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Food Production and Breeding (Class - XII)

Description: Tissue culture Tissue CultureTissue CulturePlant Breeding
Number of Questions: 15
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Tags: Tissue culture Tissue Culture Plant Breeding
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Who first isolated a single plant cell for culturing?

  1. Muir

  2. Steward and Shaanz

  3. Haberlandt

  4. Gautheret

Show answer
Correct Option: C
Explanation:

Haberlandt was the first scientist who isolated a single plant cell and cultured it over artificial culture media.

What is/are the source(s) of protoplast in protoplast culture technique?

  1. Sepals

  2. Leaves

  3. Buds

  4. Root meristem

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Correct Option: B
Explanation:

Protoplast is obtained from leaves of a healthy plant.

What is invoked in paper raft technique?

  1. An organ

  2. Protoplast

  3. Single cell

  4. Callus

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Correct Option: C
Explanation:

Single cell culturing is used in paper raft technique.

Which type of tissue culture uses unorganised and undifferentiated mass of tissue?

  1. Organ culture

  2. Callus culture

  3. Protoplast culture

  4. Micropropagation

Show answer
Correct Option: B
Explanation:

Unorganised and undifferentiated mass of tissue is used in callus culture.

Homokaryons are produced in

  1. induced fusion

  2. nucellus culture

  3. spontaneous fusion

  4. cell suspension technique

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Correct Option: C
Explanation:

Homokaryons are produced by fusion of protoplasts lying in proximity.

Which tissue culture is used in horticulture to produce haploid plants?

  1. Apical meristem tissue culture

  2. Ovary tissue culture

  3. Nucellar tissue culture

  4. Pollen culture

Show answer
Correct Option: D
Explanation:

Pollen culture is used to produce haploid plants.

Why are clones obtained from nucellar culture better than cutting?

  1. Nucellar tissue helps in formation of tap root system.

  2. Nucellar seedlings are vigorous.

  3. Nucellar seedlings are free from diseases.

  4. All of the above

Show answer
Correct Option: D
Explanation:

Nucellar tissue helps in formation of tap root system. Nucellar seedlings are free from diseases. Nucellar seedlings are vigorous.

Which criterion is used for virtual selection of hybrids?

  1. Height of plant

  2. Products secreted

  3. Pigmentation

  4. Size of nucleus

Show answer
Correct Option: C
Explanation:

Pigmentation of the parental protoplasts is used for identification of hybrid cells under microscope.

Cybrids are formed due to

  1. fusion of nuclei

  2. fusion of cytoplasms

  3. fusion of plastids

  4. fusion of mitochondria

Show answer
Correct Option: B
Explanation:

Cybrids are formed by fusion of cytoplasms only.

Which plant tissue culture technique is used to improve quality of fruits?

  1. Embryo culture

  2. Ovary culture

  3. Pollen culture

  4. Apical meristem culture

Show answer
Correct Option: B
Explanation:

Ovary culture is used to improve quality of fruits.

What is the removal of stamens from flower during hybridisation called?

  1. Castration

  2. Self fertilisation

  3. Emasculation

  4. Sterilisation

Show answer
Correct Option: C
Explanation:

The removal of stamens from flower during hybridisation is called emasculation.

Which of the following is/are suitable osmotic stabilising agent(s) used for obtaining viable and stable protoplast?

  1. Sorbitol

  2. Mannitol

  3. Both (1) and (2)

  4. Pectinase enzyme

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Correct Option: C
Explanation:

Both sorbitol and mannitol are used as stabilising agents for obtaining viable and stable protoplast.

What is the optimum condition required for protoplast regeneration?

  1. 25+/-2 degree centigrade dark

  2. 25+/-2 degree centigrade bright light

  3. 25+/-2 degree centigrade dim light

  4. 35+/-2 degree centigrade dim light

Show answer
Correct Option: C
Explanation:

25+/-2 degree centigrade in dim light is the optimum condition required for protoplast regeneration.

A hybridisation technique which completely eliminates sexual process is

  1. callus culture

  2. organ culture

  3. somatic hybridisation

  4. single cell culture

Show answer
Correct Option: C
Explanation:

Somatic hybridisation produces hybrids by completely eliminating sexual processes.

Which of the following agents is/are used as fusogen(s) in induced fusion?

  1. PEG (poly ethylene glycol)

  2. NaNO3

  3. PVA (poly vinyl alcohol)

  4. All of the above

Show answer
Correct Option: D
Explanation:

PEG, PVA and NaNO3 are used as fusogens.

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