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Bioreactor Design and Operation

Description: This quiz is designed to assess your understanding of bioreactor design and operation principles.
Number of Questions: 15
Created by:
Tags: bioreactor design bioreactor operation tissue engineering
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Which type of bioreactor is commonly used for cell culture and tissue engineering applications?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Packed Bed Bioreactor


Correct Option: A
Explanation:

Stirred Tank Bioreactors are widely used in cell culture and tissue engineering due to their ability to provide uniform mixing and aeration, enabling efficient cell growth and proliferation.

What is the primary function of a sparger in a bioreactor?

  1. Temperature Control

  2. pH Control

  3. Gas Exchange

  4. Nutrient Supply


Correct Option: C
Explanation:

Spargers are used to introduce gases, typically oxygen, into the bioreactor to facilitate gas exchange and provide oxygen to the cells.

Which parameter is critical for maintaining cell viability and growth in a bioreactor?

  1. Temperature

  2. pH

  3. Dissolved Oxygen

  4. All of the above


Correct Option: D
Explanation:

Temperature, pH, and dissolved oxygen are all crucial parameters that need to be carefully controlled and maintained within optimal ranges to ensure cell viability and growth in a bioreactor.

What is the purpose of using a scaffold in a bioreactor?

  1. To provide structural support to cells

  2. To enhance cell adhesion and proliferation

  3. To facilitate nutrient and oxygen transport

  4. All of the above


Correct Option: D
Explanation:

Scaffolds serve multiple purposes in a bioreactor, including providing structural support to cells, enhancing cell adhesion and proliferation, and facilitating nutrient and oxygen transport.

Which type of bioreactor is commonly used for large-scale production of biopharmaceuticals?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Fed-Batch Bioreactor


Correct Option: D
Explanation:

Fed-Batch Bioreactors are often used for large-scale production of biopharmaceuticals due to their ability to maintain high cell densities and productivity while allowing for the controlled addition of nutrients and removal of waste products.

What is the main advantage of using a continuous bioreactor over a batch bioreactor?

  1. Higher cell density

  2. Increased productivity

  3. Reduced contamination risk

  4. All of the above


Correct Option: D
Explanation:

Continuous bioreactors offer advantages such as higher cell density, increased productivity, and reduced contamination risk compared to batch bioreactors.

Which type of bioreactor is suitable for culturing shear-sensitive cells?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Wave Bioreactor


Correct Option: D
Explanation:

Wave Bioreactors are designed to provide gentle agitation, making them suitable for culturing shear-sensitive cells.

What is the role of a bioreactor controller in bioreactor operation?

  1. To monitor and adjust process parameters

  2. To maintain sterile conditions

  3. To prevent contamination

  4. To harvest cells and products


Correct Option: A
Explanation:

Bioreactor controllers are used to monitor and adjust process parameters such as temperature, pH, dissolved oxygen, and agitation speed to ensure optimal conditions for cell growth and product formation.

What is the purpose of using a perfusion bioreactor?

  1. To achieve high cell density

  2. To remove waste products

  3. To supply nutrients

  4. All of the above


Correct Option: D
Explanation:

Perfusion bioreactors are designed to achieve high cell density, remove waste products, and supply nutrients continuously, resulting in increased productivity and reduced production time.

Which type of bioreactor is commonly used for the production of monoclonal antibodies?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Fed-Batch Bioreactor


Correct Option: D
Explanation:

Fed-Batch Bioreactors are often used for the production of monoclonal antibodies due to their ability to maintain high cell densities and productivity while allowing for the controlled addition of nutrients and removal of waste products.

What is the primary function of a membrane bioreactor?

  1. Cell separation

  2. Product purification

  3. Gas exchange

  4. Nutrient supply


Correct Option: A
Explanation:

Membrane bioreactors utilize semipermeable membranes to separate cells from the culture medium, enabling cell retention and product purification.

Which type of bioreactor is suitable for culturing cells in a three-dimensional environment?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Spheroid Bioreactor


Correct Option: D
Explanation:

Spheroid Bioreactors are specifically designed to promote the formation and growth of cell spheroids, which can mimic the structure and function of tissues.

What is the main advantage of using a packed bed bioreactor?

  1. High cell density

  2. Reduced shear stress

  3. Improved mass transfer

  4. All of the above


Correct Option: D
Explanation:

Packed Bed Bioreactors offer advantages such as high cell density, reduced shear stress, and improved mass transfer due to the presence of a solid matrix that supports cell growth.

Which type of bioreactor is commonly used for the production of vaccines?

  1. Stirred Tank Bioreactor

  2. Airlift Bioreactor

  3. Hollow Fiber Bioreactor

  4. Fed-Batch Bioreactor


Correct Option: D
Explanation:

Fed-Batch Bioreactors are often used for the production of vaccines due to their ability to maintain high cell densities and productivity while allowing for the controlled addition of nutrients and removal of waste products.

What is the purpose of using a fluidized bed bioreactor?

  1. To achieve high cell density

  2. To improve mass transfer

  3. To reduce shear stress

  4. All of the above


Correct Option: D
Explanation:

Fluidized Bed Bioreactors provide high cell density, improved mass transfer, and reduced shear stress due to the continuous movement of solid particles within the bioreactor.

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