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Prokaryotic and Eukaryotic Cells: Structure and Func...

Description: GATE LS Microbiology Prokaryotic and Eukaryotic Cells Structure and Function
Number of Questions: 20
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Methyl-accepting chemotaxis proteins of bacteria ________.

  1. help to guide the bacterium since they are directly connected to the flagellar motor

  2. can only sense conditions that are favorable for bacterial growth

  3. tell the bacterium which way to go

  4. integrate multiple signals through a two component phospho-relay system


Correct Option: D
Explanation:

Integrate multiple signals through a two component phospho-relay system. The methyl-accepting chemotaxis proteins are present on the outer surface of the bacteria. They are chemical sensors that help the bacteria to sense components present in its surroundings.

It is a hypothesis that mitochondria evolved from endosymbiotic bacteria. Therefore, mitochondria are similar to bacteria in many ways. Which of the following is NOT a similarity between mitochondria and bacteria?

  1. Both have circular, double stranded DNA.

  2. Both have 70S ribosomes.

  3. Both are about the same size.

  4. None of these


Correct Option: B
Explanation:

Both have 70S ribosomes. Ribosomes are cellular organelles that take part in protein synthesis. They are made up of two sub units: small and large. Bacteria have 70S ribosomes. Their small subunit is called 30S and the large one is called 50S. Mitochondria have 55S ribosome: 30S and 40S.

Pleomorphic is a general term used to describe bacteria sometimes. This means ______.

  1. bent rod-shaped

  2. corkscrew-shaped

  3. no particular shape

  4. they are not bacilli or cocci


Correct Option: C
Explanation:

Bacteria do not have a particular shape. Some are long-shaped called bacillus, some are round called cocci, some are spiral called spirillum, etc. Thus, they are called pleomorphic.

Which of the following is FALSE about bacterial flagella?

  1. They are made up of a hollow, rigid protein tube.

  2. They use cytoplasmic ATP as their primary energy source.

  3. They spin either clockwise or anticlockwise.

  4. They are mainly made up of a single protein called flagellin.


Correct Option: B
Explanation:

They use cytoplasmic ATP as their primary energy source. Bacterial flagella help the bacteria to move in its environment. It is driven by a rotary engine present at its base, by proton motive force.

A nuclear pore is a passage between nuclear matrix and cytoplasm. The nuclear pore

  1. allows ribosomes to enter the nucleus

  2. allows DNA to enter the cytoplasm for translation

  3. allows cytoplasmic proteins to enter the nucleus

  4. structurally fortifies the nucleus


Correct Option: C
Explanation:

Allows cytoplasmic proteins to enter the nucleus. In the nucleus, important activities as DNA replication, transcription, etc. take place. These are initiated and mediated by many enzymes that are produced inside the cell. Therefore, the enzymes are transported from cytoplasm to nuclear matrix through nuclear pore.

Flagellar filament of bacteria grows by ________.

  1. assembly of subunits travelling through the hollow flagellum to the tip

  2. assembly of subunits along the length of the filament

  3. elongation from tip of the flagellum by enzymatic addition

  4. elongation from base of the flagellum by enzymatic addition


Correct Option: A
Explanation:

Assembly of subunits travelling through the hollow flagellum to the tip. The flagella of bacterium are made up of protein called flagellin. Flagellum is hollow inside and the flagellin subunits are added through the flagellum to the tip. Flagellum of Bacillus subtilis had been completely sequenced and found to be made up of 304 amino acid residues which have a molecular weight of 32600.

In Gram-negative bacteria, the outer membrane is more permeable than the plasma membrane because _________.

  1. as compared to other membrane phospholipids, LPS is larger

  2. lipoproteins stretch the outer membrane

  3. the lipid bilayer is covered by core polysaccharides

  4. in the outer membrane, porin proteins make holes


Correct Option: D
Explanation:

In the outer membrane, porin proteins make holes. Porin proteins are beta barrel proteins present in the Gram-negative bacteria that allows transport of large sized or charged molecules across the membrane by passive diffusion.

Which of the following describes the F1 particle of mitochondria?

  1. It is a type of ribosome that takes part in synthesis of mitochondrial proteins.

  2. It is present in the mitochondrial inner membrane.

  3. It is a type of storage complex in the mitochondria.

  4. It is made up of enzymes that synthesises ATP.


Correct Option: D
Explanation:

It is made up of enzymes that synthesises ATP. The F1 particle is attached to the inner membrane of mitochondria. It has a motor like structure at the base just as bacterial flagella. The motor rotates and generates ATP.

Which of the following mechanisms is NOT followed by a cell to degrade its own cytosolic proteins?

  1. Phagocytosis and secondary lysosome fusion

  2. Autophagic vacuoles

  3. 26S Proteosome degradation

  4. All of the above


Correct Option: A
Explanation:

Phagocytosis and secondary lysosome fusion. Phagocytosis and lysosome fusion are engulfing of small particles by a cell, generally for its nutrition. These are generally observed in macrophages to destroy bacteria.

Colchicine disrupts the microtubules in a cell. If a eukaryotic cell is given such a treatment, which of the following will happen?

  1. The cell will lose directional movement.

  2. The cell will lose its nutrient transport.

  3. The cell will lose its shape.

  4. All of the above


Correct Option: D
Explanation:

All of the above. Microtubules are infrastructure of a cell and give specific shape to it. Since colchicines disrupt microtubules, thus, shape of the cell will get upset resulting in the above mentioned losses.

In the peptidoglycan cross-linking chain, the third amino acid is L-lysine because

  1. this amino acid is positively charged

  2. this amino acid is hydrophillic

  3. this amino acid has a free amino group for the formation of peptide bond

  4. this amino acid has a large R-side chain


Correct Option: C
Explanation:

This amino acid has a free amino group for the formation of peptide bond. Peptidoglycan of bacterial cell wall is made up of N-acetyl glucosamine (NAG)-N-acetyl muramic acid (NAM). To NAM, a tetra-peptide chain is attached consisting of L-alanine, D-glutamic acid, L-lysine and D-alanine. The L-lysine is attached to a penta-glycine chain sideways, which is further attached to D-alanine of the neighbouring NAG-NAM complex. Since L-lysine needs to form the penta-glycine chain, it should have a free amino group for peptide bond formation. This extensive cross linking gives rigid structure to the peptidoglycan moiety.

The presence of D-amino acids in peptidoglycan layer of bacterial cell wall is because of the fact that

  1. most L-amino acids take part in protein synthesis

  2. D-amino acids fit the structure of the cell wall better than L-amino acids

  3. most peptidases can only cleave L-amino acids

  4. D-amino acids are easier to crosslink in the absence of ribosome


Correct Option: C
Explanation:

Most peptidases can only cleave L-amino acids. Peptidases are enzymes that act on short protein chains into individual amino acids. Many bacteria secrete peptidases that act on L-amino acids and help in their nutrition. But these enzymes cannot degrade their cell walls due to the presence of D-amino acids in their cell walls.

The Act protein secreted by Listeria monocytogenes when it invades a eukaryotic cell

  1. attaches to microtubules, allowing the bacterium to move along the fibres

  2. degrades the cytoskeleton, allowing the bacterium to travel through the plasma membrane to adjacent cells

  3. depolymerises microfilaments, allowing the bacterium to move more easily in the cytoplasm

  4. polymerises actin filaments, causing the bacterium to move through the cytoplasm


Correct Option: D
Explanation:

Polymerises actin filaments, causing the bacterium to move through the cytoplasm. L. monocytogenes invades the cells by polymerising actin filaments in the cytoplasm, because ActA protein is a protease enzyme and specific for actin proteins for its degradation.

The purpose of bacterial endospore is to

  1. help the bacterium to survive in absence of oxygen

  2. help the bacterium to survive in long period of dryness

  3. help the bacterium to grow fast

  4. help the bacterium to spread many seeds in air


Correct Option: B
Explanation:

Help the bacterium to survive in long period of dryness. Endospores are spores that are formed inside a bacterium. Their outer surface is hard which acts as a protective mechanism and allows the bacterium to survive during tough time as in periods of dryness and heat. During the endospore formation, it seems as a non-living thing, but inside it contains live viable bacterium.

During light reaction of photosynthesis, which of the following happens?

  1. Polysaccharides are generated in pyrenoid.

  2. Carbohydrates are synthesised from CO2 and water.

  3. Photons from sunlight are trapped to produce ATP and O2.

  4. All of the above


Correct Option: C
Explanation:

Photons from sunlight are trapped to produce ATP and O2. The light reaction of photosynthesis is also called Hill reaction. This is the first step of photosynthesis where light energy from the sun is absorbed by chlorophyll and hydrolyses H2O to produce O2 and H+ ions. This H+ further reduces NADP to NADPH which finally produces ATP from ADP and Pi. This total step is called photophosphorylation.

Ribosome is the site where mRNA binds and protein is synthesised by the process of translation.

In which of the following comments about 3’ poly(A) tail of mRNA, is the above statement FALSE?

  1. It is not indispensable for protein synthesis.

  2. In the nucleus, it is added to the primary transcript.

  3. It takes part during translation, by aligning eukaryotic mRNA on the ribosome.

  4. It helps to increase the stability and lifespan of mRNA.


Correct Option: C
Explanation:

It takes part during translation, by aligning eukaryotic mRNA on the ribosome. The mRNA is aligned to the ribosome by 7-methylguanosine cap. The 3’ poly(A) tail of mRNA is responsible for its stability and long lifespan.

Ribosome is the site where mRNA binds and protein is synthesised by the process of translation.

According to “Wobble Hypothesis”, which of the following abnormal base pairings might be found?

  1. A - U

  2. G - U

  3. C - I

  4. G - T


Correct Option: B
Explanation:

G - U. According to “Wobble Hypothesis”, the codons of mRNA are degenerate, i.e. there are more codons on the mRNA than the number of amino acids. The codon–anticodon base pairing is relaxed at the third base, which allows G to pair with U, which does not generally happen.

Cell membrane is an indispensable part of cell that maintains vital functions of a cell besides giving proper shape and rigidity to the cell. The intracellular organelles such as vacuoles, lysosomes and golgi bodies are also made up of a similar membrane.

Which of the following substances does not move across the cell membrane of cells by passive diffusion?

  1. Palmitic acid

  2. Aldosterone

  3. Na+

  4. Testosterone


Correct Option: C
Explanation:

 Na+, a charged molecule, crosses membranes through channelby active transport as it is hydrophilic.

Cell membrane is an indispensable part of cell that maintains vital functions of a cell besides giving proper shape and rigidity to the cell. The intracellular organelles such as vacuoles, lysosomes and golgi bodies are also made up of a similar membrane.

When a cell has oxygen limitations, its lysosomes tend to burst and release their contents into the cell. (Consider this statement as true.) As a result of this, what is expected to happen?

  1. The cell undergoes self-digestion and dies.

  2. The cell recycles damaged organelles.

  3. Additional ER is produced in the cell.

  4. Replacement lysosomes are produced by the cell.


Correct Option: A
Explanation:

The cell undergoes self-digestion and dies. Lysosomes contain different types of lytic enzymes. When a lysosome bursts inside a cell, lytic enzymes are released inside the cell resulting into its self-digestion and finally death. This is a usual phenomenon observed by a cell for apoptosis.

Below are certain cell organelles found in prokaryotic as well as eukaryotic cells (Column I) and their associated organelles (Column II). Match them.

 
Column I Column II
A. Intrinsic proteins 1. Cytoplasm
B. Mesosomes 2. Endoplasmic reticulum
C. Bound Ribosomes 3. Invaginated cell membrane
D. Golgi complex 4. Cell membrane
  1. A - 1, B - 2, C - 3, D - 4

  2. A - 1, B - 3, C - 2, D - 4

  3. A - 4, B - 3, C - 2, D - 1

  4. A - 2, B - 1, C - 4, D - 3


Correct Option: C
Explanation:

A - 4, B - 3, C - 2, D - 1: Intrinsic proteins are certain proteins embedded in lipid bilayer of cell membranes. Mesosomes are invaginations in cell membrane of certain gram positive bacteria that are responsible for their respiration. Bound ribosomes are found attached to endoplasmic reticulum in eukaryotic cells. Golgi complexes are dispersed in cytoplasm of eukaryotic cells.

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