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Microfluidics: Manipulating Fluids at the Microscale for Biological Applications

Description: Microfluidics: Manipulating Fluids at the Microscale for Biological Applications
Number of Questions: 14
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Tags: microfluidics fluid manipulation biological applications
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What is the primary advantage of using microfluidics in biological applications?

  1. Increased sample volume

  2. Enhanced sensitivity and precision

  3. Reduced cost of experiments

  4. Simplified experimental setup


Correct Option: B
Explanation:

Microfluidics allows for precise control over fluid flow and manipulation at the microscale, enabling higher sensitivity and accuracy in biological experiments.

Which of the following is NOT a common material used for microfluidic devices?

  1. Polydimethylsiloxane (PDMS)

  2. Glass

  3. Silicon

  4. Paper


Correct Option: D
Explanation:

Paper is not typically used as a material for microfluidic devices due to its porous nature and lack of transparency.

What is the principle behind droplet-based microfluidics?

  1. Generating and manipulating droplets in a continuous fluid stream

  2. Using electric fields to control fluid flow

  3. Creating microchannels with varying cross-sectional areas

  4. Applying pressure gradients to induce fluid flow


Correct Option: A
Explanation:

Droplet-based microfluidics involves the generation and manipulation of discrete droplets within a continuous fluid phase, allowing for precise control over fluid volumes and reactions.

Which technique is commonly used to generate droplets in microfluidic devices?

  1. Flow focusing

  2. Electrospraying

  3. Microchannel emulsification

  4. Inkjet printing


Correct Option: A
Explanation:

Flow focusing is a widely used technique in microfluidics to generate droplets by focusing two immiscible fluids at a specific point, resulting in the formation of droplets with controlled size and uniformity.

What is the purpose of microfluidic mixers?

  1. Mixing different fluids at the microscale

  2. Separating fluids based on their properties

  3. Controlling fluid flow rates

  4. Generating droplets


Correct Option: A
Explanation:

Microfluidic mixers are designed to efficiently mix different fluids at the microscale, enabling rapid and uniform mixing for various applications such as chemical reactions, sample preparation, and cell culture.

Which of the following is NOT a common application of microfluidics in biological research?

  1. Single-cell analysis

  2. Protein crystallization

  3. DNA sequencing

  4. Large-scale manufacturing


Correct Option: D
Explanation:

Microfluidics is primarily used for research and development purposes, and is not typically employed for large-scale manufacturing due to its limited throughput and scalability.

What is the main advantage of using microfluidic devices for single-cell analysis?

  1. Increased sample volume

  2. Higher sensitivity and precision

  3. Reduced cost of experiments

  4. Simplified experimental setup


Correct Option: B
Explanation:

Microfluidic devices enable precise control over fluid flow and manipulation at the single-cell level, allowing for more sensitive and accurate analysis of cellular properties and behaviors.

Which microfluidic technique is commonly used for protein crystallization?

  1. Droplet-based microfluidics

  2. Continuous-flow microfluidics

  3. Microchannel emulsification

  4. Inkjet printing


Correct Option: B
Explanation:

Continuous-flow microfluidics is often employed for protein crystallization, as it provides precise control over mixing conditions and allows for continuous replenishment of reagents, promoting crystal growth and improving crystal quality.

What is the role of microfluidics in DNA sequencing?

  1. Amplifying DNA samples

  2. Separating DNA fragments

  3. Detecting DNA sequences

  4. All of the above


Correct Option: D
Explanation:

Microfluidics plays a crucial role in DNA sequencing by enabling precise manipulation and control of DNA samples, facilitating amplification, separation, and detection of DNA sequences.

Which microfluidic platform is commonly used for cell culture and tissue engineering?

  1. Microfluidic chips

  2. Microfluidic droplets

  3. Microfluidic channels

  4. Microfluidic pumps


Correct Option: A
Explanation:

Microfluidic chips provide a controlled environment for cell culture and tissue engineering, allowing for precise manipulation of culture conditions, cell-cell interactions, and tissue growth.

What is the primary advantage of using microfluidics for drug delivery?

  1. Increased drug dosage

  2. Enhanced drug targeting

  3. Reduced side effects

  4. Simplified drug administration


Correct Option: B
Explanation:

Microfluidics enables precise control over drug delivery, allowing for targeted delivery to specific cells or tissues, reducing systemic side effects and improving therapeutic efficacy.

Which microfluidic technique is commonly used for cell sorting?

  1. Flow cytometry

  2. Dielectrophoresis

  3. Magnetic-activated cell sorting

  4. All of the above


Correct Option: D
Explanation:

Microfluidics offers various techniques for cell sorting, including flow cytometry, dielectrophoresis, and magnetic-activated cell sorting, enabling separation of cells based on size, charge, or magnetic properties.

What is the main challenge associated with scaling up microfluidic devices for commercial applications?

  1. High cost of fabrication

  2. Limited throughput

  3. Difficulty in integrating multiple functions

  4. All of the above


Correct Option: D
Explanation:

Scaling up microfluidic devices for commercial applications poses challenges related to high fabrication costs, limited throughput, and difficulties in integrating multiple functions onto a single device.

Which emerging area of microfluidics research holds great promise for advancing healthcare?

  1. Organ-on-a-chip technology

  2. Microfluidic biosensors

  3. Microfluidic drug delivery systems

  4. All of the above


Correct Option: D
Explanation:

Organ-on-a-chip technology, microfluidic biosensors, and microfluidic drug delivery systems are all promising areas of microfluidics research that have the potential to revolutionize healthcare by providing new tools for drug discovery, disease diagnosis, and personalized medicine.

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