Genetic Engineering and Biotechnology

Description: This quiz covers the fundamentals of Genetic Engineering and Biotechnology, including techniques, applications, and ethical considerations.
Number of Questions: 15
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Tags: genetic engineering biotechnology recombinant dna technology gene editing transgenic organisms
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Which technique involves the transfer of genetic material from one organism to another?

  1. Gene Cloning

  2. DNA Fingerprinting

  3. Polymerase Chain Reaction

  4. Genetic Engineering


Correct Option: D
Explanation:

Genetic Engineering involves the deliberate modification of an organism's genetic material to introduce new traits or alter existing ones.

What is the name of the enzyme used to cut DNA at specific sequences?

  1. DNA Polymerase

  2. DNA Ligase

  3. Restriction Enzyme

  4. Helicase


Correct Option: C
Explanation:

Restriction Enzymes are enzymes that recognize and cut DNA at specific nucleotide sequences.

Which vector is commonly used for gene transfer in plants?

  1. Plasmid

  2. Bacteriophage

  3. Cosmid

  4. Ti plasmid


Correct Option: D
Explanation:

Ti plasmid is a naturally occurring plasmid found in Agrobacterium tumefaciens, which is used as a vector for gene transfer in plants.

What is the term for an organism that has had its genetic material altered using genetic engineering techniques?

  1. Transgenic Organism

  2. Recombinant Organism

  3. Genetically Modified Organism

  4. All of the above


Correct Option: D
Explanation:

Transgenic Organism, Recombinant Organism, and Genetically Modified Organism are all terms used to describe organisms that have had their genetic material altered through genetic engineering.

What is the process of inserting a foreign gene into a host organism's genome called?

  1. Gene Cloning

  2. Transformation

  3. Transduction

  4. Conjugation


Correct Option: B
Explanation:

Transformation is the process of introducing foreign DNA into a host organism's genome, typically through chemical or physical methods.

Which technique is used to amplify a specific DNA sequence?

  1. DNA Sequencing

  2. DNA Fingerprinting

  3. Polymerase Chain Reaction

  4. Southern Blotting


Correct Option: C
Explanation:

Polymerase Chain Reaction (PCR) is a technique used to amplify a specific DNA sequence by repeatedly copying it using a thermostable DNA polymerase.

What is the name of the technology that allows scientists to edit genes in living organisms?

  1. Gene Editing

  2. Genome Editing

  3. CRISPR-Cas9

  4. All of the above


Correct Option: D
Explanation:

Gene Editing, Genome Editing, and CRISPR-Cas9 are all terms used to describe the technology that allows scientists to make precise changes to the DNA of living organisms.

Which ethical concern is often raised regarding the use of genetic engineering in agriculture?

  1. Environmental Impact

  2. Food Safety

  3. Loss of Biodiversity

  4. All of the above


Correct Option: D
Explanation:

Environmental Impact, Food Safety, and Loss of Biodiversity are all ethical concerns that are often raised regarding the use of genetic engineering in agriculture.

What is the name of the international agreement that aims to ensure the safe and responsible use of biotechnology?

  1. Cartagena Protocol

  2. Kyoto Protocol

  3. Montreal Protocol

  4. Paris Agreement


Correct Option: A
Explanation:

The Cartagena Protocol on Biosafety is an international agreement that aims to ensure the safe and responsible use of biotechnology, particularly focusing on the transboundary movement of living modified organisms.

Which application of genetic engineering involves the production of therapeutic proteins in living organisms?

  1. Gene Therapy

  2. Protein Engineering

  3. Pharmaceutical Biotechnology

  4. All of the above


Correct Option: D
Explanation:

Gene Therapy, Protein Engineering, and Pharmaceutical Biotechnology are all applications of genetic engineering that involve the production of therapeutic proteins in living organisms.

What is the term for the process of transferring genes from one organism to another using a virus as a vector?

  1. Transduction

  2. Transformation

  3. Conjugation

  4. Gene Cloning


Correct Option: A
Explanation:

Transduction is the process of transferring genes from one organism to another using a virus as a vector.

Which technique is used to separate DNA fragments based on their size?

  1. DNA Sequencing

  2. DNA Fingerprinting

  3. Gel Electrophoresis

  4. Southern Blotting


Correct Option: C
Explanation:

Gel Electrophoresis is a technique used to separate DNA fragments based on their size by applying an electric current to a gel containing the DNA samples.

What is the term for the process of transferring genes from one organism to another using a bacterium as a vector?

  1. Transduction

  2. Transformation

  3. Conjugation

  4. Gene Cloning


Correct Option: C
Explanation:

Conjugation is the process of transferring genes from one organism to another using a bacterium as a vector.

Which application of genetic engineering involves the creation of organisms with desirable traits for agricultural purposes?

  1. Genetically Modified Crops

  2. Bioremediation

  3. Gene Therapy

  4. Pharmaceutical Biotechnology


Correct Option: A
Explanation:

Genetically Modified Crops are plants that have been altered using genetic engineering techniques to improve their traits, such as resistance to pests or herbicides.

What is the term for the process of transferring genes from one organism to another using a plasmid as a vector?

  1. Transduction

  2. Transformation

  3. Conjugation

  4. Gene Cloning


Correct Option: B
Explanation:

Transformation is the process of transferring genes from one organism to another using a plasmid as a vector.

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