• Recombinant DNA Technique (Level : Hard)
Back
0

Recombinant DNA Technique (Level : Hard)

Description: Proteins
Number of Questions: 15
Created by:
Tags: Proteins Recombinant DNA Technology
Start the Quiz
Attempted 0/15 Correct 0 Score 0
‹
›

Arrange the following in the sequence of their usage in recombination DNA technique.

  1. CaCl2
  2. Ligase
  3. EDTA
  4. Restriction endonuclease

  1. 1, 2, 3, 4

  2. 4, 1, 3, 2

  3. 3, 1, 2, 4

  4. 4, 3, 2, 1

Show answer
Correct Option: D
Explanation:

This is the correct sequence. 

Restriction endonucleases are also known as

  1. molecular glue

  2. molecular scissors

  3. molecular markers

  4. reverse transcriptase

Show answer
Correct Option: B
Explanation:

Restriction endonucleases are also known as molecular scissors as they function in cutting the DNA strand.

Which of the following is a palindromic sequence?

  1. 5'- T T G A G T -3'3'- A A C T C A -5'

  2. 5'- G A A T T C -3'3'- C T T A A G -5'

  3. 5'- A A C C A G -3'3'- T T G G T C -5'

  4. 5'- A C T C T G -3'3'- T G A G A C -5'

Show answer
Correct Option: B
Explanation:

It is a palindromic sequence.

Plasmids are autonomously replicating minichromosomes found in

  1. bacteriophages

  2. E. coli

  3. amoeba

  4. paramecium

Show answer
Correct Option: B
Explanation:

E. coli is a bacterium and carries plasmids.

Gel electrophoresis is used to separate

  1. RNA

  2. DNA

  3. protein

  4. All of the above

Show answer
Correct Option: D
Explanation:

Gel electrophoresis is used to separate macromolecules like DNA, RNA and proteins.

According to EFB (European Federation of Biotechnology), biotechnology is the integration of natural science and

  1. organisms

  2. cells

  3. molecular analogs

  4. All of the above

Show answer
Correct Option: D
Explanation:

According to EFB, biotechnology is the integration of natural sciences, organisms, cell parts and molecular analogs for products and services.

Which of the following methods uses a gene gun for genetic engineering?

  1. Vector method

  2. PCR technique

  3. Bolistic method

  4. Plasmid method

Show answer
Correct Option: C
Explanation:

Bolistic method uses a gene gun for genetic engineering. 

In recombinant DNA technology, the purified DNA is precipitated by

  1. distilled water

  2. chilled ethanol

  3. ethanol at 36°C

  4. 70% ethanol

Show answer
Correct Option: B
Explanation:

In recombinant DNA technology, the purified DNA is precipitated by chilled ethanol. 

Who is the father of genetic engineering?

  1. Herbert Boyer

  2. Paul Berg

  3. Rudolf Jaenisch

  4. Jack Williamson

Show answer
Correct Option: B
Explanation:

Paul Berg is known as the father of genetic engineering. 

What is the full form of GMO?

  1. Genetically manipulated organism

  2. Genetically modified organism

  3. Genetically maneuvered organism

  4. Generating mutated organism

Show answer
Correct Option: B
Explanation:

GMO stands for genetically modified organism. 

The first genetically altered primate was

  1. Dolly

  2. Herman

  3. Andi

  4. Polly

Show answer
Correct Option: C
Explanation:

Andi was the first genetically altered primate born in October 2000.

Which of the following techniques uses homologous recombination to target desired changes to a specific endogenous gene?

  1. Genome editing

  2. Gene targeting

  3. Gene trapping

  4. Gene splicing

Show answer
Correct Option: B
Explanation:

Gene targeting is a genetic technique that uses homologous recombination to change an endogenous gene. 

Which of the following is a class of restriction enzymes?

  1. Nucleolase

  2. Exonuclease

  3. Endonuclease

  4. Transcriptase

Show answer
Correct Option: C
Explanation:

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain. 

Which of the following sentences about plasmids is untrue?

  1. Plasmid is always a double-stranded DNA molecule.

  2. Plasmid is always circular.

  3. Plasmid is coated with histone protein.

  4. Plasmid does not carry any vital gene necessary for the cell.

Show answer
Correct Option: C
Explanation:

Plasmid is naked without histone protein.

What is the result of calcium treatment of bacteria?

  1. Bacteria becomes resistant to ampicillin.

  2. Bacteria becomes more permeable to rDNA.

  3. Bacteria gets stained.

  4. Bacterial DNA gets precipitated.

Show answer
Correct Option: B
Explanation:

Bacterial cells become competent by treating them with calcium. 

- Hide questions
ADVERTISEMENT

Find more quizzes from top tags

general knowledge
3615 Quizzes
119858 Questions
 technology
307 Quizzes
36705 Questions
 sports
963 Quizzes
19148 Questions
 history
1187 Quizzes
13475 Questions
 physics
598 Quizzes
13441 Questions
 biology
1160 Quizzes
12174 Questions
 science & technology
551 Quizzes
11015 Questions
 chemistry
490 Quizzes
9892 Questions
 maths
518 Quizzes
9324 Questions
 softskills
0 Quizzes
7131 Questions