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Microbial Metabolism (GATE)

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Which of the following vitamins acts as coenzyme in the synthesis of purines and pyrimidines?

  1. Biotin

  2. Pantothenic acid

  3. Folic acid

  4. Vitamin E

  5. Vitamin K


Correct Option: C
Explanation:

Folic acid is a coenzyme used in the synthesis of purines and pyrimidines.

Which of the following pairs is/are not correctly matched?

  1. Gallionella - Iron bacteria
  2. Alcaligenes - Carboxydobacteria
  3. Rhodospirillum - Hydrogen bacteria
  4. Nitrobacter - Nitrifying bacteria
  1. 1 and 2

  2. 1 and 4

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: B
Explanation:

Gallionella is an iron bacterium which oxidises Fe++ as a source of energy for lithoautotrophic growth. Nitrobacter is a nitryfying bacterium which oxidises NO2 to NO3.

Ethanol is not one of the fermentation end products of

  1. Enterobacter

  2. Clostridium

  3. Saccharomyces

  4. Escherichia

  5. Salmonella


Correct Option: B
Explanation:

Clostridium yields butanol, acetone, isopropyl alcohol and CO2 as fermentation end products.

Which of the following matchings is/are correct?

  1. Cyanobacteria : Chlorosomes
  2. Green sulphur bacteria : Thylakoids
  3. Purple bacteria : Chromatophores
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: C
Explanation:

Sites of photosynthesis in cyanobacteria, green sulphur bacteria and purple bacteria are thylakoids, chlorosomes and chromatophores, respectively.

Match the columns:

 
Column - I Column - II
P. Thiamine 1. Coenzyme in flavoproteins
Q. Riboflavin 2. Part of coenzyme cocarboxylase
R. Pyridoxine 3. Coenzyme involved in the transfer of methyl groups
S. Cyanocobalamin 4. Coenzyme in amino acid metabolism
  1. P - 1; Q - 2; R - 3; S - 4

  2. P - 1; Q - 3; R - 2; S - 4

  3. P - 2; Q - 1; R - 3; S - 4

  4. P - 2; Q - 1; R - 4; S - 3

  5. P - 3; Q - 2; R - 4; S - 1


Correct Option: D
Explanation:

Vitamin B1 (Thiamine) is part of coenzyme cocarboxylase. Vitamin B2 (Riboflavin) acts as coenzyme in flavoproteins.

________ yields ethanol, lactic acid, formic acid, butanediol, acetoin, CO2 and H2 as fermentation end products.

  1. Saccharomyces

  2. Clostridium

  3. Escherichia

  4. Enterobacter

  5. Salmonella


Correct Option: D
Explanation:

Enterobacter yields ethanol, lactic acid, formic acid, butanediol, acetoin, CO2 and H2 as fermentation end products.

Which of the following is a pair of microbes yielding lactic acid as fermentation end product?

  1. Propionibacterium and Saccharomyces

  2. Bacillus and Clostridium

  3. Clostridium and Propionibacterium

  4. Saccharomyces and Streptococcus

  5. Bacillus and Streptococcus


Correct Option: E
Explanation:

Both yield lactic acid as fermentation end product.

Which of the following sulphate-reducing bacteria uses organic compounds from pyruvate synthase pathway as major C-source?

  1. Archaeoglobus

  2. Desulfotomaculum

  3. Thermodesulfobacterium

  4. Desulfobacterium

  5. Desulfobacter


Correct Option: C
Explanation:

Thermodesulfobacterium uses organic compounds from pyruvate synthase pathway as major C-source.

Which of the following bacteria yields butanol, acetone, isopropyl alcohol and CO2 as fermentation end products?

  1. Enterobacter

  2. Streptococcus

  3. Salmonella

  4. Clostridium

  5. Saccharomyces


Correct Option: D
Explanation:

Clostridium yields butanol, acetone, isopropyl alcohol and CO2 as fermentation end products.

Identify the false statements.

  1. Ubiquinones are non-protein electron carriers located in the inner mitochondrial membrane.
  2. Entner–Doudoroff pathway occurs both in prokaryotes as well as eukaryotes.
  3. Non-cyclic photophosphorylation is absent in bacterial photosynthesis.
  4. Bacteriochlorophyll absorbs in the range of 250-500 nm.
  1. 1 and 2

  2. 1 and 3

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: D
Explanation:

Both are false statements.Entner–Doudoroff pathway occurs only in prokaryotes.Bacteriochlorophyll absorbs in the range of 670-1040 nm.

Pyruvate acts as metabolic precursor of which of the following amino acids?

  1. Histidine
  2. Serine
  3. Alanine
  4. Valine
  5. Leucine
  1. 1, 2 and 3

  2. 1, 3 and 5

  3. 2, 3 and 4

  4. 2, 4 and 5

  5. 3, 4 and 5


Correct Option: E
Explanation:

Pyruvate acts as metabolic precursor to alanine, valine and leucine.

Identify the FALSE statement(s) regarding non-cyclic phosphorylation?

  1. It is oxygenic process.
  2. Only one light trapping system (PS I) is involved.
  3. No NADPH is synthesised.
  4. NADP acts as last electron acceptor.
  1. 1 and 2

  2. 1 and 3

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: C
Explanation:

Two light trapping systems (PS I and PS II) are involved in non-cyclic phosphorylation.

__________ is an example of exclusively ketogenic amino acids.

  1. Isoleucine

  2. Lysine

  3. Tyrosine

  4. Phenylalanine

  5. Proline


Correct Option: B
Explanation:

A significant amount of metabolic energy can come from amino acid metabolism.

The molecular formula of chlorophyll c1 is

  1. C35H30O5N4Mg

  2. C55H70O6N4Mg

  3. C54H70O6N4Mg

  4. C55H72O5N4Mg

  5. None of these


Correct Option: A
Explanation:

Molecular formula of chlorophyll c1 is C35H30O5N4Mg.

Which of the following is genus of chemosynthetic filamentous sulphur-oxidising bacteria?

  1. Rhodospirillum

  2. Thiobacillus

  3. Chlorobium

  4. Chromatium

  5. Beggiatoa


Correct Option: E
Explanation:

Beggiatoa is a genus of chemosynthetic filamentous sulphur-oxidising bacteria.

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