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Metabolism and Bioenergetics

Description: Gate_BT (4)
Number of Questions: 15
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Tags: Gate_BT (4) Metabolism and Bioenergetics
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Which of the following is an abnormal and hazardous process for biological systems?

  1. Chemosynthesis

  2. Photorespiration

  3. Photo-oxidation

  4. Decolouration


Correct Option: C
Explanation:

Photo-oxidation is the degradation of a polymer surface in the presence of oxygen or ozone. The effect is facilitated by radiant energy, such as UV or artificial light. This process is the most significant factor in weathering of polymers. Proteins, carbohydrates, fats and nucleic acids are all polymers.

DNA also exists as modified structure known as a triple helical DNA. Which of the following does not apply to this modified form of DNA?

  1. It is formed at neutral or acidic pH although being rare.

  2. It is triple-stranded.

  3. It requires Hoogsteen hydrogen bonding only.

  4. It requires Watson-Crick hydrogen bonding.


Correct Option: A
Explanation:

A triple-stranded DNA is a structure of DNA in which three oligonucleotides wind around each other and form a triple helix. One strand binds to a B-form DNA double helix through Hoogsteen or reversed Hoogsteen hydrogen bonds. The triple helix is a partial binding to one of the strands, where the other strand maintains the Watson-Crick pairing. The formation of triple helix is favoured when there are stretches of oligopurines/pyrimidines in a DNA.

Assertion: Glucose is an epimer of mannose. Reason: It differs in configuration at alpha-carbon position.

Which of the following options is correct?

  1. Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

  2. Assertion and Reason both are correct and Reason is not the correct explanation of Assertion.

  3. Assertion is correct but Reason is wrong.

  4. Assertion and Reason both are incorrect.


Correct Option: C
Explanation:

Epimers are the structures that differ only at one position in their configuration. Glucose and mannose are epimers but they differ at 2' C position.

The effects of pH on enzyme-catalysed reaction include which of the following? A. The direction of the reaction may be influenced by the [H+]. B. The ionisation state of enzymes dissociating groups may be modified. C. The ionisation state of substrate may be modified. D. The protein may be denatured at certain pH values.

  1. Only A and B

  2. Only A and C

  3. Only A, B and C

  4. All A, B, C and D


Correct Option: D
Explanation:

The pH of the medium will have its effect on a enzyme-catalysed reaction in one or the other form. The options B and C define the action of protons on the dissociating groups and substrate. Availability of H+ will enhance the ionising ability and therefore, will affect the direction of reaction. It is obvious for protein to get partially denatured during the process when pH fluctuates.

Directions: Read the following information and answer the question given below: Proteins acquire specific and precise folds to perform a desired function. Therefore, the foldings of the proteins are highly essential and specific. Folds are further composed of two major 2o structures, i.e. alpha helices and beta sheets.

What is the helical rise (pitch) for a normal alpha-helix?

  1. 5.4 Ao

  2. 3.6 Ao

  3. 4.0 Ao

  4. 5.1 Ao


Correct Option: A
Explanation:

The pitch of the alpha-helix is 5.4 Å or 0.54 nm, which is equal to 1.5 (translation height) * 3.6 (residues in one turn). Therefore, the length of 153 amino acid protein will be 153/.54 = 283 nm.

Directions: Read the following information and answer the question given below: Proteins acquire specific and precise folds to perform a desired function. Therefore, the foldings of the proteins are highly essential and specific. Folds are further composed of two major 2o structures, i.e. alpha helices and beta sheets.

If a protein is a 153 residues protein and given that its structure is a continuous alpha-helix, what would be the length of the protein?

  1. 552 nm

  2. 360 nm

  3. 534 nm

  4. 283 nm


Correct Option: D
Explanation:

The pitch of the alpha-helix is 5.4 Å or 0.54 nm, which is equal to 1.5 (translation height) * 3.6 (residues in one turn). Therefore, the length of 153 amino acid protein will be 153/.54 = 283 nm

Based on the structural insights of the following compounds, mark the one that does not fit in the group of amphipathic compounds.

  1. Oleic acid

  2. Phosphatidylcholine

  3. Cholesterol

  4. Succinate


Correct Option: D
Explanation:

Oleic acid and cholesterol are uncharged species but have components, which are hydrophobic and others which are hydrophillic. Phosphatidylcholine has a +ve charge on N and a -ve on P, which makes it an amphipathic compound.

Proteins are the building blocks of life. All the major processes in body are regulated by one or the other protein. Therefore, it becomes very vital to assess proteins in a professional manner, for which many sophisticated techniques and protocols are available. The standard protein assessment protocol includes: Purification, Digestion, Sequencing, Assays. Based on the provided information and knowledge of protein assessment methods, answer the following question:

Which of the following would you use before the sequencing of a peptide?

  1. Dansylation

  2. Microarray protein expression

  3. Enzymatic fragmentation

  4. Fast Protein Liquid Chromatography


Correct Option: C
Explanation:

Before sequencing of peptides using mass spectrometry and after purification of peptides from the mixture, peptides need to be cleaved by different enzymes in order to have standardized sequencing. 

Which of the following organelles would you select, if the given statement is considered to be an absolute fact? “More the levels of intracellular IP3 (inositol Triphosphate), more is the efflux of Ca++ from its membranes in the cytoplasm.”

  1. Lysosomes

  2. Mitochondria

  3. Peroxisomes

  4. Endoplasmic reticulum


Correct Option: D
Explanation:

The Ca++ channel in the membrane of endoplasmic reticulum is also an IP3 receptor and therefore, an increase in IP3 will enhance the activity of the channel, resulting in increased efflux of ions.

Amongst chlorophyll-a (C55H72O5N4Mg) and chlorophyll-b (C55H70O6N4Mg), which one has –CHO group and which has –CH3 group at C-3 position, respectively?

  1. CHO in Chl-a and CH3 in Chl-b

  2. Chl-a has both CHO and CH3 groups

  3. CH3 in Chl-a and CHO in Chl-b

  4. No CHO in Chl-b and CH3 in Chl-a


Correct Option: C
Explanation:

Both the chlorophylls have similar structures but they do differ in their compositions, which cause the adaptability in different scenarios. From the given formula, it can be estimated that Chl-a has two more H and an O less than Chl-b. Therefore, CHO is present in Chl-b.

Proteins are the building blocks of life. All the major processes in body are regulated by one or the other protein. Therefore, it becomes very vital to assess proteins in a professional manner, for which many sophisticated techniques and protocols are available. The standard protein assessment protocol includes: Purification, Digestion, and Sequencing. Based on the provided information and knowledge of protein assessment methods, answer the following question:

Which of the following is not a method for protein purification?

  1. High-performance liquid chromatography (HPLC)

  2. Mass spectrometry

  3. Fast protein liquid chromatography (FPLC)

  4. Alcohol precipitation


Correct Option: B
Explanation:

Amongst the above, mass spectrometery is used to sequence or analyse the peptide sequence, while all others are used to purify peptides or proteins.

Which of the given options will consolidate the meaning of the following characteristics of biological systems? A. Spontaneous folding of proteins. B. Formation of precise groups by birds or animals like herds, flocks, fleets, etc. C. Coordinated movement of insects. D. Formation of lipid bilayer membranes and the organisation of lipids.

  1. Entropy in biological systems

  2. Optimisation in biological systems

  3. Self-organisation in biological systems

  4. Patternisation of biological systems


Correct Option: C
Explanation:

The given statements are nothing but examples of self-organisation in biological systems. Self-organisation is a process, where some form of global order or coordination arises out of the local interactions between the components of an initially disordered system.

A water molecule has a highly rigid structure with all bonds being in precise measurement, yet water acquires the shape of the container it is put in. What is the reason behind this?

  1. The water molecules are indeed rigid, but in liquid state, the hydrogen bonding with the neighbouring water molecules is flexible.

  2. The water molecules in liquid state become flexible so as to acquire the shape of the container.

  3. The water molecules have a natural tendency of shape adaptability.

  4. Water’s dielectric constant makes the liquid state very less viscous to allow flexibility.


Correct Option: A
Explanation:

The hydrogen bonding in the water molecules is the cause of flexibility of the liquid and the same is the reason for water being solid in ice state.

Assertion: Carbon is the building block of life. Reason: It is found in greenhouse gases, coal, tar and other poisonous substances like cyanide.

Which of the following options is true?

  1. Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

  2. Assertion and Reason both are correct and Reason is not the correct explanation of Assertion.

  3. Assertion is correct but Reason is wrong.

  4. Reason is correct but Assertion is wrong.


Correct Option: B
Explanation:

Carbon is the most essential element of life as every hydrocarbon that constitutes life on Earth comprises of carbon; be it nucleic Acids, proteins, lipids or carbohydrates. It is considered to be the very first element formed in the tree of life. Indeed carbon is found in greenhouse gases, coal, tar and other poisonous substances like cyanide, but this does not support the fact of carbon being the building block of life.  

Match the given vitamins with their chemical names and the corresponding effect of their deficiency in the human body.

   
VITAMINS CHEMICAL NAME DEFICIENCY
A. Vitamin E I. Niacin 1. Bleeding Diathesis
B. Vitamin B3 II. Biotin 2. Spinocerebellar ataxia
C. Vitamin K III. Cholecalciferol 3. Dermatitis
D. Vitamin D IV. Tocopherols 4. Pellagra
E. Vitamin B7 V. Phylloquinone 5. Rickets
  1. A - IV - 1, B - III - 2, C - II - 3, D - III - 5, E - I - 4

  2. A - IV - 2, B - I - 4, C - V - 1, D - III - 5, E - II - 3

  3. A - IV - 1, B - II - 2, C - V - 3, D - I - 4, E - III - 5

  4. A - IV - 2, B - II - 3, C - III - 1, D - V - 5, E - I - 4


Correct Option: B
Explanation:

Vitamin E is a fat-soluble antioxidant, which prevents production of reactive O during fat synthesis. If this is permitted, the fats will be destroyed that will lead to shedding of myelin sheath: spinocerebellar ataxia. Vitamin D causes rickets due to lack of cholecalciferol. Deficiency of B3(niacin) causes slower metabolic rates and eventually pellagra. Vitamin K, which is found in abundance in green leafy vegetables, is essentially required for post-translational modification. It is known as phylloquinone and its deficiency causes blood related issues.

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