0

Use of microbes in biotechnology - class-X

Description: use of microbes in biotechnology
Number of Questions: 23
Created by:
Tags: botany biology principles and process of biotechnology biotechnology and genetic engineering useful and harmful microbes
Attempted 0/23 Correct 0 Score 0

The process of conversion of sugar into alcohol by yeast is called as

  1. Fermentation

  2. Pasteurisation

  3. Alcoholism

  4. All of the above


Correct Option: A
Explanation:

Fermentation is a metabolic process that converts sugar to acids, gases or alcohol. It occurs in yeast and bacteria, and also in oxygen-starved muscle cells, as in the case of lactic acid fermentation.

Pasteurisation is a

  1. Sterilisation technique

  2. Immunisation technique

  3. Fermentation technique

  4. None of the above


Correct Option: A
Explanation:

Pasteurization is the technique of heating milk at a high temperature ( 75$^0$ C) for a short period of time (15 s) and again cooling down quickly. It kills bacteria that may be in the milk, to ensure its safe consumption. Also, it increases the lifespan of milk. Therefore, pasteurisation is a 'sterilisation technique'.

Which of the following is used to disinfect contaminated utensils?

  1. 5% of phenol

  2. 30% of chloroform

  3. 1% isopropanol

  4. 70% of butanol


Correct Option: A
Explanation:

Phenol usually in low concentrations is used in household used for cleaning and disinfecting purpose.Chloroform can't be used for disinfecting utensils as it is an anesthetic drug and causes dizziness.Isopropanol is a flammable reagent and also can't be used as a cleaning product.Butanol is usually used in painting or other work as thinner and also its fumes are harmful if inhaled and may cause slight irritation. so this also can't be used for disinfecting utensils. So, the correct answer is'5% of phenol'

Which of these organisms is unicellular, causes fermentation of sugar solutions and respires anerobically?

  1. Rhizopus

  2. Yeast

  3. Amoeba

  4. Bacteria


Correct Option: B
Explanation:

The yeast is a type of fungal species which have unicellular cells. These cells are known to perform anaerobic fermentation of the sugars and produce alcohol and carbon dioxide. The organism respires anaerobically and breaks down the fermentable sugars in the absence of oxygen.

Thus, the correct answer is option B. 

Gamma rays are used to sterilize

  1. Pharmaceutical products

  2. Culture media

  3. Food

  4. Both A and C


Correct Option: D
Explanation:

Gamma rays are used for sterilization of pharmaceutical products after their production as to prevention the growth of any microorganism that may be present in the product. It is also used for sterlization of food product to kill any harmful bacteria or other organism that are present in the food, it won't make the food radioactive. Gamma rays act as a ionising radiation and it will inhibit the growth of microorganism. So it can be used to sterilize culture media. So, the correct answer is 'Both A and C'

Hops used in flavouring beer are.

  1. Fruits of Humulus

  2. Seeds of Humulus

  3. Dried petals of Humulus

  4. Dried sepals of Humulus


Correct Option: C
Explanation:

Hops used to provide a distinct flavour to beer are actually the flowers of Humulus lupulus

So the correct answer is  'Flowers of Humulus'

Aseptic culture means

  1. Absence of life

  2. Presence of bacteria

  3. Absence of other organisms like microbes

  4. Parthenogenetic development


Correct Option: C
Explanation:

Aseptic culture means culture which takes place in the absence of harmful microbes or other contaminants. During tissue culture, the cells are kept under the aseptic condition to avoid contamination caused by microbes because the microbes inhibit the growth of the cell and utilize the nutrients provided for the plants in the media for their growth as they have the ability to multiply fast. The aseptic condition during culture can be maintained by the process of heating, fumigation of lab, autoclave apparatus before use, wiping the apparatus with alcohol, performing the experiment in the laminar air flow under HEPA filters. 

So, the correct answer is option C.

Yeast was used in fermentation first of all by.

  1. Hansen

  2. Pasteur

  3. Spike

  4. Jackson


Correct Option: B

In tissue/bacterial culture glassware and nutrients are sterilised through

  1. Water bath at $200^o C$

  2. Dry air oven at $200^o C$

  3. Dehumidifier

  4. Autoclave.


Correct Option: D
Explanation:
Tissue/bacterial culture, glassware are all sterilized through autoclave. Autoclave steam sterilizes all the components under controlled pressure.
So, the correct answer is 'Autoclave'

Fermentation ability of yeast is due to

  1. Amylase

  2. Galactase

  3. Zymase

  4. Invertase


Correct Option: C
Explanation:

Zymase is the enzyme complex that catalyse the fermentation of sugar into ethanol and $CO _2$. It occur naturally in the yeast. Zymase activity varies as among the yeast strains.

It is important to boil surgical instruments before using them in an operation. It is done

  1. To kill all the pathogens which may be infecting the instruments.

  2. To facilitate the handling of the instruments by the doctor.

  3. To enable the patient feel warm and comfortable at the time of operation.

  4. To kill all the pathogens present at the place of operation of the patient.


Correct Option: A
Explanation:

It is important to boil surgical instruments before using them in an operation. It is done to kill all the pathogens which may be infecting the instruments. After the operation and surgery, the germs and pathogens are present on the instruments used during operation and surgery. So in order to kill those pathogens and bacterial cyst, the surgical instruments are boiled for 10 to 15 minutes. This will reduce the risk of transmission of disease from one person to another person.

Thus, the correct answer is 'To kill all the pathogens which may be infecting the instruments.'

Yeast is used in preparation of

  1. Acetic acid

  2. Curd

  3. Ethyl alcohol

  4. Cheese


Correct Option: C
Explanation:
Yeast is a fungal species that converts carbohydrates to carbon dioxide and alcohols.
(A)Acetic acid bacteria are responsible for the production of Acetic acid.
(B)Lactobacillus is responsible for the conversion of milk into curd.
(C)Saccharomyces cerevisiae is responsible for the production of ethyl alcohol.
(D)Lactobacillus or Streptococcus is responsible for the conversion of production of cheese.

So, the correct answer is 'Ethyl alcohol'

Product of fermentation of glucose by yeast is?

  1. $(C _6H _{10}O _5) _n$

  2. $C _2H _5OH$

  3. $C _6H _{12}O _6$

  4. $CH _3OH$


Correct Option: B
Explanation:

Yeast is a fungal species that converts carbohydrates to carbon dioxide and alcohols. Saccharomyces cerevisiae is responsible for the production of ethyl alcohol.

So, the correct answer is 'C2H5OH'C2H5OH

Which is not one of the new uses of biotechnology ?

  1. Making beer and wine

  2. Cleaning up oil spills

  3. Increasing soil fertility

  4. Obtaining minerals from mining


Correct Option: A
Explanation:

Making beer, wine, cheese, curd etc using microorganisms are classic methods of biotechnology I.e. using microbes for isolation. Of product of our use. While with advancement in technology, we have extended biotechnology to other purposes like Cleaning up oil spills, increasing soil fertility, obtaining minerals from mining etc. 

So, the correct option is 'Making beer and wine'.

A bacterial culture is treated with streptomycin and a cast is made. A few colonies are able to survive due to

  1. Selection

  2. Adaption

  3. Mutation

  4. Mimicry


Correct Option: B
Explanation:

Adaptation is the alteration in the structure or function of an organism or any of its parts that results from natural selection and by which the organism becomes better fitted to survive and multiply in its environment. The bacteria got adapted towards the changes and hence survived.

So the correct option is 'Adaption'.

Most commonly used substrate for industrial production of beer is __________.

  1. Barley

  2. Wheat

  3. Corn

  4. Sugarcane molasses


Correct Option: A

Tissue culture medium is sterilised by

  1. Antifungal agents

  2. Keeping at -20$^o$C

  3. Autoclaving at 120$^o$C for 15 minutes

  4. Filtering through fine sieve


Correct Option: C
Explanation:

Tissue culture is the growth of cells or tissues separated from an organism. This is facilitated using a liquid, semi-solid medium which is sterilized so as to prevent the contamination by autoclaving them. It is a process whereby applying heat and pressure, microbes are killed in a container.

So, the correct answer is 'Autoclaving at 120-degree Celsius for 15 minutes'

Which of the following yeasts is used in the preparation of beer and wine?

  1. Saccharomyces ludwigii

  2. Saccharomyces cerevisiae

  3. Schizosaccharomyces

  4. Ashbya gossypii


Correct Option: B
Explanation:

Saccharomyces cerevisiae (common yeast) is a species of yeast. It is the most useful yeast, linked to wine making, baking and brewing. It is also called as baker's yeast or brewer's yeast.

In a microbiology laboratory, the technician uses heat to sterilise the nutrient solution that is used to grow 4. a fungus. When the heating system broke down, he sterilised the solution by passing It on a sterile environment) through a sterile filter with a pore size of 0.2 micrometers. When the fungus was grown on the filtered nutrient solution, it stopped growing and looked unhealthy within a few days. Which statements is the most likely explanation for the observed effects on the fungus ?

  1. The nutrient solution contained a virus

  2. Heating makes the glucose in the nutrient solution more digestible

  3. Filtering removed one of the larger nutrients

  4. The nutrient solution contained a bacterium that was pathogenic to the fungus.


Correct Option: A
Explanation:

A) Viruses inhibit the growth of other organisms by entering the host organism and using its genetic material and nutrition to reproduce. Hence, the fungus stops growing.

B) The fungus absorbs glucose. Heating glucose will destroy it and the fungus will not be able to digest it.
C)  Glucose is a micro-molecule which passes through the filter. The fungus mainly requires glucose to survive. Filtering did not affect the fungus.
D) Bacteria are too large to penetrate the filter and hence cannot affect the fungus.
So the correct answer is 'The nutrient solution contained a virus'.

Which of the following is correct about mushroom?

  1. Moss

  2. Fern

  3. Yeast

  4. Pine

  5. Seaweed

  6. Fungi


Correct Option: F
Explanation:

Fungi are a separate class of microorganisms that reproduce by spores and are thus different from plants that require photosynthesis to make food for themselves. Fungi consist of molds, yeast, mildew, and mushrooms. It can be unicellular or multicellular. So all mushrooms are fungi, though not all fungi are mushrooms as there are much more types of fungi than mushrooms. Mushroom is not yeast.

The new strain of bacteria produced by biotechnology in alcohol industry is.

  1. Escheriachia coil

  2. Sacharomyces cerevisiae

  3. Bacillus subtills

  4. Pseudomonas putida


Correct Option: D

Progenitors formed in bone marrow but differentiating elsewhere are

  1. Pre-NK cells

  2. Pre-erythroblasts

  3. Pre-T cells

  4. Myeloblasts


Correct Option: C
Explanation:

Pre T cells are progenitors of T- lymphocytes which are produced from the bone marrow. Pre- T cells undergo differentiation and maturation in the thymus to form matured T- cells. T-cells can be distinguished from other lymphocytes by the presence of a T-cell receptor on the cell surface.  Hence, Progenitors formed in bone marrow but differentiating elsewhere are Pre-T cells.

So, the correct answer is 'Pre-T cells'.

Yeast is not an important source of

  1. vitamin C

  2. vitamin B

  3. vitamin A

  4. vitamin D


Correct Option: A,B,D
- Hide questions