Molecular Biology
Description: GATE: Biotechnology: Molecular Biology & Genetics | |
Number of Questions: 20 | |
Created by: Jayanti Mahajan | |
Tags: Genetics Molecular Biology and Genetics |
In an eukaryote during the transcription holoenzyme, RNA polymerase binds to a DNA sequence and the DNA assumes to have a saddle like structure at that point. What is that region on the DNA called?
Which of the following statements is true in case of RNA polymerase III enzymes?
E.coli bacteria are placed in a medium, which is inoculated with radioactive guanosine for 5 minutes. Then it was made to replicate in a normal medium. Which of the following is correct for the replication process of DNA?
Wobble hypothesis was proposed by F.H.C.Crick (1966) from the structure of anticodon loop in tRNA. Which one of the following is established by this hypothesis?
With which of the following does the operator gene in an operon of a prokaryote (E. coli) combine?
Translation of proteins from mRNA is characterised by formation of specific structures during the process. Which one is correct for polysome formation?
Which of the following is/are responsible for the prevention of ribosomal association at the end of initiation step?
Different antibiotics affect translation in various ways. Some of them prevent ribosomal association with mRNA or block amino acid binding sites on the ribosome to cause damage in the process of translation.
What would happen if in a gene encoding a polypeptide of 100 amino acids, the 83rd codon (UAC) is mutated to UAG?
Splicing is a post transcriptional modification in which newly transcribed hetero nuclear RNA is modified into mRNA. Faulty splicing can cause diseases in any organism. Identify the way by which faulty splicing can cause thalasemia in humans.
How does chloramphenicol affect bacterial translation?
Different antibiotics affect translation in various ways. Some of them prevent ribosomal association with mRNA or block amino acid binding sites on the ribosome to cause damage in the process of translation.
Proteins involved in Hsp70 system are ___________.
Chaperons are heat shock proteins, which facilitate the interactions on the polypeptide surface to finally give the specifc conformation of proteins. They are categorised into two major groups; Hsp70 system and chaperonine system.
Which of the following is a short lived RNA having molecular weight ranging from 500000 to 1000000?
On the basis of life span, molecular weight, function, etc. RNAs are classified into various types like mRNA, rRNA, tRNA smRNA, scRNA, etc.
Match the following:
Column A | Column B |
P. Translation | i. Production of ribosomes |
Q. Nucleolus | ii. Ribosomes |
R. Crick | iii. DNA polymerase |
S. Replication | iv. Central dogma |
Proof reading and DNA repair are the characteristic functions of DNA polymerase I in prokaryotic replication. Identify the enzyme having the same function in eukaryotes.
In eukaryotic replication, 5 different kinds of polymerases have been demonstrated. They perform different functions.
Match the following: Column A Column B P. PCNA i. Single strand binding protein Q. RPA ii. Prevent continuous DNA loss at the end of Chromosome R. Telomeres iii. Fragmented DNA produced on the lagging strand S. Okazaki fragments iv. Leading strand synthesis
If a sudden mutation occurs to the gene responsible for coding DNA polymerase α in a unicellular organism, which function will be stopped in that cell?
In eukaryotic replication, 5 different kinds of polymerases have been demonstrated. They perform different functions.
“Inhibition of the prokaryotic transcription is caused by rifampin, an antibiotic.” Which of the following options is correct for rifampin action?
Match the following: Column A Column B P. Repressor protein i. Fine structure of gene of R II locus in T4 phage Q. Puromycin ii. Produced from inducer R. eEF1βγ iii. Premature termination of polypeptide S. Benzer iv. Eukaryotic translation
Who brings about the mRNA and rRNA processing?
On the basis of life span, molecular weight, function, etc. RNAs are classified into various types like mRNA, rRNA, tRNA smRNA, scRNA, etc.
Which of the following statements is not true for chaperonin?
Chaperons are heat shock proteins, which facilitate the interactions on the polypeptide surface to finally give the specifc conformation of proteins. They are categorised into two major groups; Hsp70 system and chaperonine system.