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Genetics and Evolution

Description: This test is based on various topics from Genetics and Evolution and thus it will help the students to revise the topic thoroughly.
Number of Questions: 50
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Tags: Genome sequencing Theories and Evidences of Evolution Theory of Evolution of Life Forms Cytoplasmic Inheritance Genetics and Evolution Linkage and Crossing Over Linkage and Recombination of Genes
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Which of the following types of epistatis takes place when one mutation has the opposite effect in the spectre of another mutation?

  1. Additivity

  2. Positive epistasis

  3. Negative epistasis

  4. Genetic enhancement

  5. Sign epistasis


Correct Option: E
Explanation:

Sign epistasis occurs when one mutation has the opposite effect when in the presence of another mutation. This occurs when a mutation that is deleterious on its own can enhance the effect of a particular beneficial mutation. For example, a large and complex brain is a waste of energy without a range of sense organs, however sense organs can be more useful if the organisms brain is better able to process the information.

Who discovered the first sex-linked gene?

  1. Leonard Doncaster

  2. Gwynaeth Bodmer

  3. Fisher

  4. John Anthony Hardy

  5. Karen P. Steel


Correct Option: A
Explanation:

The first sex-linked gene ever discovered was the lacticolor X-linked recessive gene in the moth Abraxas grossulariata by Leonard Doncaster.

Which of the following statements is false regarding epistasis?

  1. Epistasis has a small influence on the shape of evolutionary landscapes.

  2. Epistasis within the genomes of organisms occurs due to interactions between the genes within the genome.

  3. Negative epistasis and sex are thought to be intimately correlated.

  4. Epistasis is measured as the difference between the effects of the mutations together versus the sum of their individual effects.

  5. Epistasis in diploid organisms is further complicated by the presence of two copies of each gene.


Correct Option: A
Explanation:

Epistasis has a large influence on the shape of evolutionary landscapes, which leads to profound consequences for evolution and evolvability of traits.

In a married couple, an unaffected woman carries one copy of a gene mutation for an X-linked recessive disorder. She has an affected son, an unaffected daughter who carries one copy of the mutation. Which among the following X-linked recessive disorders are caused by a mutation of the Factor VIII gene in human beings?

  1. Red-green colour blindness

  2. Hemophilia A

  3. Hemophilia B

  4. X-linked agammaglobulinemia

  5. X-linked ichthyosis


Correct Option: B
Explanation:

Hemophilia A is a blood clotting disorder caused by the mutation of factor VIII gene and leads to the deficiency of factor VIII. It was once thought to be the royal disease found in the descendants of Queen Victoria.

cDNA is known to be synthesised, or manufactured from an mRNA or messenger RNA template. Which of the following statements regarding the synthesis of complementary DNA (cDNA) is incorrect?

  1. It is likely that the ssDNA forms a hairpin loop at the 3' end.

  2. DNA polymerase is added along with deoxynucleotide triphosphates.

  3. A poly-T oligonucleotide primer is hybridised onto the poly-A tail of the mature mRNA template.

  4. The mixture of mature mRNA strands is extracted from the cell.

  5. A eukaryotic cell transcribes the DNA into RNA.


Correct Option: B
Explanation:

Reverse transcriptase is added along with deoxynucleotide triphosphates (A, T, G, C). This synthesises one complementary strand of DNA hybridised to the original mRNA strand.

As a general rule, the DNA of most organisms is negatively supercoiled. Which of the following DNA replication enzymes regulates the negative supercoiling of the DNA molecule?

  1. Helicases

  2. DNA polymerases

  3. Topoisomerases

  4. DNA clamp

  5. DNA gyrase


Correct Option: E
Explanation:

DNA gyrase relieves strain while double-strand DNA is being unwound by helicase. This causes negative supercoiling of the DNA.

Sex linkage in genetics is a condition in which a particular gene is located on a sex chromosome. Baldness in humans is an example of which of the following types of sex linkages?

  1. X-linked dominant

  2. X-linked recessive

  3. Y-linkage

  4. Sex-influenced

  5. Sex-limited


Correct Option: D
Explanation:

Sex-influenced or sex-conditioned traits are phenotypes affected by whether they appear in a male or female body. Even in a homozygous dominant or recessive female, the condition may not be expressed fully. Example: Baldness in humans.

After researching a lot, the geneticist concluded that the given specimen has undergone a mutatiom in which the original phenotype of an organism is restored. In which of the following mutations is the original phenotype of an organism is restored?

  1. Gain-of-function mutations

  2. Loss-of-function mutations

  3. Antimorphic mutations

  4. A back mutation

  5. Lethal mutations


Correct Option: D
Explanation:

A back mutation or reversion is a point mutation that restores the original sequence and hence the original phenotype.

The mutation leading to the formation of a premature stop codon is

  1. Frameshift mutation

  2. Nonsense mutation

  3. Missense mutations

  4. Silent mutations

  5. Neutral mutation


Correct Option: B
Explanation:

A nonsense mutation is a point mutation in a sequence of DNA that results in a premature stop codon or a nonsense codon in the transcribed mRNA, and possibly a truncated and often non functional protein product.

Which among the following statements does NOT characterise sex linkage in mammals?

  1. Sex linkage is the genotypic expression of an allele related to the chromosomal sex of the individual.

  2. Genes on the X or Y chromosome are called sex-linked.

  3. X-linked recessive allele in humans causes hemophilia.

  4. X-linked traits are maternally inherited.

  5. The incidence of recessive X-linked phenotypes in females is the square of that in males.


Correct Option: A
Explanation:

Sex linkage is the phenotypic expression of an allele related to the chromosomal sex of the individual. This mode of inheritance is in contrast to the inheritance of traits on autosomal chromosomes, where both sexes have the same probability of inheritance.

Which among the following statements regarding the Okazaki fragments is incorrect?

  1. Okazaki fragments are formed on the lagging template strand during DNA replication.

  2. They are separated by ~10-nucleotide RNA primers and are unligated until RNA primers are removed.

  3. On the leading strand DNA replication proceeds continuously along the DNA molecule.

  4. DNA is synthesised from 3' to 5' end.

  5. There are two pathways that have been proposed to process Okazaki fragments.


Correct Option: D
Explanation:

DNA is synthesised from 5' to 3', thus when copying the 3' to 5' strand, replication is continuous. Phosphodiester links form between the 3' to 5' and nucleotides can be added with the aid of the enzyme DNA polymerase for the continuous leading strand.

Premature translation termination mediates triosephosphate isomerase mRNA degradation. Which one of the following types of mutations is most likely to lead to premature termination of translation?

  1. Exon skip

  2. Single base change in a promoter

  3. Deletion of an entire gene

  4. Deletion of three bases from DNA

  5. Insertion of a single base into DNA


Correct Option: E
Explanation:

Insertion of one base will alter the reading frame and usually leads to a stop codon eventually.

Various enzymes are involved in the formation of the DNA fragment. Which among the following enzymes is responsible for synthesising Okazaki fragments from 5’ to 3'?

  1. Primase

  2. DNA polymerase δ

  3. DNA ligase

  4. Flap endonuclease 1

  5. Dna2 endonuclease


Correct Option: A
Explanation:

Primase adds RNA primers onto the lagging strand, which allows synthesis of Okazaki fragments from 5’ to 3’. However, Primase creates RNA primers at a much lower rate than that at which DNA polymerase synthesizes DNA on the leading strand.

DNA replication occurs when a strand of DNA produces a copy of itself. Which among the following statements regarding DNA replication is FALSE?

  1. Okazaki fragments are formed on the lagging strand.

  2. The lagging strand usually contains longer stretches of single-stranded DNA.

  3. Each Okazaki fragment is preceded by an RNA dimer.

  4. Two replicative polymerases synthesize DNA in opposite orientations.

  5. Duplex DNA is always antiparallel.


Correct Option: C
Explanation:

Each Okazaki fragment is preceded by an RNA primer which is displaced by the procession of the next Okazaki fragment during synthesis.

Which of the following is most likely to be a conservative mutation?

  1. CAA to TAA

  2. CCT to GCT

  3. ATA to AAA

  4. ATC to GTC

  5. CGA to TGA


Correct Option: D
Explanation:

With reference to the genetic code, the conversion of ATC to GTC is isoleucine to valine,  both are nonpolar amino acids. Therefore, this substation is most likely to be conservative.

A spliceosome is a large and complex molecular machine found primarily within the splicing speckles of the cell nucleus of eukaryotic cells. Which one of the following types of RNA is included in the splicosome?

  1. snRNA

  2. miRNA

  3. tRNA

  4. siRNA

  5. rRNA


Correct Option: A
Explanation:

The spliceosome is assembled from snRNAs and protein complexes. The spliceosome removes introns from a transcribed pre-mRNA, a kind of primary transcript. snRNA (small nuclear RNA) is a component of the splicosome.

Down syndrome is a chromosomal condition that is associated with intellectual disability, a characteristic facial appearance, and weak muscle tone. The structural abnormalities of chromosomes associated with Patau syndrome and Down syndrome is/are

  1. inversions

  2. Isochromosome

  3. Robertsonian translocation

  4. duplications

  5. Deletions


Correct Option: C
Explanation:

A Robertsonian translocation in balanced form results in no excess or deficit of genetic material and causes no health difficulties.

Obesity genetics has been predominantly driven by research into monogenic or syndromic obesity. Which of the following statements does NOT characterise monogenic obesity?

  1. The receptor gene mutations might be one of the reasons for severe obesity.

  2. In Leptin melanocortin pathway, leptin crosses the blood brain barrier and triggers the neurons present in the hypothalamus to generate peptides.

  3. The phenotype is directly and strongly related to the genotype.

  4. The common monogenic obesity disorder was represented by excess of MC4R .

  5. It occurs due to the congenital leptin deficiency.


Correct Option: D
Explanation:

The mutation in the melanocortin-4 receptor protein (MC4R) which is a component of leptin-melanocortin pathway was detected to be related to obesity. The common monogenic obesity disorder was represented by MC4R deficiency. The obesity was accounted due to homozygous and heterozygous mutations in MC4R.

Which of the following genetic disorders is monosomic?

  1. Dermoid tumors of the ovary

  2. Turner syndrome

  3. Hydatidiform mole

  4. Klinefelter syndrome

  5. Fragile X syndrome


Correct Option: B
Explanation:

A monosomic disorder results when a chromosome pair fails to separate during either meiosis 1 or 2, a process known as nondisjunction. Nondisjunction can result in monosomy or trisomy. Turner syndrome occurs when part or all of the second sex chromosome is absent, resulting in a 45X karyotype.

The theories of Charles Darwin still form the foundation for our understanding of natural selection. It is still widely upheld that natural selection is based on five factors. Which of the following factors regarding natural selection is incorrect?

  1. Within a species, individual variations exist naturally.

  2. The number of offsprings produced are more than that can survive.

  3. The reproductive rates of individuals are unequal.

  4. Environmental conditions determine the reproductive success of certain individuals.

  5. The process in which the frequency of certain traits within a species change by even reproduction rates caused by natural selection is called evolution.


Correct Option: E
Explanation:

Individuals who are able to provide the necessary food, shelter and avoid predation reproduce more successfully than others. Over generations, the characteristics of the population change as those individuals more successful in reproduction populate the species. The process in which the frequency of certain traits within a species change by uneven reproduction rates caused by natural selection is called evolution.

Which of the following statements regarding the lac operon is INCORRECT?

  1. The first control mechanism is the regulatory response to lactose.

  2. Transcription of all genes starts with the binding of the enzyme RNA polymerase.

  3. Specific control of the lac genes depends on the availability of the substrate lactose to the bacterium.

  4. The repressor is an allosteric protein.

  5. The lac repressor is a trimer of identical subunits.


Correct Option: E
Explanation:

The lac repressor is a tetramer of identical subunits. Each subunit contains a helix-turn-helix (HTH) motif capable of binding to DNA. The operator site where repressor binds is a DNA sequence with inverted repeat symmetry.

Which of the following statements regarding the Darwin's theory of evolution by natural selection is false?

  1. Darwin set out his theory of evolution by natural selection.

  2. Darwin thought of natural selection by analogy to how farmers select crops or livestock for breeding.

  3. The struggle for existence was later described by Islamic writer Al-Jahiz in the 9th century.

  4. Darwin published a detailed account of his evidence and conclusions in On the Origin of Species in 1859.

  5. Darwin's ideas were inspired by the work of Patrick Matthew.


Correct Option: E
Explanation:

Darwin's ideas were inspired by the observations that he had made on the Beagle voyage, and by the work of a political economist, the Reverend Thomas Malthus, who in An Essay on the Principle of Population, noted that population (if unchecked) increases exponentially, whereas the food supply grows only arithmetically; thus, inevitable limitations of resources would have demographic implications leading to a struggle for existence.

The activator proteins frequently binds with the pertinent DNA segment in which of the following types of operons?

  1. Neutral repressible operons

  2. Negative repressible operons

  3. Negative inducible operons

  4. Positive repressible operons

  5. Positive inducible operons


Correct Option: D
Explanation:

In positive repressible operons, the activator proteins are normally bound to the pertinent DNA segment. However, when an inhibitor is bound by the activator, it is prevented from binding the DNA. This stops activation and transcription of the system.

Which of the following genetic disorders is expressed in heterozygotic condition in humans?

  1. Sickle Cell Anemia

  2. Brachydactyly

  3. Tay-Sachs disease

  4. Cystic Fibrosis

  5. Congenital Ichthyosis


Correct Option: B
Explanation:

It is a genetic state in which the fingers are unusually short in heterozygotic condition. But, this condition is lethal during early years to homozygous recessive individuals due to major skeletal defects.

Which of the following are derived from the hybridisation of parents with dissimilar genomic sets?

  1. Neopolyploids

  2. True polyploids

  3. Complete allopolyploids

  4. Segmental allopolyploids

  5. Autoallopolyploids


Correct Option: B
Explanation:

True or genomic polyploids are derived from the hybridisation of parents with dissimilar genome sets.

A recombination in the formation of the male gametes ends in which type of genetic disorder?

  1. 45, X Turner syndrome

  2. Mixed gonadal dysgenesis

  3. XX male syndrome

  4. 47, XYY

  5. 47, XXY


Correct Option: C
Explanation:

XX male syndrome occurs when there has been a recombination in the formation of the male gametes, causing the SRY-portion of the Y chromosome to move to the X chromosome. When such an X chromosome contributes to the child, the development will lead to a male because of the SRY gene.

Which of the following variable traits are influenced by a combination of genes and environmental factors?

  1. Freckles

  2. Handedness

  3. Dimples

  4. Tongue rolling

  5. Earlobe attachment


Correct Option: D
Explanation:

Some people can curl up the sides of their tongue to form a tube shape. Tongue rolling is controlled by a single gene. However, people can learn to roll their tongue as they get older, suggesting that environmental factors also influence the trait.

A dominantly inherited trait affects a newborn baby of Verma family and his grandmother, but neither parent. Which of the following principles does it illustrate?

  1. Variable expressivity

  2. Somatic mosaicism

  3. Germline mosaicism

  4. Non-penetrance

  5. Somatic mutations


Correct Option: D
Explanation:

Non-penetrance means that a person has inherited the genetic change, but that it does not manifest the associated phenotype. A skipped generation for a dominant trait is an example of non-penetrance.

A pregnant woman is known to be at risk for autosomal dominant polycystic kidney disease and requests a genetic test to determine if she has inherited the mutation from his affected mother. At the time of testing, she is asymptomatic, but the condition displays age-dependent penetrance. Which of the following types of genetic testing method illustrate the woman's condition?

  1. Pharmacogenetic testing

  2. Predisposition testing

  3. Presymptomatic testing

  4. Diagnostic testing

  5. Predictive testing


Correct Option: C
Explanation:

Presymptomatic testing involves determining whether an individual has inherited a gene mutation that exhibits age-dependent penetrance.

Uniparental disomy for chromosome 15 in which both chromosomes are maternally derived is associated with which of the following syndromes?

  1. Prader–Willi syndrome

  2. Angelman syndrome

  3. Rett syndrome

  4. Beckwith-Wiedemann syndrome

  5. Cystic fibrosis


Correct Option: A
Explanation:

Maternal uniparental disomy (UPD) for chromosome 15 results in Prader–Willi syndrome; paternal UPD would lead to Angelman syndrome.

Which of the following human traits is a result of natural selection?

  1. Hitchhiker's thumb

  2. Skin colour

  3. Widow's peak

  4. Tounge rolling

  5. Eye colour


Correct Option: B
Explanation:

Human skin colour ranges in variety from the darkest brown to the lightest pinkish-white hues. Human skin pigmentation is the result of natural selection. Skin pigmentation in human beings evolves primarily to regulate the amount of ultraviolet radiation penetrating the skin, controlling its biochemical effects.

The doctor told a couple that their new born baby is suffering from a metabolic disorder caused by a mutation in the DHCR7 gene on chromosome 11. Which of the following disorders could the doctor be referring to?

  1. Smith-Lemli-Opitz syndrome

  2. Severe Combined Immunodeficiency

  3. Sickle cell anaemia

  4. Neurofibromatosis type 1

  5. Phenylketonuria


Correct Option: A
Explanation:

SLOS Chromosome SLOS is a metabolic disorder caused by a mutation in the DHCR7 (7-dehydrocholesterol reductase) gene on chromosome 11. This gene codes for an enzyme that is involved in the production of cholesterol. People who have SLOS are unable to produce enough cholesterol to support normal growth and development.

The Y chromosome is the sex-determining chromosome in many species. The following chromosomal states are associated with changes in the Y chromosome. Which of the following genetic disorders occurs due to the swapping of genetic material between chromosomes?

  1. 47, XXY syndrome

  2. Y chromosome infertility

  3. 47, XYY syndrome

  4. 48, XXYY syndrome

  5. 46, XX syndrome


Correct Option: E
Explanation:

This state results from an abnormal swap over of genetic material between chromosomes. This swap over occurs as a chance event in the father of an affected person during the formation of sperm cells. The translocation affects the gene in charge that is SRY for development of a foetus into a male.

In which of the following mutations the two chromosomes interchange their chromosome segments leading to certain genetic disorders?

  1. Aneuploidy

  2. Translocations

  3. Deletion

  4. Inversions

  5. Duplications


Correct Option: B
Explanation:

This type of mutation takes place between two chromosomes and interchanges their chromosome segments. In other words, a portion of chromosome is transferred on to another chromosome.

Pseudogenes, also known as noncoding DNA, are extra DNA in a genome that do not get transcribed into RNA to synthesise proteins. Which of the following statements regarding the pseudogenes is FALSE?

  1. Exact mapping of the pseudogenes onto chromosomes is extremely difficult.

  2. The absolute rate of mutation is lower in nucleus than the one occurring in mitochondria.

  3. They form a molecular record of the evolutionary history and statistics of the parental genome.

  4. Multiple pseudogenes of the same sequence are found in human genes.

  5. They are also useful to find the rate of nucleotide substitutions, DNA loss, etc.


Correct Option: C
Explanation:

They form a molecular record of the evolutionary history and statistics of the parental genome.

Pigmentation is highly heritable, being regulated by genetic, environmental, and endocrine factors that modulate the amount, type and distribution of melanins in the skin, hair and eyes. Which of the following genes regulates calcium in melanocytes ?

  1. MC1R gene

  2. KITLG gene

  3. TYR gene

  4. SLC45A2 gene

  5. SLC24A5


Correct Option: E
Explanation:

This gene regulates calcium in melanocytes and is important in the process of melanogenesis.

DNA damage caused by exogenous agents comes in various forms. Which of the following agents increases the rate of depurination in DNA strands?

  1. Vinyl chloride

  2. Ionising radiation

  3. High tempretures

  4. UV-A light

  5. UV-B light


Correct Option: C
Explanation:

Thermal disruption at elevated temperature increases the rate of depurination (loss of purine bases from the DNA backbone) and single-strand breaks. For example, hydrolytic depurination is seen in the thermophilic bacteria, which grow in hot springs at 40-80 °C.

Which of the following genetic processes is used to identify the effect of promoters' activity on the transcription of downstream genes?

  1. Molecular cloning

  2. Promoter bashing

  3. Gel electrophoresis

  4. Site-directed mutagenesis

  5. Restriction digest


Correct Option: B
Explanation:

Promoter bashing is a technique used in molecular biology to identify how certain regions of a DNA strand, commonly promoters, affect the transcription of downstream genes.

Which of the following genetic diseases is not inherited?

  1. 1p36 deletion syndrome

  2. 45, X

  3. 47, XXY

  4. 47, XX, + 21

  5. 47, XYY


Correct Option: E
Explanation:

47, XYY is not inherited, but usually occurs as a random event during the formation of sperm cells. An incident in chromosome separation during anaphase II (of meiosis II) called non disjunction can result in sperm cells with an extra copy of the Y-chromosome. If one of these atypical sperm cells contributes to the genetic makeup of a child, the child will have an extra Y-chromosome in each of the body's cells.

Which of the following statements does NOT characterise the process of natural selection?

  1. Variation exists within all populations of organisms.

  2. Natural selection acts on the genotype of an organism.

  3. Natural variation occurs among the individuals of any population of organisms.

  4. The concept of fitness is central to natural selection.

  5. Natural selection acts on individuals.


Correct Option: B
Explanation:

Natural selection acts on the phenotype or the observable characteristics of an organism, but the genetic (heritable) basis of any phenotype that gives a reproductive advantage may become more common in a population.

In a cell, which of the following gene expressions is most susceptible to environmental changes?

  1. A housekeeping gene

  2. A Hox genes

  3. A constitutive gene

  4. An inducible gene

  5. A facultative gene


Correct Option: D
Explanation:

An inducible gene is a gene whose expression is either susceptible to environmental change or dependent on the position in the cell cycle.

Which of the following acts as the inducer of the lac operon?

  1. Phosphoenolpyruvic acid

  2. Allolactose

  3. ONPG

  4. Phenyl-β-D-galactose (phenyl-Gal)

  5. Isopropyl-β-D-thio-galactoside


Correct Option: B
Explanation:

Allolactose is an isomer of lactose and is the inducer of the lac operon. Lactose is galactose-(β1->4)-glucose, whereas allolactose is galactose-(β1->6)-glucose. Lactose is converted to allolactose by β-galactosidase in an alternative reaction to the hydrolytic one.

Which of the following DNA-modifying enzymes is regarded as a type of molecular motor?

  1. Nucleases

  2. Polymerases

  3. Helicases

  4. Topoisomerases

  5. DNA ligases


Correct Option: C
Explanation:

Helicases are proteins that are a type of a molecular motor. They use the chemical energy in nucleoside triphosphates, predominantly ATP, to break hydrogen bonds between bases and unwind the DNA double helix into single strands. These enzymes are essential for most processes where enzymes need to access the DNA bases.

Which of the following semiessential amino acids is encoded by six different codons in human beings?

  1. Methionine

  2. Tryptophan

  3. Arginine

  4. Leucine

  5. Serine


Correct Option: C
Explanation:

Arginine is an α-amino acid. At the level of molecular genetics, in the structure of the messenger ribonucleic acid mRNA, CGU, CGC, CGA, CGG, AGA, and AGG are the triplets of nucleotide bases or codons that code for arginine during protein synthesis.

Mutations in the genes for the hemoglobin protein in a particular species leads to the formation of hemoglobin variants. Which of the following genetic disorders is caused by mutation in the haemoglobin gene?

  1. Haemophilia

  2. Tay-Sachs disease

  3. Sickle-cell disease

  4. Cystic fibrosis

  5. Phenylketonuria


Correct Option: C
Explanation:

Sickle-cell disease is a hereditary blood disorder, characterised by red blood cells that assume an abnormal, rigid and sickle shape. Sickling decreases the cells' flexibility and results in a risk of various life-threatening complications. This sickling occurs because of a mutation in the haemoglobin gene.

Centromere proteins is a group of proteins which either forms or mediates the function of centromeres. The centromere protein predominately essential for keeping proper kinetochore size is

  1. Centromere protein F

  2. Centromere-associated protein E

  3. Centromere protein I

  4. Centromere protein B

  5. Centromere protein C 1


Correct Option: E
Explanation:

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12.

There are certain mechanisms involved in the origin of new genes. Which of the following mechanisms occurs between the genomes of the cell organelles like mitochondria, chloroplasts and the nuclear genomes?

  1. Lateral Gene Transfer

  2. Transposable Element(TE) protein domestication

  3. Gene Fusion and Fison

  4. De Novo Gene Origination

  5. Gene duplication


Correct Option: A
Explanation:

It is referred to the situation in which a gene does not have a vertical origin (that is direct inheritance from parent to offspring) but comes from an unrelated genome. It is recognised that this type of transfer occurs between bacteria, and that it also has taken place between the genomes of the cell organelles like mitochondria, chloroplasts and the nuclear genomes.

There are numerous techniques in cytogenetics to investigate the different features of chromosomes within a cell. Which of the following is a frequently used fluorescent pattern acquired employing quinacrine for staining the chromosomes?

  1. T-banding

  2. Q-banding

  3. G-banding

  4. R-banding

  5. C-banding


Correct Option: B
Explanation:

Q-banding is a fluorescent pattern obtained using quinacrine for staining. The pattern of bands is very similar to that seen in G-banding. They can be recognised by a yellow fluorescence of differing intensity. Most part of the stained DNA is heterochromatin. Quinacrin (atebrin) binds both regions rich in AT and in GC, but only the AT-quinacrin-complex fluoresces.

Chromatin is a complex of DNA and proteins that forms chromosomes within the nucleus of eukaryotic cells. The histone protein significant in identifying the complete structure of chromatin is

  1. Histone H4

  2. Histone H3

  3. Histone H2B

  4. Histone H2A

  5. Histone H1


Correct Option: D
Explanation:

H2A is important for packaging DNA into chromatin. Since H2A packages DNA molecules into chromatin, the packaging process will effect the gene expression. H2A has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin.

In plants, the transcription of Cab genes has been previously shown to be regulated by a circadian oscillator coupled to the red light-absorbing plant photoreceptor. Which among the following types of oscillating gene present in plants regulates the expression of CAB?

  1. Kai genes

  2. The CCA1 gene

  3. Frq gene

  4. The LHY gene

  5. Toc1 gene


Correct Option: E
Explanation:

Toc1 gene, also known as Timing of CAB Expression 1 gene, is an oscillating gene found in the plants that is known to control the expression of CAB. It has been shown to affect the period of circadian rhythms through its repression of transcription factors. This was found through mutations of toc1 in plants that had shortened period of CAB expression.

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