Genetic modification and biotechnology - class-VII
Enzyme' Taq polymerase' used in PCR, has been isolated from bacterium _________________.
The polymerase chain reaction is a technique used for ____________.
Which of the following statements are correct for the enzyme Taq polymerase?
Process used for amplification or multiplication of DNA in DNA fingerprinting is
Primers are
Given table gives an account of differences between PCR and gene cloning. Which of the, following points shows the incorrect difference?
Parameter | PCR | Gene cloning | |
---|---|---|---|
1 | Efficient | More | Less |
2 | Apparatus Requirement | DNA | Restriction enzyme, ligase, vector, bacterial cell |
3 | Manipulation | In vitro | In vitro and in vivo |
4 | Cost | More | Less |
5 | Automation | Yes | No |
6 | Error probability | Less | More |
7 | Time for a typical experiment | 2-4 days | 4 hours |
8 | Application | More | Less |
In a polymerase chain reaction, temperature required for the steps (i) Denaturation,(ii) Annealing, and (iii) Extension are respectively.
In addition to Taq polymerase enzyme which other thermostable DNA polymerases have been isolated to be used in polymerase chain Reaction (PCR)?
Which one of the following is not a correct match?
The correct sequence of different steps of polymerase chain reaction is
A doctor while operating on an HIV(+)ve patient accidentally cuts himself with a scalpel. Suspecting himself to have contracted the virus which test will he take to rule out/confirm his suspicion?
Given figures represent the steps involved in polymerase chain reaction (PCR). Identify the steps A, B, C and D, and select the correct option.
A | B | C | D | |
---|---|---|---|---|
a | Denaturation at 94-96 degree C | Extension through Taq polymerase at 72 degree C | Repetition of denaturation and annealing | Annealing at 72 degree C |
b | Denaturation at 94-96 degree C | Annealing at 40-60 degree C | Extension through Taq polymerase at 72 degree C | Repetition of denaturation and annealing |
c | Denaturation at 40-60 degree C | Annealing at 72 degree C | Extension through Taq polymerase at 94-96 degree C | Repetition of denaturation and annealing |
d | Extension through Taq polymerase at 72 degree C | Denaturation at 40 degree C | Annealing at 72 degree C | Repetition of denaturation and annealing |
Study the following statements regarding recombinant DNA technology and select the incorrect ones
Reaction of 1989 was
X technique is now routinely used to detect HIV in suspected AIDs patients. It is being used to detect mutations in genes in suspected cancer patients too. It is a powerful technique to identify many other genetic disorders. Identify X-
PCR or polymerase chain reaction was discovered by
The function of polymerase chain reaction (PCR) is
Polymerase chain reaction is useful in
DNA taq polymerase enzyme is isolated from which bacteria?
The whole cycle of gene amplification requires
Enzyme required for polymerase chain reaction (PCR) is
Thermal cycle is used in
One of the methods of which DNA cannot be transferred to the host cell is by
Polymerase chain reaction
During PCR technique the pairing of primers to ssDNA segment is called
How many copies of the DNA sample are produced in PCR technique after 6-cycle?
Which of the following is not necessary to execute polymerase chain reaction successfully?
In reference to DNA polymerase III, which statement is wrong?
Polymerisation of DNA is in
In PCR, short primers are added to single stranded DNA molecules in a test tube and the appropriate enzymes are included to make a copy of the DNA. Which of the following primers is needed for copying the single stranded DNA sequence $ 5'TACGGTAGGTC3'$ ?
A DNA sequencing reaction was performed with the fragment $5-XXXGCGATCGYYYY-3'$ as the template, dideoxy GTP, all the four $dNTPs$, and the required primers and enzyme. $XXXX$ and $YYYY$ in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be (the primers are not shown in the amplified fragments).
Column I Column II
I. Agarose A. PCR
II. Opines B. Gene gun
III. Biolistic C. Ti plasmid
iv. Thermal cycler D. Sea weeds
The areas of application of PCR include
B. Insertion of recombinant DNA into an organism
C. Diagnosis of specific mutation
D. Detection of plant pathogens
Which of the following can't be achieved using PCR ?
First technique and product produced by biotechnology are:
Which of the following has popularised the PCR(Polymerase chain reactions)?
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
The polymerase chain reaction is a technique used for?
Which of the following technique is employed to check the progression of a restriction enzyme digestion?
Which of the following is not required in PCR?
The areas of application of PCR include:
A. Production of monoclonal antibodies.
B. Insertion of recombinant DNA into an organism.
C. Diagnosis of a specific mutation.
D. Detection of plant pathogens.
Which of the following are all Nucleotides?
Kary B Mullis got Nobel prize for discovering
One of the major advantages of transforming bacteria with a plasmid that contains DNA of interest is to amplify the DNA.
What other laboratory technique can be used to achieve the same result?
Who developed polymerase chain reaction technique and got Nobel Prize
Polymerase chain reaction is used for
PCR and RFLP are employed in
An analysis of chromosomal DNA using Southern hybridisation technique does not use
Which ones are simple sequence repeats (SSRs)
Polymerase chain reaction is useful in
Polymerase chain reaction employs
DNA polymerase or taq enzyme used in PCR is isolated from
Source of Taq polymerase used in PCR is a
Gene amplification using primers can be done by
PCR proceeds in three distinct steps governed by temperature. The order of the steps is
What is true about DNA polymerase used in PCR?
Melting of DNA at 70$^o$ C is due to breakdown of
Amplification of gene of interest by using PCR may go upto
At which temperature a DNA molecule is denatured by heat in PCR
Taq polymerase is isolated from a
Taq polymerase is isolated from which bacteria?
Polymerase chain reaction (PCR) is used for
Which one of the following is NOT a PCR-based molecular marker?
DNA is amplified by __________________.
Which one of the following enzymes is used in PCR reaction?
Polymerase chain reaction technique is used for.
The $C _2$ glycolate cycle is also known as.
If the initial number of template DNA molecules in a PCR reaction is $1000$, the number of product DNA molecules at the end of $20$ cycles will be closest to.
How many cycles of PCR are required if desired number of DNA molecules is 64 and starting material is single stranded DNA instead of ds DNA ?
Which does not describe a function of the DNA polymerase molecule?
To carry out a polymerase chain reaction (PCR), one must have the catalytic DNA polymerase and
What is the function of the polymerase chain reaction in genetic engineering?
In the $PCR$ technology the $DNA$ segment is replicated over a billion times. This repeated replication is catalyzed by the enzyme.
The taq polymerase enzyme is obtained from
Which one is a true statement regarding $DNA$ polymerase used in $PCR$
Which of the following steps is/are catalysed by Taq polymerase in a PCR?
Which among the following is true for protein synthesis in bacteria?
Select the wrong pair
Taq polymerase enzyme is used in
Which of the following would not be a valid reason to use PCR?
Thermal cycler is used in one of these reactions.
DNA polymerase required for synthesizing copies of DNA at high temperature is obtained from
Which of the following techniques is used to make numerous copies of a specific segment of DNA quickly and accurately?
Read the following statements carefully and select the correct option:
(A) Source of the restriction enzyme Hind III is E.coli
(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA
(C) Micro-injection method for injecting recombinant DNA is used for animal cell
(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.
How many of the above statement are correct?
Vegetative reproduction is most favourable with which part of plant.
Rules of conduct that may be used to regulate our activities in relation to the biological world is called ___________.
Which of the following group of preservatives prevent fat/oil containing food from developing a foul smell?
.......... was the first commercially grown genetically engineered food to be granted a license for human consumption.
Saffron and beetroot plant extract can be used as
While purchasing a sealed packet of ghee, one should look for which mark?
If the fermentation of molasses during the manufacturing of ethyl alcohol is delayed then the conclusion that can drawn is
What does G.M.O stand for?
A genetically manipulated organism containing in its genome one or more inserted gene of another species is called as
Gene from the Bacillus thuringiensis is introduced into soyabean.
The genetically modified (GM) brinjal in India has been developed for
The genetically modified crops (GM) may contain the gene of interest from
Maize hybrids have been developed for higher amount of
RR-21 is high yielding variety of
Which of the following is not a genetically modified crop plant?