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Biology Test

Description: GeneticsGenetics and EvolutionBiotechnology and its ApplicationBiotechnology and Its Applications
Number of Questions: 25
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Tags: Genetics Genetics and Evolution Biotechnology and its Application Biotechnology and Its Applications
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The vector for transfer of DNA to plants is

  1. T1 plasmid

  2. pBR322

  3. YAC

  4. M13

Show answer
Correct Option: A
Explanation:

Correct. T1 plasmid is used as cloning vector,it is used to deliver to genes of interest into plants. IT is produced by Agrobacterium Tumifaciens.

Golden rice is rich in

  1. vitamin A

  2. vitamin B

  3. vitamin C

  4. vitamin D

Show answer
Correct Option: A
Explanation:

It is a variety of Oryza sativa  produced by genetic engineering to synthesize beta carotene, a precursor of vitamin A. This option is correct.

The father of genetic engineering is

  1. Cohen and Boyer (1973)

  2. Arber, Nathan and Smith(1970)

  3. Paul Berg (1972)

  4. Boliver and Rodriguez

Show answer
Correct Option: C
Explanation:

He is father of genetic engineering.

Restriction enzymes are known as

  1. Molecular blades

  2. Molecular scissors

  3. Molecular cutters

  4. Vectors

Show answer
Correct Option: B
Explanation:

Correct.Restriction enzymes make cut at specific position in DNA segment.

Which of the following is responsible for the initiation of replication?

  1. Origin of replication

  2. Plasmid

  3. Restriction endonuclease

  4. Vectors

Show answer
Correct Option: A
Explanation:

origin of replication or Ori is responsible for initiation of replication.

The function of exonuclease is

  1. to remove nucleotides from the ends of DNA

  2. to add nucleotides to the ends of DNA

  3. to remove nucleotides at specific position

  4. to add nucleotides at specific position

Show answer
Correct Option: A
Explanation:

Correct ans: Exonuclease enzyme cleaves nucleotides one at a time from the ends of DNA.

Pallindromes are

  1. base pair sequences which are same when read forward and backward

  2. different base pair sequences when read in forward and backward

  3. made of same base pair

  4. not recognised by restriction endonuclease

Show answer
Correct Option: A
Explanation:

Correct ans: Pallindronic sequences are those which are same when read from both the sides. e.g. MALAYALAM, 5'GAATTC3' and 3'CTTAAG 5'. 

Key tools of genetic engineering are all except

  1. Restriction enzymes

  2. Vectors

  3. Polymerase enzymes

  4. Helicase enzymes

Show answer
Correct Option: D
Explanation:

Correct ans: They are involved in unwinding of DNA segment.

The function of DNA LIGASE is

  1. to unwind DNA

  2. to make cuts at specific positions

  3. to form a phosphodiester bond between 5' phosphate group of one nucleotide and 3' hydroxyl group of another nucleotide.

  4. to form a phosphodiester bond between 3' phosphate group of one nucleotide and 5' hydroxyl of other nucleotide.

Show answer
Correct Option: C
Explanation:

Correct.

Alkaline phosphatase has a role in

  1. removing of unwanted nucleotides from the end of DNA

  2. adding of more nucleotides to DNA

  3. the removal of phosphate group

  4. adding of methyl group to one end of DNA

Show answer
Correct Option: C
Explanation:

Correct. As the process of ligation requires the presence of 5' phosphate group, Alkaline phosphatase is used to remove phosphate  and prevent unwanted self ligation.

Which of the following restriction enzymes is used in genetic engineering?

  1. Type one

  2. Type two

  3. Type three

  4. Type four

Show answer
Correct Option: B
Explanation:

Correct: They can be used in vitroto recognise and cut within specific DNA sequence, usually 4-8 nucleotides.

Which enzyme can withstand high temperature (>90 c)?

  1. Thermus aquaticus

  2. Taq polymerase

  3. Cellulase

  4. Lysozyme

Show answer
Correct Option: B
Explanation:

Correct..The enzyme is used in PCR as it can withstand high temperature.

The dye used for staining DNA fragments in electrophoresis is

  1. Ethidium bromide

  2. Ethidium chloride

  3. Ethidium flouride

  4. Ethidium iodide

Show answer
Correct Option: A
Explanation:

Correct . DNA fragments can be seen only after staining with Ethidium Bromide, followed by an exposure to UV radiation as a bright orange coloured band.

Bt cotton is concerned with

  1. herbicide intolerance

  2. low yield

  3. pest resistance

  4. plant height

Show answer
Correct Option: C
Explanation:

Correct

Colchine and Granosan are involved in

  1. inhibition of chromosome replication

  2. increases cell division

  3. prevents the spindle formation

  4. inhibits metaphase

Show answer
Correct Option: C
Explanation:

Correct. These chemicals prevent the spindle formation during mitosis to allow replication of chromosomes.In the absence of the spindle anaphase is blocked.

Brazzein is

  1. an enzyme

  2. a low calorie sweetener

  3. cholestrol reducing

  4. produced by animal

Show answer
Correct Option: B
Explanation:

Correct. This protein is as sweet as sugar and is used as a low calorie swetner

What is the function of Hirudin?

  1. It prevents blood clotting.

  2. It prevents heart attack.

  3. It is produced by bacteria.

  4. It prevents kidney faliure.

Show answer
Correct Option: A
Explanation:

Correct. The protein is present in leech  and prevents blood clotting. Its gene is chemically synthesised and introduced in Brassica napus.

Molecular glue is another name for

  1. DNA Ligase

  2. restriction enzyme

  3. DNA polymerase

  4. Topoisomerase

Show answer
Correct Option: A
Explanation:

Correct. it is used for joining of two DNA fragments.

Transfer of genetic material from one bacterium to another by agency of viruses is called

  1. Transduction

  2. Conjugation

  3. Transformation

  4. Hybridisation

Show answer
Correct Option: A
Explanation:

Correct.

Which of the following statements is correct about plasmids?

  1. They are required by bacteria

  2. They help in reproduction

  3. They are small bacteria

  4. They are self replicating, xtrachromosomal DNA fragnents

Show answer
Correct Option: D
Explanation:

Correct.They are capable of independent replication.

Chemical knives of DNA are

  1. DNA polymerase

  2. Restriction enzyme

  3. RNA synthetase

  4. Reverse transcriptase

Show answer
Correct Option: B
Explanation:

Correct. They make cuts at specific position on DNA fragments.

Electrophoresis means

  1. separation on the basis of charge

  2. separation on the basis of mass

  3. separation on the basis of density

  4. separation on the basis of volume

Show answer
Correct Option: A
Explanation:

correct. Electrophoresis involves migration of negatively charged particle to positively charged electrode and vice versa.

The process of making temporary opening in cell membrane to introduce foreign DNA is

  1. microinjection

  2. particle gun method

  3. electroporation

  4. chemical mediated genetic transformation

Show answer
Correct Option: C
Explanation:

Correct. It is a method to introduce polar molecules into host cell through cell membraneby using electrical impulses.

DNA polymerase was discovered by

  1. A.Korenberg

  2. Paul Berg

  3. A.Tiselius

  4. Cohen & Boyer

Show answer
Correct Option: A
Explanation:

Correct.

Flavr Savr is transgenic variety of

  1. tomato

  2. rice

  3. cotton

  4. wheat

Show answer
Correct Option: A
Explanation:

Correct.it has increased shelf life and better nutrient quality.

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