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Biochemistry

Description: Biochemistry Biomolecules and Their ConformationMetabolism (Glycolysis, TCA and Oxidativ e phosphorylation)Chemical and Functional Nature of EnzymesBiochemical and Biophysical Techniques for Macromolecular Analysis
Number of Questions: 20
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Tags: Biochemistry Biomolecules and Their Conformation Metabolism (Glycolysis, TCA and Oxidativ e phosphorylation) Chemical and Functional Nature of Enzymes Biochemical and Biophysical Techniques for Macromolecular Analysis
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Which of the following is a specific chemical test for ketohexoses?

  1. Seliwanoff’s test
  2. Molisch test
  3. Biuret test Select the correct answer.
  1. 1 only

  2. 2 only

  3. 3 only

  4. 1 and 2 only

  5. 2 and 3 only


Correct Option: A
Explanation:

Seliwanoff’s test is a specific test for ketohexoses. Concentrated hydrochloric acid dehydrates ketohexoses to form furfural derivatives, which condense with resorcinol to give a cherry red complex.

Which of the following are examples of structural polysaccharides?

  1. Starch
  2. Cellulose
  3. Chitin
  4. Glycogen
  5. Pectin Select the correct answer.
  1. 1 and 2 only

  2. 1, 2, and 3 only

  3. 2, 3 and 4 only

  4. 2, 3, and 5 only

  5. All of the above


Correct Option: D
Explanation:

Cellulose, chitin and pectin: All are structural polysaccharides

Enzyme involved in converting glucose to glycogen is __________.

  1. invertase

  2. glycogen phosphorylase

  3. glucose isomerase

  4. glycogen synthase

  5. glucose oxidase


Correct Option: D
Explanation:

Glycogen synthase is an enzyme involved in converting glucose to glycogen.

Which of the following are examples of chromoprotein?

  1. Haemoglobin and rhodopsin

  2. Zein and keratin

  3. Haemoglobin and casein

  4. Casein and keratin

  5. Rhodopsin and Zein


Correct Option: A
Explanation:

Chromoproteins are proteins, such as hemoglobin or rhodopsin containing a pigmented nonprotein group, as heme, riboflavin, or retinal.

Arabinoxylans are ________.

  1. homopolymers of one type of pentose sugars

  2. homopolymers of one type of hexose sugars

  3. copolymers of two type of pentose sugars

  4. copolymers of two type of hexose sugars

  5. copolymers of pentose and hexose sugars


Correct Option: C
Explanation:

Arabinoxylans are found in both the primary and secondary cell walls of plants and are the copolymers of two pentose sugars: arabinose and xylose.

Enzyme involved in glycolysis, which catalyzes the transfer of a phosphate group from phosphoenolpyruvate to ADP is ________.

  1. pyruvate dehydrogenase

  2. pyruvate kinase

  3. pyruvate carboxylase

  4. glucose 6-phosphatase

  5. phosphofructokinase


Correct Option: B
Explanation:

Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.

A positive Benedict’s test is not given by __________.

  1. glucose

  2. galactose

  3. lactose

  4. maltose

  5. sucrose


Correct Option: E
Explanation:

Sucrose is a non-reducing sugar which does not react with Benedict's reagent.

Which of the following sets represent scleroproteins only?

  1. Albumin, Myoglobin, Thrombin

  2. Collagen, Elastin, Myoglobin

  3. Keratin, Elastin, Myoglobin

  4. Thrombin, Keratin, Elastin

  5. Keratin, Collagen, Elastin


Correct Option: E
Explanation:

Keratin - A fibrous scleroprotein that occurs in the outer layer of the skin and in horny tissues such as hair, feathers, nails, and hooves Collagen - A fibrous scleroprotein in bone and cartilage and tendon and other connective tissue; yields gelatin on boiling Elastin - A fibrous scleroprotein found in elastic tissues such as the walls of arteries.

Enzyme used for clearing blood clots is ____________.

  1. streptokinase

  2. asparaginase

  3. restriction endonuclease

  4. pepsinogen

  5. glutaminase


Correct Option: A
Explanation:

Streptokinase is used to dissolve blood clots that have formed in the blood vessels. 

Most abundant protein in the world is _________.

  1. albumin

  2. collagen

  3. osteocalcin

  4. RuBisCO

  5. keratin


Correct Option: D
Explanation:

RuBisCO (Ribulose-1, 5-bisphosphate carboxylase) is thought to be the most abundant protein in the world, since it is present in every plant that undergoes photosynthesis and molecular synthesis through Calvin cycle.

Which of the following tests is/are used for the detection of cholesterol?

  1. Acrolein test
  2. Salkowski's test

    3.

    Liebermann–Burchard test Select the correct answer.
  1. 1 only

  2. 1 and 2 only

  3. 1 and 3 only

  4. 2 and 3 only

  5. All of the above


Correct Option: D
Explanation:

Salkowski's test is a test for cholesterol. When concentrated sulfuric acid is added to a chloroform solution of cholesterol, the chloroform layer shows red. The Liebermann–Burchard or acetic anhydride test is used for the detection of cholesterol. The formation of a green or green-blue colour after a few minutes is positive.

Which of the following statements is FALSE regarding cholesterol?

  1. The cholesterol molecule is steroid.

  2. Molecular formula of cholesterol is C27H46O.

  3. Cholesterol circulates in blood stream chiefly as free cholesterol.

  4. Cholesterol serves as a precursor for the biosynthesis bile acids.

  5. Cheese is a major dietary source of cholesterol.


Correct Option: C
Explanation:

Cholesterol circulates in blood stream chiefly as low density lipoproteins (LDL) and high density lipoproteins (HDL).

Which of the following is/are molybdoenzyme?

  1. Carbonic anhydrases
  2. Nitrate reductases
  3. Xanthine dehydrogenase Select the correct answer.
  1. 1 only

  2. 1 and 2 only

  3. 1 and 3 only

  4. 2 and 3 only

  5. All of the above


Correct Option: D
Explanation:

Nitrate reductases are molybdenum containing enzymes that reduce nitrate to nitrite. Xanthine dehydrogenase (XDH) is a molybdenum-containing hydroxylase involved in oxidative metabolism of purines. 

Which of the following enzymes are also known as anti-egg white injury factor?

  1. Vitamin B1

  2. Vitamin B3

  3. Vitamin B5

  4. Vitamin B7

  5. Vitamin B9


Correct Option: D
Explanation:

Biotin (Vitamin B7) is also called anti-egg white injury factor because, egg white contains a protein called avidin, which combines with biotin in the intestinal tract and prevents absorption of biotin from intestines.

Which of the following chemical bases is not found in DNA?

  1. 6-aminopurine

  2. 2-oxy-4-amino-pyrimidine

  3. 2, 4-dioxypyrimidine

  4. 2, 4-dioxy-5-methylpyrimidine

  5. 2-amino-6-oxypurine


Correct Option: C
Explanation:

Uracil (2, 4-dioxypyrimidine) is not a part of DNA.

Which of the following chemical bases is not a part of RNA?

  1. 6-aminopurine

  2. 2-oxy-4-amino-pyrimidine

  3. 2, 4-dioxypyrimidine

  4. 2, 4-dioxy-5-methylpyrimidine

  5. 2-amino-6-oxypurine


Correct Option: D
Explanation:

Thymine (2, 4-dioxy-5-methylpyrimidine) is not a part of RNA.

Which of the following is the mismatched pair?

  1. Cheilosis - Riboflavin

  2. Pellagra - Niacin

  3. Scurvy - Ascorbic acid

  4. Pernicious anaemia - Pyridoxine

  5. Beriberi - Thiamine


Correct Option: D
Explanation:

Pernicious anaemia caused when the body does not make enough red blood cells due to lack of cyanocobalamin (vitamin B12). Pyridoxine deficiency (also known as B6 deficiency) is a paediatric disease due to a lack of pyridoxine (or vitamin B6).

Which of the following ‘Vitamin - Chemical Name’ combinations is INCORRECT?

  1. Vitamin B1 - Thiamine

  2. Vitamin B2 - Riboflavin

  3. Vitamin B3 - Niacin

  4. Vitamin B5 - Biotin

  5. Vitamin B9 - Folic acid


Correct Option: D
Explanation:

Chemical name of vitamin B5 is pantothenic acid. Chemical name of vitamin B7 is biotin.

Which of the following is a pair of sulphur containing amino acids?

  1. Leucine and Valine

  2. Cysteine and Methionine

  3. Serine and Threonine

  4. Cysteine and Leucine

  5. Threonine and Methionine


Correct Option: B
Explanation:

Cysteine is a sulphur containing α-amino acid with the chemical formula HOOCCH(NH2)CH2SH. Methionine is sulphur containing α-amino acid with the chemical formula HOOCCH(NH2)CH2CH2SCH3.

Which of the following are examples of heteropolysaccharides?

  1. Dextran
  2. Heparin
  3. Amylopectin
  4. Hyaluronic acid
  5. Chondroitin-4-sulfate Select the correct answer using codes given below:
  1. 1, 2 and 3 only

  2. 1, 3 and 5 only

  3. 2, 3 and 4 only

  4. 2, 4 and 5 only

  5. All of the above


Correct Option: D
Explanation:

Heparin is heteropolysaccharide composed of D-glucuronic acid, L-iduronic acid and N-sulfo-D-glucosamine. Hyaluronic acid is heteropolysaccharide composed of D-glucuronic acid and N-acetyl-D-glucosamine. Chondroitin-4-sulfate heteropolysaccharide composed of D-glucuronic acid and N-acetyl-D-galactosamine-4-O-sulfate.

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