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Gene Editing and Genetic Engineering in Tissue Engineering

Description: This quiz covers the fundamentals of gene editing and genetic engineering techniques used in tissue engineering.
Number of Questions: 15
Created by:
Tags: tissue engineering gene editing genetic engineering
Attempted 0/15 Correct 0 Score 0

Which gene editing technique involves the use of a guide RNA to target and modify specific DNA sequences?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. RNAi


Correct Option: A
Explanation:

CRISPR-Cas9 is a gene editing technique that utilizes a guide RNA to direct the Cas9 protein to a specific DNA sequence, allowing for precise modifications.

What is the primary function of transcription factors in genetic engineering?

  1. DNA replication

  2. Protein synthesis

  3. Gene regulation

  4. Cell division


Correct Option: C
Explanation:

Transcription factors play a crucial role in genetic engineering by regulating gene expression, controlling the production of specific proteins.

Which genetic engineering technique involves the transfer of genetic material from one organism to another?

  1. Gene therapy

  2. Transgenesis

  3. Cloning

  4. Genetic screening


Correct Option: B
Explanation:

Transgenesis is a genetic engineering technique where genetic material is transferred from one organism to another, resulting in the expression of foreign genes in the recipient organism.

What is the main purpose of using stem cells in tissue engineering?

  1. Tissue regeneration

  2. Drug testing

  3. Disease modeling

  4. Genetic analysis


Correct Option: A
Explanation:

Stem cells are utilized in tissue engineering primarily for their ability to differentiate into various cell types, facilitating the regeneration and repair of damaged tissues.

Which gene editing technique employs zinc finger proteins to target and modify specific DNA sequences?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. RNAi


Correct Option: C
Explanation:

Zinc finger nucleases (ZFNs) are gene editing tools that utilize zinc finger proteins to bind to specific DNA sequences and induce targeted modifications.

What is the primary goal of genetic engineering in tissue engineering?

  1. Disease diagnosis

  2. Environmental monitoring

  3. Biofuel production

  4. Modification of genetic material


Correct Option: D
Explanation:

Genetic engineering in tissue engineering aims to modify the genetic material of cells or tissues to improve their functionality, enhance tissue regeneration, or introduce specific therapeutic effects.

Which genetic engineering technique involves the use of viral vectors to deliver genetic material into cells?

  1. Gene therapy

  2. Transgenesis

  3. Cloning

  4. Genetic screening


Correct Option: A
Explanation:

Gene therapy utilizes viral vectors as delivery vehicles to introduce genetic material into cells, aiming to correct genetic defects or provide therapeutic benefits.

What is the primary function of scaffolds in tissue engineering?

  1. Cell proliferation

  2. Gene regulation

  3. Tissue regeneration

  4. Structural support


Correct Option: D
Explanation:

Scaffolds in tissue engineering provide structural support and guidance for cell growth and tissue regeneration, mimicking the extracellular matrix of natural tissues.

Which gene editing technique employs transcription activator-like effector nucleases (TALENs) to target and modify specific DNA sequences?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. RNAi


Correct Option: B
Explanation:

Transcription activator-like effector nucleases (TALENs) are gene editing tools that utilize engineered DNA-binding domains to target and modify specific DNA sequences.

What is the main advantage of using genetic engineering in tissue engineering?

  1. Cost-effectiveness

  2. Simplicity of procedures

  3. Rapid tissue regeneration

  4. Precise control over cellular functions


Correct Option: D
Explanation:

Genetic engineering in tissue engineering allows for precise control over cellular functions, enabling the modification of specific genes or pathways to enhance tissue regeneration or introduce therapeutic effects.

Which genetic engineering technique involves the use of small interfering RNA (siRNA) to silence gene expression?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. RNAi


Correct Option: D
Explanation:

RNA interference (RNAi) utilizes small interfering RNA (siRNA) to target and silence specific gene expression, regulating the production of specific proteins.

What is the primary function of growth factors in tissue engineering?

  1. Cell differentiation

  2. Tissue regeneration

  3. Immune response regulation

  4. Extracellular matrix production


Correct Option: B
Explanation:

Growth factors play a crucial role in tissue engineering by stimulating cell proliferation, differentiation, and tissue regeneration, promoting the formation of new tissues.

Which gene editing technique employs a combination of CRISPR-Cas9 and base editors to introduce precise changes in DNA sequences?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. Base editing


Correct Option: D
Explanation:

Base editing is a gene editing technique that combines CRISPR-Cas9 with engineered enzymes to directly convert one DNA base to another, enabling precise modifications without introducing double-strand breaks.

What is the main challenge associated with using viral vectors in gene therapy?

  1. High cost of production

  2. Limited gene delivery capacity

  3. Immune response activation

  4. Ethical concerns


Correct Option: C
Explanation:

A major challenge associated with using viral vectors in gene therapy is the potential activation of the immune response, which can lead to vector rejection or adverse effects.

Which genetic engineering technique involves the use of gene drives to spread genetic modifications through a population?

  1. CRISPR-Cas9

  2. TALENs

  3. ZFNs

  4. Gene drives


Correct Option: D
Explanation:

Gene drives are genetic engineering techniques that utilize engineered genetic elements to bias inheritance in favor of a specific allele, enabling the spread of genetic modifications through a population.

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