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Microbiology (GATE - XL)

Description: GAte
Number of Questions: 15
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Tags: GAte An Overview of Metabolism
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Which of the following sulfate-reducing bacteria uses CO2 from reverse TCA cycle as a major C-Source?

  1. Archaeoglobus

  2. Desulfobacter

  3. Desulfobacterium

  4. Desulfotomaculum

  5. Thermodesulfobacterium


Correct Option: B
Explanation:

Desulfobacter uses CO2 from reverse TCA cycle as a major C-Source.

Which of the following microorganisms has/have least G + C moles%?

  1. Bacillus subtilis

  2. Escherichia coli

  3. Mycobacterium tuberculosis

  4. Spirillum linum

  5. Staphylococcus aureus


Correct Option: D
Explanation:

Among the given, microbes Spirillum linum have least G+C moles % (28-30).

Which of the following enzymes are examples of hydorlases?

  1. Lactate dehydrogenase
  2. Alanine deaminase
  3. Lipase
  4. Sucrase
  5. DNA ligase
  1. 1 and 2

  2. 2 and 3

  3. 3 and 4

  4. 3 and 5

  5. 4 and 5


Correct Option: C
Explanation:

This option is correct because both Lipase and Sucrase are examples of hydrolase.

Assertion (A): During aerobic respiration, lesser number of ATPs is produced in eukaryotes than in prokaryotes. Reason (R): In eukaryotes, some energy is lost when electrons are shuttled across the mitochondrial membranes that separate glycolysis (in cytoplasm) from the electron transport chain.

Directions: In the following question, there are two statements, Assertion (A) and Reason (R). Consider both the statements independently and mark your answers as under:

1. A and R both are correct and R is the correct explanation of A.
2. A and R both are correct and R is not the correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.

  1. A and R both are correct and R is the correct explanation of A.

  2. A and R both are correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R both are incorrect.


Correct Option: A
Explanation:

During aerobic respiration, lesser number of ATPs is produced in eukaryotes (34 molecules of ATP) than in prokaryotes (38 molecules of ATP). This is because of the reason that, In eukaryotes some energy is lost when electrons are shuttled across the mitochondrial membranes that separate glycolysis (in cytoplasm) from the electron transport chain. No such separate mechanism exists in prokaryotes.

Match the column – I (Microorganism) with column – II (Industrial product) and select the correct option.

 
Column – I Column – II
P. Gluconobacter 1. Cheese
Q. Aspergillus 2. Citric Acid
R. Streptococcus 3. Vitamin C
S. Saccharomyces 4. Glycerol
  1. P - 1, Q - 2, R - 3, S - 4

  2. P - 2, Q - 1, R - 4, S - 3

  3. P - 3, Q - 2, R - 1, S - 4

  4. P - 4, Q - 2, R - 3, S - 1

  5. P - 4, Q - 3, R - 2, S - 1


Correct Option: C
Explanation:

Correct answer. Gluconobacter species are used for oxidation of D-sorbitol into L-sorbose in the commercial production of vitamin C (ascorbic acid). Various strains of Aspergillus niger are used in the industrial preparation of citric acid. Streptococcus thermophilus is used in making reduced-fat cheese with similar characteristics to regular full-fat cheese. Glycerol is a major byproduct of ethanol fermentation by Saccharomyces cerevisiae.  

Which of the following is an example of gram-positive bacteria having ability to oxidise glucose by Entner–Doudoroff pathway?

  1. Bacillus subtilis

  2. Enterococcus faecalis

  3. Escherichia coli

  4. Xanthomonas campestris

  5. Zymomonas mobilis


Correct Option: B
Explanation:

Very few gram-positive bacteria have the ability to oxidise glucose by Entner–Doudoroff pathway. Enterococcus faecalis is an example of gram-positive bacteria having the ability to oxidise glucose by Entner–Doudoroff pathway.

Which of the following statements is/are false regarding bacterial photosynthesis?

  1. Bacteriochlorophyll absorbs in the range of 650-750 nm.
  2. Water acts as photosynthetic electron donour.
  3. Non-cyclic photophosphorylation is absent.
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: D
Explanation:

Bacteriochlorophyll absorbs in the range of 800-1000 nm. H2S, other sulfur compounds or certain organic compounds act as photosynthetic electron donour in bacterial photosynthesis.

Entner-Doudoroff pathway was first discovered by Michael Doudoroff and Nathan Entner in

  1. Azotobacter beijerinckii

  2. Enterococcus faecalis

  3. Pseudomonas saccharophila

  4. Rhizobium japonicum

  5. Zymomonas mobilis


Correct Option: C
Explanation:

Entner-Doudoroff pathway was first reported by Michael Doudoroff and Nathan Entner in Pseudomonas saccharophila.

Which of the following statements is correct about Aerotolerant anaerobes?

  1. They are completely dependent on atmospheric oxygen for growth.

  2. They are able to grow under anaerobic conditions, but develop most rapidly in an aerobic environment.

  3. They grow equally well in the presence or absence of oxygen.

  4. It does not tolerate oxygen and dies in its presence.

  5. They thrive in an environment low in oxygen.


Correct Option: C
Explanation:

Aerotolerant anaerobes grow equally well in presence or absence of oxygen.

Which of the following is/are non-protein electrons carrier molecules in electron transport chains?

  1. Flavoproteins
  2. Cytochromes
  3. Ubiquinones
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: C
Explanation:

Ubiquinones (also known as Coenzyme Q) are non-protein electron carriers located in the inner mitochondrial membrane.

Which of the following is a genus of microaerophilic bacteria?

  1. Campylobacter

  2. Bacteroides

  3. Clostridium

  4. Bacillus

  5. Escherichia


Correct Option: A
Explanation:

Campylobacter is a genus of bacteria that are gram-negative, spiral, and microaerophilic. Microaerophiles require oxygen levels in the range of 2 to 10% for their growth and are damaged by the normal atmospheric level of oxygen (20%).

Which of the following plants would be expected to grow at refrigeration temperatures?

  1. Psychrophiles

  2. Psychrotrophs

  3. Mesophiles

  4. Thermophiles

  5. Hyperthermophiles


Correct Option: A
Explanation:

Psychrophiles would be expected to grow at refrigeration temperatures. Psychrophiles grow at 0°C and has an optimum growth temperature of 15°C or lower.

Which of the following statements is/are true regarding Entner–Doudoroff pathway?

  1. It is an alternate series of reactions that catabolise glucose to pyruvate.
  2. Occurs both in prokaryotes as well as eukaryotes.
  3. Has a net yield of 2 ATP for every glucose molecule metabolised.
  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3


Correct Option: A
Explanation:

Entner–Doudoroff pathway is an alternate series of reactions that catabolise glucose to pyruvate using a set of enzymes different from those used in glycolysis or pentose phosphate pathway.

Which of the following is a sulphate reducing bacterium?

  1. Gallionella ferruginea

  2. Desulfotomaculum carboxydivorans

  3. Rhodospirillum rubrum

  4. Beijerinckia derxii

  5. Streptococcus thermophilus


Correct Option: B
Explanation:

Desulfotomaculum carboxydivorans is a moderately thermophilic, anaerobic, chemolithoheterotrophic, sulfate-reducing bacterium.

Match the column – I (Antibiotic) with column – II (Source) and select the correct option.

 
Column - I Column - II
A. Actinomycin 1. Streptomyces antibioticus
B. Chloromycin 2. Streptomyces venezuelae
C.Leucomycin 3. Streptomyces kanamyceticus
D. Tetramycin 4. Streptomyces rimosus
  1. A - 1, B - 3, C - 2, D - 4

  2. A - 3, B - 1, C - 4, D - 2

  3. A - 2, B - 1, C - 3, D - 4

  4. A - 1, B - 2, C - 4, D - 3

  5. A - 1, B - 2, C - 3, D - 4


Correct Option: E
Explanation:

Actinomycin is obtained from Streptomyces antibioticus. Chloromycin is obtained from Streptomyces venezuelae. Leucomycin is obtained from Streptomyces kanamyceticus. Tetramycin is obtained from Streptomyces rimosus.

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