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Genetic Engineering: Manipulating the Molecules of Life - Exploring the Applications of Biotechnology

Description: Genetic Engineering: Manipulating the Molecules of Life - Exploring the Applications of Biotechnology
Number of Questions: 15
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Tags: genetic engineering biotechnology molecular biology dna manipulation
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In genetic engineering, the process of transferring genetic material from one organism to another is known as:

  1. Transformation

  2. Transduction

  3. Conjugation

  4. All of the above


Correct Option: D
Explanation:

Transformation, transduction, and conjugation are all methods used in genetic engineering to transfer genetic material between organisms.

The enzyme used to cut DNA at specific sequences is called:

  1. Polymerase

  2. Ligase

  3. Restriction enzyme

  4. Helicase


Correct Option: C
Explanation:

Restriction enzymes are enzymes that cut DNA at specific sequences, allowing for the manipulation and recombination of genetic material.

The process of inserting a gene into a plasmid or virus for transfer into a host organism is known as:

  1. Cloning

  2. Transfection

  3. Transformation

  4. Gene therapy


Correct Option: A
Explanation:

Cloning involves the insertion of a gene into a plasmid or virus, which is then transferred into a host organism to produce multiple copies of the gene.

The technique used to separate DNA fragments based on their size is called:

  1. Gel electrophoresis

  2. Chromatography

  3. Centrifugation

  4. PCR


Correct Option: A
Explanation:

Gel electrophoresis is a technique used to separate DNA fragments based on their size, allowing for the analysis and identification of specific DNA sequences.

The process of amplifying a specific DNA sequence using a DNA polymerase is known as:

  1. PCR

  2. DNA sequencing

  3. Gene expression

  4. Genetic engineering


Correct Option: A
Explanation:

PCR (Polymerase Chain Reaction) is a technique used to amplify a specific DNA sequence by repeatedly copying it using a DNA polymerase.

The technology that allows for the modification of an organism's genome is called:

  1. Genetic engineering

  2. Gene editing

  3. Recombinant DNA technology

  4. Biotechnology


Correct Option: A
Explanation:

Genetic engineering is the technology that allows for the modification of an organism's genome by introducing or removing specific genes or DNA sequences.

The technique used to determine the sequence of nucleotides in a DNA molecule is called:

  1. DNA sequencing

  2. PCR

  3. Gel electrophoresis

  4. Restriction enzyme digestion


Correct Option: A
Explanation:

DNA sequencing is the technique used to determine the sequence of nucleotides in a DNA molecule, allowing for the identification and analysis of genes and genetic variations.

The process of introducing genetic material into a cell using a virus as a vector is known as:

  1. Transfection

  2. Transformation

  3. Transduction

  4. Gene therapy


Correct Option: A
Explanation:

Transfection is the process of introducing genetic material into a cell using a virus as a vector, allowing for the delivery of genes or therapeutic molecules into cells.

The technology that involves the use of living organisms or their components to develop useful products or processes is called:

  1. Biotechnology

  2. Genetic engineering

  3. Recombinant DNA technology

  4. Molecular biology


Correct Option: A
Explanation:

Biotechnology is the technology that involves the use of living organisms or their components to develop useful products or processes, including genetic engineering, pharmaceuticals, and agricultural applications.

The process of transferring a gene from one organism to another using a bacterium as a vector is known as:

  1. Transduction

  2. Transformation

  3. Conjugation

  4. Gene therapy


Correct Option: A
Explanation:

Transduction is the process of transferring a gene from one organism to another using a bacterium as a vector, allowing for the exchange of genetic material between different organisms.

The technique used to detect the presence of a specific DNA sequence in a sample is called:

  1. PCR

  2. DNA sequencing

  3. Southern blotting

  4. Gel electrophoresis


Correct Option: C
Explanation:

Southern blotting is a technique used to detect the presence of a specific DNA sequence in a sample by transferring DNA fragments from a gel onto a membrane and then hybridizing them with a labeled probe.

The process of transferring genetic material from a donor cell to a recipient cell through direct cell-to-cell contact is known as:

  1. Conjugation

  2. Transformation

  3. Transduction

  4. Gene therapy


Correct Option: A
Explanation:

Conjugation is the process of transferring genetic material from a donor cell to a recipient cell through direct cell-to-cell contact, allowing for the exchange of genetic information between bacteria.

The technology that involves the use of genetically modified organisms (GMOs) to produce useful products or processes is called:

  1. Genetic engineering

  2. Biotechnology

  3. Recombinant DNA technology

  4. Agricultural biotechnology


Correct Option: D
Explanation:

Agricultural biotechnology is the technology that involves the use of genetically modified organisms (GMOs) to produce useful products or processes in agriculture, such as improved crop yields, pest resistance, and enhanced nutritional value.

The technique used to amplify a specific DNA sequence in a sample using a DNA polymerase and a pair of primers is called:

  1. PCR

  2. DNA sequencing

  3. Southern blotting

  4. Gel electrophoresis


Correct Option: A
Explanation:

PCR (Polymerase Chain Reaction) is a technique used to amplify a specific DNA sequence in a sample using a DNA polymerase and a pair of primers, allowing for the production of multiple copies of the target DNA sequence.

The process of introducing genetic material into a cell using a liposome as a vector is known as:

  1. Liposome-mediated transfection

  2. Transformation

  3. Transduction

  4. Gene therapy


Correct Option: A
Explanation:

Liposome-mediated transfection is the process of introducing genetic material into a cell using a liposome as a vector, allowing for the delivery of genes or therapeutic molecules into cells.

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