negative staining

acid fast staining

differential staining

endospore staining

simple staining

New Methylene Blue for reticulocyte staining

Brilliant Cresyl Blue for reticulocyte staining

Trypan blue for eukaryotic cells

Propidium iodide for eukaryotic cells

Both the options 1 and 2

ranging from 1:100 to 1:000

ranging from 1:10 to 1:100

ranging from 1:5000 to 1:500000

ranging from 1:2500 to 1:4000

ratio should be 1:1

Acetone and Ethanol

Crystal violet

Iodine

Fuchsin and safranin

Carbol fuchsin

Iodine

Carbol fuchsin

Acetone and Ethanol

Fuchsin and safranin

Crystal violet

Gram positive become red after counter staining.

Gram negative becomes purple after counter staining.

Gram negative organisms lack secondary membranes and lipopolysaccharide.

Gram positive organisms lack the secondary membrane and lipopolysaccharide.

Gram positive organisms have the secondary membrane and lipopolysaccharide.

Peptidoglycan, found in both Gram positive and negative organisms.

Lipopolysaccharide are found in Gram positive organisms.

Lipopolysaccharide are found in Gram negative organisms.

Teichoic and lipoteichoic acids are found in Gram positive organisms.

Teichoic and lipoteichoic acids are found in Gram negative organisms.

Gram staining

Negative staining

Albert’s staining

Both Gram staining and Albert’s staining

Silver staining

Kinyoun stain

Ziehl–Neelsen stain

Fite stain

Auramine-rhodamine stain

All of the above

Gram staining was invented in the year 1882.

Iodine is used as a mordant in Gram staining.

Gram positive and Gram negative differential helps to take the appropriate antibiotic treatment.

Crystal violet is an anionic charged dye and the exterior of cells have cationic charge.

Aliquot something means “Take a smaller volume of sample.”

Auramine and Rhodamine

Jensen’s

Kopeloff and Beerman’s

Preston and Morrell’s

Weihert’s

gram staining

negative staining

Ziehl-Neelsen staining

Papanicolaou staining

silver staining

Haemotoxylin stains both cell nuclei and red blood cells in red.

Eosin stains both cell nuclei and red blood cells in pink, or bright red.

Haematoxylin stains cell nuclei blue, while Eosin stains red blood cells bright red.

Haematoxylin stains red blood cells in bright red, while Eosin stains cell nuclei blue.

Haemotoxylin and Eosin stains only red blood cells.

Due to presence of higher lipid content, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.

Due to presence of rich level of peptidoglycan, after alcohol-treatment, the porosity of the cell wall increases, hence the CVI complex (Crystal violet -Iodine) can pass through. Thus, the primary stain is not retained.

Primary stain retain after counter stain, violet or purple colour in Gram negative.

Gram negative organisms lacks the secondary membrane and lipopolysaccharide.

Gram negative organisms have teichoic acids or lipoteichoic acids.

an Albert’s staining organism

a gram negative organism

a gram positive organism

acid fast organism

none of these