DNA transfer - class-XII
Description: dna transfer | |
Number of Questions: 21 | |
Created by: Manjit Singh | |
Tags: principles and process of biotechnology biotechnology: principles and processes biotechnology and its application botany genetic engineering cell biology and biotechnology biology genetic technology |
Which of the following is required for microinjection method of gene transfer?
The term "competent" refers to
Micro-injection is a method used to
If a person obtains transformants by inserting a recombinant DNA within the coding sequence of enzyme -galactosidase, he will separate out recombinants from non-recombinants by which of the following observations?
In the process of insertional inactivation ________________.
If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the untransformed recipient cells will die. The product produced by transformant bacteria that digests the ampicillin antibiotic is called as -
The correct sequence of making a cell competent is
Statement 1 : In insertional inactivation, blue colour produced by bacterial colonies indicates that the plasmid does not have an insert into the bacterial genome.
Statement 2: Presence of insert results into insertional inactivation of -galactosidase enzyme and the colonies do not produce any colour.
rDNA is
Direct visual selection method for the selection of recombinant host cells on the basis of their inability to produce colour in the presence of chromogenic substrate can be applied if the vector used is a
Chimeric DNA is
Two bacteria found to be very useful in genetic engineering experiment are
Which of the following most used in genetic engineering?
Two bacteria most useful in genetic engineering are
Competent host in recombinant DNA technology is __________________.
Which of the following is mostly used in genetic engineering experiments?
The uptake of naked DNA by bacteria is called
R-II strain of $T 4$ bacteriophage cannot lyse Escherichia coli. Two of its types, R-$II^x$ and R-$II^y$ were allowed to invade the bacterium which lysed because of _________________.
The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of
Changes in transcription after linking the cloned DNA of the putative promoter/enhancer region to cloned DNA of the coding region of an unrelated gene, can be measured rapidly with a high degree of sensitivity using.
Which one of the following techniques made it possible to genetically engineer living organisms?