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DNA Finger Printing (Level : Easy)

Description: Proteins
Number of Questions: 15
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Tags: Proteins DNA Finger Printing
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Mini-satellites are

  1. 9bp to 80bp sized short sequences of DNA

  2. short coding repetitive regions on the eukaryotic genome

  3. short non-coding repetitive sequences present on chromosomes

  4. region of chromosome after secondary constriction

Show answer
Correct Option: C
Explanation:

Mini-satellites are non-coding repetitive sequences present on the chromosomes.

Who discovered DNA fingerprinting?

  1. Ian Wilmut

  2. Paul Berg

  3. Sir Alec Jeffreys

  4. Herbert Boyer

Show answer
Correct Option: C
Explanation:

Sir Alec Jeffreys discovered DNA fingerprinting in 1984.

Which of the following techniques is used for the separation of DNA fragments in DNA fingerprinting?

  1. Southern blotting

  2. Auto-radiography

  3. Electrophoresis

  4. Density gradient centrifugation

Show answer
Correct Option: C
Explanation:

Electrophoresis is used in DNA fingerprinting for separation of DNA fragments.

Which of the following techniques is used in DNA fingerprinting?

  1. Southern blot

  2. Western blot

  3. Northern blot

  4. FACS

Show answer
Correct Option: A
Explanation:

A southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Thus, it is used for DNA fingerprinting.

What is rif-lip technique used for?

  1. DNA profiling

  2. Genome mapping

  3. Determination of the risk of a disease

  4. All of the above

Show answer
Correct Option: D
Explanation:

Rif-lip technique is used for DNA profiling, genome mapping and determination of the risk of a disease.

When DNA fragments with significantly different base compositions are separated and monitored by absorption spectra of UV-light, the highest peak observed is of

  1. genomic DNA

  2. satellite DNA

  3. proteins associated with DNA

  4. telomereic DNA

Show answer
Correct Option: A
Explanation:

When DNA fragments with significantly different base compositions are separated and monitored by absorption spectra of UV-light, the highest peak observed is of genomic DNA or bulk DNA, which is around 99% of the total DNA.

The DNA fingerprint of a male child is similar to

  1. his father

  2. his mother

  3. 50% to his father and 50% to his mother

  4. no similarity to any of the parents

Show answer
Correct Option: C
Explanation:

The DNA fingerprint of a male child has 50%-50% similarity to both parents.

Which of the following has/have played a major role in DNA fingerprinting?

  1. Telomeres

  2. Satellites

  3. Centromeres

  4. Secondary constriction

Show answer
Correct Option: B
Explanation:

Satellite DNA consists of very large arrays of tandem repeats of non-coding DNA, which are the basis for DNA fingerprinting.

Why is RFLP analysis done?

  1. To know base sequence of DNA fragment

  2. To detect change in the length of the restriction fragments

  3. To amplify smaller DNA segments

  4. To analyse chromosomes on basis of their shape to form a karyotype

Show answer
Correct Option: B
Explanation:

RFLP analysis is done to detect the change in the length of the restriction fragments. 

Which probe is used for the identification of tandem repeats in DNA fingerprinting?

  1. TaqMan-probe

  2. MB probe

  3. VNTR-probe

  4. LNA prime time probe

Show answer
Correct Option: C
Explanation:

VNTR-probe is used for the identification of tandem repeats in DNA fingerprinting.

Which of the following differences form(s) the basis of DNA fingerprinting?

  1. Difference in pattern of genes between individuals

  2. Difference in junk DNA pattern between individuals

  3. Difference in order of genes between individuals

  4. All of the above

Show answer
Correct Option: B
Explanation:

Junk DNA means non-coding DNA, which is present in the satellites. No two individuals have the same pattern of tandem repeats, i.e. junk DNA, thus it forms the basis of DNA fingerprinting.

Which of the following sequences of DNA fingerprinting procedure is correct?

  1. Restriction digestion - DNA isolation - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph

  2. DNA isolation - Electrophoresis - Restriction digestion - Southern blotting - Probe hybridisation - Autoradiograph

  3. DNA isolation - Restriction digestion - Electrophoresis - Probe hybridisation - Southern blotting - Autoradiograph

  4. DNA isolation - Restriction digestion - Electrophoresis - Southern blotting - Probe hybridisation - Autoradiograph

Show answer
Correct Option: D
Explanation:

This is the correct sequence. 

DNA fingerprinting is applicable everywhere except

  1. to settle disputed parentage

  2. criminal identification and forensics

  3. personal identification

  4. amplification of VNTR region

Show answer
Correct Option: D
Explanation:

DNA fingerprinting cannot be applied to the amplification of VNTR region.

A slight difference in the base-pair sequence of the common genes between individuals is known as

  1. dimorphism

  2. polymorphism

  3. mutation

  4. short tandem repeats

Show answer
Correct Option: B
Explanation:

Polymorphism refers to a slight difference in the base-pair sequence of the common genes between individuals.

Phenotypic appearance of each individual is unique because

  1. entire DNA of all individuals is different

  2. repetitive sequences of each individual are unique

  3. telomeres of each individual are unique

  4. size of chromosomes of each individual is unique

Show answer
Correct Option: B
Explanation:

Repetitive sequences of each individual are unique.

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