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Microbiology (GATE)

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The cell membrane of which of the following bacteria contains sterols?

  1. Mycobacteria

  2. Mycoplasma

  3. Micrococcus

  4. Rickettsia


Correct Option: B
Explanation:

The cell membrane of mycoplasma contains sterols. Sterols function as regulators of membrane fluidity during changes in growth temperature or alterations in the fatty acid composition of membrane lipids.

Cytoplasmic membrane of a bacterial cell contains membrane proteins located in various positions. Through specific interactions with phospholipid molecules, which of the following functions is performed by inner-surface proteins?

  1. They provide flexibility to the membrane.

  2. They transport substances across the cytoplasmic membrane.

  3. They transport large molecules into the cells.

  4. They cooperate with other proteins in energy yielding reactions.


Correct Option: D
Explanation:

The inner-surface proteins cooperate with other proteins in energy yielding reactions.

Which of the following ribosomal RNAs is not present in bacterial ribosomes?

  1. 23S

  2. 5S

  3. 18S

  4. 16S


Correct Option: C
Explanation:

18S rRNA is not present in bacterial ribosomes. It is found in eukaryotic ribosomes.

Which of the following cellular structures are used for the identification of bacterium Corynebacterium diphtheriae?

  1. Volutin granules

  2. Carboxysomes

  3. Magnetosomes

  4. Microcompartments


Correct Option: A
Explanation:

Volutin granule production is used for the identification of bacterium Corynebacterium diphtheriae. These are either stained by Albert's stain and viewed microscopically or they produce distinct colonies on Loffler's medium.

Which of the following are the polyhedral membrane-bound compartments in the bacterial cells that are made up of a protein shell?

  1. Gas vesicles

  2. Volutin granules

  3. Carboxysomes

  4. Microcompartments


Correct Option: D
Explanation:

Microcompartments are the polyhedral membrane bound compartments in the bacterial cell that are made up of a protein shell and enclose various enzymes. These polyhedral organelles localise and compartmentalise bacterial metabolism, a function performed by the membrane-bound organelles in eukaryotes.

The position of endospores differs among bacterial species. Endospores are centrally placed in which of the following organisms?

  1. Clostridium tetani

  2. Bacillus cereus

  3. Bacillus subtilis

  4. Salmonella typhi


Correct Option: B
Explanation:

Bacillus cereus has centrally placed endospores.

Which of the following statements about enzyme DD-transpeptidase is true?

  1. It provides antigenicity to the cell wall.

  2. It makes the cell wall semi-permeable.

  3. It makes the cell wall strong and rigid.

  4. It breaks the amino acid linkages in the cell wall.


Correct Option: C
Explanation:

DD-transpeptidase helps in cross linking between amino acids in different linear sugar amino chains. It results in a 3-dimensional structure that is strong and rigid.

Fimbriae are a type of extracellular structures found in some bacterial cells. Myxococcus uses fimbriae for

  1. attachment

  2. motility

  3. sexual reproduction

  4. photoreception


Correct Option: B
Explanation:

Myxococcus uses fimbriae for motility. It facilitates the assembly of multicellular structures.

Which of the following statements about bacterial flagella are false?

a. They operate at high Reynolds number. b. Sodium-ion pump driven flagella are found in vibrio. c. Flagella evolve from Type III secretory system. d. The S-ring of flagella directly attaches to plasma membrane. e. Campylobacter jejuni has 11 protofilaments. f. Fasciculi are seen in spirochetes. g. Archeal flagella grow by addition of subunits to the base.

  1. a, b and d

  2. e, f and g

  3. a, e and f

  4. e, b and c


Correct Option: C
Explanation:

a, e and f are false. Bacterial flagella operate at low Reynolds number. Campylobacter jejuni has 7 protofilaments. Fasciculi are seen in Selanomonas.

Which of the following organelles of a bacterial cell acts as partial permeability barrier for large substrates?

  1. Plasma membrane

  2. Cell wall

  3. S-layer

  4. Glycocalyx


Correct Option: C
Explanation:

S-layer acts as a partial permeability barrier for large substrates. It keeps extracellular proteins near the cell membrane by preventing their diffusion away from the cell.

Bacteria conjugative pili are absent in

  1. Rickettsia felis

  2. Bacillus subtilis

  3. E. coli

  4. Brevibacterium mcbrellneri


Correct Option: B
Explanation:

Bacillus subtilis is a Gram-positive bacillus and pili are absent in Gram-positive bacteria. They possess pili-like proteins or Type 4 pilins which assemble into pili (long polymeric fibers that function in bacterial adhesion and motility).

In Gram-negative cells, M-ring of flagella is embedded in

  1. plasma membrane

  2. peptidoglycan layer

  3. lipopolysaccharide

  4. cytoplasm


Correct Option: A
Explanation:

In Gram-negative cells, M-ring of flagella is embedded in plasma membrane.

In a Gram-positive cell wall, the structure responsible for providing antigenicity to the organism is

  1. lipoteichoic acid

  2. teichoic acid

  3. lipopolysaccharide

  4. peptide chain


Correct Option: A
Explanation:

Lipoteichoic acid confers antigenic properties to Gram-positive organisms. It stimulates specific immune responses and is released from the bacterial cells mainly after bacteriolysis is induced by lysozymes, cationic peptides from leucocytes or beta-lactam antibiotics.

Fimbriae are found in which of the following?

  1. Fusobacteria

  2. Actinobacteria

  3. Proteobacteria

  4. Fermicules


Correct Option: C
Explanation:

Fimbriae are present in most members of Proteobacteria.

Which of the following amino acids is not present in a Gram-negative cell wall?

  1. L-alanine

  2. D-alanine

  3. Meso-Diaminopimelic acid

  4. D-glutamic acid


Correct Option: B
Explanation:

D-alanine is present only in Gram-positive cell walls.

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