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Hgp and dna fingerprinting - class-XII

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Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ___ only ____ when the sequence __  is present in the DNA.
(ii) Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively _
, ____ and .
(iii) Since DNA has a _
charge, it moves towards the ____ of the electrophoretic chamber.

  1. (i) G A, GAATTC (ii) endonuclease, cellulase, chitinase (iii) negative, anode

  2. (i) G A, GAATTC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode

  3. (i) G A, GAATTC (ii) lysozyme, cellulase, chitinase negative/anode

  4. (i) G A, GAAATC (ii) lysozyme, cellulase, chitinase (iii) positive, cathode


Correct Option: C
Explanation:

  • EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA.
  • Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes lysozyme, cellulase and chitinases respectively.
  • Since DNA has a negative charge , it moves towards the anode of the electrophoretic chamber.
So, the correct option is 'Option C' .

Choose the correct answer from the alternatives given :
Match column I with column II and select the correct option from the given codes.

Column I(Scientists) Column II(Discoveries)
A. Alec Jeffreys (i) Lac operon
B. F. Sanger (ii) Annotated DNA sequences
C. Jacob and Monod (iii) DNA fingerprinting
D. Avery, MacLeod and McCarty (iv) Transforming principle
  1. A - (ii), B - (iii), C - (iv), D - (i)

  2. A - (iii), B - (ii), C - (i), D - (iv)

  3. A - (iii), B - (ii), C - (iv), D - (i)

  4. A - (i), B - (ii), C - (iii), D - (iv)


Correct Option: B
Explanation:

Alec Jeffreys  is a British geneticist, who developed techniques for genetic fingerprinting and DNA profiling which are now used worldwide in forensic science to assist police detective work and to resolve paternity and immigration disputes. F.Sanger developed the Sanger dideoxy method of sequencing DNA and also is credited for the sequencing of fragments of automated DNA sequences called annotated DNA sequences. This was very helpful for the human genome project that could sequence the entire genetic makeup of the human being.  In 1961, Francois Jacob and Jacques Monod proposed the operon model of gene regulation in bacteria. The model was based on their study of the genes in E. coli that code for enzymes that affect the breakdown of lactose also called the Lac Operon. Avery, MacLeod and McCarty experimentally proved that DNA is the substance or the genetic material that causes transformation in bacteria. 

So, the correct option is 'A - (iii), B - (ii), C - (i), D - (iv)'.

DNA fingerprinting can't be performed without 

  1. Restriction endonucleases

  2. Agarose

  3. Monoclonal antibodies

  4. Both (a) and (b)


Correct Option: A

Which of the following is not associated with DNA fingerprinting?

  1. Southern blotting

  2. Gel electrophoresis

  3. Restriction digestion

  4. DNA sequencing


Correct Option: A

A biotechnological process that has found a significant application in the field of forensic science is

  1. Recombination DNA technology

  2. Tissue culture

  3. DNA fingerprint technology

  4. Genetic engineering


Correct Option: C
Explanation:
  • DNA fingerprinting also called DNA typing, DNA profiling, genetic fingerprinting, genotyping, or identity testing, in genetics is a method of isolating and identifying variable elements within the base-pair sequence of DNA (deoxyribonucleic acid). 
  • DNA fingerprinting is currently used both for identifying paternity or maternity and for identifying criminals or victims.

VNTR is employed for

  1. Protoplasm culture

  2. DNA finger printing

  3. Regulation of plant growth hormones

  4. Enhancing photosynthesis in desert plant


Correct Option: B
Explanation:

A variable number tandem repeat (VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. For the purpose, restriction enzyme digested DNA samples are sorted by gel electrophoresis followed by southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition; option B is correct.  

Protoplasm culture is the fusion of protoplasm of two different species /individuals to bring their traits together in the hybrid. 
Plant growth hormones are the natural as well as artificially synthesized chemicals that control plant growth and development. Genes for abiotic stress tolerance factors such as glycine betaine are cloned in desert plants to increase their photosynthetic capacity by enhancing their tolerance for abiotic stress. The correct answer is B.

Matching DNA sequence of criminal with suspect is known as

  1. DNA finger printing

  2. DNA amplification

  3. Gene mapping

  4. DNA resolution


Correct Option: A
Explanation:

DNA amplification is the synthesis of multiple copies of small DNA sample which is done by PCR technique. It amplifies the gene of interest in three steps namely denaturation of target DNA (thermal cycle to separate the DNA strands), annealing of primers to the ssDNA and polymerization (extension of primer into complete DNA strand complementary to the template strand). 

Gene mapping is identifying the location of different genes on a chromosome. It is done using recombination frequency (which does not correspond to physical distance) or using RFLPs that generate physical maps. 
The DNA polymorphism in simple-sequence DNA and VNTRs has revealed during DNA finger printing in the identification of suspect since it is very remote possibility to have two individuals same repeats of satellite DNA throughout the genome except they share a biological relationship. This is because a child gets half DNA from father and half from the mother. Restriction enzymes of genome produce the restriction fragments length polymorphisms, or RFLPs depending on the location of restriction sites which are then sorted by gel electrophoresis followed by southern blotting. The blots of the suspect and of the sample from the crime scene are compared for sequence homology using radioactive probes; option A is the correct answer.

Which of the following can result in inaccurate test results in DNA fingerprinting?

  1. Sample size

  2. Tissue decay

  3. Blood transfussion

  4. All of the above


Correct Option: D
Explanation:

The possibility of having inaccurate test results increases if a very small sample of DNA is available for testing. The chance that the DNA will be mixed up with some other DNA is also higher with a smaller sample. If a person had a blood transfusion within the past 3 months which make the availability of DNA sample of different types. The decay of the tissue sample can also lead to inaccurate results. Therefore, the correct answer is option D.

What is meant by DNA fingerprinting ?

  1. Techniques used for molecular analysis of different specimens of RNA

  2. Techniques used for identification of fingerprints of individuals

  3. Molecular analysis of profiles of DNA samples

  4. Analysis of DNA samples using imprinting devices


Correct Option: C
Explanation:

DNA finger printing is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. This method was developed by Alec Jeffrey.

Which one of the following pairs of terms/names mean one and the same thing?

  1. Gene pool - Genome

  2. Codon - Gene

  3. Cistron - Triplet

  4. DNA fingerprinting - DNA profiling


Correct Option: D
Explanation:

DNA profiling or fingerprinting is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles.

Who developed DNA finger printing technique ?

  1. Jeffreys, Wilson and Thien

  2. Boysen and Jensen

  3. Schleiden and Schwann

  4. Edward and Steptoe


Correct Option: A
Explanation:

DNA fingerprinting technique was invented by Alec Jeffrey, Wilson and Thien.

Father of DNA finger printing is

  1. Sunder Lai Bahugana

  2. Wishwanath

  3. Jeffreys

  4. Rockfeller


Correct Option: C

Dermatoglyphics is connected with

  1. Skin diseases

  2. Care of skin

  3. Cosmetics

  4. Finger printing


Correct Option: D

VNTR is employed for

  1. Protoplasmic culture

  2. DNA finger printing

  3. Regulation of plant growth hormones

  4. Enhancing photosynthesis in desert plant


Correct Option: B

Best method to determine paternity is

  1. Protein analysis

  2. Chromosome counting

  3. Gene counting

  4. DNA finger printing


Correct Option: D

DNA finger printing is related to

  1. Molecular analysis of profiles of DNA samples

  2. Analysis of DNA samples using imprinting devices

  3. Techniques used for molecular analysis of different specimens of DNA

  4. Techniques used in identification of finger prints of different persons


Correct Option: A

DNA finger printing technique was developed by

  1. Jeffreys et al

  2. Schleiden and Schwann

  3. Boysen and Jensen

  4. Edwards and Steptoe


Correct Option: A

Matching DNA sequence of criminal with suspect is known as

  1. DNA finger printing

  2. DNA amplification

  3. Gene mapping

  4. DNA resolution


Correct Option: A

One of the following is major requirement for DNA finger printing

  1. Electron microscopy

  2. Electrophoresis

  3. ELISA

  4. HPLC


Correct Option: B

Pairing of DNA fragments is

  1. Staggering

  2. Annealing

  3. Augmenting

  4. Fragmenting


Correct Option: B

Which is not an application of DNA finger printing

  1. Solving immigration cases

  2. Solving parternity cases

  3. Curing SCID

  4. Identifying gene mutation


Correct Option: C

DNA finger printing requires

  1. Restriction endonuclease

  2. Taq polymerase

  3. Oligonucleotide primers

  4. All the above


Correct Option: D

Which is useful in solving parental disputes 

  1. Hybridoma technology

  2. DNA finger printing

  3. Western blotting

  4. ELISA


Correct Option: B

DNA finger printing in forensic science was pioneered in India by

  1. Lalji

  2. Khorana

  3. Bose

  4. Swaminathan


Correct Option: A

Some steps of DNA finger printing are given below. Identify the correct sequence from options given
a) Electrophoresis of DNA fragments
b) Hybridisation with DNA probe
c) Digestion of DNA by RENs
d) Autoradiography
e) Blotting of DNA fragments to nitrocellulose membrane

  1. c-a-e- b-d

  2. a-c- e - d-b

  3. c-a-b- e-d

  4. a-e-c-b-d


Correct Option: A

In DNA finger printing, the process of DNA hybridisation with the help of specific DNA probe is called

  1. Western blotting

  2. Northern blotting

  3. Southern blotting

  4. All the above


Correct Option: C

Which takes place in DNA finger printing

  1. Multiple restriction enzymes generate unique fragments

  2. Polymerase chain reaction amplifies fewer DNA

  3. Variability of repeated sequences between two restriction sites is evaluated

  4. A positive identification can be made


Correct Option: C

Select the correct statements for DNA finger printing
i) It is ELISA based technique
(ii) It is PCR based technique
(iii) It is used by forensic scientists
(iv) It is based on finger prints of the individual
(v) It is a test for paternity.

  1. i, ii, iii

  2. ii, iii, v

  3. i, iv, v

  4. i, iii, iv


Correct Option: B

Arrange in a sequence the given protocol for DNA finger printing
(a) Denaturating DNA (b) Blotting (c) Extraction of DNA (d) Fragmenting DNA (e) Separation of DNA fragments by electrophoresis (f) Hybridisation with probe (g) Exposure on film to make a DNA finger print

  1. c-d-e-a-b-f-g

  2. a-c-d-e- b -f-g

  3. a- c- b - d-e-f-g

  4. c-e-d-b-a-f-g


Correct Option: A

Assertion:  DNA fingerprinting involves identifying differences in some specific regions in DNA sequence.

Reason:  In repetitive DNA sequences, a small stretch of DNA is repeated many times.

  1. If both the assertion and the reason are true and the reason

    is a correct explanation of the assertion

  2. If both the assertion and reason are true but the reason is

    not a correct explanation of the assertion

  3. If the assertion is true but the reason is false

  4. If both the assertion and reason are false

  5. If the assertion is false but reason is true


Correct Option: B

'DNA fingerprinting' has proved to be useful in forensic science. It involves the use of _______________.

  1. Minisatellites

  2. Ribosomal RNA

  3. cDNA

  4. Bacterial DNA


Correct Option: A

Which one of the following techniques is used for identifying a DNA sequence that binds to a particular protein and is based on the idea that the phosphodiester bonds in the region covered by the protein are protected from digestion by deoxyribonucleic?

  1. Finger printing

  2. Foot printing

  3. RAPD

  4. FISH


Correct Option: A

The technique of DNA finger printing was initially developed by 

  1. lan wilmut

  2. Hargobind Khorana

  3. Jacque Monod

  4. Alec Jeffreys

  5. Francois Jacob


Correct Option: D
Explanation:

DNA profiling is the process of determining an individual's DNA characteristics, which are as unique as fingerprints. The technique was developed in 1984 by British geneticist Alec Jeffreys. 

So, the correct option is D, 'Alec Jeffreys'.

Basis of DNA fingerprinting is

  1. relative proportion of purines and pyrimidines

  2. relative difference in DNA occurrence in blood, skin and saliva

  3. relative amounts of DNA in ridges and groves of fingerprints

  4. satellite DNA occuring as highly short DNA segments.


Correct Option: D
Explanation:

By cutting a sample of DNA into fragments and comparing it with other samples it's possible to work out if the DNA has come from the same person. This technique is called DNA fingerprinting, and is a technique used to help solve crimes where the suspect may have left a sample of body tissue at the crime scene. Satellite DNA consists of very large arrays of tandemly repeating, non-coding DNA. Satellite DNA is the main component of functional centromeres, and form the main structural constituent of heterochromatin. The term satellite is used to describe DNA sequences that comprise short head-to-tail tandem repeats incorporating specific motifs. So, the correct option is "Satellite DNA occurring as highly short DNA segments."

Which of the following is incorrect about DNA finger printing?

  1. It is not inherited from parents to offspring

  2. Show high degree of polymorphism

  3. It is used to detect sex during fetal development

  4. It is used in medico-legal suits


Correct Option: C

Biometry refers to

  1. Identification of humans by scanning face and fingerprints

  2. Measurement of mechanical displacement in humans

  3. A method of lie detection

  4. Body length relationships across the evolutionary scale


Correct Option: A
Explanation:

Biometric recognition (also known as biometrics) refers to the automated recognition of individuals based on their biological and behavioral traits. Examples of biometric traits include fingerprint, face, iris, palmprint, retina, hand geometry, voice, signature and gait.

DNA fingerprinting is useful for the study of 

  1. Polymorphism

  2. Forensics

  3. Identity and relationship

  4. All of the above


Correct Option: D
Explanation:

DNA or genetic fingerprinting relies on the principle that no two individuals share the same genetic code except for identical twins. DNA fingerprinting uses the pattern of DNA fragments caused by specific enzymes to identify individual organisms: humans, other animals, plants or any other organism with DNA. DNA fingerprinting is useful in paternity analysis, criminal cases, and many studies of ecology and evolution.

Which is the most reliable method for identifying a person?

  1. Karyotype examination

  2. DNA fingerprinting

  3. Antibody fingerprinting

  4. All of the above


Correct Option: B
Explanation:

Fingerprinting is a very useful method in identification of a person. A fingerprint is unique to an individual and is permanent. The best method is DNA fingerprinting. One of the most common DNA fingerprinting procedures is RFLP (Restriction Fragment Length Polymorphism). Special enzymes are used to cut segments of a sample from which DNA is extracted. The procedure of RFLP focuses on repetitious sequences of DNA bases which vary greatly from individual to individual.

DNA fingerprinting technique was first developed by 

  1. Boysen and Jensen

  2. Jeffreys, Wilson and Thien

  3. Schleiden and Schwann

  4. Edward and Steptoe


Correct Option: B
Explanation:
British geneticist Sir Alec Jeffrey is widely considered the father of DNA fingerprinting – a technique that has revolutionized forensic science. 
Therefore, the correct answer is option B.

Which one of the following, scientists developed the process of DNA fingerprinting?

  1. Kary B. Mullis

  2. T. H. Morgan

  3. H. O. Smith

  4. Alec Jeffreys


Correct Option: D
Explanation:

In 1984, Alec Jeffreys discovered the technique of genetic fingerprinting in a laboratory in the Department of Genetics at the University of Leicester. Professor Sir Alec John Jeffreys, FRS is a British geneticist, who developed techniques for DNA fingerprinting and DNA profiling which are now used all over the world in forensic science to assist police detective work.

DNA profiling can be carried out using

  1. Blood

  2. Saliva

  3. Both A and B

  4. None of the above


Correct Option: C
Explanation:

DNA profiling can be carried out using a sample such as blood, saliva, semen or other appropriate fluid or tissue from personal items (e.g., a toothbrush, razor) or from stored samples (e.g., banked sperm or biopsy tissue). Samples obtained from blood relatives (related by birth, not marriage) can provide an indication of an individual's profile, as could human remains that had been previously profiled.

Which one of the following scientists developed the process of DNA fingerprinting?

  1. Kary B. Mullis

  2. T. H. Morgan

  3. H. O. Smith

  4. Alec Jeffreys


Correct Option: D
Explanation:

Sir Alec Jeffreys, professor of genetics is the pioneer of DNA profiling. DNA profiling (also called as DNA fingerprinting) is a forensic technique used to identify individuals by characteristics of their DNA.

The commonly used DNA fingerprinting technique in forensic science study is

  1. Southern blotting

  2. Northern blotting

  3. Eastern blotting

  4. Western blotting


Correct Option: A
Explanation:

Southern blotting and hybridization with probes can be used to locate a few specific fragments in a large pool of DNA. Southern blotting (named after Edwin M. Southern) is one technique used to transfer the denatured, single-stranded fragments from a gel to a thin solid medium.

DNA fingerprinting is useful for the study of

  1. Forensic science

  2. Identity

  3. Relationship

  4. All of the above


Correct Option: D
Explanation:

DNA fingerprinting is a method used in the identification of the genetic information (DNA) in a person's cell. It is a forensic technique used to identify an individual by the characteristics of the DNA. It is also useful to identify the identity and relation of an individual with others.

The technique used to increase the amount of DNA available for analysis is
I. Cloning a plasmid
II. Gel electrophoresis
III. PCR

  1. II only

  2. I and II

  3. I and III

  4. II and III

  5. I, II, and III


Correct Option: C
Explanation:
The polymerase chain reaction (PCR) is a technique used in molecular biology to amplify a single copy of DNA to millions of copies of a particular DNA sequence. 
Molecular cloning is a method used to assemble recombinant DNA molecules and to direct their replication within host organisms to increase the number of recombinant DNA molecule. Here, the DNA can be cloned in a plasmid (extracellular DNA).
Thus, the correct answer is option C.


Which of the following is not required for any of the techniques of DNA finger printing available at present

  1. DNA-DNA hybridisation

  2. Polymerase chain reaction

  3. Zinc finger analysis

  4. Restriction enzymes


Correct Option: C
Explanation:
A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn2+) in order to stabilize the fold. This proves that it does not require any DNA fingerprinting techniques.
So the correct option is 'Zinc finger analysis'.
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