0

Biotechnology in agriculture - class-XI

Attempted 0/94 Correct 0 Score 0

$T _i$ plasmid used for making transgenic plants belongs to.

  1. Clsotridium

  2. Saccharomyces

  3. Agrobacterium

  4. Escherichia


Correct Option: C
Explanation:

Ti plasmid used for tumerogenesis in making transgenic plants belong to Agrobacterium tumificiens.

A good vector in genetic engineering is

  1. Agrobacterium tumefaciens

  2. Bacillus thuringiensis

  3. Bacillus amyloliquefaciens

  4. Salmonella typhimurium


Correct Option: A

Plasmids are used in genetic engineering because they are

  1. Easily available

  2. Able to replicate

  3. Able to integrate with host chromosome

  4. Inert


Correct Option: B

Bacterium commonly used in plant genetic engineering is

  1. Agrobacterium

  2. Corynebacterium

  3. Bacillus subtilis

  4. Salmonella typhii


Correct Option: A

Tumour inducing plasmid used in producing transgenic plants is that of ________________.

  1. Escherichia coli

  2. Bacillus thuringiensis

  3. Agrobacterium tumefaciens

  4. Staphylococcus aureus


Correct Option: C

Which statement is wrong?

  1. Nucleic acid is fragmented by nucleases

  2. Construction of recombinant DNA involves cleaving DNA segments with endonuclease and rejoining with ligase.

  3. Genetic engineering is making artificial limbs and diagnostic instruments

  4. $T _1$ plasmid transforms cells of plants.


Correct Option: D
Explanation:

In plant genetic engineering, the Ti plasmid can be used to carry foreign genes into plant cells. The Ti plasmid is the disease-causing agent of the soil-borne bacteria Agrobacterium tumefaciens. The Ti plasmid was then used to transform petunia cells in culture. So, the correct option is "D" (T1 plasmid transforms cells of plants).

Ti-plasmid used in genetic engineering has been modified by

  1. Adding tumour forming genes

  2. Deleting tumour forming genes

  3. Adding genes for endonucleases

  4. Deleting genes for endonucleases


Correct Option: B
Explanation:
The correct option is B.
Ti plasmid that is tumer-inducing plasmid refers to the plasmid of Agrobacterium tumefaciens. Role of Ti plasmid in biotechnology is that it can be modified into a cloning vector by removing the genes responsible for pathogenicity.

T$ _i$ plasmid transforms cells of

  1. Animals

  2. Plants

  3. Bacteria

  4. Fungi


Correct Option: B

In genetic engineering which is used for transfer of genes from one cell to another

  1. Vector

  2. Probe

  3. Plasmid

  4. Virus


Correct Option: A

Natural genetic engineer is

  1. Pseudomonas putida

  2. Agrobacterium tumefaciens

  3. Escherichia coli

  4. Bacillus sublilis


Correct Option: B

$T _1$ plasmid is used for making transgenic plants. It is obtained from

  1. Azotobacter

  2. Agrobacterium tumefaciens

  3. Rhizobium in leguminous root

  4. Yeast


Correct Option: B

Plasmids are suitable vectors for gene cloning because they are

  1. Small circular DNA molecules with their own origin of replication site

  2. Small circular DNA molecules which can integrate with host chromosomal DNA

  3. Having antibiotic genes

  4. Able to shuttle between prokaryotic and eukaryotic cells


Correct Option: A

Plasmids are used as vectors in genetic engineering because of their

  1. Resistance to antibiotics

  2. Resistance to restriction enzymes

  3. Ability to carry foreign genes

  4. Ability to cause infection in host


Correct Option: C

Insect tolerant gene from Bacillus thuringiensis is introduced using $T 1$ plasmid of _______.

  1. Escherichia coli

  2. Agrobacterium tumefaciens

  3. Haemophilus influenzae

  4. Arabidopsis thaliana


Correct Option: B

Vector for T-DNA is

  1. Salmonella typhimurium

  2. Thermits aquaticus

  3. Agrobacterium tumefaciens

  4. Escherichia coli

  5. Bacillus thuringiensis


Correct Option: C

Which of the following is correctly matched?

  1. Agrobacterium tumefaciens- Tumour

  2. pBR 322- Enzyme

  3. Thermus aquaticus- Bt gene

  4. Ligase- Molecular scissors

  5. Hind II- Plasmid vector


Correct Option: A

Pathogenic entity that is responsible for the production of tumour in most dicot plants is

  1. Retrovirus

  2. Bacteriophage

  3. Ti plasmid

  4. Vector


Correct Option: C
Explanation:

Agrobacterium is a genus of Gram-negative bacteria that causes tumors in plants. Agrobacterium tumefaciens is the most commonly studied species in this genus. It contains "Ti" plasmid or tumor inducing plasmid, that causes  crown-gall disease in plants. The disease is characterised by a tumour-like growth or gall on the infected plant, often at the junction between the root and the shoot. Tumors are incited by the conjugative transfer of a DNA segment (T-DNA) from the bacterial tumour-inducing (Ti) plasmid to the plant cell naturally when the bacterium infects the plant through  a wound.


So, the correct answer is 'Ti plasmid'

Diseases like malaria and filariasis are transmitted through insect vectors .Vectors can be controlled by introducing fish

  1. Labeo

  2. Gambusia

  3. Hilsa

  4. Catla


Correct Option: B

Consider the following statements:
1. AgrobacteriMm tMmejaciens is a soil bacterium.
2. AgrobacteriMm tumefaciens was the first vector used for introduction offoreign DNA in plant cells.
Which of the statements given above is/are correct?

  1. 1 only

  2. 2 only

  3. Both 1 and 2

  4. Neither 1 nor 2


Correct Option: A

pBR $322$ which is frequently used as a vector for cloning gene in E.coli is?

  1. An original bacterial plasmid

  2. A modified bacterial plasmid

  3. A viral genome

  4. A transposon


Correct Option: A

Which one is a cloning plasmid and not an expression plasmid?

  1. pBAD-18-Cam

  2. pBCSK

  3. pUC 18

  4. pET


Correct Option: C

Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines?

  1. Hepatitis B virus vaccine

  2. Herpes virus vaccine

  3. Salk's polio vaccine

  4. Both (A) and (B)


Correct Option: D
Explanation:
Second generation vaccines are the vaccines which are prepared by recombinant DNA technique/ genetic engineering, e.g., hepatitis B virus vaccine, herpes virus vaccine and pneumonia vaccine. The yeast-derived recombinant hepatitis B virus vaccine is the first commercially available human vaccine produced by the genetic engineering technology. Hepatitis B vaccine is produced from transgenic yeast by recombinant DNA technology.
So, the correct answer is 'Both (A) and (B)'.

Golden rice is _____________________.

  1. A variety of rice grown along the yellow river in China

  2. Long stored rice having yellow cocur tint

  3. A transgenic rice having gene for $\beta$-carotene

  4. Wild variety of rice with yellow colored grains


Correct Option: C
Explanation:

Golden rice is a transgenic variety of rice (Oryza Sativa) which contains good quantities of $\beta$-carotene ( provitamin A- inactive state of vitamin A). $\beta$-carotene is a principle source of vitamin A. Since the grains (seeds) of the rice are yellow in colour due to $\beta$-carotene, the rice is commonly called golden rice. 

The transgenic plant 'Flavr Savr' tomato carries an artificial gene for

  1. Delayed ripening process

  2. Longer shelf life

  3. Enhanced flavour

  4. All of these.


Correct Option: D
Explanation:

Flavr Savr is a genetically modified tomato  for human consumption. The transgenic plant 'Flavr Savr' tomato carries an artificial gene for slowing down the ripening process of the tomato and thus prevent it from softening and retains its natural colour , flavour and its shelf life. 

So, the correct option is 'Option D' .

Match column I containing transgenic organisms with their specific characteristics in column II and select the correct answer from the given codes.
Column I                   Column II
A. Golden rice        (i) Protein-enriched milk
B. Bt cotton            (ii) Increased shelf life
C. Flavr Savr          (iii) Enriched with vitamin A
D. Rosie cow         (iv) High yield and pest resistant

  1. A-(iii), B-(iv), C-(ii), D-(i)

  2. A-(iii), B-(ii), C-(iv), D-(i)

  3. A-(ii), B-(iv), D-(iii), D-(i)

  4. A-(i_, B-(iv), C-(ii), D-(iii)


Correct Option: A
Explanation:
Column I                   Column II
A. Golden rice        (iii) Enriched with vitamin A ( It is the improved variety of rice enriched with vitamin A).
B. Bt cotton             (iv) High yield and pest resistant ( Bt toxin are the toxins that kills harmful pests and responsible for high yield) .
C. Flavr Savr            (ii) Increased shelf life ( It is the improved variety of tomato that has long shelf life and causing them to soften ).
D. Rosie cow            (i) Protein-enriched milk ( Rosie is the first transgenic cow produced human protein rich milk i.e; 2.4 g per litres containing alpha-lactalbumin) .

So, the correct option is 'Option A' . 

Transgenic plants are the ones

  1. Grown on artificial medium after hybridisation in the field

  2. Produced after protoplast fusion in artificial medium

  3. Produced by somatic embryo on artificial medium

  4. Generated by introducing foreign DNA into a cell and regenerating plant from that cell


Correct Option: D

In which variety of rice a gene synthesizing vitamin A (Beta Carotene) has been introduced? 

  1. Jaya

  2. Golden Rice

  3. Ratna

  4. lndrayani


Correct Option: B
Explanation:

Golden rice is a variety of rice (Oryza sativa) produced through genetic engineering to biosynthesize beta-carotene, a precursor of vitamin A, in the edible parts of rice.

Golden/transgenic Rice is rich in ____________.

  1. Vitamin A

  2. Vitamin B

  3. Vitamin C

  4. Vitamin D


Correct Option: A

The resistant variety of rice to bacterial blight is

  1. Jaya

  2. TMK-6

  3. IR-20

  4. IR-8


Correct Option: D
Explanation:

  • Xanthomonas campestris causes bacterial blight of rice which is one of the most important diseases of rice in most of the rice-growing countries. Bacterial blight of rice has high epidemic potential and is destructive to high-yielding cultivars in both temperate and tropical regions especially in Asia.
  • IR8 is a high-yielding semi-dwarf rice variety developed by the International Rice Research Institute (IRRI) in the early 1960s. It is the resistant variety of rice to bacterial blight.
So, the correct answer is 'IR-8'

Hybrid of potato and tomato is

  1. Tomato

  2. Pomato

  3. Potamato

  4. None of the above


Correct Option: B

Transgenic plants are developed by

  1. Introducing foreign genes

  2. Introducing gene mutations

  3. Deleting certain chromosome parts

  4. Stopping spindle formation


Correct Option: A

In an experiment the gene of bioluminescence from firefly has been successfully transferred into a plant as a result of which it started to glow. In the terminology of the molecular genetics, the plant is called ___________.

  1. Firefly plant

  2. Hybrid plant

  3. Transgenic plant

  4. Bioluminescent plant


Correct Option: C
Explanation:

Transgenic plants are plants that have been genetically engineered, a breeding approach that uses recombinant DNA techniques to create plants with new characteristics. They are identified as a class of genetically modified organism (GMO). In this case, firefly's gene is transferred to the plant, hence the plant is called transgenic plant.


So, the correct answer is 'Transgenic plant'.

Give example of a GM crop that can tolerate abiotic stress. 

  1. Transgenic tomato

  2. Bt-Brinjal

  3. Golden rice

  4. Pomato


Correct Option: A
Explanation:

Transgenic tomato has been genetically engineered to have resistance to abiotic stress.

By Brinjal has pest resistance.
Golden rice is genetically engineered to have higher nutritional value.
Pomato is grafted from shoot of tomato plants and root of potato plant to obtain two products from the same plant.
So the correct answer is ' Transgenic tomato'.

Which of the following is a mismatch regarding the transgenic plant and its application?

  1. Golden rice $\Rightarrow$ Vitamin A enriched rice

  2. Flavr savr tomato $\Rightarrow$ Delay ripening

  3. Tobacco $\Rightarrow$ Herbicide resistant

  4. Bt corn $\Rightarrow$ Resistant to nematode


Correct Option: C
Explanation:

In Tobacco because of  biotechnology, transgenic plants produced which have low nicotine in their leaves. 

Transgenic plants have been shown to express the genes of
I) Insulin
II) Interferons
III) Human growth hormones
IV) Antibodies
V) Antibiotics.

  1. I, II, III, IV, V

  2. Except III, IV

  3. Except IV

  4. I, II, III only


Correct Option: A

RNA i us used to control pests on which plant?

  1. Tobacco

  2. Mango

  3. Potato

  4. Poppy


Correct Option: A

An improved variety of transgenic basmati rice?

  1. Does not require chemical fertilizers and growth hormones

  2. Gives high yield and is rich in vitamin A

  3. Is completely resistant to all insect pests and disease of paddy

  4. Gives high yield but has no characteristic aroma


Correct Option: A

Transgenic plants receive genes from which of the following organisms?

  1. Insects

  2. Bacteria

  3. Virus

  4. All of the above


Correct Option: D
Explanation:

There are several methods for introducing genes into plants, including:

  •     Infecting plant cells with plasmids as vectors carrying the desired gene.
  •     Shooting microscopic pellets containing the gene directly into the cell.
In contrast to animals, there is no real distinction between somatic cells and germline cells. Somatic tissues of plants, e.g., root cells grown in culture
  •     can be transformed in the laboratory with the desired gene;
  •     grown into mature plants with flowers.
If all goes well, the transgene will be incorporated into the pollen and eggs and passed on to the next generation.
In this respect, it is easier to produce transgenic plants than transgenic animals.
 Progress is being made on several fronts to introduce new traits into plants using recombinant DNA technology.
The genetic manipulation of plants has been going on since the dawn of agriculture, but until recently this has required the slow and tedious process of cross-breeding varieties. Genetic engineering promises to speed the process and broaden the scope of what can be done.

Transgenic plants of tomato having resistance to hornworm larvae are those which have

  1. Foreign resistant gene

  2. Mutated resistant gene

  3. Synthetic resistant gene

  4. Superimposed resistant gene


Correct Option: A
Explanation:

Transgenic tomato plants expressing a truncated insect control protein gene from Bacillus thuringiensis were  protected from attack by tobacco hornworm, tobacco budworm and tomato fruit worm larvae. Filed tests involving transgenic tomato lines challenged with tobacco hornworm, tomato fruit worm and tomato pin worm were conducted for two years. These transgenic plants suffered only minor feeding damage from tobacco hornworm, while the untransformed controls were completely defoliated. The foreign gene present had made this possible.

A tumour inducing plasmid widely used in the production of transgenic plant is that of

  1. Escherichia coli

  2. Bacillus thuringiensis

  3. Staphylococcus aureus

  4. Agrobacterium tumefaciens


Correct Option: D
Explanation:

A tumor (crown gall) inducing plasmid widely used in the production of transgenic plants is Agrobacterium tumefaciensBacillus thuringiensis is used for the production of disease-resistant plants. Other 2 are disease-causing bacteria.

Transgenic plant of tobacco was mainly used to produce

  1. Antibody

  2. Enhance the tolerance to water stress

  3. Enhance flowering

  4. Both A and B


Correct Option: D
Explanation:

The tobacco plant was genetically modified by introducing a gene to produce antibodies. The plant was introduced with the gene for producing antibodies against the rabies virus which are similar to the antibodies produced in the humans. The antibodies from the plant can be purified and used as injections or vaccines. These vaccines help in providing immunity against the rabies virus. 

The tobacco plants can also be modified to survive under the conditions of stress. The plants can bear extreme conditions like water stress, salinity and heat stress. 
Thus, the correct answer is option D. 

Glycerol-1-phosphate acyltransferase enzyme was extracted by

  1. Glycine max

  2. Arabidopsis

  3. Beta vulgaris

  4. Secale cereale


Correct Option: B

Which of the following attributes can be improved in ornamental plants?

  1. Regulation of flowering

  2. Weed control

  3. Promotion of vegetative growth

  4. All of the above


Correct Option: D
Explanation:
Ornamental plants are known for the decorative purposes in gardens and landscape design projects, as houseplants, for cut flowers and specimen display.
The regulation of flower can be improved in ornamental plants through physical alteration of day length or modification of hormone biosynthesis and metabolism.
Weeds can be controlled by resistance to a broad spectrum herbicide like glyphosate which would allow the protection of young plants from weed seedlings in new areas and prevent damage from spot- spraying of weeds in established beds.
The vegetative growth of the ornamental plants can be promoted by using various growth regulators.
So, the correct answer is option D.

Salt tolerant transgenic has been developed for

  1. Brinjal

  2. Grape

  3. Potato

  4. Tomato


Correct Option: D
Explanation:
A genetically engineered tomato plant that thrives in salty irrigation water and may hold the key to one of agriculture's greatest dilemmas has been developed by plant biologists at the University of California, Davis, and the University of Toronto.
As the first truly salt-tolerant crop, these tomatoes offer hope that other crops can also be genetically modified for planting in many areas of the world that have salty irrigation water and salt-damaged soils.
Since environmental stress due to salinity is one of the most serious factors limiting the productivity of crops, this innovation will have significant implications for agriculture worldwide.

Central Rice research institute (CRRI) is located in

  1. Coimbatore

  2. Cuttack

  3. Shimla

  4. Trivendrum


Correct Option: B
Explanation:

CRRI develops new rice varieties and rice crop management techniques that help rice farmers improve the yield and quality of their rice in an environmentally sustainable way. The Institute works with its public and private sector partners in national agricultural research and extension systems in major rice-growing countries to do research, training, and knowledge transfer. It is situated in Cuttack, India. Thus, option B is correct.

Which among the following is a tumour inducing plasmid widely used in the production of transgenic plants?

  1. Escherichia coli

  2. Bacillus thuringiensis

  3. Staphylococcus aureus

  4. Agrobacterium tumefaciens


Correct Option: D
Explanation:

Plant transformation mediated by the soil-plant pathogen Agrobacterium tumefaciens has become the most used method for plant transformation. A. tumefaciens naturally infects the wound sites in dicotyledonous plant causing the formation of the crown gall tumours. A. tumefaciensis is capable of transferring a particular DNA segment (T-DNA) of the tumour-inducing (Ti) plasmid into the nucleus of infected cells where it is subsequently integrated into the host genome and transcribed, causing the crown gall disease.

'Himgiri' developed by hybridisation and selection for disease resistance against rust pathogens is a variety of _________.

  1. Wheat

  2. Chilli

  3. Maize

  4. Sugarcane


Correct Option: A
Explanation:
Himgiri is a hybrid species of wheat which is resistant to crop diseases like leaf rust, hill bunt and many more, that's why this particular species is very popular in the Indian agriculture productivity sector.
So, the correct answer is 'Wheat'

Flavr Savr is a genetically modified ____________.

  1. Cotton

  2. Rice

  3. Tomato

  4. Potato


Correct Option: C

Increased flavourful shelf life of Tomato has been achieved by ______________.

  1. Developing better storage technique

  2. Reducing activity of enzyme polygalactourinase

  3. Promoting activity of enzyme polygalacturonase

  4. Enhancing epidermal growth factor


Correct Option: B

Vitamin A rich transgenic plant is ___________.

  1. Flavr Savr Tomato

  2. Golden Rice

  3. Bt Cotton

  4. Vaccinated Potato


Correct Option: B

Salt tolerant transgenic has been developed for ______________.

  1. Brinjal

  2. Potato

  3. Tomato

  4. Grape


Correct Option: C

Golden rice is a variety rich in

  1. $\beta$-Carotene and ferritin

  2. Lysine

  3. Vitamin C

  4. Biotin


Correct Option: A

Flavr Savr variety of tomato is _____________.

  1. High yielding hybrid variety

  2. High yielding new variety

  3. Transgenic variety

  4. Polyploid variety


Correct Option: C

An improved variety of transgenic basmati rice _________________.

  1. Gives high yield and is rich in vitamin A

  2. Is completely resistant to all insect pests and diseases of paddy

  3. Gives high yield but has no characteristic aroma

  4. Does not require chemical fertilizers and growth hormones


Correct Option: A

Transgenic plants have _________________.

  1. No gene

  2. Genes in transposition

  3. Genes have no function to perform

  4. Genes of other organism


Correct Option: D

Golden Rice will help in ___________.

  1. Producing petrol-like fuel

  2. Pest resistance

  3. Herbicide tolerance

  4. Alleviation of vitamin A deficiency


Correct Option: D

Which of the following is obtained from genetic engineering?

  1. Glucose

  2. Haemoglobin

  3. Golden Rice

  4. None of the above


Correct Option: C

The problem of blindness in poor countries can be overcome by using _________,

  1. Golden rice

  2. Transgenic maize

  3. Transgenic tomato

  4. Bt Brinjal


Correct Option: A

A regulatory body working under MoEF for the release of transgenic crop is ____________.

  1. NBPGR

  2. NSC

  3. NIPGR

  4. GEAC


Correct Option: D

First transgenic plant released for commercial use was _____________.

  1. Bt cotton

  2. Tobacco

  3. Golden rice

  4. Solan gola


Correct Option: B

Flavr Savr Tomato has increased ____________.

  1. Productivity

  2. Vigour

  3. Shelf life

  4. Flowering period


Correct Option: C

Golden colour of Rice is due to occurrence of _____________.

  1. Vitamin A

  2. Vitamin C

  3. Vitamin K

  4. Vitamin B$ _6$


Correct Option: A

Tobacco plants resistant to nematodes have been developed by introduction of DNA that produces _____________.

  1. Both sense and anti sense RNA

  2. An antifeedent

  3. A toxic protein

  4. A particular hormone


Correct Option: A

Genetically modified crops can be produced by ___________.

  1. Recombinant DNA technology

  2. Somatic hybridisation

  3. Cross breeding

  4. Micropropagation


Correct Option: A

The introduction of t-DNA into plants involves

  1. Infection of the plant by Agrobacterium tumefaciens

  2. Altering the pH of the soil, then heat shocking the plants

  3. Exposing the plants to cold for a brief period

  4. Allowing the plant roots to stand in water


Correct Option: A

Strategy used to prevent nematode infection of tobacco roots is _______________.

  1. Use of agrochemicals

  2. Bt toxin gene

  3. Gene mutation

  4. RNA interference


Correct Option: D

Which one of the following statements is incorrect?

  1. Tobacco is obtained from the seeds of Nicociana tobacco

  2. Rubber is obtained from the latex of some trees

  3. Fruits of Embolic officials are eaten raw and in pickled from

  4. Tectona grandis yields good timber


Correct Option: A

Golden rice is a transgenic crop with a trait for ________________.

  1. Pest resistance

  2. High vitamin a content

  3. Drought resistance

  4. High protein content


Correct Option: A

Consider the following statements:
In a ripe tomato, as compared to an unripe tomato, the amount of.
a. Vitamins is higher
b. Carbohydrates is less
c. Minerals is less
d. Proteins is higher
Of these statements.

  1. $1, 3$ and $4$ are correct

  2. $1, 2$ and $4$ are correct

  3. $2, 3$ and $4$ are correct

  4. $1, 2$ and $3$ are correct


Correct Option: A

Introduction of food plants developed by genetic engineering is not desirable because -

  1. Economy of developing countries may suffer

  2. These products are less tasty as compared to the already existing products

  3. This method is costly

  4. There is danger of coming viruses, allergens and toxins with introduced crop


Correct Option: A
Explanation:
It is difficult for developing countries to keep up and maintain genetically engineered crops. Therefore, the introduction of food plants by genetic engineering is not desirable for developing countries.

So, the correct answer is 'Economy of developing countries may suffer'.

Trifolirhizin, a phytoalexin has been reported from.

  1. Soybean roots

  2. Red clover roots

  3. Bean pods

  4. Pea pods


Correct Option: A

Transgenic plants are the ones -

  1. Grown in artificial medium after hybridization in the field

  2. Produced by a somatic embryo in artificial medium

  3. Generated by introducing foreign DNA in to a cell and regenerating a plant from that cell

  4. Produced after protoplast fusion in artificial medium


Correct Option: C

In tomato, genotype $aabbcc$ produces $100gm$ tomatoes and $AABBCC$ produces $160gm$ tomatoes what is the contribution of each polygenes in the production of tomatoes?

  1. $10gm$

  2. $20gm$

  3. $30gm$

  4. $40gm$


Correct Option: A

Biolistices (gene - gun) is suitable for 

  1. disarming pathogen vectors

  2. transformation of plant cells

  3. constructing recombinant $DNA$ by joining with vectors

  4. $DNA$ finger printing


Correct Option: B
Explanation:
In genetic engineering, a gene gun or a biolistic particle delivery system, originally designed for plant transformation, is a device for delivering exogenous DNA (transgenes) to cells. The payload is an elemental particle of a heavy metal coated with DNA (typically plasmid DNA). This technique is often simply referred to as biolistics.

This device is able to transform almost any type of cell, including plants, and is not limited to transformation of the nucleus; it can also transform organelles, including plastids.

So the correct option is 'transformation of plant cells'.

First transgenic plant released for commercial use was

  1. $Bt-$cotton

  2. tobacco

  3. golden rice

  4. solan gola


Correct Option: B
Explanation:
A nematode affected the roots of the tobacco plant on the cost of great production and yield. Strategies adopted to prevent this infection that was based on the process of RNA interference. RNA interference is the phenomena of inhibiting the activity of a gene through production sense and antisense RNA. The method involves a specific and RNA silencing. The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. Transgenic plant thus gets protected from the parasite.
So, the correct option is 'tobacco'

(A) Source of sense &- Transposon 
      Antisense RNA gene
(B) Host of sense &- Tobacco 
      Antisense  RNA gene
(C)   Site of iRNA  - Tobacco 
        Production
(D) Site of pre RISC  - Meloidegyne     
      formation
(E) Site of pre RISC to RISC &- Meloidegyne     
      conversion
(F) Destruction  of- Gastric tract of      
      functional m-RNA    Meloidegyne      gene
In Nematode resistant plant formation, among
given combinations which are incorrect

  1. D & E

  2. D & F

  3. C & E

  4. A & F


Correct Option: A
Explanation:
  • Transgenic plants are those plants whose genome is altered by the addition of one or more transgenes. Tobacco plant is the most common plant for expressing the transgenes. As the genome of tobacco plant is completely studied. The transgenic plants are transcribed in such a way that they develop tolerance against stress, salt, herbicide and also for vaccine productions. There are several transgenes which are inserted in tobacco plant like cry2Aa gene which gives resistance against any worms.
  • The Meloidogyne does not perform the conversion of sites like pre-RISC to RISC. The Meloidogyne gene is obtained by the species Meloidegyne incognita which are mainly responsible for causing root knot in plants.
  • Therefore the correct answer is option "A".

Which one the following is a transgenic crop?

  1. Brinjal

  2. Potato

  3. Grape

  4. Pomato


Correct Option: D
Explanation:

A transgenic crop plant contains a gene or genes which have been artificially inserted instead of the plant acquiring them through pollination. The inserted gene sequence (known as the transgene) may come from another unrelated plant or from a completely different species. In many cases, the inserted DNA has to be modified slightly in order to correctly and efficiently express in the host organism. The pomato is a chimera produced by grafting a tomato plant and a potato plant, both of which are members of the Solanaceae family. Pomato plants make food production more efficient, as they maximize the amount of crops that can be produced on a piece of land. In addition, grafting can improve resistance to bacteria, viruses and fungi, attract a more diverse group of pollinators and provide a sturdy trunk for delicate ornamental plants.

Transgenic plants are

  1. Produced after protoplast fusion in artificial medium

  2. Grown in artificial medium after hybridization in the field

  3. Produced by a somatic embryo in artificial medium

  4. Generated by introducing foreign DNA into a cell and regenerating a plant from that cell.


Correct Option: D
Explanation:

Transgenic plants are generated with the help of recombinant DNA technology (RDT). Recombinant DNA is a DNA that constitute genes or parts of genes from two or more organisms, usually of different species (or is specifically the gene of interest). Thus, transgenic plants are generated by incorporating foreign DNA into the target organism, and it helps in the formation of the transgenic plant. Transgenic plants are also known as the GMO as the process of genetic engineering and RDT plays an important role in their development.

The genetically modified crops introduced in India are

  1. Cotton

  2. Mustard

  3. Wild plants

  4. Both A and B


Correct Option: D
Explanation:

Genetically modified crops (GMCs, GM crops, or biotech crops) are plants used in agriculture, the DNA of which has been modified using genetic engineering techniques. In most cases, the aim is to introduce a new trait to the plant which does not occur naturally in the species. For example, in food crops include resistance to certain pests, diseases or environmental conditions, reduction of spoilage, resistance to chemical treatments (e.g., resistance to a herbicide) or improving the nutrient profile of the crop. GM mustard is being developed to increase the productivity of mustard to meet the ever-growing demand for edible oil in the country. Bt cotton is a genetically modified variety of cotton producing an insecticide. It is produced by Monsanto. It is supplied in India's Maharashtra state.

Therefore, the correct answer is option D.

Transgenic plants are produced by using Ti plasmid from the

  1. Agrobacterium tumefaciens

  2. E.coli

  3. Bacteriophage

  4. Agrobacterium varians


Correct Option: A
Explanation:

A Ti or tumour inducing plasmid is a circular plasmid that is a part of the genetic equipment that Agrobacterium tumefaciens and Agrobacterium rhizogenes use to transduce its genetic material to plants. The Ti plasmid is lost when Agrobacterium is grown above 28$^o$C. Such, cured bacteria do not induce crown galls, i.e., they become avirulent.

A transgenic food crop which may help solving the problem as night blindness in developing countries

  1. Flavr Savr tomatoes

  2. Starlink maize

  3. Bt soybean

  4. Golden rice


Correct Option: D
Explanation:

Night blindness is the inability to see normally in subdued light. It is usually a result of vitamin A deficiency. Golden rice is such a biofortified crop. Golden rice grains are easily recognisable by their yellow to orange colour. The stronger the colour the more carotene. Rice produces provitamin A (carotene) in the leaves but not in the grain, where the biosynthetic pathway is turned off during plant development. In golden rice, two genes have been inserted into the rice genome by genetic engineering, to restart the carotenoid biosynthetic pathway leading to the production and accumulation of carotene in the grains. Both genes are naturally involved in carotene biosynthesis. 

Therefore, the correct answer is option D.

The improved trait in golden rice is

  1. High glysine content

  2. High protein content

  3. Insect resistance

  4. High vitamin A content


Correct Option: D
Explanation:

Golden rice is a variety of rice (Oryza sativa) produced through genetic engineering to biosynthesize beta-carotene, a precursor of vitamin A, in the edible parts of rice. The research was conducted with the goal of producing a fortified food to be grown and consumed in areas with a shortage of dietary vitamin A, a deficiency which is estimated to kill 670,000 children under the age of 5 each year.
Golden rice differs from its parental strain by the addition of three beta-carotene biosynthesis genes. 

Golden rice is enriched in

  1. Vit C

  2. Vit A

  3. Vit D

  4. All


Correct Option: B
Explanation:

Golden rice is yellow coloured enriched with pro-vitamin A (by introducing the genes).

Transgenic plants are

  1. Produced by a somatic embryo in an artificial medium.

  2. Generated by introducing foreign DNA into a cell and regenerating a plant form.

  3. Produced after protoplast fusion in an artificial medium.

  4. Grown in an artificial medium after hybridization in the field.


Correct Option: B
Explanation:

A transgenic plant is a plant, with an insertion of DNA from another organism. This technology can be used as a tool for researchers to insert useful genes as a means of improving crops.

Thus, the correct answer is option B.

Golden rice is a promising transgenic crop. When released for cultivation, it will help in

  1. Herbicide tolerance

  2. Producing a petrol-like fuel fro m rice

  3. Alleviation of vitamin A deficie ncy

  4. Pest resistance


Correct Option: C

Which of the following is an improved variety of wheat crop?

  1. Roja-64 A

  2. Sonalika

  3. Sonara-64

  4. All of these


Correct Option: D
Explanation:
Roja-64 A, Sonalika, Sonara-64 all are improved variety of wheat crop.
So, the correct option is 'All of these'

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?

  1. Falver Savr' tomato

  2. Canolla

  3. Golden rice

  4. Bt-Brinjal


Correct Option: C
Explanation:

Golden rice is a variety of Oryza sativa rice produced through genetic to biosynthesize beta-carotene, a precursor of vitamin-A in the edible part of rice. The research that led to golden rice was conducted with the goal of helping children who suffer from vitamin A.

So, the correct option is 'Golden rice'.

Golden rice is rich in

  1. vitamin B

  2. vitamin A

  3. both (a) and (b)

  4. none of these


Correct Option: B
Explanation:

Golden rice is a variety of rice produced through genetic engineering to biosynthesize beta-carotene, a precursor of vitamin A, in the edible parts of rice.

So, the correct option is vitamin A.

Basic principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus to.

  1. somatic cell

  2. vegetative cell

  3. germ cell

  4. body cell


Correct Option: C
Explanation:

The principle of developing transgenic plants and animals is to introduce the gene of interest into the nucleus to germ cell.

So, the correct option is 'Germ cell'.

Which one of the following bacteria is used for production of transgenic plants?

  1. Escherichia coli

  2. Bacillus thuringiensis

  3. Staphylococcus aureus

  4. Agrobacterium tumefaciens


Correct Option: B
Explanation:

Bt corn varieties, for example, contain a gene from a bacterium found in the soil that causes the transgenic corn to produce an insecticidal protein.

So, the correct option is 'Bacillus thuringiensis'.

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of.

  1. Vitamin-B

  2. Vitamin-C

  3. Omega-$3$

  4. Vitamin-A


Correct Option: D
Explanation:

golden rice is a genetically modified crop plant where the incorporated gene. Golden rice is a variety of rice produced through genetic engineering to biosynthesize beta-carotene, a precursor of vitamin A, in the edible parts of rice.

So, the correct option is 'Vitamin A'.

RNA interference which is employed in making tobacco plant resistant to Meloidegyne incognita is essentially involved in

  1. preventing the process of replication of DNA

  2. preventing the process of translation of mRNA

  3. preventing the process of splicing of hnRNA

  4. preventing the process of transcription


Correct Option: B
Explanation:

RNA interference is a gene-silencing process that blocks the expression of genes in the parasite when it enters the host's body.RNA is a method adopted to prevent infestation of roots of tobacco plants by a nematode Meloidogyne incognita. In RNA, a complementary RNA binds to mRNA to form a ds RNA that cannot translate and hence, its expression is blocked.

So, the correct option is 'Preventing the process of translation of mRNA'.

Which part of the tobacco plant is infected by Meloidogyne incognita?

  1. Leaf

  2. Stem

  3. Root

  4. Flower


Correct Option: C
Explanation:

Roots of the tobacco plants are infected by Meloidogyne incognita. RNA I method is used to develop resistance in these plants against infection.

So, the correct option is 'Root'.

Golden rice is a transgenic crop of the future with the following improved trail

  1. insect resistance

  2. high lysine (essential amino acid) content

  3. high protein content

  4. high vitamin-A content


Correct Option: D
- Hide questions