CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats, referring to the unique DNA sequences found in the CRISPR system.

The Cas9 protein acts as a molecular scissors, creating precise double-strand breaks in DNA at targeted locations.

Guide RNA is a short RNA molecule that guides the Cas9 protein to specific DNA sequences, enabling targeted gene editing.

Viral vectors, such as adeno-associated viruses (AAVs), are frequently used in gene therapy due to their ability to efficiently deliver genetic material into target cells.

Gene therapy aims to achieve various objectives, including replacing defective genes, introducing new genes, and correcting genetic mutations, to treat genetic disorders and diseases.

Transgenesis involves the introduction of foreign genetic material into an organism's genome, resulting in the expression of new traits or characteristics.

Gene knockout is a technique used to inactivate or disrupt the function of a specific gene, allowing researchers to study its role in biological processes.

CRISPR-Cas9 is a versatile gene-editing tool that employs RNA-guided nucleases and homology-directed repair to introduce precise changes to DNA sequences.

Gene therapy is primarily used to treat genetic disorders by introducing functional genes to replace defective ones or by correcting genetic mutations.

Transgenesis involves the insertion of a foreign gene into the genome of a host organism, enabling the expression of new traits or characteristics.

TALENs are engineered nucleases that create double-strand breaks in DNA at targeted locations, facilitating gene editing and modification.

ZFNs (Zinc Finger Nucleases) are engineered nucleases that utilize zinc finger proteins to target and modify specific DNA sequences, enabling gene editing and regulation.

Gene therapy employs various mechanisms to treat genetic disorders, including replacing defective genes, introducing new genes, and correcting genetic mutations.

Selective breeding is a traditional genetic engineering technique that involves the breeding of organisms with desired traits to produce offspring with enhanced characteristics.

Genetic engineering in agriculture aims to achieve multiple goals, including developing crops with improved yield and resistance, creating livestock with enhanced productivity, and producing biofuels and renewable energy sources.