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Conservation of ecosystem - class-XII

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A collection of cloned DNA fragments that represents the entire genome is called genomic library.

  1. True

  2. False


Correct Option: A
Explanation:

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA

So, the correct option is 'True'.

Valuable plant materials likely to become irretrievably lost in the wild or cultivation are kept preserved in viable conditions in

  1. Gene library

  2. Gene pools

  3. Gene banks

  4. Bio-reserves


Correct Option: C
Explanation:

Gene banks are a type of biorepository, which preserve genetic material. For plants, this could be by freezing cuttings from the plant, or stocking the seeds (e.g., in a seed bank). For animals, this is the freezing of sperm and eggs in zoological freezers until further need. With corals, fragments are taken which are stored in water tanks under controlled conditions.
Plant genetic material in a 'gene bank' is preserved at $-196^0$C in liquid nitrogen as mature seed (dry).

'Biosphere reserve' is an example of

  1. In vivo conservation

  2. Ex situ conservation

  3. In situ conservation

  4. All of the above


Correct Option: C
Explanation:

A biosphere reserve is an ecosystem with plants and animals of unusual scientific and natural interest. It is a title given by UNESCO to help protect the sites. The plan is to promote management, research and education in ecosystem conservation. This includes the 'sustainable use of natural resources'. 
In situ conservation is on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species.

The loss of the sum total and variety of all the genes and their alleles present in a population or species is called as

  1. Genetic conservation

  2. Genetic erosion

  3. Species extinction

  4. Gene pooling


Correct Option: B
Explanation:

Genetic erosion is a process whereby an already limited gene pool of an endangered species of plant or animal diminishes even more, when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. 

The term is sometimes used in a narrow sense, such as when describing the loss of particular alleles or genes, as well as being used more broadly, as when referring to the loss of varieties or even whole species.
Genetic erosion occurs because each individual organism has many unique genes, which get lost when it dies without getting a chance to breed. 

Germplasm is

  1. Obsolete and improved genetic material

  2. Genetic material of related wild type

  3. Both a and b

  4. Improved genetic material


Correct Option: C
Explanation:

Germplasm is a term used to describe living genetic resources, such as seeds or tissue, maintained for the purpose of breeding, preservation, and other research uses. These resources may take the form of seed collections stored in seed banks, trees growing in nurseries, animal breeding lines maintained in animal breeding programs or gene banks, etc. Germplasm collections can range from collections of wild species to elite, domesticated breeding lines that have undergone extensive human selection.

Greatest genetic diversity is found in areas where 

  1. Plants grow wild

  2. Crops are cultivated

  3. Species originated

  4. Production is the highest


Correct Option: C
Explanation:

Genetic diversity refers to the variety of genes within a species. Each species is made up of individuals that have their own particular genetic composition. Within a species there may also be discrete populations with distinctive genes. Speciation is the evolutionary process by which new biological species arise. The biologist Orator F. Cook was the first to coin the term 'speciation' for the splitting of lineages or "cladogenesis," as opposed to "anagenesis" or "phyletic evolution" occurring within lineages.
More diversity will be introduced to gene pool when speciation occurs; as a result of which more and more species will be produced.

The species used as natural genetic engineer is

  1. Agrobacterium tumefaciens

  2. Bacillus thuringiensis

  3. Aspergillus

  4. Drosophila


Correct Option: A
Explanation:

Agrobacterium tumefaciens will transfer genes (T-DNA region of Ti plasmid) into the plant that produces its food or nutrient called opines (opines are not needed for plants). Apart from that, it also integrates genes for cell division and proliferation leading to crown gall disease, so that more number of cells result in more production of opines. Because of this reason it is called as a 'natural genetic engineer'.

The three top most major crops of the world in terms of major staple food are?

  1. Maize > Rice >Wheat

  2. Rice > Wheat > Maize

  3. Wheat > Maize > Rice

  4. Rice > Maize >Wheat


Correct Option: A
Explanation:

A staple food, sometimes simply referred to as a staple, is a food that is eaten routinely, and in such quantities that it constitutes a dominant portion of a standard diet in a given population, supplying a large fraction of the needs for energy-rich materials and generally a significant proportion of the intake of other nutrients as well. Most people live on a diet based on just a small number of staples.
Staple foods vary from place to place, but typically they are inexpensive or readily available foods that supply one or more of the three organic macronutrients needed for survival and health: carbohydrates, proteins, and fats.
Wheat (Triticum spp.) is a cereal grain, originally from the levant region of the Near East but now cultivated worldwide. In 2013, world production of wheat was 713 million tons, making it the third most-produced cereal after maize (1,016 million tons) and rice (745 million tons). 

Which one will be searched for obtaining maximum genetic diversity?

  1. Recent varieties

  2. Old varieties

  3. Wild relatives

  4. All of the above


Correct Option: D
Explanation:

Genetic diversity refers to the total number of genetic characteristics in the genetic makeup of the species. Genetic diversity serves as a way for populations to adapt to changing environments.
In order to capture the maximum genetic diversity of a species, recent, old, wild - all kind of varieties will be considered and taken into account and searched.
Thus, the correct answer is option (D),  'All of the above'.

The genome of an extinct species can be preserved in

  1. Gene Bank

  2. National park

  3. Herbarium

  4. None of the above


Correct Option: D
Explanation:

Extinction of a particular animal or plant species occurs when there are no more individuals of that species alive anywhere in the world - the species has died out. When it does not exist at all , preserving it also not possible.

Genetic erosion is due to

  1. Deforestation

  2. Shifting cultivation

  3. Adoption of genetically uniform varieties

  4. All of the above


Correct Option: D
Explanation:

Genetic erosion is a process whereby an already limited gene pool of an endangered species of plant or animal diminishes even more when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. Low genetic diversity in a population of wild animals and plants leads to a further diminishing gene pool, inbreeding and a weakening immune system and fast tracks that species towards eventual extinction. 

Wild varieties of plants must be conserved to

  1. Destroy ecosystem

  2. Feeding wild animals

  3. Future evolution

  4. Incorporate useful traits in future crop varieties


Correct Option: D
Explanation:

Wild varieties of plants must be conserved because they are of utmost value to plant breeders for producing improved and favourable varieties of plants by selecting useful genes from a wide range of their wild relatives. In other words, new and improved varieties of crops are derived from their wild relatives by genetic modification. Useful traits are like high yielding, disease resistant, stress resistant etc.

Germplasm is present in

  1. Somatic cells

  2. Growing points

  3. Reproductive cells

  4. Both (a) and (c)


Correct Option: C
Explanation:

Germplasm is a living tissue from which new plants can be grown. It can be a seed or another plant part like a leaf, a piece of stem, pollen or even just a few cells that can be turned into a whole plant. Germplasm contains the information for a species genetic makeup, a valuable natural resource of plant diversity. 

Cryopreservation is carried out at

  1. -10 to -20$^{0}$C

  2. -50 to -60$^{0}$C

  3. -196$^{0}$C

  4. -100$^{0}$C


Correct Option: C
Explanation:
Cryopreservation is the preservation of cells and tissue by freezing.
Cryopreservation is based on the ability of certain small molecules to enter cells and prevent dehydration and formation of intracellular ice crystals, which can cause cell death and destruction of cell organelles during the freezing process. 
Most systems of cellular cryopreservation use a controlled-rate freezer. This freezing system delivers liquid nitrogen into a closed chamber into which the cell suspension is placed. Careful monitoring of the rate of freezing helps to prevent rapid cellular dehydration and ice-crystal formation. In general, the cells are taken from room temperature to approximately $−190^0$C in a controlled-rate freezer. The frozen cell suspension is then transferred into a liquid-nitrogen freezer maintained at extremely cold temperatures with nitrogen in either the vapour or the liquid phase. Cryopreservation based on freeze-drying does not require use of liquid-nitrogen freezers.

Virus-free clones are obtained from

  1. Callus

  2. Embryoids

  3. Haploid culture

  4. Shoot tip culture


Correct Option: D
Explanation:

Shoot tip culture is the culture of terminal part of shoot to a plant in in-vitro condition or in lab called as shoot tip culture. Mostly the shoot tip culture used for obtain disease free plant without genetically changes. The plant which affected by any contaminant mostly by viruses have saved by shoot tip culture, because shoot tip of any affected plant is less or free from contamination (viruses, bacteria, fungus). 

Other cause of use to shoot tip culture is plant shoot tip are more efficient to cultivation or differentiation in-vitro, because cells of them newly generated and healthy comparison to other parts.

Dwarf variety of wheat was developed by

  1. M.S. Swaminathan

  2. Vavilov

  3. Borlaug

  4. Both A and C


Correct Option: C
Explanation:

Norman Borlaug developed a special breed of dwarf wheat that resisted a wide spectrum of plant pests and diseases and produced two to three times more grain than the traditional varieties. During the period from 1949-55, M S Swaminathan undertook research on potato, wheat, rice and jute genetics, after which he worked on Mexican dwarf wheat varieties.

The concept of "Centres of Origin of Cultivated Plants" was put forward by

  1. Swaminathan

  2. Mendel

  3. Vavilov

  4. Borlaug


Correct Option: C
Explanation:

Vavilov worked on global genetic diversity of cultivated plants throughout his life, from which he developed several major theories that have played an important role in the development of botany, genetics and plant breeding. One such theory among several is "Theory of centres of origin of cultivated plants" (1920).

Genomic DNA library is

  1. Packing of donor DNA in a collection of vectors.

  2. A collection of gene vectors.

  3. Collection of organisms for extracting DNA.

  4. A collection of literature about DNA.


Correct Option: A
Explanation:

A genomic library is a collection of total genomic DNA from a single organism. This DNA is packed in a population of identical vectors for storage. Each vector contains a different insert of DNA. 


So, the correct option is 'Packing of donor DNA in a collection of vectors.'

Gene library consists of

  1. All fragments of a genome

  2. Frozen germplasm

  3. Tissue culture

  4. Living bacteria having clones of genes


Correct Option: D
Explanation:

A gene library can be defined as a collection of living bacteria colonies that have been transformed with different pieces of DNA from the organism. These DNA fragments are the sources of the genes of interest. 

So, the correct option is 'Living bacteria having clones of genes'.

Genetic erosion is

  1. Deforestation

  2. Shifting cultivation

  3. Adopting genetically uniform varieties

  4. All of the above


Correct Option: D
Explanation:

Genetic erosion is a process, whereby an already limited gene pool of an endangered species of plant or animal diminishes even more when individuals from the surviving population die off without getting a chance to meet and breed with others in their endangered low population. The term is sometimes used in a narrow sense, such as when describing the loss of particular alleles or genes, as well as being used more broadly, as when referring to the loss of varieties or even whole species.
Genetic erosion occurs because each individual organism has many unique genes which get lost when it dies without getting a chance to breed. Low genetic diversity in a population of wild animals and plants leads to a further diminishing gene pool inbreeding and a weakening immune system can then "fast track" that species towards eventual extinction.

Vavilov collected............. varieties of Wheat

  1. 26,000

  2. 2,600

  3. 4,600

  4. 260


Correct Option: A
Explanation:

Vavilov collected not less than 26,000 varieties of wheat alone, were kept in cultivation at Leningrad. These collections formed the basis for new theories on the origin of cultivated plants and laid the foundation for the future improvement of crop plants and of one of the largest and oldest seed banks in the world today.

Which one will be best for germplasm collection?

  1. Britain

  2. France

  3. Portugal

  4. Peru


Correct Option: B
Explanation:

Germplasm is a term used to describe living genetic resources such as seeds or tissue, maintained for the purpose of breeding, preservation, and other research uses. These resources may take the form of seed collections stored in seed banks, trees growing in nurseries, animal breeding lines maintained in animal breeding programs or gene banks, etc. Germplasm collections can range from collections of wild species to elite, domesticated breeding lines that have undergone extensive human selection.
Holstein-Friesian (HF) gene bank collections were established in France  to conserve genetic diversity for this breed. The cryobanks have captured substantial amounts of genetic diversity for the HF compared with the current populations. A substantial part of the US, French, and Dutch collections seems to be genetically similar. On the other hand, the US collection in particular represents an interesting reservoir of HF genes of the past. Gene banks can play an important role in conserving genetic diversity within livestock breeds over time, and may support industry in the future when needed.

Dwarfing gene of wheat is

  1. Pal - 1

  2. Atomita - 1

  3. Norin - 10

  4. Pelita - 2


Correct Option: C
Explanation:

The Rht - B1b and Rht - D1b are dwarfing genes, derived from Norin - 10. Norin - 10, which was a semi - dwarf variety bred in Japan and released in 1935. Norin - 10 was used in order to improve lodging resistance in winter wheat. 

Which is used to select genes from genomic library?

  1. Cloning vectors

  2. DNA probes

  3. Gene targets

  4. Restriction enzymes


Correct Option: B
Explanation:

DNA probes are stretches of single-stranded DNA used to detect the presence of complementary nucleotide sequences (target sequences) by hybridization. Genomic library comprises large number of genes in the form of different nucleotide sequences of DNA fragments and they can be selected with the help of DNA probes.

So, the correct option 'DNA probes'.

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