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Plant and Animal Biotechnology

Description: Plant and Animal Biotechnology
Number of Questions: 15
Created by:
Tags: Plant and Animal Biotechnology Cell Suspension Culture Development Biochemistry Biotechnology
Attempted 0/15 Correct 0 Score 0

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

  1. it is an organic solvent

  2. it easily penetrates cells

  3. it protects cells by preventing crystallisation of water

  4. it is also utilised as a nutrient


Correct Option: C

Which one of the following is NOT a protoplast fusion inducing agent?

  1. Inactivated Sendai virus

  2. Ca2+ at alkaline pH

  3. Polyethylene glycol

  4. Colchicine


Correct Option: D

Determine the correctness or otherwise of the following Assertion (a) and Reason (r). Assertion: Plants convert fatty acids into glucose. Reason: Plants have peroxisomes.

  1. both (a) and (r) are true but (r) is not the correct reason for (a).

  2. both (a) and (r) are true and (r) is the correct reason for (a).

  3. (a) is true but (r) is false.

  4. (a) is false but (r) is true.


Correct Option: A

Which one of the following amino acids in proteins does NOT undergo phosphorylation?

  1. Ser

  2. Thr

  3. Pro

  4. Tyr


Correct Option: C

Determine the correctness or otherwise of the following Assertion (a) and Reason (r). Assertion: The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors. Reason: Elicitors induce the expression of enzymes responsible for the biosynthesis of secondary metabolites.

  1. both (a) and (r) are true but (r) is not the correct reason for (a).

  2. both (a) and (r) are true and (r) is the correct reason for (a).

  3. (a) is true but (r) is false.

  4. (a) is false but (r) is true.


Correct Option: C

Apoptosis is characterized by

  1. necrosis

  2. programmed cell death

  3. membrane leaky syndrome

  4. cell cycle arrest process


Correct Option: B

Hypophosphatemia is manifested by an X-linked dominant allele. What proportion of the offsprings from a normal male and an affected heterozygous female will manifest the disease?

  1. ½ sons and ½ daughters

  2. all daughters and no sons

  3. all sons and no daughters

  4. ¼ daughters and ¼ sons


Correct Option: A

In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to P. crown gall disease in plants Q. hairy root disease in plants R. transfer of T-DNA into the plant chromosome S. transfer of Ri-plasmid into the plant cell

  1. S only

  2. P and R only

  3. Q and S only

  4. Q only


Correct Option: B

Embryonic stem cells are derived from

  1. fertilized embryo

  2. unfertilized embryo

  3. sperm

  4. kidney


Correct Option: A

Match the entries in Group I with the enzymes in Group II. Group I| Group II| | P. NAD+| 1. Glutathione peroxidase| | Q. Selenium| 2. Nitrogenase| | R. Pyridoxal phosphate| 3. Lactate dehydrogenase | | S. Molybdenum| 4. Glycogen phosphorylase|

  1. P - 3, Q - 2, R - 4, S - 1

  2. P - 4, Q - 1, R - 3, S - 2

  3. P - 3, Q - 1, R - 4, S - 2

  4. P - 3, Q - 4, R - 2, S - 1


Correct Option: C

The product commercially produced by animal cell culture is

  1. insulin

  2. tissue plasminogen activator

  3. interferon

  4. hepatitis B vaccine


Correct Option: B

HAT (hypoxanthine, aminopterin and thymidine) is used for selecting the hybridomas based on the following I. Only hybridoma will grow since it inherited the HGPRT genes from B-cells and can synthesize DNA from hypoxanthine. II. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterin and due to the lack of HGPRT enzyme.

  1. only I is true

  2. only II is true

  3. both I and II are true

  4. I is true and II is false


Correct Option: C

Match items in Group I with Group II.

 
Group I
Group II
P. Alzheimer's disease
1. H1Ni
Q. Mad cow disease
2. Hemoglobin
R. Sickle cell anaemia
3. Pnons
S. Swine flu
4. Amyloid
  1. P - 4, Q - 3, R - 2, S - 1

  2. P - 3, Q - 4, R - 1, S - 2

  3. P - 2, Q - 1, R - 4, S - 3

  4. P - 1, Q - 2, R - 3, S - 4


Correct Option: A

Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously. Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.

  1. both (a) and (r) are true and (r) is the correct reason for (a)

  2. both (a) and (r) are true but (r) is not the correct reason for (a)

  3. (a) is true but (r) is false

  4. (a) is false but (r) is true


Correct Option: A

Match the herbicides in Group I with the target enzymes in Group II.

 
Group I Group II
P. Glyphosate 1. Nitrilase
Q. Bromoxynil 2. Acetolactatesynthetase
R. Sulphonylureas 3. Dehalogenase
S. Dalapon 4. 5 -Enolpyruvyl shikimate3-phosphate synthase
  1. P - 4, Q - 1, R - 2, S - 3

  2. P - 4, Q - 3, R - 2, S - 1

  3. P - 2, Q - 1, R - 4, S - 3

  4. P - 3, Q - 2, R - 4, S - 1


Correct Option: A
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