Biotechnology and Its Applications (Class XII) Online Test

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Biotechnology and Its Applications (Class XII)

Description: Biology-Biotechnology
Number of Questions: 15
Created by: Gagan Singh
Tags: Biology-Biotechnology Biotechnology and its Applications

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HaeIII breaks DNA at 5'-GGCC-3'. Which fragments are given by it?

Sticky end fragments
Smooth end fragments
Rough end fragments
Blunt end fragments
Gum end fragments

Which of the following is the correct explanation of Gibson assembly?

Joining DNA fragment with RNA fragment by ligase
DNA fragments combining by restriction enzyme/ligase cloning
Binding RNA fragments
Plasmid assembly
Joining rRNA with mRNA

Which important parts are present in pBR322?

Penicillin--Ori--EcoRI; HindIII, EcoRV, BamHI
Lipramycin--EcoRI; HindIII, EcoRV, BamHI
Cephalosporin--Ori--EcoRI; HindIII, EcoRV, BamHI
amp--tet--Ori--EcoRI; HindIII, EcoRV, BamHI
Ampicillin--terramycin--Ori--EcoRI; HindIII, EcoRV, BamHI

What is the main purpose of rDNA technology?

DNA cloning
DNA-RNA hybrid cloning
RNA cloning
To study transformation
To study gene mutation

Which DNA is developed from RNA precursor?

rDNA
mDNA
Naked DNA
ssDNA
cDNA

Which of the following is called hybridisation probe?

Any heavy ds/ss DNA fragment
Any heavy ds/ss RNA fragment
Radioactively labelled DNA/RNA fragment
Heavy element labelled lipid fragment
Heavy isotope labelled protein

Where is rDNA multiplied?

In target vector
In cloning vector
In viruses
In archaebacteria
In eukaryotic protista

Which chemical is used for transformation of rDNA into cloning vector?

Calcium phosphate
Calcium chloride
Sodium chloride
Sodium alginate
Glycol

Which of the following colour marker chemicals is mixed to the medium to identify rDNA cloned vectors?

x-gal
Calcium alginate
Thymol blue
Safranine
Phenophthalene

Which of the following correctly describes rDNA?

Crossover DNA
Mutated DNA
Hybrid DNA
Transcripted DNA
Plasmid DNA

Which of the following is the correct use of thermocycler?

To heat the bacterial medium
For sterile inoculation
Amplification of DNA fragment
For autoclaving
For hybridisation

Which characters should be possessed by a plasmid for rDNA technology?

Genes related to ori-restriction site
Genes of ori - insulin gene - restriction site
Colicin gene - ori - restriction site
Cloning gene - colicin gene - ori
Ori - colicin gene - insulin gene

Restriction endonucleases break dsDNA at ______ in _______ direction.

episome, 5'-3'
episome, 3'-5'
palindrome, 5'-3'
palindrome, 3'-5'
perisome, 5'-3'

Which type of endonucleases are generally used in genetic engineering?

Type-I
Type-II
Type-III
Type-IV
Type-V

Which is the recognition site of EcoR I?

5'--GAT ATC--3'
5'--- CCWGG--3'
5'--G AATTC--3'
5'--G GATCC--3'
5'----A AGCTT---3'

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