Mounting for Scientific Purposes

Description: Test your knowledge on the techniques and equipment used for mounting specimens for scientific purposes.
Number of Questions: 14
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Tags: mounting scientific microscopy histology preparation
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What is the primary purpose of mounting specimens for scientific purposes?

  1. To preserve the specimen for future study

  2. To facilitate examination under a microscope

  3. To enhance the specimen's aesthetic appeal

  4. To protect the specimen from damage


Correct Option: B
Explanation:

Mounting specimens allows scientists to view them under a microscope, enabling detailed observation and analysis of their structure and features.

Which type of mounting medium is commonly used for histological preparations?

  1. Canada balsam

  2. Glycerin jelly

  3. Polystyrene

  4. Epoxy resin


Correct Option: A
Explanation:

Canada balsam is a traditional mounting medium used in histology due to its refractive index, which closely matches that of glass, making it ideal for microscopy.

What is the function of a coverslip in specimen mounting?

  1. To protect the specimen from dust and contamination

  2. To flatten and secure the specimen on the slide

  3. To enhance the contrast of the specimen

  4. To provide a uniform thickness for observation


Correct Option: B
Explanation:

A coverslip is placed over the mounted specimen to flatten and secure it, ensuring proper adhesion to the slide and preventing movement during observation.

Which mounting technique involves embedding the specimen in a transparent resin?

  1. Whole mount

  2. Slide mount

  3. Section mount

  4. Epoxy embedding


Correct Option: D
Explanation:

Epoxy embedding is a technique where the specimen is embedded in a transparent epoxy resin, allowing for thin sectioning and detailed microscopic examination.

What is the purpose of using a fixative in specimen preparation?

  1. To preserve the specimen's structure and prevent decay

  2. To enhance the specimen's color and contrast

  3. To remove impurities and contaminants from the specimen

  4. To soften the specimen for easier sectioning


Correct Option: A
Explanation:

Fixatives are chemical agents used to preserve the specimen's structure and prevent decay, ensuring that its morphology and cellular components remain intact for further analysis.

Which mounting technique is commonly used for large and delicate specimens?

  1. Whole mount

  2. Slide mount

  3. Section mount

  4. Glycerin jelly mount


Correct Option: A
Explanation:

Whole mount is a technique where the entire specimen is mounted on a slide without sectioning, making it suitable for large and delicate specimens that require minimal manipulation.

What is the primary advantage of using glycerin jelly as a mounting medium?

  1. It provides a high refractive index for improved image clarity

  2. It is water-soluble, allowing for easy removal of the coverslip

  3. It is non-toxic and safe to handle

  4. It prevents the specimen from drying out


Correct Option: B
Explanation:

Glycerin jelly is advantageous because it is water-soluble, making it easy to remove the coverslip if necessary, without damaging the specimen.

Which type of mounting medium is commonly used for temporary preparations?

  1. Canada balsam

  2. Glycerin jelly

  3. Polystyrene

  4. Water-based mounting medium


Correct Option: D
Explanation:

Water-based mounting media are often used for temporary preparations due to their ease of use and removal, allowing for quick observation and subsequent processing of the specimen.

What is the purpose of using a clearing agent in specimen preparation?

  1. To remove pigments and enhance transparency

  2. To fix the specimen's structure and prevent decay

  3. To soften the specimen for easier sectioning

  4. To stain the specimen for better visualization


Correct Option: A
Explanation:

Clearing agents are used to remove pigments and enhance the transparency of the specimen, making it easier to observe under a microscope.

Which mounting technique involves sectioning the specimen into thin slices?

  1. Whole mount

  2. Slide mount

  3. Section mount

  4. Glycerin jelly mount


Correct Option: C
Explanation:

Section mount is a technique where the specimen is sectioned into thin slices, typically using a microtome, and then mounted on a slide for microscopic examination.

What is the primary purpose of using a stain in specimen preparation?

  1. To enhance the specimen's color and contrast

  2. To remove impurities and contaminants from the specimen

  3. To preserve the specimen's structure and prevent decay

  4. To soften the specimen for easier sectioning


Correct Option: A
Explanation:

Stains are used to enhance the specimen's color and contrast, making specific structures and components more visible and distinguishable under a microscope.

Which mounting technique is commonly used for small and delicate specimens?

  1. Whole mount

  2. Slide mount

  3. Section mount

  4. Glycerin jelly mount


Correct Option: B
Explanation:

Slide mount is a technique where the specimen is mounted on a glass slide, typically using a mounting medium, for microscopic examination. This technique is often used for small and delicate specimens that require careful handling.

What is the function of a mounting ring in specimen preparation?

  1. To secure the coverslip to the slide

  2. To provide a uniform thickness for observation

  3. To prevent the specimen from moving during observation

  4. To enhance the contrast of the specimen


Correct Option: A
Explanation:

A mounting ring is used to secure the coverslip to the slide, preventing it from moving or detaching during observation.

Which type of mounting medium is commonly used for permanent preparations?

  1. Canada balsam

  2. Glycerin jelly

  3. Polystyrene

  4. Water-based mounting medium


Correct Option: A
Explanation:

Canada balsam is commonly used for permanent preparations due to its durability and resistance to fading over time, ensuring the longevity of the mounted specimen.

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