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Oxidative phosphorylation - class-XI

Description: oxidative phosphorylation
Number of Questions: 70
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Tags: biology life processes in living organisms- part 1 biological oxidation respiration energy and respiration bio-chemistry respiration in plants
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Chemiosmosis hypothesis given by Peter Mitchell proposes the mechanism of

  1. Synthesis of NADH.

  2. Synthesis of ATP.

  3. Synthesis of FADH$ _2$.

  4. Synthesis of NADPH.


Correct Option: B
Explanation:
Chemiosmosis or chemiosmotic theory is given by Peter Mitchell explains the synthesis of ATP in the chloroplast. During the process of photosynthesis, the synthesis of ATP causes proton gradient to develop due to the accumulation of protons in thylakoid lumen that are formed by splitting of water. As the electron moves through the photosystem, protons are transported across the membrane. Now NADP reductase (enzyme) in stroma along with the electron acceptor removes protons along with the reduction of NADPH + H+. This causes proton gradient across the thylakoid membrane. The ATPase enzyme has a channel which allows the flow of proton through it and during this process the ATP is synthesised. Thus the correct answer is option B.

Riboflavin is essential in our diet, as it is required for the synthesis of

  1. TPP

  2. COASH

  3. NAD

  4. FAD


Correct Option: D

Major function of respiration is to produce

  1. NADH $(H^+)$

  2. ATP

  3. Pyruvate

  4. $C _2H _5OH$


Correct Option: B
Explanation:

Respiration is a process in which respiratory substrates are broken down through oxidation accompanying with the release of energy and its storage or conservation in the form of ATP with the release of carbon dioxide as a consequence. The actual mechanism of respiration is a stepwise process in which each step is catalyzed by a specific enzyme and the energy stored in the substrate is released in a stepwise series of reactions. The energy released in the process is used up in various energy-requiring processes of organisms. 

Thus, the correct answer is option B. 

Formation of ATP in respiration is called

  1. Photophosphorylation

  2. Substrate phosphorylation

  3. Oxidative phosphorylation

  4. Phosphorylation


Correct Option: C
Explanation:

The respiratory breakdown of glucose in the presence of oxygen is an oxidative process. During this process, several intermediates such as pyruvic acid, isocitric acid, succinic acid and oxalic acid are oxidized. Each oxidation step involves the release of 2 H which goes to reduce various coenzymes i.e. NAD+ and FAD. Reduced NAD+ and FAD released in the glycolysis and Krebs cycle finally reduce oxygen to water. This transfer of H+ and e- from NADH + H+ or FADH2 to oxygen is not a simple process and the direct transfer of electrons from coenzymes to oxygen is thermodynamically not possible. To facilitate this transfer, many intermediate cytochromes and other carriers are arranged in a series which transport electrons from NADH or FADH2 to oxygen. This sequence of electron carriers constitutes electron transport system. The electron transport proceeds from carriers that have low redox potential to those having high redox potential. The electron transport down to the energy gradient through electron transport system leads to the formation of ATP from ADP and inorganic phosphate. This generation of ATP is called oxidative phosphorylation. 

Thus, the correct answer is option C. 

Oxidative phosphorylation of cytoplasmic $NADH (H^+)$ takes place in

  1. Cytosol

  2. E.R.

  3. Mitochondria

  4. Golgi bodies


Correct Option: C
Explanation:

A large amount of NADH produced during glycolysis and Krebs cycle undergoes oxidation with phosphorylation of ADP to form ATP which is supported by mitochondrial electron transport assembly and ATP synthase which are integral protein complexes of the inner mitochondrial membrane. The electron transport assembly is comprised of a series of protein complexes that catalyze sequential oxidation-reduction reactions.

Thus, the correct answer is option C.

During carbohydrate metabolism, NADH is produced in which of the following locations?
I. Cytosol
II. Mitochondrial matrix
III. Nucleus.

  1. I only

  2. II only

  3. I and II only

  4. I, II and III


Correct Option: C
Explanation:

In  the cytoplasm, one glucose molecule breaks down into two pyruvate molecules, and creates two NADH molecules by glycolysis. Transformation of one pyruvate from cytosol to one acetyl CoA produces one NADH molecule in the mitochondrial matrix. During  each turn of Krebs cycle, each acetyl CoA is systematically processed through the cycle and produces three NADH molecules.

So, the correct option is 'I and II only'. 

Oxidation of one molecule of glucose yields 38 mols of ATP in the proportion of

  1. 8 in cytosol and 30 in mitochondrion

  2. 30 in cytosol and 8 in mitochondrion

  3. 38 in cytosol 

  4. 38 in mitochondrion


Correct Option: A
Explanation:
 Process  Site  Energy Release
 Glycolysis(breakdown of glucose into pyruvic acid)  In cytoplasm  2 ATP2 NADH
Decarboxylation(conversion of pyruvic acid into Acetyl co enzyme) mitochondria  2 ATP
 CItric Acid Cycle(Oxidation of acetyl co enzyme)(When cycle runs twice) mitochondria  8 NADH2 FADH2 GTP
 Total    10+28=38
So the correct option is "8 in cytosol and 30 in mitochondria".

The role of mitochondria in oxidative phosphorylation was explained by 

  1. Leninger

  2. Embden

  3. Krebs

  4. Meyer hoff


Correct Option: A
Explanation:

  • Oxidative phosphorylation is the metabolic pathway in which cells use enzymes to oxidize nutrients, thereby releasing energy which is used to produce ATP. In most eukaryotes, this takes place inside mitochondria. This was explained by Leininger
Thus, the correct answer is 'Leininger.'

In Kreb's cycle, how many oxidation (dehydrogenation) occur?

  1. 4

  2. 6

  3. 2

  4. 1


Correct Option: A
Explanation:

In Kreb's cycle the oxidation or dehydrogenation reaction occurs 4 times as follows:

  1. Isocitrate to alpha-ketoglutarate (NADH is formed)
  2. alpha-ketoglutarate to succinyl co-A (NADH is formed)
  3. Succinate to Fumarate (FADH$ _{2}$ is formed)
  4. Malate to oxaloacetate (NADH is formed)
Hence, the correct answer is '4'.

Which is formed in oxidative phosphorylation?

  1. ATP in photosynthesis

  2. ATP in respiration

  3. $NADPH _2$ in photosynthesis

  4. $NADPH _2$ in respiration.


Correct Option: B
Explanation:

Oxidative phosphorylation-


This process takes place in mitochondria. In this process ATP is formed when electron are transferred from 

NADH or $FADH _2$ to $O _2$

(B) ATP in respiration occurs or is formed in oxidation phosphorylation.

Two copper centers are component of

  1. Cytochrome $bc _1$ complex

  2. NADH dehydrogenase complex

  3. ATP synthase complex

  4. Cytochrome c oxidase complex


Correct Option: A
Explanation:

Option 'A' is correct.


Two copper centres two $W _a$ and $W _b$ are component of cytochrome $bc$, complex. $W _a$ contains two copper cone which are linked by two bridging cysteine residue, whereas $W _b$ is linked by there histidine residues.

How any molecules of water should be photolysed to form a $NADPH 2$ molecule ________.

  1. 4

  2. 2

  3. 6

  4. 1


Correct Option: A

In which of the following phosphorylation in absent?

  1. Glycolysis

  2. Kreb cycle

  3. $C _4$ cycle

  4. ETS


Correct Option: C
Explanation:

$C _4$ cycle or the Hatch-Slack pathway is a photosynthetic process in some plants. It is the first step in extracting carbon from carbon dioxide to be able to use it in sugar and other biomolecules. $C _4$ plants do not undergo phosphorylation due to their special mechanism to increase the $CO _2$ level for enzyme binding.

So, the correct answer is '$C _4$ cycle'.

Which of the following is a coenzyme?

  1. NAD

  2. NADP

  3. FAD

  4. All the above


Correct Option: D
Explanation:

Co-enzymes are organic compounds, are bound to the the enzyme to make the enzyme catalytically active, but their association with the enzyme is only transient, usually occurring during the course of catalysis. Co-enzymes serve as co-factors in a number of different enzyme catalyzed reactions. The essential chemical components of many coenzymes are vitamins, e.g., coenzyme nicotinamide adenine dinucleotide (NAD) and NADP contain the vitamin niacin; flavin adenine dinucleotide (FAD) is derived from riboflavin vitamin.

All of the following is true about oxidative phosphorylation, except

  1. It occurs in the inner membrane of the mitochondrion.

  2. It involves O$ _2$ as the final electron acceptor.

  3. It produces 2 ATPs for each FADH$ _2$.

  4. It can occur under anaerobic conditions.

  5. It involves a proton gradient.


Correct Option: D
Explanation:

Oxidative phosphorylation is an ATP generating process, which takes place in the inner membrane of mitochondria of aerobic organisms. In a generic sense, this process generates ATP via transferring electrons from $FADH _2$ or NADH via a series of complex carriers. Oxygen is a vital constituent of this process for its role as the final electron acceptor. Therefore, oxidative phosphorylation cannot take place in absence of oxygen. Hence, the correct answer is (D).

Which of the following is produced by oxidative phosphorylation?

  1. Oxygen and water

  2. NADH and ATP

  3. Pyruvate and NADPH

  4. Water and ATP

  5. Oxygen and NADH


Correct Option: D
Explanation:

The metabolic process of releasing energy from food can be termed as oxidative phosphorylation. It occurs in the mitochondria of eukaryotic cell. In this process, the electrons are transported through different carriers ultimately to the oxygen molecule. these electrons reduce oxygen to form water and release energy which converts ADP to ATP. 

ATP is produced by oxidative phosphorylation in

  1. Glycolysis.

  2. Krebs cycle.

  3. Chemiosmosis.

  4. B and C only.

  5. A, B and C.


Correct Option: C
Explanation:
Glycolysis and Kreb's cycle form total 4 ATPs by substrate level phosphorylation wherein the direct transfer of phosphate group to ADP or GDP during chemical reactions leads to the synthesis of ATP/GTP. Oxidative phosphorylation is the process wherein oxidation of reducing equivalent generate energy to drive ATP synthesis. Chemiosmosis includes the use of proton gradient generated across the inner mitochondrial membrane, during transfer of electrons from NADH and FADH$ _2$ to O$ _2$, to drive ATP synthesis. During chemiosmosis, 10 molecules of NADH power synthesis of 10*3 = 30 ATP while 2 molecules of FADH$ _2$ power synthesis of 2*2 = 4 ATP.
Thus, the correct answer is option C.

Electron carriers such as nicotinamide adenine dinucleotide (NAD) are used in energy capturing processes such as photosynthesis and respiration. They play a central role in the redox reactions that occur at the time of energy harvest from the carbon source.
What is the function of an oxidizing agent?

  1. Molecules that donate electrons.

  2. A molecule that becomes oxidized.

  3. A molecule that gains electrons.

  4. Molecules that combines with oxygen.


Correct Option: C
Explanation:

NAD$^+$ accepts electrons/H$^+$ released by water.

So, the correct answer is 'A molecule that gains electrons'

Enzymes required for phosphorylation are located in .......... of chloroplast.

  1. Peristromium

  2. Plastidome

  3. Stroma

  4. Quantosome


Correct Option: C
Explanation:

In chloroplast, the space enclosed by the inner membrane is called the stroma. It is colorless and homogeneous fluid. Phosphorylation is the process of addition of a phosphate group to ADP. The process of non-cyclic photophosphorylation takes place in the stroma lamellae with the help of enzymes present in it. Thus, the correct answer is option C. 

Centre of phosphorylation is

  1. Ribosome

  2. Oxisome

  3. Peroxisome

  4. Spherosome.


Correct Option: B
Explanation:

  • Oxidative phosphorylation refers to the process by which ATP molecules (energy currency) are produced in the mitochondria.
  • ATP is produced by the transfer of electrons from NADH or FADH2 to oxygen molecule with the help of electron carriers.
  • Oxysomes or F0-F1 particles refers to small round structures present within the folds of the cristae of the inner mitochondrial membrane.
  • F0 and F1 particles are found in the inner mitochondrial region and are attached to the cristae and help in ATP production and oxidation.
So, the correct answer is 'Oxisome'.

Terminal oxidation comprises

  1. Synthesis of metabolic water

  2. Electron transport

  3. Oxidative phosphorylation

  4. All the above


Correct Option: D
Explanation:

The respiratory breakdown of glucose in the presence of oxygen is an oxidative process. During this process, several intermediates such as pyruvic acid, isocitric acid, succinic acid and oxalic acid are oxidized. Each oxidation step involves the release of 2 H which goes to reduce various coenzymes i.e. NAD+ and FAD. Reduced NAD+ and FAD released in the glycolysis and Krebs cycle finally reduce oxygen to water. This transfer of H+ and e- from NADH + H+ or FADH2 to oxygen is not a simple process and the direct transfer of electrons from coenzymes to oxygen is thermodynamically not possible. To facilitate this transfer, many intermediate cytochromes and other carriers are arranged in a series which transport electrons from NADH or FADH2 to oxygen. This sequence of electron carriers constitutes electron transport system. The electron transport proceeds from carriers that have low redox potential to those having high redox potential. The electron transport down to the energy gradient through electron transport system leads to the formation of ATP from ADP and inorganic phosphate. This generation of ATP is called oxidative phosphorylation. 

Thus, the correct answer is option D. 

Kreb's cycle takes place in

  1. Vesicles of E.R

  2. Mitochondria

  3. Dictyosomes

  4. Chloroplasts


Correct Option: B
Explanation:

Kreb cycle is also known as the citric acid cycle or tricarboxylic acid cycle takes place in the inner membrane of mitochondria. It occurs there because the necessary enzyme for the Krebs cycle, succinic dehydrogenase is only found in the inner membrane of the mitochondria.

This is the only enzyme that has FAD as coenzymes and thus there is a formation of FADH₂ molecule.
Mitochondria are only associated with aerobic respiration.
So, the correct option is 'Mitochondria'

When a pair of electrons from NADH+$H^+$ transported through respiratory ETS, results in the formation of 

  1. 2 ATPs

  2. 4 ATPs

  3. 3 ATPs

  4. 5 ATPs


Correct Option: C
Explanation:
  • Glycolysis and Krebs cycle yield certain reduced coenzymes like NADH+H+ and FADH2.
  • ETS is metabolic pathway of electron transport that oxidizes these coenzymes to release energy from them.
  • NADH+H+ gets oxidized at Complex I of ETS and as the electrons are transferred to other Complexes, it creates a proton gradient for ATP production. One NADH+H+ yields 3 ATPs and one FADH2 yields 2 ATPs.
  • So the correct answer is '3 ATPs'.

Mitochondria are sites of

  1. Oxidative phosphorylation

  2. Photolysis

  3. Phosphorylation

  4. Starch synthesis.


Correct Option: A
Explanation:

The electron transport system is located in the inner membrane of the mitochondria.

The oxisomes or  F0-F1 particles are responsible for the transport of electrons. and ATP synthesis which takes place through electron transport system.
It is known as oxidative phosphorylation which is the addition of a phosphate group to ADP to form ATP in presence of oxygen.

So, the correct answer is 'Oxidative phosphorylation'

Oxidative phosphorylation is found in

  1. Chloroplasts

  2. Leucoplasts

  3. Peroxisomes

  4. Mitochondria


Correct Option: D
Explanation:

The process of formation of ATP during oxidation of reduced coenzymes in the electron transport chain is known as oxidative phosphorylation. This process takes place in the mitochondrial membrane because in mitochondrial membrane there are specialized particles which are known as oxysomes which contain coenzymes for electron transfer.

So, the correct option is 'Mitochondria'.

Oxidative phosphorylation is production of

  1. ATP in photosynthesis

  2. NADH in photosynthesis

  3. ATP in respiration

  4. NADH in respiration


Correct Option: C
Explanation:

Formation of ATP from ADP in the presence of oxygen during the Krebs cycle is known as oxidative phosphorylation.

This takes place in mitochondria of the cell during oxidative phosphorylation a total of 28 ATP are formed.
So the correct option is 'ATP in respiration'

ATP is produced during

  1. Cyclic photophosphorylation

  2. Non cyclic photophosphorylation

  3. Both (A) and (B)

  4. None of the above


Correct Option: C
Explanation:

ATP is produced during both cyclic and non-cyclic photophosphorylation. During non-cyclic photophosphorylation when electrons move from PS II to PS I, then ATP and $NADPH _2$ is generated and during cyclic photophosphorylation only ATP is generated.

A molecule of $FADH 2$ _____________.

  1. Consumes one $O _2$

  2. Consumes one $H2 _O$ molecule

  3. Forms 2 ATP

  4. All the above


Correct Option: C

Which is product of oxidative pentose phosphate pathway?

  1. Pyruvic acid

  2. Acetyl CoA

  3. NADH$ _2$

  4. NAD(P)H

  5. ATP


Correct Option: D
Explanation:

Pentose phosphate pathway or HMP is an oxidative phosphate pathway of cellular respiration in which glucose 6-phosphate acts as the substrate. After its complete oxidation through HMP, 12 NADPH + H+ and 6 CO2 are formed. This pathway is an alternate pathway to glycolysis and is seen in RBC, adrenal cortex, liver, etc. A net gain of 35 ATP is achieved through this pathway.

So the answer is 'NAD(P)H'.

How many ATP molecules can be produced through oxidative phosphorylation of 2 $NADH 2$ and 3 $FADH _2$ _______.

  1. 15

  2. 24

  3. 6

  4. 12


Correct Option: D

One molecule of NADH and one molecule of $FADH _2$ yield

  1. 2 and 3 ATP

  2. 18 and 36 ATP

  3. 36 and 18 ATP

  4. 3 and 2 ATP

  5. 2 and 36 ATP


Correct Option: D
Explanation:

Reduced molecules like NADH+H+ and FADH2 yield energy in the form of ATP when they undergo ETC in mitochondria. NADH+H+ enter ETC at higher complex than FADH2 so they release energy as 3 ATP and 2 ATP respectively.

So the answer is '3 and 2 ATP'.

FAD is electron acceptor in citric acid cycle during the oxidation of

  1. Malic acid to oxaloacetatic acid.

  2. Succinic acid to malic acid.

  3. Citric acid to -ketoglutaric acid.

  4. $\alpha$-ketoglutaric acid to succinic acid.


Correct Option: B
Explanation:

The Krebs cycle is the condensation of acetyl group with oxaloacetic acid and water to yield citric acid and leads to the formation of Oxalo acetic acid.

Eighth step in which Succinic acid converts into Fumaric acid in the presence of enzyme succinic dehydrogenase release energy and form FADH which yields 2ATP.
So the correct option is 'Succinic acid to Fumaric acid.'

The process of phosphorylation takes place in

  1. Glycolysis

  2. Kreb's cycle

  3. HMP pathway

  4. All of the above


Correct Option: D
Explanation:

Phosphorylation is the addition of a phosphate (PO$ _{4}^{3-}$) group to a protein or other organic molecule. Phosphorylation results in the formation of ATP, which is the energy currency of cell. Phosphorylation occurs as a part of glycolysis, Kreb's cycle and HMP pathway.

Oxidative phosphorylation occurs during the process of

  1. Protein synthesis

  2. $N _{2}$ fixation

  3. Respiration

  4. Transpiration


Correct Option: C
Explanation:

Oxidative phosphorylation is a metabolic process, which is a part of respiration, which occurs in mitochondria. As a result of this process energy is released in the form of ATP.

In oxidative phosphorylation, oxidation and phosphorylation take place simultaneously and forms:

  1. NADP

  2. DPN

  3. Pyruvic acid

  4. ATP

  5. Both B and D


Correct Option: E
Explanation:

Oxidative phosphorylation refers to synthesis of ATP by ADP and Pi driven by energy released from oxidation of reducing powers NADH and FADH2 by transfer of their electrons through electron trasnport chain to oxygen. Electron trasnport chain is present in inner mitochondrial membrane and passes the electrons down a series of carriers and pump protons into the intermembrane space. Oxygen serves as final electron acceptor in oxidation of NADH and FADH2 and the resulting proton gradient serves as energy source for the enzyme ATP synthase to synthesize ATP. So final products of oxidative phosphorylation are ATP, NAD and FAD. DPN (diphosphopyridine nucleotide), also called as NAD. Correct answers are D and B. NADP is coenzyme of photosynthetic light reactions. Puruvic acid is product of glycolysis. 

Oxidative phosphorylation is the formation of 

  1. NADPH$ _{2}$ in respiration

  2. ATP in respiration

  3. NADPH$ _{2}$ in photosynthesis

  4. ATP in photosynthesis


Correct Option: B
Explanation:

Oxidative phosphorylation is the metabolic pathway in which the mitochondria in cells use their structure, enzymes and energy released by the oxidation of nutrients to reform ATP in respiration.

Biological phosphorylation is the formation of 

  1. $N _{2}$

  2. $CO _{2}$

  3. $ATP$

  4. $SO _{2}$


Correct Option: C
Explanation:

Biological phosphorylation is of two types- protein phosphorylation and oxidative phosphorylation. In protein phosphorylation, the proteins get attached with a phosphate group. Oxidative phosphorylation is the metabolic pathway in which the mitochondria in cells use their structure, enzymes, and energy released by the oxidation of nutrients to reform ATP.

$FAD^+$ is reduced during conversion of 

  1. Citric acid to isocitric acid

  2. Isocitric acid to $ \alpha - $ ketoglutaric acid

  3. $ \alpha - $ ketoglutaric acid to succinyl CoA

  4. Succinic acid to fumaric acid


Correct Option: D
Explanation:

During one of the steps of Krebs cycle, succinic acid is converted to fumaric acid. The reaction is accompanied by coenzyme FAD (flavin adenine nucleotide). This oxidation reaction liberates less free energy as compared to other oxidation reactions. FAD$^+$ is the hydrogen acceptor in this reaction as there is very less free-energy change. In this reaction, succinic acid is oxidized to fumaric acid. At the same time, FAD$^+$ is reduced to FADH$ _2$. 

Thus, the correct answer is option D.

During aerobic respiration, the substrate level phosphorylation takes place in.

  1. Cytoplasm only

  2. Mitochondria only

  3. Cytoplasm and mitochondria

  4. Cytoplasm and Golgi complex


Correct Option: A


Within the mitochondrion, the proton gradient develops across the

  1. Outer membrane

  2. Inner membrane

  3. Intermembrane space

  4. Matrix


Correct Option: B
Explanation:

The electrons from NADH AND FADH$ _2$ flows through the inner mitochondrial membrane, thus forming a proton gradient i.e; building an H+ pump which flows through the membrane enzyme complex. An enzyme responsible for this process and energy generation is ATP synthase.

So, the correct option is ' inner membrane'.

Choose the correct answers from the alternatives given :
Chemiosmotic phosphorylation is dependent upon

  1. the ability of ADP? to join with P even in the absence of a supply of energy

  2. the establishment of an electrochemical hydrogen ion $(H^+)$ gradient

  3. an outside supply of phosphate and other chemicals

  4. the diffusion of waste across a differentially permeable membrane


Correct Option: B
Explanation:

  • Chemiosmotic phosphorylation is the process where ATP is formed by the ATP synthase complex which is present in the inner membrane of the mitochondria.
  • The formation of ATP by this complex takes place when 2 hydronium ions are transported across the complex from the inter membrane space down its concentration gradient.
  • Thus the formation of ATP in this process takes place due to the concentration gradient that is formed by the presence of hydronium ion that is formed by the ETS Chain when the electron along with hydronium ions are transported from the NADH and FADH.
  • Therefore the answer 'the establishment of an electrochemical hydrogen ion (H+) gradient' is correct.










Choose the correct answers from the alternatives given :
Substrate-level phosphorylation takes place in 

  1. Krebs cycle and the transition reaction

  2. glycolysis and the electron transport system

  3. electron transport system and the transition reaction

  4. glycolysis and the Krebs cycle


Correct Option: D
Explanation:
  • Substrate level phosphorylation is the reaction where ATP is formed by the phosphate molecule that is taken from the substrate in the reaction.
  • In glycolysis during the conversion of 1,3 bisphosphoglyceric acid to 3 phosphoglyceric acid and coversion of phosphophenol to pyruvic acid; one molecule of ATP is formed during each reaction.
  • In kerb cycle during the formation of succinic acid from succinyl CoA GTP is formed which is a substrate level phosphorylation, afterwards this GTP is converted to ATP in a coupled reaction.
  • No substrate level phosphorylation takes place in oxidative phosphorylation or ETS
  • Therefore the answer 'glycolysis and the Krebs cycle' is correct.

Choose the correct answers from the alternatives given :
Which of the following occurs only in aerobic conditions?

  1. Glycolysis

  2. Lactate respiration

  3. Oxidative phosphorylation

  4. Fermentation


Correct Option: C
Explanation:

  • Glycolysis is the first part of the reaction that provides substrate for the further reaction of aerobic and anaerobic reaction. IT takes place in cytoplasm and does not require any oxygen.
  • LActate respiration and fermentation both are the reaction that take place in the cells or the tissues which respiration in anaerobic conditions or when there is not enough oxygen.
  • Oxidative phosphorylation is the process that takes place in the in the inner mitochondrial membrane where the electrons and hydronium ions are transported through a series of complexs and at the end the electrons and hydronium are accepted by the oxygen and water is formed, this is the process in the aerobic respiration where the oxygen is used and it cannot be completed with it.
  • Therefore the answer option 'Oxidative phosphorylation' is correct.







Choose the correct answers from the alternatives given :
The direct energy source that drives ATP synthesis during oxidative phosphorylation is 

  1. a difference of $^H+$ concentration on opposite sides of the inner mitochondrial membrane

  2. the affinity of oxygen for electrons

  3. oxidation of glucose and other organic compounds

  4. endergonic flow of electrons down the electron transport chain


Correct Option: A
Explanation:

  • In mitochondria the ETS is used for the transport of electrons and hydronium ions from inner mitochondrial membrane to the inter membrane matrix of mitochondria.
  • Due to this the concentration of hydronium ions increases in the intermembrane matrix.
  • The ATP synthase complex which is present in the inner membrane of the mitochrondria are used for the synthesis of ATP.
  • The complex is used to transport hydronium ions from the intermembrane matrix down its concentration gradient
  • For every 2 hydronium ions transported through the complex one molecule of ATP is generated.
  • Therefore the transport of the H+ ions due the difference in the concentration provides direct energy for the formation of ATP.
  • Therefore the correct answer is option 'a difference of H+ concentration on opposite sides of the inner mitochondrial membrane'.









Choose the correct answers from the alternatives given :
The final electron acceptor of the electron transport chain that functions in oxidative phosphorylation 

  1. pyruvic acid

  2. oxygen

  3. water

  4. $NAD^+$


Correct Option: B
Explanation:

  • Electrons that are transported through the ETS are passed through various complexs of the transport chain such as complex I, cytochrome c cytochrome b, etc
  • When the electrons are passed through the complexs one after the other hydrogen ions are also passed through along with it.
  • After the electrons and the hydrogen have passed through the last of the complex the last acceptor of the electron in this chain will be a molecule of oxygen after reacting to which a molecule of water is formed.
  • Therefore the correct answer is option 'oxygen'.








Choose the correct answers from the alternatives given :
Most of the ATP made during cellular respiration is generated by 

  1. direct synthesis of ATP by the Krebs cycle

  2. substrate-level phosphorylation

  3. oxidative phosphorylation

  4. glycolysis


Correct Option: C
Explanation:
  • Oxidation phosphorylation is the process where the electron acceptor such as NADH and FADH release their H ions and the ions and the electrons are tranported through various complexs of electron transport system.
  • NADH gives electrons which pass through 5 complexs of the ETS and FADH pass through 4 complexs of the ETS 
  • These electron at the end of the transport system react with oxygen and form a molecule of water.
  • Where the proton which is released by the complexs of the ETS during the transport of electrons are used in formation of ATP.
  • one molecule of NADH forms 3 molecules of ATP and one molecule of FADH forms 2 molecules of ATP
  • So after one cycle of kerb 4 molecules of NADH are released and one molecule of FADH which results in formation of 12 ATP and 2 ATP respectively. Thus generating 14 ATP for each pyruvic acid.
  • Therefore the amount of the maximum generated ATP in cellular respiration is done by oxidative phosphorylation.
  • Therefore the answer option 'oxidative phosphorylation' is correct.
All of the following processes can release ${CO} _{2}$ except
  1. Alcoholic fermentation

  2. Oxidative decarboxylation and Krebs' cycle

  3. Oxidative phosphorylation

  4. Conversion of $\alpha$-ketoglutaric acid to succinic acid


Correct Option: C
Explanation:

The process of oxidative phosphorylation involves the production of ATP by utilizing electrons falling from the hydrogen in glucose to the oxygen in a living cell. It does not lead to the production of carbon dioxide molecules.

Thus, the correct answer is 'Oxidative phosphorylation.'

Read the given statements and select the correct option.
Statement 1: During photophosphorylation (of photosynthesis), light energy is utilised for the production of a proton gradient during ATP synthesis.
Statement 2: In respiration, the energy of oxidation-reduction is utilised for the phosphorylation and thus the process is called oxidative phosphorylation.
  1. Both statements 1 and 2 are correct.

  2. Statement 1 is correct but statement 2 is incorrect.

  3. Statement 1 is incorrect but statement 2 is correct.

  4. Both statements 1 and 2 are incorrect.


Correct Option: A
Explanation:

Photophosphorylation occurs in the grana and requires the direct sunlight energy to make energy-carrier molecules that are used in the dark reaction. The light energy is trapped by chlorophyll to make ATP and NADPH. Oxidative phosphorylation is the synthesis of energy-rich ATP molecules with the help of energy liberated during oxidation of reduced co-enzymes (NADH,${FADH} _{2}$) produced in respiration.

So the correct answer is 'Both statements 1 and 2 are correct'.

Which one is not correct for oxidation of food?

  1. It is multiple process

  2. All released energy is traped in form of ATP

  3. Traped energy is further used for bio synthesis of other molecules

  4. Oxidisable compound is known as respiratory substrate


Correct Option: B
Explanation:

During the oxidation of carbon atoms from complex organic food molecule, carbon dioxide is released as a waste product. The energy released from these reactions is not used up by the cells completely. Alternatively, it is converted into ATP and NADH, and this can be used throughout the cell to power metabolism and construct new cellular components. In addition, workhorse proteins called enzymes use this chemical energy to catalyze chemical reactions within the cell.

So, the correct option is 'All released energy is trapped in form of ATP'.

During oxidative phosphorylation, proton return to

  1. Matrix from outside

  2. Outside from matrix

  3. In both directiion

  4. Mitochondria to cytoplasm


Correct Option: A
Explanation:

Oxidative phosphorylation is the process of formation of ATP (energy) molecules inside the plant and animal cells. It takes place inside the mitochondria. During the oxidative phosphorylation, electrons flow from NADH  or FADH to Othrough the four different type complexes found in the inner mitochondrial membrane, which leads the pumping of the proton from outside to the matrix.

So, the correct answer is ' Matrix from outside '

During cyclic photophosphorylation proton gradient established by

  1. $PQ$ mediated $H^+$ transfer

  2. Photolysis of $H _2O$

  3. $NADP$ reductase mediated withdrawal of $H^+$

  4. All of the above


Correct Option: A
Explanation:

The proton gradient produced by proton pumping during the electron transport chain is used to synthesize ATP. Protons flow down their concentration gradient into the matrix through the membrane protein ATP synthase, causing it to spin & catalyze the conversion of ADP to ATP.

So, the correct option is 'PQ mediated H+ transfer'.

Correct answer(w.r.t. ETS and oxidative phosphorylation in mitochondria):
(i) $F _1$ headpiece is a peripheral membrane complex and contains site of ATP synthesis
(ii) Passage of electrons through channel is coupled to catalytic site of $F _0$ component for ATP production.
(iii) $F _0$ is integral membrane protein  complex that forms the channel through which protons cross inner membrane
(iv) Cyt c is small protein attached to inner surface of inner mitochondrial membrane.
*

  1. (i) and (iii) are correct

  2. (ii) and (iii) are incorrect

  3. (iii) and (iv) are correct

  4. (i) and (i)v are incorrect


Correct Option: A

In case NADH is oxidised in a single step to form water.

  1. Cell will burn

  2. Most of energy is liberated as heat

  3. $3$ ATP are formed

  4. $5$ ATP are formed


Correct Option: B
Explanation:

NADH is reoxidized in a single step to form water (in the presence of oxygen). NADH to donate electrons directly to Oxygen to water. NADH to Oxygen the many-electron carriers in the electron transport chain and heat is liberated as most of the energy.

So the correct answer is 'Most of the energy is liberated as heat'.

NADH is oxidised to $NAD^+$ in

  1. Aerobic respiration

  2. EMP pathway

  3. Fermentation

  4. None of these


Correct Option: A,C

If ATP was unavailable to the cell, as would be the case when treated with an uncoupler of oxidative phosphorylation, the effects would be numerous. Among them, $Na^+/K^+$ ATPase function would be inhibited.
What would be a consequence of such an inhibitory action?

  1. Inhibiting the $Na^+/K^+$ pump would cause a cessation of all ion movement.

  2. Sodium would leak into the cell and potassium would leak out of the cell, down their electrochemical gradient.

  3. The ion concentration in the cell would "drift" towards that of the intercellular fluid.

  4. Water would flow out of the cell leading to crenation.


Correct Option: B
Explanation:

Na$^+$/K$^+$ ATPase, is a cationic pump that transports 3Na$^+$ ions to the outside, while importing 2K$^+$ against their concentration gradient. This maintains the inner membrane potential negative, In inhibition of the pump, the ions will move along their concentration gradient, reaching towards equilibrium through the leak channels that allow passive movement of these ions.

So, the correct answer is 'Sodium would leak into the cell and potassium would leak out of the cell, down their electrochemical gradient'

The function of ATP synthase enzyme is that

  1. It uses energy to move ions against their concentration gradient by removing a phosphate group from ATP.

  2. It uses the power of ions moving against their concentration gradient to add a phosphate group to ADP.

  3. It uses the power of ions moving down their concentration gradient to add a phosphate group to ADP.

  4. It uses the power of ions moving against their concentration gradient to remove a phosphate group from ATP.

  5. It uses the power of ions moving down their concentration gradient to remove a phosphate group from ATP.


Correct Option: C
Explanation:

Electron transport chain includes the downhill flow of electrons to final electron acceptor through a chain of membrane-bound carriers to facilitate the uphill transport of protons across a proton-impermeable membrane. It produces proton gradient that drives transport of protons (ions) down the concentration gradient through Fo particles of ATP synthase. The proton-motive force that drives movement of protons (ions) provides the energy for ADP phosphorylation. Thus, the correct answer is option C.

NAD of Krebs cycle functions as

  1. Acceptor of hydrogen ion and electrons

  2. Oxygen acceptor

  3. Oxygen donor

  4. Donor of phosphate ions


Correct Option: A
Explanation:

NAD is a coenzyme which acts as an electron acceptor in Krebs cycle and acquires hydrogen ions to become NADH.

So the correct option is 'Acceptor of hydrogen ion and electrons'.

Oxidative phosphorylation occurs during 

  1. Transpiration

  2. Respiration

  3. Protein synthesis

  4. Nitrogen metabolism


Correct Option: B
Explanation:

Oxydative phosphorylation is the process of formation of ATP as a result of transfer of electrons from NADH or FADH$ _2$ to O$ _2$. This process which takes place in mitochondria and ATP is the major source of cellular respiration.

So, the correct option is 'Respiration'. 

Enzymes of oxidative phosphorylation occur in

  1. Endoplasmic reticulum

  2. Chloroplasts

  3. Mitochondria

  4. Golgi apparatus


Correct Option: C
Explanation:

Enzymes that catalyze oxidative phosphorylation are present in the inner membrane which is much less permeable of mitochondria. Oxidative phosphorylation is the process of electrons are transferred from electron donors to electron acceptors 

So the correct option is 'Mitochondria'.

Which is end product of oxidative phosphorylation?

  1. ATP

  2. ATP + $H _2O$

  3. NADH

  4. Oxygen.


Correct Option: B
Explanation:

the process produces the 2 pyruvate molecule with 2 water molecule, 2 ATP, 2 NADH molecules, and 2 hydrogen ions. At the end of electron transport, oxygen is the final electron acceptor, and it combines also with hydrogen ions to form H2O. The end product of oxidative phosphorylation would be ATP molecules, which carry energy.

So the correct option is 'ATP + H$ _2$O'.

FAD is electron acceptor during oxidation of 

  1. Ketoglutarate Succinyl CoA.

  2. Succinic acid Fumaric acid.

  3. Succinyl CoA Succinic acid.

  4. Fumaric acid Malic acid.


Correct Option: B
Explanation:

The hydrogen acceptor is FAD during the oxidation of Succinic acid Fumaric acid by succinate dehydrogenase.

So the correct option is 'Succinic acid Fumaric acid.'

Differences between photophosphorylation (PP) and oxidative phosphorylation (OP) is 

  1. In PP, synthesis is of ATP while in OP it is of ADP.

  2. In PP, oxygen is evolved while in OP oxygen is taken up.

  3. Both cannot take place in light.

  4. PP can take place in green leaves while OP cannot occur in green leaves.


Correct Option: B
Explanation:

Both the oxidative phosphorylation and photophosphorylation are in which cells make energy in the form of ATP, oxidative phosphorylation occurs during cell respiration and photophosphorylation occurs during photosynthesis. Oxygen is released during photophosphorylation and oxygen is taken up.

So the correct option s 'In PP, oxygen is evolved while in OP oxygen is taken up'.

Which one is the last electron acceptor over ETC in oxidative phosphorylation?

  1. $H _2$

  2. $Cyt a _3$

  3. Cyt b

  4. $CO _2$


Correct Option: B
Explanation:

Iron and copper are present in cytochrome a$ _3$, Iron transfer electron to oxygen through copper, so cytochromea3 called terminal electron donor.

So the correct option is Cyta$ _3$.'

Mitochondrial electron transport chain is

  1. Cyclic phosphorylation.

  2. Oxidative phosphorylation.

  3. Noncyclic phosphorylation.

  4. Photooxidation.


Correct Option: B
Explanation:

The electron is transferred from NADH to oxygen in mitochondria con­sists of four multi-protein complexes is coupled to the synthesis of ATP from ADP and inorganic phosphate (Pi) which is called as oxidative phosphorylation. 

So the correct option is 'Oxidative phosphorylation.'

Coenzyme $NAD^+$ and FAD are connected with respiratory reactions as they

  1. Are involved in each step of ATP synthesis.

  2. Function in Krebs cycle and terminal oxidative phosphorylation.

  3. Act as hydrogen carrier.

  4. Are reducing agents.


Correct Option: C
Explanation:

NAD and FAD plays a major role in cellular respiration,  They carry the H+ and electrons to Electron Transport Chain to convert ADP + Pi ATP.

So the correct option is 'Act as a hydrogen carrier.'

Glyceraldehyde phosphate is oxidised in glycolysis. The hydrogen atom and electron liberated cause

  1. Oxidation of NAD$^+$.

  2. Reduction of NAD$^+$.

  3. Change in oxaloacetic acid.

  4. Formation of methane.


Correct Option: B
Explanation:

Glyceraldehyde-3-phosphate is oxidized by the coenzyme nicotinamide adenine dinucleotide (NAD). NAD+ is an oxidizing agent - it accepts electrons from other molecules and becomes reduced.

So the correct option is 'Reduction of NAD+'.

Which of the following is a biological uncoupler of oxidative phosphorylation?

  1. Thermogenin.

  2. 2, 4-Dichlorophenoxyacetic acid.

  3. 2, 4-Dinitrophenol.

  4. Ethylene diaminotetra-acetic acid.


Correct Option: A
Explanation:

Brown adipose tissue is rich in mitochondria, containing an uncoupling protein (thermogenin) that uncouples oxidation and phosphorylation of adenosine diphosphate, reduces ATP production, and consequently enhances thermogenesis.

So the correct option is 'Thermogenin'.

Identify the wrong statement.

  1. Proton motive force drives the uniport.

  2. Movement of NO$ _3$ ions in cotransport is against their own concentration gradient.

  3. Azides inhibit the process of respiration.

  4. ATPase serves as proton-translocating carrier protein.


Correct Option: A

In mechanism, photophosphorylation is very similar to

  1. substrate-level phosphorylation

  2. oxidative phosphorylation

  3. Calvin cycle

  4. glycolysis


Correct Option: B
Explanation:

Oxidative phosphorylation is the process in which ATP is formed by the transfer of electrons from NADH or FADH 2 to O 2 by a series of electron carriers. The sunlight-driven production of ATP from ADP and inorganic phosphate is called photophosphorylation.

So, the correct option is, 'oxidative phosphorylation'.

FAD acts as an electron acceptor in between

  1. fumaric and mallic acid

  2. succinic and fumaric acid

  3. malic and oxaloacetic acid

  4. citric and isocitric acid


Correct Option: B
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