Loss of $k^{+} ions$ decreases the osmotic concentration of guard cells as compared to adjacent epidermal cells. This causes exosmosis and hence turgidity of guard cells decreases.

In higher plants transpiration occurs through stomata. The stomatal pore is regulated by the turgidity of guard cells. When the guard cells are turgid the stomatal pores open. When guard cells are flaccid, stomatal pore closes.

Loss of $k^{+}$ ions decreases the osmotic concentration of guard cells as compared to adjacent epidermal cells. This causes exosmosis and hence turgidity of guard cell decreases.

Usually in angiosperms stomata open during daytime and are closed during night.

Proton transport concept in stomata was given by Levitt and is most widely accepted theory.
According to this theory, protons formed due to malic acid dissociation in guard cells are transported to subsidiary cells and in exchange potassium ions move into guard cells. This ion exchange requires ATP. The potassium ions are osmotically active. Their influx into the guard cells increases osmotic pressure of guard cells and results in endosmosis, which opens the stomata.

Most widely accepted theory for opening and closing of guard cells is the malate or potassium ion pump theory, first proposed by Levitt, 1974. According to this theory, during day time, rise in pH of guard cells (due to photosynthesis and consequent decrease of carbon dioxide) causes hydrolysis of starch to form phospho enol pyruvate (PEP). PEP combines with carbon dioxide to form organic acids like oxalic acid. The organic acids get ionised and the proton passes out of guard cells and potassium ion is actively absorbed and accumulated. Potassium ions combine with malate and passes into small vacuoles. Chloride ions are also absorbed from outside to maintain electroneutrality. As a result of accumulation of ions there is a reduction in water potential of guard cells. The reduction in water potential causes absorption of water from adjacent cells, guard cells become turgid and stomata open.

Scotoactive stomata are the stomata which open in dark and close during the day time. Scotoactive stomata occur in succulent like Cactus, Bryophyllum, Opuntia. The succulents perform crassulacean acid metabolism or CAM type of photosynthesis and their stomata are accordingly modified.

Scotoactive stomata are the stomata, which open in dark and close during the day time. 

Levitt in 1974, proposed a synthetic theory on the mechanism of stomatal movement, taking all earlier observations into account. The observed facts are:

(a) During opening of stoma, starch disappears from the guard cells.

(b) Photoactive opening is dependent on the chloroplast of the guard cells.

(c) Low $CO _2$ concentration causes stomatal opening.

(d) High pH in guard cells cause stomatal opening.

(e) There is a direct relation between $K^+$ accumulation and stomatal opening.

Opening and closing of stomata are controlled by the turgor changes in the guard cells. In the dark, the guard cells lose water, become flaccid and the stomata close. In the light, the guard cells absorb water by osmosis, become turgid and the stomata open.

Glucose increases osmotic concentration of guard cells. On account of it, the guard cells absorb water from neighbouring cells, swell up and create a pole in between them.

Malate or $k^{+}$ ion pump hypothesis theory was put forward by Levitt $(1974)$. It is considered as the modern one. It states that the change in the turgor pressure of the guard cells regulates the opening and closing of stomata. It appears to be an active mechanism which needs ATP.

• Guard cells help for regulating the rate of transpiration by opening and closing the stomata. 
• When the guard cells are swollen, then this turgidity is due to the accumulation of K ions in guard cells. 
• As K ion level increases, the water potential of guard cells also drops and then water enters into the guard cells. 
• Then it requires the energy which is utilized from ATP form of energy. Thus this needs a constant supply of ATP.
• So the correct answer is$ K^{+}$ and ATP.
• Correct option is B.

There are three more theories for mechanism of stomatal movement
(A) Hydrolysis of guard cell photosynthesis
(B) Classical starch hydrolysis theory
(C) Malate or $k^{+}$ ion pump hydrolysis.

Malate or $k^{+}$ ion pump hypothesis theory was put forward by levitt $(1974)$. It is considered as the modern one. It states that the change in the turgor pressure of the guard cells regulates the opening and closing of stomata. 

Glucose increases osmotic concentration of guard cells. On account of it, the guard cell absorb water from neighbouring cells, swell up and create a hole in between item.

• Potassium is a very important macronutrient for plants. Its ions cause the turgor movement of guard cells. 
• The gain of potassium ions by the guard cells causes a decrease in water potential of guard cells. 
• This makes guard cells turgid and hence they swell. Increase in turgidity of guard cells opens the stomatal pore. 
• Movement of potassium ions from the guard cells results in the increase of water potential. It becomes flaccid and stomatal pore closes. This mechanism of stomatal opening and closing was first stated by Levitt.

The correct option is B.

According to sugar concentration theory,during daytime ,the guard cells begin photosynthesis and the sugar produced during the process increases the osmotic pressure which draws in water from the adjoining cells due to endosmosis.

During daytime,the guard cells become turgid and buldge outwards due to their thin outer walls ,this widening the stomatal opening lying in between.

According to sugar concentration theory,during daytime ,the guard cells begin photosynthesis and the sugar produced during the process increases the osmotic pressure which draws in water from the adjoining cells due to endosmosis.

According to the sugar concentration theory, during the daytime, the guard cells do photosynthesis. And the sugar is produced during the process and it increases the osmotic pressure which draws in the water from the adjoining cells due to endosmosis.

So the correct option is Daytime.

The correct option is B.

Some stomata open during night and remain closed during the day time. This type of stomatal is called scotoactive stomata. They occur in succulents e.g., Cactus, Bryophyllum. This mechanism was explained by Nishida (1963).

Sayre reported that stomata open when pH is higher (7) and close when pH is low (5), i.i., high pH favours stomatal opening.

Porometer is an instrument for measuring the area of the stomatal openings of a leaf by the amount of a gas passing through a given area of it. The porometer was first described as a method for investigating stomatal movement by Darwin.

ABA is known to have a direct effect on the movement of stomata. When ABA is present, it does not allow the uptake of potassium ions which help in the opening of stomata and hence the pore closes.

Stomata opens by increasing solute concentration in guard cells. The guard cells contain chloroplasts, so they can manufacture food by photosynthesis. The epidermal cells don't have chloroplast. Due to the presence of chloroplast, guard cell synthesizes glucose during the day time by the process of photosynthesis and so its osmotic potential increases and it withdraws water from the epidermal cell and turns turgid and the stomata opens and transpiration takes place.

When the concentration of the potassium ions and malate ions increase guard cells form potassium malate and stores it in vacuoles of the guard cells. This increases the osmotic concentration and hence water enters the guard cells by endosmosis. The turgor pressure of the guard cells increases due to endosmosis which finally leads to the opening of the stomata.

The increased concentrations of potassium ions are balanced by malate ions that form potassium malate. This is stored in the vacuoles of the guard cells, increasing the osmotic concentration. Hence, the water enters the guard cells by endosmosis. The turgor pressure of the guard cells also increases due to this endosmosis causing the stomata to open.

When guard cells become turgid the inner wall cannot move in vertical direction or along the longitudinal axis. Neither it can move or stretch on inner side. This is because of extra cellulose deposition on inner wall and radial microfibrils emerging from the inner wall.
So as guard cells become turgid and pressure builds up, the outer wall is pushed outside, which pulls the inner wall towards outside, or radially outwards. This opens the stomatal pore.

Scotoactive stomata are the stomata, which open in dark and close during the day time. Scotoactive stomata occur in succulents, e.g., cactus and bryophyllum. Succulents perform crassulacean acid metabolism or CAM in their photosynthesis. Nishida explained in succulents during night there is accumulation of organic acids, whereas during day time the organic acids breakdown releasing carbon dioxide, which keeps stomata closed. During night, because of formation of organic acids carbon dioxide is consumed and concentration of carbon dioxide is less.

Scotoactive stomata are the stomata, which open in dark and close during the day time. Scotoactive stomata occur in succulents, e.g., cactus and bryophyllum. Succulents perform crassulacean acid metabolism or CAM in their photosynthesis. Nishida explained, that in succulents during night there is accumulation of organic acids, whereas, during day time the organic acids breakdown releasing carbon dioxide, which keeps stomata closed. During night, because of formation of organic acids carbon dioxide is consumed and concentration of carbon dioxide is less.

Stomata are tiny openings present on the epidermis of the leaves and young stem. These structures help in transpiration as well as uptake of carbon dioxide. The carbon dioxide is utilised in the process of photosynthesis. Since carbon dioxide is not utilised during the night, its concentration increases which inhibit the uptake of the potassium ions and closing of stomata takes place.