome — or three instead of the usual pair.

Yeast-two hybrid system is used to study

  1. yeast replication

  2. yeast protein synthesis

  3. protein-protein interaction

  4. transcription


Correct Option: C
Explanation:

The "yeast two-hybrid system," uses transcriptional reporters in yeast to indirectly reflect the interaction between two proteins. The term "two-hybrid" derives from the two classes of chimeric, or "hybrid," proteins used in each screen.

Directions: The following question has four choices out of which ONLY ONE is correct.

What is antisense technology?

  1. RNA polymerase producing DNA

  2. A cell displaying a foreign antigen used for synthesis of antigens

  3. Production of somaclonal variants in tissue cultures

  4. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene


Correct Option: D
Explanation:

 When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene is antisense technology.

Industrial production of enzymes, vitamins, vaccines, biofertilisers, etc. is done by

  1. tissue culture

  2. cloning

  3. fusion techniques

  4. biotechnology


Correct Option: D
Explanation:

Biotechnology helps in industrial production of enzymes, vitamins, vaccines, biofertilisers, etc.

The first human hormone produced by recombinant DNA technology was

  1. STH

  2. thyroxin

  3. insulin

  4. None of these


Correct Option: C
Explanation:

The first human hormone produced by recombinant DNA technology was insulin.

A transgene can

  1. prevent the expression of a native gene

  2. modify an existing biosynthetic pathway

  3. produce a protein that itself produces a phenotype of interest

  4. All of the above


Correct Option: D
Explanation:

 The correct answer is option 4.

Electroporation in genetics is

  1. introduction of genetic material to a transgenic animal

  2. introduction of genetic material through viruses

  3. manipulation of cells by exposing them to strong electrical field

  4. injecting DNA into the egg through electrophoresis


Correct Option: C
Explanation:

Electroporation is a technique used to add certain substances, such as a piece of DNA, into the host cell.

Shotgun technique is employed in

  1. stitching of genes

  2. isolation of genes

  3. sequencing of DNA

  4. insertion of genomic clones into a vector


Correct Option: D
Explanation:

Shotgun technique is employed in insertion of genomic clones into a vector. 

Useful genes are incorporated into a variety through genetic engineering method of crop improvement by means of

  1. plasmids

  2. microinjection

  3. co-cultivation

  4. All of the above


Correct Option: D
Explanation:

Useful genes are incorporated into a variety through genetic engineering method of crop improvement by means of plasmids, co-cultivation and microinjection.

A radioisotope used to label proteins differently from nucleic acids is

  1. 14C

  2. 15N

  3. 32P

  4. 35S


Correct Option: D
Explanation:

A radioisotope used to label proteins differently from nucleic acids is 35S. 

Modern detergents contain enzyme preparations of

  1. thermophiles

  2. acidophiles

  3. alkaliphiles

  4. thermoacidophiles


Correct Option: C
Explanation:

A recent development in detergent enzymes has been the introduction of an alkaline-stable fungal cellulase preparation for use in washing cotton fabrics. 

PCR based markers have been developed to

  1. create terminate codon

  2. manufacture antibiotics

  3. differentiate different biotypes of gall midge

  4. obtain soil microorganisms with the help of specific enzyme reductase


Correct Option: C
Explanation:

Polymerase chain reaction (PCR) based markers have been developed to differentiate different biotypes of gall midge.

Mendel is famous for his experiments on:

  1. Pisum sativum

  2. Drosophila melanogaster

  3. Neurospora crassa

  4. Oenothera lamarkiana


Correct Option: A
Explanation:

  Mendel is known for pea-plant experiments and subsequent theories on genetics. During a seven year period, Mendel experimented with pea plants in the garden owned in his monastery.

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