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Biotechnology

Description: Biotechnology Antibiotics and Pigments etcBiochemistryMicrobiologyMolecular Biology and GeneticsBiotechnology
Number of Questions: 15
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Tags: Biotechnology Antibiotics and Pigments etc Biochemistry Microbiology Molecular Biology and Genetics
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Microbes used as single-cell proteins (SCPs) are:

  1. Chlorella, Spirulina, Yeasts

  2. Propionibacterium, Jatropha, Hevea brasiliensis

  3. Agrobacterium tumifacium, Spirulina, Hevea brasiliensis

  4. Propionibacterium, Agrobacterium tumifacium, Yeasts

  5. Chlorella, Spirulina, Agrobacterium tumifacium


Correct Option: A
Explanation:

All are used as single-cell proteins.

The actinobacteria of the genus Streptomyces are of immense importance to both ecological and human welfare. Which of the following antibiotics are derived from the members of genus Streptomyces?

  1. Penicillin, Streptomycin, Griseofulvin

  2. Chloramphenicol, Griseofulvin, Oxytetracycline

  3. Streptomycin, Chloramphenicol, Oxytetracycline

  4. Streptomycin, Oxytetracycline, Bacitracin

  5. Griseofulvin, Chloramphenicol, Penicillin


Correct Option: C
Explanation:

Streptomycin: Streptomyces griseus Chloramphenicol: Streptomyces venezuelae Oxytetracycline: Streptomyces rimosus All are derived from members of genus Streptomyces.

Which of the following is a pair of starch-debranching enzymes?

  1. Glucoamylase and pullulanase

  2. Glucoamylase and amylase

  3. Pullulanase and isoamylase

  4. Isoamylase and amylase

  5. Glucose isomerase and amylase


Correct Option: C
Explanation:

Both are starch-debranching enzymes.

Which of the following statements is true regarding enzymes?

  1. Induced fit theory of enzymes was given by Emil Fischer.

  2. Michaelis constant is the substrate concentration at which enzyme reaction proceeds at half the maximum velocity.

  3. Synthesis of enzymes is directed by tRNA.

  4. Enzymes exists in cells as solids.

  5. Zymogen is an enzyme.


Correct Option: B
Explanation:

Michaelis constant is the substrate concentration at which the reaction velocity is 50% of the Vmax.

Clones can be obtained by:

  1. tissue culture, micro-propagation and asexual reproduction

  2. tissue culture, micro-propagation and somatic hybridization

  3. transplantation, micro-propagation and somatic hybridization

  4. recombinant DNA technology, tissue culture and somatic hybridization

  5. recombinant DNA technology, asexual reproduction and transplantation


Correct Option: A
Explanation:

Clones can be obtained by all the three methods.

Mendel studied seven contrasting characters for his breeding experiment with Pisum sativum. Which of the following characters did he identify as dominant characters?

  1. Yellow colour of seeds, white colour of flower, inflated seed pods

  2. Green colour of unripe pods, axillary position of flowers, round shape of seeds

  3. Tall stem, constricted seed pods, terminal position of flowers

  4. Green colour of seeds, purple colour of flower, inflated seed pods

  5. Yellow colour of unripe pods, axillary position of flowers, round shape of seeds


Correct Option: B
Explanation:

Green colour of unripe pods: Dominant Axillary position of flowers: Dominant Round shape of seeds: Dominant

Which of the following is not correctly matched?

  1. Bordeaux mixture: Fungicide

  2. Sodium chlorate: Herbicide

  3. Zinc sulphide: Rodenticide

  4. Atrazine: Nematicide

  5. DDT: Insecticide


Correct Option: E
Explanation:

DDT is an organochloride insecticide.

Which of the following enzymes is derived from Bacillus licheniformis?

  1. β-glucanase

  2. Pullulanase

  3. Penicillin amidase

  4. Alkaline protease

  5. Neutral protease


Correct Option: D
Explanation:

Alkaline protease is derived from Bacillus licheniformis.

Which of the following microbes are used in preparation of yoghurt?

  1. Lactobacillus bulgaricus and Streptococcus thermophilus

  2. Leuconostoc mesenteroids and Enterobacter aerogenes

  3. Enterobactor aerogens and Fusarium moniliforme

  4. Streptococcus lactis and Leuconostoc citrovorus

  5. Fusarium moniliforme and Streptococcus thermophilus


Correct Option: A
Explanation:

Yoghurt is prepared by fermenting artificially concentrated milk with a mixture of Lactobacillus bulgaricus and Streptococcus thermophilus at a temperature of 24-5 oC for 4 hrs followed by fermentation with yeast.

Which of the following sets represents natural insecticides obtained from plants?

  1. Pyrethrum, carbaryl, malathion

  2. Azadirachtin, rotenone, carbaryl

  3. Rotenone, pyrethrum, endosulphan

  4. Carbaryl, endosulphan, lindane

  5. Azadirachtin, rotenone, pyrethrum


Correct Option: E
Explanation:

Azadirachtin:  Natural insect repellent obtained from Azadirachta indica (Neem), Rotenone: A powerful insecticide obtained from roots of Derris elliptica and Lonchocarpus spp., Pyrethrum: A contact insecticide obtained from the plant Chrasanthemum  cinerariaefolium.

Which of the following statements is incorrect?

  1. Insulin is secreted by the by β-cells of islets of Langerhans.

  2. Human insulin is a polypeptide of 51 amino acids arranged in a single chain.

  3. Banting and Best isolated insulin from dog’s pancreas.

  4. Insulin regulates carbohydrate metabolism.

  5. Humulin is synthetic human insulin.


Correct Option: B
Explanation:

Human insulin is a polypeptide of 51 amino acids arranged in two chains (A and B) bearing 21 and 30 amino acids respectively.

Which of the following is a set of organisms with bioherbicide property?

  1. Crotalaria juncea, Trifolium alexandriunum, Medicago sativum

  2. Azolla pinnata, Trifolium alexandriunum, Chrysolina beetles

  3. Metarhizium flavoride, Medicago sativum, Crotalaria juncea

  4. Cactoblastes cactorum, Chrysolina beetles, Metarhizium flavoride

  5. Trifolium alexandriunum, Cactoblastes cactorum, Azolla pinnata


Correct Option: D
Explanation:

Cactoblastes cactorum feeds upon Opuntia, and has controlled the spread of Opuntia in India and Australia. Chrysolina beetles have overcome the problem of Klamath weed (Hypericum perforatum) in USA. Spores of Metarhizium flavoride are used in killing the locusts.

Which of the following matches of ‘Nobel laureates and their work’ is incorrect?

  1. E.B. Lewis, E. Weischaus, C. Nusselein-Volhard : Genetic control of embryonic development

  2. Sydney Brenner, John E. Sulston , H. Robert Horitz : Genetic regulation of organ development and programmed cell death

  3. R. Holly, H.G. Khorana and M.W. Nirenberg : Genetic code and base sequence of t-RNA

  4. A. Korenberg and S. Ochoa : Operon model of gene control

  5. James Watson, Francis Crick and Maurice Wilkins : Double helix structure of DNA


Correct Option: D
Explanation:

The Nobel Prize in Physiology or Medicine 1959 was awarded jointly to Severo Ochoa and Arthur Kornberg for their discovery of the mechanisms in the biological synthesis of RNA and DNA. P.Jacob , J.L. Monod and A.M. Lwoff jointly received Nobel prize in 1965 for operon model of gene control.

Which of the following matches of enzyme and its use is not correct?

  1. Streptokinase: Lysis of blood clots

  2. Rennin: Cheese production

  3. Cellulase: Fruit and vegetable processing

  4. Lipase: Fat and oil processing

  5. Acidic protease: Detergent making


Correct Option: E
Explanation:

Alkali proteases find extensive application in detergent and leather industries. Acidic proteases are used in cheese production.

Which of the following is an incorrectly matched pair?

  1. J. Sumner – Crystallization of first enzyme

  2. F.A. Lipmang – Discovery of coenzyme-A and its role in metabolism

  3. E. Fischer and Krebs - Alteration of enzyme activity by phosphorylation/ dephosphorylation

  4. F. Sanger - Action of enzymes

  5. W. Arber, D. Nathans and Hamilton O. Smith – Discovery of restriction enzymes


Correct Option: D
Explanation:

The Nobel Prize in Physiology or Medicine 1931 was awarded to Otto Warburg for his discovery of the nature and mode of action of the respiratory enzyme. The Nobel Prize in Chemistry 1958 was awarded to Frederick Sanger for his work on the structure of proteins, especially that of insulin.

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