CRISPR-Cas9 is a versatile and precise gene editing system that allows scientists to make targeted changes to DNA sequences. It has become the preferred technique for gene editing in agriculture due to its efficiency, ease of use, and low cost.

Gene editing in agriculture aims to achieve multiple objectives, including improving crop yield, enhancing nutritional value, increasing resistance to pests and diseases, and creating crops with desirable traits such as drought tolerance or herbicide resistance.

Scientists have successfully used gene editing to create rice plants that are resistant to the brown planthopper, a major pest that causes significant crop losses in Asia. This was achieved by introducing a gene that produces a protein toxic to the pest.

Gene editing can be used to increase the levels of essential vitamins and minerals in crops, reduce the content of antinutrients that interfere with nutrient absorption, and improve the quality of proteins by increasing their digestibility and amino acid composition.

Genome-edited crops are those that have been modified using gene editing techniques, resulting in precise changes to their DNA. They differ from transgenic crops, which are modified by introducing foreign genes from other organisms.

Gene editing offers the advantage of being faster and more precise than traditional breeding methods. It allows scientists to make targeted changes to specific genes, resulting in predictable and heritable traits in crops.

The US Department of Agriculture (USDA) is the primary regulatory body responsible for overseeing the safety and approval of gene-edited crops in the United States.

Opponents of gene editing in agriculture raise concerns about potential risks to human health, such as allergies or toxicity, as well as environmental risks, such as the potential for gene-edited crops to cross-pollinate with wild relatives and impact biodiversity.

The Food and Agriculture Organization (FAO) of the United Nations is responsible for developing guidelines and standards for the safe use of gene editing in agriculture, including risk assessment and management.

Knock-in refers to gene editing techniques that involve inserting or replacing genes in a genome, allowing for the addition of new traits or the modification of existing ones.

Knock-out refers to gene editing techniques that involve disrupting or inactivating a specific gene, resulting in the loss of its function.

Gene activation refers to gene editing techniques that increase the expression of a specific gene, resulting in higher levels of the corresponding protein.

Gene silencing refers to gene editing techniques that reduce or silence the expression of a specific gene, resulting in lower levels of the corresponding protein.

Gene regulation refers to gene editing techniques that involve modifying the regulatory regions of a gene, such as promoters or enhancers, to control its expression.

Gene transfer refers to gene editing techniques that involve introducing a gene from one organism into another, allowing for the transfer of specific traits or characteristics.