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Microbiology

Description: Microbiology Microscopes SEM TEM Microscopy Methods in Microbiology
Number of Questions: 15
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Tags: Microbiology Microscopes SEM TEM Microscopy Methods in Microbiology
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Who coined the term 'microscope'?

  1. Galileo Galilei

  2. Zacharias Janssen

  3. Robert Hooke

  4. Giovanni Faber

  5. Antonie van Leeuwenhoek


Correct Option: D
Explanation:

Giovanni Faber (1625) coined the term 'microscope'.

The lens found in the eyepiece of a light microsope is called

  1. diaphragm

  2. ocular

  3. nosepiece

  4. coarse

  5. condenser


Correct Option: B
Explanation:

The lens found in the eyepiece of a light microsope is called ocular.

Microscopes are parfocal. What does it mean?

  1. The image will remain approximately in focus if the magnification changes.

  2. The image will move from focus if the magnification changes.

  3. Coarse knob is used to adjust the image.

  4. Diaphragm is used to adjust the focus.

  5. The image is seen with a dark background.


Correct Option: A
Explanation:

Correct answer

 

The Nobel Prize was awarded to Eric Betzig, William Moerner and Stefan Hell for development in which of the following fields?

  1. Scanning electron microscopy

  2. Scanning tunnelling microscopy

  3. Fluorescence microscopy

  4. Nomarski microscopy

  5. Ballistic electron emission microscopy


Correct Option: C
Explanation:

The most recent development in light microscope is fluorescence microscopy. On October 8, 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig, William Moerner and Stefan Hell for the development of super-resolved fluorescence microscopy.

Which part of a light microscope is used to regulate the amount of passing light?

  1. Condenser

  2. Diaphragm

  3. Fine focus

  4. Coarse

  5. Mechanical stage


Correct Option: B
Explanation:

An adjustable diaphragm located beneath the stage is used to regulate the amount of light that passes through.

Which of the following microscopes uses gold coated aluminum stubs for specimen mounting?

  1. Scanning electron microscope

  2. Confocal microscope

  3. Stereoscope microscope

  4. Transmission electron microscope

  5. Compound microscope


Correct Option: A
Explanation:

Specimen is mounted on aluminum stubs and is coated with gold in SEM.

Which of the following options is an optical technique that increases the sensitivity of standard optical microscope to directly visualise nanometric films?

  1. CMOS

  2. SARFAS

  3. CCD

  4. Quartz

  5. SNOM


Correct Option: B
Explanation:

SARFAS  is an optical technique that increases the sensitivity of standard optical microscope to a point at which it becomes possible to directly visualise nanometric films.

What should be the respective magnifications for lens settings with objectives of 4x, 10x, 45x and 97x on a microscope with 15x oculars?

  1. 3.75x, 1.5x, 0.33x and 0.15x

  2. 0.26x, 0.66x, 3x and 6.46x

  3. 55x, 145x, 670x and 1450x

  4. 60x, 150x, 675x and 1455x

  5. 30x, 75x, 337.5x and 727.5x


Correct Option: D
Explanation:

Magnification = objective x ocular Therefore, For 4x objective, magnification = 4 x 15 = 60x  For 10x objective, magnification = 150x For 45x objective, magnification = 675x For 97x objective, magnification = 1455x

Which of the following microscopes uses vaccum medium for microscopy?

  1. Light microscope

  2. Confocal microscope

  3. Stereoscope microscope

  4. Transmission electron microscope

  5. Compound microscope


Correct Option: D
Explanation:

In TEM, samples are placed in vacuum.

Which of the following microscopes manages focusing by digital computer motorised focusing mechanism?

  1. Compound microscope

  2. Stereoscope microscope

  3. Confocal microscope

  4. Scanning electron microscope

  5. Transmission electron microscope


Correct Option: C
Explanation:

Focusing in confocal microscope is managed by digital computer motorised focusing mechanism.

Which of the following microscopes produce(s) 3D images?

  1. Stereoscope microscope

  2. Confocal microscope

  3. Scanning electron microscope

  4. Transmission electron microscope

  5. Both (1) and (3)


Correct Option: E
Explanation:

Both produce 3D images.

Match the following:

 
(a) Ernst Ruska (i) Scanning tunnelling microscope
(b) Max Knoll (ii) Atomic force microscope
(c) Gerd Binnig and Heinrich Rohrer (iii) Transmission electron microscope
(d) Gerd Binnig, Quate and Gerber (iv) Scanning electron microscope
  1. (a) – (i), (b) – (ii), (c) – (iii), (d) – (iv)

  2. (a) – (iii), (b) – (ii), (c) – (i), (d) – (iv)

  3. (a) – (iii), (b) – (iv), (c) – (i), (d) – (ii)

  4. (a) – (i), (b) – (iii), (c) – (ii), (d) – (iv)

  5. (a) – (iv), (b) – (iii), (c) – (ii), (d) – (i)


Correct Option: C
Explanation:

Correct match: (a) – (iii), (b) – (iv), (c) – (i), (d) – (ii)

Why is immersion oil put between the slide and the lens in a compound microscope?

  1. To prevent decrease in resolution

  2. To prevent increase in resolution

  3. To enhance decreasing resolution

  4. To minimise the resolving power of condenser

  5. None of these


Correct Option: A
Explanation:

Decrease in resolution can be prevented by putting immersion oil between the slide and the lens.

Which of the following is an advantage of in-vivo staining?

  1. It is used for colouring of cells or structures that have been removed from their biological context.

  2. It reveals the locations where certain chemical reactions are taking place.

  3. Techniques like fixation can be used as diagnostic tools.

  4. Techniques like sampling can be used repeatedly.

  5. It visualises fixed cells.


Correct Option: B
Explanation:

In-vivo staining's usual purpose is to reveal cytological details that might otherwise not be apparent.

Which of the following microscopes uses laser light for providing specimen contrast?

  1. Stereoscope microscope

  2. Confocal microscope

  3. Scanning electron microscope

  4. Transmission electron microscope

  5. Compound microscope


Correct Option: B
Explanation:

Laser light with dichromatic mirror concentrated at the pinhole is used in confocal microscope.

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