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Landmark Discoveries in Microbiology

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Number of Questions: 15
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Tags: koch pasteur Jenner Landmark Discoveries Relevant to the Field of Microbiology
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Animals and plants, complex as they may appear, are yet composed of a few elementary parts frequently repeated.

Who gave the above statement?

  1. Robert Hooke

  2. Aristotle

  3. Roger Bacon

  4. Kircher


Correct Option: B
Explanation:

Aristotle quoted the given statement in description of the cellular structure of living things back in the 4th century B.C.

Whose discovery in 1897 refuted Pasteur's claim that fermentation requires the presence of live cells?

  1. Waldemar Haffkine

  2. Edward Buchner

  3. Paul Ehrlich

  4. Almwroth Wright and David Sample


Correct Option: B
Explanation:

Edward Buchner published the first evidence of a cell-free fermentation process using extracts isolated from yeast. This discovery refuted Pasteur's claim that fermentation requires the presence of live cells.

Which of the following periods is known as the golden era of microbiology?

  1. 1892-1902

  2. 1882-1891

  3. 1865-1882

  4. 1903-1917


Correct Option: C
Explanation:

The period between 1865-1882 is known as the golden era of microbiology because all the pioneering works of Louis Pasteur, Robert Koch and Lister etc. made the most important discoveries and laid the foundation of microbiology.

Which of the following is known as Pasteur effect?

  1. Disproving the doctrine of spontaneous generation

  2. Use of artificially weakened pathogens for immunization

  3. Production of alcohol in absence of oxygen by yeast

  4. Elimination of causative agent of pebrine disease


Correct Option: C
Explanation:

Louis Pasteur introduced the terms aerobic and anaerobic in describing the growth of yeast at the expense of sugar in the presence or absence of oxygen. He observed that more alcohol was produced in the absence of oxygen when sugar is fermented, which is now termed the Pasteur effect.

Which of the following pathogens was not discovered by Robert Koch?

  1. Mycobacterium tuberculosis

  2. Mycobacterium leprae

  3. Bacillus anthracis

  4. Vibrio cholerae


Correct Option: B
Explanation:

Gerhard Henrik Armauer Hansen discovered Mycobacterium leprae in 1873. This was the first time that a chronic infectious disease in humans was shown to be related to a bacillus.

The first prototype of the present day compound microscope was given by

  1. Zacharias Janssen

  2. Conard Gesber

  3. Galileo

  4. Robert Hooke


Correct Option: A
Explanation:

The first prototype of the present day compound microscope was given by Zacharias Janssen by adding one more lens to the telescope.

For which of the following are Schwann and Schulze known?

  1. Pure cultures

  2. Cotton plugs

  3. Agar powder

  4. Bacteriology


Correct Option: B
Explanation:

Schwann and Shculze are known for introducing cotton plugs to prevent entry of microorganism in sterile medium.

In 1942, who demonstrated that an E. coli T2 phage has a head and a tail?

  1. Salvador Luria and Max Delbruck

  2. Thomas Anderson and Salvador Luria

  3. Salvador Luria and Alfred Day Hershey

  4. Andre Lwoff and Louis Siminovitch


Correct Option: B
Explanation:

In 1942, Thomas Anderson and Salvador Luria demonstrated that an E. coli T2 phage has a head and a tail. They photographed bacteriophages with the aid of an electron microscope.

Fluctuation analysis given by Salvador Luria and Max Delbruck is associated with

  1. gene mapping

  2. mutation

  3. minimum inhibitory concentration

  4. linkage


Correct Option: B
Explanation:

Fluctuation analysis given by Salvador Luria and Max Delbruck is associated with mutation rate. Fluctuation analysis shows that resistance in bacteria occurs before exposure to the phage and argues against the adaptation hypothesis of mutations.

In 1995, Craig Venter, Hamilton Smith, Claire Fraser and colleagues at TIGR (The Institute for Genomic Research) elucidated the first complete genome sequence of a microorganism. Which was the organism whose complete genome sequence was elucidated?

  1. Zymomonas mobilis

  2. Burkholderia pseudomallei

  3. Haemophilus influenzae

  4. Mycobacterium tuberculosis


Correct Option: C
Explanation:

Haemophilus influenzae was the first microorganism whose genome was sequenced. In 1995, Craig Venter, Hamilton Smith, Claire Fraser and colleagues at TIGR (The Institute for Genomic Research) elucidated the first complete genome sequence of Haemophilus influenzae.

Which organism was used by George Beadle and Edward Tatum to establish one gene one enzyme concept?

  1. Actinomycetes

  2. Drosophila melanogaster

  3. Neurospora fungus

  4. Streptococcus pneumoniae


Correct Option: C
Explanation:

George Beadle and Edward Tatum in 1941 using the fungus Neurospora crassa established the one gene one enzyme concept. According to it, particular genes are expressed through the action of correspondingly specific enzymes.

Arrange the following discoveries in chronological order:

a. First picture of virus b. Viral growth cycle against E. coli c. Field trials of vaccine against yellow fever virus d. Isolation of RNA from TMV

  1. a, c, d, b

  2. d, b, a, c

  3. d, c, b, a

  4. d, a, b, c


Correct Option: C
Explanation:

This is the correct chronological sequence. In 1936, J. D. Bernal, F. C. Bauden, N. W. Pirie isolated ribonucleic acids from TMV. In 1938, Max Theiler's vaccine against yellow fever was proved successful in field trials. In 1939, E. L. Ellis and Max Delbruck established the concept of one-step viral growth cycle for a bacteriophage active against E. coli and in 1940, Helmuth Ruska used an electron microscope to obtain the first pictures of a virus.

In 1889, Kitosato obtained first pure culture of an anaerobe. Pure culture of which anaerobe was obtained by him?

  1. Clostridium botulinum

  2. Clostridium tetani

  3. Clostridium perfringens

  4. Bacteroides


Correct Option: B
Explanation:

In 1889, Kitasato obtained the first pure culture of the strict anaerobic pathogen, the tetanus bacillus Clostridium tetani.

Who published an early classification of bacteria and used the genus name Bacillus for the first time?

  1. D. H. Bergey

  2. Ferdinand J. Cohn

  3. Brefeld

  4. Thomas Burrill


Correct Option: B
Explanation:

In 1875, Cohn published an early classification of bacteria, using the genus name Bacillus for the first time.

Who discovered the process of intermittent heating to kill spores?

  1. Louis Pasteur

  2. Joseph Lister

  3. Griffith Evans

  4. John Tyndall


Correct Option: D
Explanation:

John Tyndall developed a process of intermittent heating to kill spores known as Tyndallization. Tyndallization essentially consists of heating the substance to boiling point (or just a little below boiling point) and holding it there for 15 minutes, three days in succession. After each heating, the resting period will allow spores that have survived to germinate into bacterial cells; these cells will be killed by heating the next day.

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