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Chemiosmotic hypothesis - class-X

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Who am I?

  1. I am ATP producing factory

  2. I am single layered, but maintain cellular osmotic pressure

  3. I support the cell, but I am not cell wall I have a body resembling net

  4. I am chemical factory of the cell

  5. Leaves are green because of me


Correct Option: A

How many ATP molecules will be produced when one molecule of 3-phosphosphoglyceric acid is completely oxidised : 

  1. 16

  2. 32

  3. 64

  4. 48


Correct Option: A

How many ATP molecules are produced after complete oxidation of one molecule of fructose-1, 6-bis-phosphate ?

  1. 40

  2. 32

  3. 38

  4. 44


Correct Option: A

During photosynthesis 

  1. $O _{2}$ evolved comes from $CO _{2}$

  2. ATP is formed

  3. ATP is not formed

  4. Water is required as medium but it does not take part in photosynthesis


Correct Option: B
Explanation:

Photosynthesis is an anabolic process, which helps in forming ATP, where it is called as energy currency. During the light reaction photophosphorylation takes place producing ATP and $NADPH _2$ which is used as energy source for dark reaction.

ATP synthesis proposed by Peter Mitchell is 

  1. Phosphorylation

  2. Photophosphorylation

  3. Oxidative phosphorylation

  4. Chemiosmotic synthesis


Correct Option: D
Explanation:

Peter Mitchell proposed a theory to explain the production of ATP which is 'Mitchell's chemiosmotic coupling hypothesis'. According to this theory:

  1. Inner membrane of mitochondria acts as a transducer of energy and converts energy to chemical energy of ATP along the electrochemical gradient.
  2. Inner mitochondrial membrane is impermeable to NADH, ATP, H+, OH-.
  3. ATP synthesis and electron transport are coupled with proton gradient.
So the answer is 'Chemiosmotic synthesis'.

Energy required to synthesise ATP from ADP and inorganic phosphate is

  1. 2500 cal

  2. 7600 cal

  3. 12000 cal

  4. 20000 cal


Correct Option: B

ATP is injected in cyanide poisoning because it is

  1. Necessary for cellular functions

  2. Necessary for $Na^+$ - $K^+$ pump

  3. $Na^+$ - $K^+$ pump operates at the cell membranes

  4. ATP breaks down cyanide


Correct Option: A

Chemiosmotic hypothesis given by Peter Mitchell proposes the mechanism of

  1. Synthesis of ATP.

  2. Synthesis of FADH$ _2$.

  3. Synthesis of NADH.

  4. Synthesis of NADPH.


Correct Option: A
Explanation:

Peter Mitchell proposed a hypothesis known as the chemiosmotic hypothesis that an electrochemical concentration gradient of protons across a membrane could be utilized to make ATP. He linked this process to osmosis, the diffusion of water across a membrane, this is the reason it is called chemiosmosis.

So the correct option is 'Synthesis of ATP'.

Light reaction in stroma lamellae of chloroplast results in the production of

  1. ATP

  2. NADPH$ _2$

  3. ATP + NADPH$ _2$

  4. O$ _2$


Correct Option: C
Explanation:

A) Along with ATP, ${ NADPH } _{ 2 }$ is also produced.
B) Along with ${ NADPH } _{ 2 }$, ATP is also produced.

C) The light reaction that takes place in the stroma lamellae is the non-cyclic phosphorylation and ATP and ${ NADPH } _{ 2 }$ are the products.
D) Oxygen is also liberated during the light reaction but is not the main product.
So the correct answer is 'ATP + ${ NADPH } _{ 2 }$'.

ATP does not provide phosphate in the reaction

  1. Glucose Glucose 6-P

  2. Fructose Fructose 6-P

  3. PGAL 1 : 3-diPGA

  4. PEPA Pyruvic acid


Correct Option: C

ATP synthetase of chloroplasts is similar to that of

  1. Mitochondria

  2. Peroxisomes

  3. Golgi bodies

  4. Microsomes.


Correct Option: A
Explanation:

Mitochondria and chloroplast both have:

  • A double membrane surrounding the organelles.
  • Purportedly prokaryotic origins according to the endosymbiotic theory which suggests that mitochondria and chloroplast were once prokaryotic bacteria engulfed by endocytosis in early eukaryotes.
  • Their own circular DNA which codes for certain enzymes required for the chemical reactions that take place in these organelles.
  • Their own 70S ribosomes made up of 50S and 30S subunits to translate proteins
  • The enzyme ATP synthase which utilizes the energy released from the movement of protons across it (proton-motive force) to phosphorylate ADP to ATP. (Thus, another similarity would be that they both produce ATP)
  • Electron transport chains, which are embedded in the inner mitochondrial membrane and thylakoid membrane in mitochondria and chloroplasts respectively.
So, the correct option is 'Mitochondria'.

ATP formation in chloroplast and mitochondrion is explained by

  1. Cholodny-Went model

  2. Chemi-osmotic theory of Mitchell

  3. Munch's mass flow theory

  4. Relay pump theory of Godlewski


Correct Option: B
Explanation:
A theory postulated by the biochemist Peter Mitchell in 1961 to describe ATP synthesis by way of a proton electrochemical coupling.
it is based on the proton gradient and transfer of an electron from the mitochondrial matrix in respiration or in the stroma with the photophosphorylation of photosystems forms ATP.
So the correct option is 'Chemi-osmotic theory of Mitchell'.

Chemiosmosis was first described by

  1. Boyer

  2. Walker

  3. Mitchell

  4. Meischer


Correct Option: C
Explanation:

The chemiosmotic hypothesis of ATP synthesis in chloroplasts is based on proton gradient. The chemiosmotic hypothesis was explained by P. Mitchell in 1961. He recieved noble prize for it in 1978. ATP synthesis is linked to the development of a proton gradient across the membrane of the thylakoid of chloroplast and proton accumulation is towards the inside of the membrane i.e. in the lumen. 

When cell converts light energy into chemical energy, which of the following reaction would take place?

  1. ADP + iP = ATP

  2. ATP - iP = ADP

  3. AMP + iP = ADP

  4. GDP + iP = GTP


Correct Option: A
Explanation:

Cells convert light energy is converted into chemical energy. During this process, a phosphate is added to adenosine diphosphate molecule to cause the formation of ATP.

So the correct option is 'ADP + iP = ATP'.

Oxidative phosphorylation refers to

  1. Anaerobic production of ATP

  2. The citric acid cycle production of ATP

  3. Production of ATP by chemiosmosis

  4. Alcoholic fermentation


Correct Option: C

ATP synthesis in cell requires

  1. $H^+$ gradient across the membrane

  2. $K^+$ gradient across the membrane

  3. $PO^{3-} _4$ gradient across the membrane

  4. $Ca^{2+}$ gradient across the membrane


Correct Option: A
Explanation:
A) A ${ H }^{ + }$ gradient or proton pump is developed in the lumen of the thylakoid which helps in ATP synthesis.
B) ${ K }^{ + }$ does not play a role in ATP synthesis.
C) ${ PO } _{ 4 }^{ 3- }$ does not play a role in ATP synthesis.
D) ${ Ca }^{ 2+ }$ is involved in the photolysis of water.
So the correct answer is '${ H }^{ + }$ gradient across the membrane'.

ATP was discovered by 

  1. Blackman

  2. Bowman

  3. Lipmann

  4. Karl Lohmann


Correct Option: D
Explanation:

ATP was discovered by Karl Lohman. ATP is the energy currency of our cell. It is formed as a result of phosphorylation.

The chemiosmotic coupling hypothesis of oxidative phospohorylation proposes that adenosine triphosphate is formed because

  1. A Proton gradient forms across the inner membrane

  2. There is a change in the permeability of the inner mitochondria membrane toward adenosine diphosphate

  3. High energy bonds are formed in mitochondrial proteins

  4. ADP is pumped out of the matrix into the intermembrane space


Correct Option: A

Chemiosmotic mechanism of ATP synthesis was proposed by 

  1. Warburg

  2. Dickens

  3. Krebs

  4. P. Mitchell


Correct Option: D
Explanation:

Chemiosmosis is the movement of ions across a selectively permeable membrane, down their electrochemical gradient. More specifically, it relates to the generation of ATP by the movement of hydrogen ions across a membrane during cellular respiration or photosynthesis. Peter D. Mitchell proposed the chemisomotic hypothesis in 1961. The theory suggests essentially that most ATP synthesis in respiring cells  comes from the electrochemical gradient across the inner membranes of mitochondria by using the energy of $NADH _2$ and $FADH _2$ formed from the breaking down of energy-rich molecules such as glucose.

Chemiosmotic theory of ATP synthesis in the chloroplasts and mitochondria is based on

  1. Membrane potential

  2. Accumulation of K ions

  3. Proton gradient

  4. Accumulation of Na ions


Correct Option: C

With reference to photosynthesis, what is the $CF _0$ - $CF _1$ complex?

  1. ATP synthetase

  2. Ferredoxin-NADP reductase

  3. Cytochrome $b6$/f complex

  4. RuBP carboxylase


Correct Option: A

ATP is known as energy currency of a cell. ATP is synthesized from ADP and Pi. The reaction is catalyzed by an enzyme called as ATP synthase. When the enzyme and substrate (ADP) are distant inside the cell, the reaction cannot take place spontaneously. Which of the following statements best explains the cause for the ATP synthase and ADP to interact?

  1. Electronegativity of ADP attracts ATP synthase

  2. ADP will be actively pumped to ATP synthase

  3. Random movements will bring ADP to ATP synthase

  4. ADP and ATP synthase always remain bound to oxysomes


Correct Option: A

How many ATP molecules could maximally be generated from one molecule of glucose, if the complete oxidation of one mole of glucose to $CO _2$ and $H _2O$ yields $686$kcal and the useful chemical energy available in the high energy phosphate bond of one mole of ATP is $12$kcal?

  1. $1$

  2. $2$

  3. $30$

  4. $57.1$


Correct Option: A

Calvin cycle has three stages.
$1$. Reduction during which carbohydrate is formed at the expense of photochemically made ATP and NaDPH.
$2$. Regeneration during which carbon dioxide acceptor $1, 5$-RuBP is formed.
$3$. Carboxylation during which $CO _2$ combines with $1, 5-$RuBP.
Identify the correct sequence.

  1. $3, 1, 2$

  2. $3, 2, 1$

  3. $1, 2, 3$

  4. $2, 1, 3$


Correct Option: A

A universal hydrogen acceptor in an electron transport system is ___________.

  1. ATP

  2. UDP

  3. NAD

  4. FMN


Correct Option: A

The idea that pH gradients could provide energy for ATP formation in chloroplasts and mitochondria was first proposed by ____________.

  1. Bassham

  2. Hill

  3. Mitchell

  4. Thompson


Correct Option: A

Which of the following step of photosynthesis is not directey involve in ATP synthesis or consumption?

  1. Corboxylation of dark Rection

  2. Reduction of dark reaction

  3. Cyclic photo phosphorylation of light reaction

  4. Regeneration of dark reaction


Correct Option: A

Direct gain of ATP from one mole of glucose during glycolysis or EMP pathaway ____________.

  1. 2 ATP

  2. 6 ATP

  3. 36 ATP

  4. 38 ATP


Correct Option: A

Photosynthetic oxygen producing cells differ from non-oxygen producing cells in that the former.

  1. Produce one ATP whereas the latter produce two

  2. Produce no NADPI-$1$ whereas the latter do

  3. Reduce ferredoxin whereas the latter do not

  4. Contain and use both photo systems I and II


Correct Option: A

Mitochondrial morphology is maintained by balancing the active absorption of water and active secretion by the mitochondrion. Hence mitochondria are likely to swell in the presence of.

  1. ATP

  2. Hypertonic solution

  3. Uncoupling agent

  4. Enzymes catalyst oxidative phosphorylation


Correct Option: A

How many ATP molecules could maximally be generated from on molecule of glucose, if the complete oxidation of one mole of glucose to $C{ O } _{ 2 }$ and $H _{ 2 }O$ yields 686 kcal and the useful chemical energy available in the high energy phosphate bond of one mole of ATP is 12 kcal ?

  1. $\displaystyle Two$

  2. $\displaystyle Thirty$

  3. $\displaystyle Fifty \ seven$

  4. $\displaystyle One$


Correct Option: A

How many ATP molecules could maximally be generated from one molecule of glucose, if the complete oxidation of one mole of glucose to $CO _{2}$ and $H _{2}O$ yields $686\ kcal$ and the useful chemical energy available in the high energy phosphate bond of one mole of ATP is $12\ kcal$?

  1. Two

  2. Thirty

  3. Fifty seven

  4. One


Correct Option: A

Irradiation of a mixture of $NH _3, CH _4, H _2$ and $H _2O$ vapour resulted in the formation of adenine. Such experimental evidence supports Oparin's hypothesis on the origin of life because adenine is found in.

  1. ATP

  2. RNA

  3. DNA

  4. ATP, RNA and DNA


Correct Option: A

The primary reaction in photosynthesis is photochemical and takes place in the chloroplast. It is accomplished by a chain of electron carriers with phosphorylation of ADP to ATP. The actual site for this reaction is?

  1. Intrathylakoid space

  2. Thylakoid membrane

  3. Chloroplast envelope

  4. Stoma


Correct Option: A

Chemiosmosis refers to the view that an ion flowing down its electrochemical gradient drives ATP synthesis. The ion in question is?

  1. Phosphate

  2. Sodium

  3. Iron

  4. Hydrogen


Correct Option: A

The energy-carrying compound in the living cell is ATP.

  1. True

  2. False


Correct Option: A
Explanation:

ATP captures chemical energy obtained from the breakdown of food molecules and its hydrolysis (ATP to ADP + Pi or ADP to AMP + Pi) releases energy that can be used to drive various energy-requiring processes in cells. So, the correct option is 'True'.

Choose the correct answers from the alternatives given .
Energy coupling of endergonic and exergonic reactions within cells

  1. Permits biological reactions to proceed at temperatures consistent with life

  2. Uses heat released by one reaction to fuel the other reaction

  3. Utilizes ATP to carry energy between the exergonic and endergonic reactions

  4. All of the above


Correct Option: D
Explanation:
Energy coupling is the use of an exergonic process to carry out an endergonic process. The energy released in an exergonic process is used to develop an endergonic process. The ATP is responsible for mediating the energy coupling of cells, it is the energy currency. It is used to boost many cellular reactions that require energy. This also provides the energy required for reactions without too much increase in temperature. 
So, the correct option is 'All of the above'.

Read the given statements and select the correct option. 
Statement 1 : In photosynthesis, during ATP synthesis, protons accumulate in the lumen of thylakoid.
Statement 2 : In respiration, during ATP synthesis, protons accumulate in the intermembrane space of mitochondria. 

  1. Both statement 1 and 2 are correct

  2. Statement 1 is correct but statement 2 is incorrect.

  3. Statement 1 is incorrect but statement 2 is correct

  4. Both statement 1 and 2 are incorrect


Correct Option: A
Explanation:

Photolysishappens in the thylakoids, producing H+ ions inside the thylakoid membrane., a gradient of hydrogen ions will form across the membrane, ATP synthase take the advantage of this gradient, by allowing hydrogen ions to leak out of the membrane capturing their energy and phosphorylating ADP into ATP, while another statement is correct for mitochondria.

So, the correct option is 'Both statement 1 and 2 are correct'.

Select the correct option:

Study the following statements regarding chemiosmotic hypothesis in mitochondria and select the correct ones.
(i) ${F} _{1}$ headpiece contains the site for the syntesis of ATP from ADP+Pi.
(ii) ${F} _{0}$ part forms the channel through which protons cross the inner membrane.
(iii) For each ATP produced, ${2H}^{+}$ pass through ${F} _{0}$ from the intermembrane space to the matrix down the electrochemical proton gradient.

  1. (i) and (ii)

  2. (ii) and (iii)

  3. (i) and (iii)

  4. (i),(ii) and (iii)


Correct Option: D
Explanation:

  In the given question, the correct statements are as follows:

(i) F1 headpiece contains the site for the synthesis of ATP from ADP+Pi.

(ii) F0 part forms the channel through which protons cross the inner membrane.

(iii) For each ATP produced, 2H+ pass through F0 from the intermembrane space to the matrix down the electrochemical proton gradient.

So, the correct answer is '(i),(ii) and (iii)'.

Which one respiratory complex is coupled with ETS for ATP synthesis?

  1. Complex I

  2. Complex III

  3. Complex IV

  4. Complex V


Correct Option: D
Explanation:

ATP synthase is also called complex V. It is the final enzyme in the oxidative phosphorylation pathway and utilizes the energy stored in a proton gradient across a membrane to drive the synthesis of ATP from ADP and phosphate (Pi).

So, the correct option is 'Complex V'.

Breakdown of proton gradient developed during chemiosmosis leads to a halt in the release of 
  1. Oxygen

  2. electrons

  3. Energy

  4. Protons


Correct Option: C
Explanation:

According to the chemiosmotic theory of Peter Mitchell, a proton gradient created by the ETS across the membrane leads to the passive movement of protons from the intermembrane space into the matrix or the stroma, through ATP synthase present on the inner membrane. The passive energy released by the protons is used for the synthesis of ATP from ADP. If a proton gradient breaksdown there will be no synthesis of ATP and no movement of protons across the membrane.

So, the correct answer is 'Energy'

Which of the following is an incorrect combination of organism with its chromosome number in mieocyte and in gamete?

  1. Onion, $24, 12$

  2. Ophioglossum, $1260, 630$

  3. Human beings, $46, 23$

  4. Fruit fly, $8, 4$


Correct Option: A
Explanation:

Chromosome number in meiocyte $(2n)$ of onion cell is $32$, while chromosome number in gamete $(n)$ is $16$.

Which part of the plant takes in carbon dioxide from the air for photosynthesis.

  1. Root hari

  2. Stomata

  3. Leaf veins

  4. Sepals


Correct Option: A

Light energy is not used directly or indirectly in which of the following option?

  1. Splitting of ${ H } _{ 2 }O$

  2. To develop proton gradient during photophosphorylation

  3. ${ CO } _{ 2 }$ reduction in dark reaction

  4. To develop proton gradient during oxidative photophosphorylation


Correct Option: D
Explanation:

NADH and FADH$ _{2}$ transfer their electrons to molecules near the beginning of the transport chain. As electrons are passed down the chain, they move from a higher to a lower energy level, releasing energy. Some of the energy is used to pump H$^{+}$ ions, moving them out of the matrix and into the intermembrane space. The energy released in these reactions is captured as a proton gradient, which is then used to make ATP in a process called chemiosmosis. Together, the electron transport chain and chemiosmosis make up oxidative phosphorylation. 

So, the correct option is 'To develop proton gradient during oxidative photophosphorylation'.

Which theory explains ATP synthesis in chloroplasts and mitochondria?

  1. Lipman and Lohmann theory.

  2. Lock and Key theory of Fischer.

  3. Induced fit theory of Koshland.

  4. Chemiosmotic theory of Mitchell.


Correct Option: D
Explanation:

The chemiosmotic theory explains how ATP is generated from mitochondria by electron transport chain. The chemiosmotic theory was developed by the British biochemist, Peter Mitchell, to explain the mechanism of oxidative phosphorylation in mitochondria (and photophosphorylation in chloroplasts). 

So the correct option is 'Chemiosmotic theory of Mitchell.'

State whether the following statements are true or false .
ATP is a protein that supplies energy to the cell.

  1. True

  2. False


Correct Option: B
Explanation:

ATP is not a protein, it is a nucleotide. Proteins are made up of amino acids. While ATP is a nucleotide consisting of an adenine base attached to a ribose sugar, which is attached to three phosphate groups. 

So, the correct option is 'False'.

Read the following statements and find out the incorrect statement.

  1. Glucose is prepared at the source by photosynthesis which is converted to sucrose

  2. Loading at the source produces a hypotonic condition in phloem

  3. Loading of the phleom sets up a water potential gradient that facilitates the mass movement in the water

  4. Phloem tissue is composed of sieve tube cells, which form long columns with holes in their end walls called sieve plates


Correct Option: A

Continued evolution of $O _2$ in suspension of an isolated chloroplast in light in the presence of ferric salt, violate dyes, etc. is called

  1. Oxygenation

  2. Blackmans reaction

  3. Hills reaction

  4. Emerson effect


Correct Option: C
Explanation:

The Hill reaction is defined as the photoreduction of an electron acceptor by the hydrogens of water, with the evolution of oxygen. In vivo, or in the organism the final electron acceptor is NADP+. We can measure the rate of the Hill reaction in isolated chloroplasts. first demonstrated by Robert Hill in 1939, in which illuminated chloroplasts evolve oxygen when incubated in the presence of an artificial electron acceptor (e.g. Ferricyanide). The reaction is a property of photosystem II. 

So, the correct option is, 'Hills reaction'.

State whether the following statements are true or false .
The energy currency of a cell is adenosine triphosphate (ATP). ATP is chemically a lipoprotein.

  1. True

  2. False


Correct Option: B
Explanation:
lipoprotein is a biomolecule that is a combination of fat and protein. While ATP is a nucleotide consisting of an adenine base attached to a ribose sugar, which is attached to three phosphate groups. 
So, the correct option is 'False'.

Which of the following cell organelles in concerned with photophosphorylation?

  1. Mitochondria

  2. Plastochondria

  3. Chloroplast

  4. More than one option is concerned


Correct Option: C
Explanation:

The synthesis of ATP is called phosphorylation. If it occurs in the presence of light, it is called photophosphorylation, that occurs during photosynthesis in the chloroplast. Mitochondria perform oxidative and substrate level phosphorylation.

Hence, the correct answer is 'Chloroplast'

Which option is not correct for chemiosmotic theory?

  1. ATP synthesis is linked with proton gradient

  2. Break down of proton gradient releases energy

  3. ATPase enzyme act as pump to break proton gradient

  4. It is applicable for photo phosphorylation and oxidative phosphorylation


Correct Option: C
Explanation:

  • There is a proton gradient created across the thylakoid membrane. The breakdown of this gradient is important because it leads to the release of energy. The gradient is broken down due to the movement of protons across the membrane to the stroma through the transmembrane channel ADP ATP of the F0 of the ATPase. 
  • The ATPase enzyme consists of two parts: F0 and F1. This breakdown provides enough energy to cause a conformational change in the F1 particle of the ATPase, which makes the enzyme synthesize ATP.

So, the correct option is 'ATPase enzyme act as a pump to break proton gradient'.

How many ATP - synthesised during complete oxidation of 3 molecule acetyl co A :

  1. 12 ATP

  2. 24 ATP

  3. 36 ATP

  4. 6 ATP


Correct Option: A

The total number of ATP produced when one molecule of glucose is partially oxidised into lactic acid is: 

  1. 2

  2. 10

  3. 8

  4. 36


Correct Option: A

Read the following statements : 
a) $F _0$ part of ATPase is associated with breakdown of proton gradient.
b) A H - carrier contributes in creation of proton gradient.
c) Movement of electrons in ETS is coupled to pumping of protons into the lumen.
d) Formation of $NADPH + H^+$ is related with the creation of proton gradient.
How many of the above statements are correct ?

  1. Two

  2. One

  3. Four

  4. Three


Correct Option: A

Centrioles, cilia, flagella, and basal bodies have remarkably similar structural elements and arrangements. This leads us to which of the following as a probable hypothesis ?

  1. Disruption of one of these types of structure should necessarily disrupt each of the others as well.

  2. Loss of basal bodies should lead to loss of all cilia, flagella and centrioles

  3. Motor proteins such as dynein must have evolved before any of these four kinds of structure.

  4. Natural selection for motility must select for microtubular arrays in circular patterns.


Correct Option: A,D

Which of the following is not required for chemiosmotic process?

  1. Proton gradient

  2. Spliting of water

  3. ATP synthase enzyme

  4. Closed membrane system


Correct Option: D
Explanation:
A. Proton gradient is required because the breakdown of this gradient leads to release of energy.
B. Splitting of water is required which takes place on the inner side of the membrane and the protons that are produced, they accumulate within the lumen of the thyllakoids.
C. ATP synthase enzyme is required that allows diffusion of protons back across the membrane, this releases energy to activate ATPase enzyme which catalyses the formation of ATP.
D. Closed membrane system is not required. Only a membrane is required for the development of a proton gradient across the membrane of the thyllakoid and the proton accumulation is towards the inside of the membrane.
So, the correct answer is 'Closed membrane system'.

How many ATP molecule could maximally be generated from one molecule of glucose, if the complete oxidation of one mole of glucose to $CO _{2}$ and $H _{2}O$ yields $686$ kcal and useful chemical energy available in the high energy phosphate bond of one mole of ATP is $12\ kcal$.

  1. Fifty-seven

  2. One

  3. Two

  4. Thirty


Correct Option: A
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