• Microbial Culture and Diseases (GATE - XL)
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Microbial Culture and Diseases (GATE - XL)

Description: GATE
Number of Questions: 15
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Tags: GATE Pure Culture Techniques Role of Microorganisms in Transformation of Organic Matter and in the Causation of Diseases.
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Which of the following is an example of non-selective media which are used in isolation of pathogenic bacteria?

  1. Chocolate blood agar

  2. Hektoen enteric agar

  3. Hoyle's agar

  4. Xylose lysine deoxycholate agar

  5. MacConkey agar

Show answer
Correct Option: A
Explanation:

This option is correct because chocolate blood agar is a non-selective, enriched growth medium used for isolation of pathogenic bacteria.

Which of the following is/are food-borne disease(s)?

  1. Botulism
  2. Kuru
  3. Listeriosis
  4. Consumption

  1. 1 and 2

  2. 1 and 3

  3. 1 and 4

  4. 2 and 3

  5. 2 and 4

Show answer
Correct Option: B
Explanation:

This option is correct because botulism is a food-borne disease caused by Clostridium botulinum.

Which of the following is a sexually transmitted infection caused by bacteria?

  1. Kuru disease

  2. Bovine spongiform encephalopathy

  3. Herpes

  4. Syphilis

  5. Trichomoniasis

Show answer
Correct Option: D
Explanation:

This option is correct because syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum subspecies pallidum. 

Which of the following is a culture medium for a genus of bacteria that colonise the mucosal surfaces of many animals?

  1. Eaton's agar

  2. Thayer-Martin agar

  3. Bismuth sulfite agar

  4. Bordet-Gengou agar

  5. Löwenstein–Jensen medium

Show answer
Correct Option: B
Explanation:

This option is correct because Thayer-Martin agar, a Mueller-Hinton agar with 5% chocolate sheep blood and antibiotics, is used for culturing and primarily isolating pathogenic Neisseria bacteria.

Which of the following is a non-segmented, single-stranded DNA virus?

  1. Rotavirus

  2. Cypovirus

  3. Parvovirus

  4. Rhinovirus

  5. Picornavirus

Show answer
Correct Option: C
Explanation:

Parvoviruses are linear, non-segmented single-stranded DNA viruses.

Which of the following is a pair of catalase-positive bacteria?

  1. Enterobacter and Klebsiella

  2. Enterococcus and Streptococcus

  3. Enterobacter and Enterococcus

  4. Enterococcus and Klebsiella

  5. Klebsiella and Streptococcus

Show answer
Correct Option: B
Explanation:

Enterococcus and Streptococcus are examples of catalase-negative bacteria.

Which of the following gram-positive, soil-dwelling bacteria is used as a biological pesticide?

  1. Beauveria bassiana

  2. Bacillus thuringiensis

  3. Penicillium camemberti

  4. Monascus purpureus

  5. Acremonium chrysogenum

Show answer
Correct Option: B
Explanation:

Bacillus thuringiensis is a gram-positive, soil-dwelling bacterium, commonly used as a biological pesticide.

Which of the following families has/have double-stranded RNA viruses?

  1. Adenoviridae
  2. Rhabdoviridae
  3. Reoviridae

  1. Only 1

  2. Only 2

  3. Only 3

  4. 1 and 2

  5. 2 and 3

Show answer
Correct Option: C
Explanation:

This option is correct.

Which of the following is used to culture or grow certain bacteria, particularly the gram-negative species Legionella pneumophila?

  1. VRBD agar

  2. MSA agar

  3. BCYE agar

  4. PCA agar

  5. Nutrient agar

Show answer
Correct Option: C
Explanation:

This option is correct because buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain bacteria, particularly the gram-negative species Legionella pneumophila.

Match the columns:

 
Column I Column II
P. S. aureus 1. Gram-negative rod-shaped bacteria
Q. L. monocytogenes 2. Gram-negative spiral bacteria
R. B. pertussis 3. Gram-positive coccus-shaped bacteria
S. T. pallidum 4. Gram-positive rod-shaped bacteria

  1. P - 1; Q - 2; R - 3; S - 4

  2. P - 2; Q - 1; R - 4; S - 3

  3. P - 2; Q - 3; R - 4; S - 1

  4. P - 3; Q - 4; R - 1; S - 2

  5. P - 4; Q - 3; R - 2; S - 1

Show answer
Correct Option: D
Explanation:

This option is correct because all are correctly matched.

Which of the following tests is used to detect acetyl methyl carbinol in a bacterial broth culture?

  1. Citrate test

  2. Indole test

  3. Methyl red test

  4. Rapid urease test

  5. Voges-Proskauer test

Show answer
Correct Option: E
Explanation:

This option is correct because Voges–Proskauer test is used to detect acetyl methyl carbinol in bacterial broth culture. 

Which of the following is used for the detection and isolation of gram-negative intestinal pathogens?

  1. Mannitol salt agar

  2. Czapek dox agar

  3. Sabouraud agar

  4. Potato dextrose agar

  5. Eosin methylene blue agar

Show answer
Correct Option: E
Explanation:

This option is correct.

Which of the following diseases is/are caused by bacteria?

  1. Scarlatina
  2. Bronze john
  3. Enteric fever
  4. Desert rheumatism

  1. 1 and 2

  2. 1 and 3

  3. 1 and 4

  4. 2 and 3

  5. 2 and 4

Show answer
Correct Option: B
Explanation:

This option is correct because Scarlatina and Enteric fever are caused by bacteria.

Which of the following diseases is/are caused by positive-sense single-stranded RNA virus?

  1. Hepatitis C
  2. Chikungunya
  3. Rubeola
  4. Infantile paralysis

  1. 1, 2 and 3

  2. 1, 2 and 4

  3. 1, 3 and 4

  4. 2, 3 and 4

  5. 1, 2, 3 and 4

Show answer
Correct Option: B
Explanation:

This option is correct.

Match the following:

 
Column I Column II
P. Edward Jenner 1. Phagocytosis
Q. Elie Metchnikoff 2. Smallpox vaccine
R. Wiener and Landsteiner 3. Hybridomas
S. Milstein, Kohler and Jerne 4. Rh factor

  1. P - 1; Q - 2; R - 3; S - 4

  2. P - 2; Q - 1; R - 4; S - 3

  3. P - 2; Q - 3; R - 4; S - 1

  4. P - 3; Q - 1; R - 4; S - 2

  5. P - 4; Q - 3; R - 2; S - 1

Show answer
Correct Option: B
Explanation:

This option is correct because all are correctly matched.

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