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Miscellaneous applications of biotechnology - class-XII

Description: miscellaneous applications of biotechnology
Number of Questions: 18
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Tags: biotechnology and its applications option b: biotechnology and bioinformatics biotechnology and its application biotechnology: general introduction biology applied biology zoology
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Which of the following can be classified as recognition transduction component of a biosensor?

  1. Amplifiers

  2. Bioreceptors

  3. Transducer

  4. None of the above


Correct Option: C
Explanation:
A biosensor is an analytical device, used for the detection of an analyte, that combines a biological component with a physicochemical detector. A biosensor typically consists of a biorecognition component, biotransducer component, and electronic system which include a signal amplifier, processor, and display. The transducer transforms the signal resulting from the interaction of the analyte with the biological element into another signal that can be more easily measured and quantified.
So, the correct answer is option C.

Tissues are used as a biosensor because

  1. Available at low prices

  2. Easier to immobilise

  3. Cofactors for the enzyme to function are already present

  4. All of the above


Correct Option: D
Explanation:
Tissues are used as a biosensor because of following advantages:
  • They are easier to immobilize.
  • They maintain high stability hence not degraded by various enzymes.
  • They are available at low prices.
  • Process of extraction, centrifuge and purification of enzymes can be avoided.
  • They are necessary co-factors.
So, the correct answer is option D.

Which of the following are squares of glass, smallest than the smallest postage stamp

  1. Biochip

  2. Proteomics

  3. Forensic technologies

  4. None


Correct Option: A
Explanation:

Biochips are squares of glass, smaller than smallest postage stamp, covered with millions of DNA strand. Biochip is a tool allowing rapid screening of gene profile to detect genes. They are called gene microarrays. A biochip contains all known human genes.

Is a tool allowing rapid screening of gene profile to detect genes.

  1. Biochip

  2. Proteomics

  3. Forensic technologies

  4. None


Correct Option: A
Explanation:

Biochips are squares of glass, smaller than smallest postage stamp, covered with millions of DNA strand.

Arrange the following parts of a biosensor in chronological order.

  1. A) Bioreceptors, Transducer, Amplifier, Microelectronics

  2. B) Bioreceptors, Amplifier, Microelectronics, Transducer

  3. C) Transducer, Bioreceptors, Amplifier, Microelectronics

  4. D) Bioreceptors, Amplifier, Microelectronics, Transducer


Correct Option: A
Explanation:
A biosensor typically consists of a biorecognition component, biotransducer component, and electronic system which include a signal amplifier, processor, and display. The bioreceptor is designed to interact with the specific analyte of interest to produce an effect measurable by the transducer. High selectivity for the analyte among a matrix of other chemical or biological components is a key requirement of the bioreceptor and it uses biomolecules from organisms or receptors modelled after biological systems to interact with the analyte of interest. This interaction is measured by the biotransducer which outputs a measurable signal proportional to the presence of the target analyte in the sample.
So, the correct answer is option A.

The type of bio transducer used in the biosensors are ___________.

  1. Electrochemical biosensors

  2. Piezoelectric biosensors

  3. Pyroelectric biosensors

  4. All of the above


Correct Option: D
Explanation:

The type of transducer used in the sensors are 


D) All of the above

i.e electrochemical, piezoelectric, pyroelectric, optical, amperometric biosensors.

The amperometric transducers used in biosensors is used to detect _________________.

  1. Change in current during electrochemical oxidation or reduction

  2. Change in conductive properties of the sample solution

  3. Change in potential proportional to the concentration of analyte

  4. None of the above


Correct Option: A
Explanation:

Amperometric transducers detect change in current as a result of electrochemical oxidation or reduction. 

Typically, the bioreceptor molecule is immobilized on the working electrode commonly gold, carbon, or platinum. 
The current produced is a direct measure of the rate of electron transfer.

The commercial blood glucose biosensor uses which of the following enzymes?

  1. Glucokinase

  2. Glucose oxidase

  3. Phosphofructokinase

  4. Glycosidases


Correct Option: B
Explanation:

Glucose oxidase is widely used for the determination of free glucose in body fluids and in the food industry. It also has many applications and typically enzyme assays for biochemistry including biosensors. 

The biological content which acts as a sensor in biosensor can be __________.

  1. Nucleic acid

  2. Plant proteins

  3. Lectins

  4. All of the above


Correct Option: D
Explanation:
  • The biological component of biosensor can be divided into two distinct groups, i.e., catalytic and non-catalytic.
  •  The catalytic group includes enzymes, microorganisms and tissues, while the non-catalytic consists of antibodies, receptors, nucleic acids and some proteins such as lectins etc. 
  • The recognition process is based on the principle of complementary base  pairing adenine:thymine and cytosine:guanine in DNA. 
  • If the target nucleic acid sequence is known,complementary sequences can be synthesized, labelled, and then immobilized on the sensor.
  • The most important applications of lectin-based biosensors are in the field of medicine and therapeutics. 
So, the correct answer is option D.

Why are enzymes considered as a good bioreceptors for biosensors?

  1. They have specific binding capabilities

  2. They have good catalytic power

  3. They can detect analytes

  4. All of the above


Correct Option: D
Explanation:

Bioreceptor interacts with the specific analyte of interest to produce an effect which is measured by the transducer. The main reasons for the common use of enzymes in biosensors are:

  • Ability to catalyze a large number of reactions;
  • Potential to detect a group of analytes such as substrates, products, inhibitors, and modulators of the catalytic activity and
  • Suitability with several different transduction methods for detecting the analyte
So, the correct answer is option D.

The common type of bioreceptor interaction involves _____________.

  1. Antigen-antibody

  2. Enzyme-ligand

  3. Nucleic acid- DNA

  4. All of the above


Correct Option: D

Oxidation of ethanol can be sensed by which of the following biosensor?

  1. Electrochemical biosensor

  2. Microbial biosensor

  3. Ozone biosensor

  4. None of the above


Correct Option: A
Explanation:

A- Electrochemical biosensors are simple tools which are utilized to determine the measurement of electronic current or conductance changes done by the bio-electrodes. The electrochemical sensor converts the electrochemical information into an analytical signal. This type of biosensor is generally made up of two basic parts - a biological component & an electronic component.

Blood glucose biosensor is an example of ___________.

  1. Conduct metric biosensor

  2. Potentiometric biosensor

  3. Amperometric biosensor

  4. None of the above


Correct Option: C
Explanation:
Amperometric biosensors are biosensors which function by the production of a current when a potential is applied between two electrodes. One of its application is the determination of glucose concentrations by the use of an immobilised glucose oxidase membrane. Reaction results in a reduction of the oxygen concentration as it diffuses through the biocatalytic membrane to the cathode, this being detected by a reduction in the current between the electrodes
So, the correct answer is option C.

Which of the following biosensors is used for medical diagnostics and forensic science?

  1. Ozone biosensor

  2. DNA biosensor

  3. Microbial biosensor

  4. Both B and C


Correct Option: B
Explanation:

DNA biosensors have various applications, commonly used for medical diagnostics, forensic science, agriculture, or even environmental clean-up efforts. It function as the two strands of DNA stick to each other by virtue of chemical attractive forces and that is, two strands that match up at every nucleotide position gives rise to a fluorescent signal that is then transmitted to a signal generator.

So, the correct answer is option B.

Consider the following statement.
Biosensors are used in:
a. In vitro diagnostics
b. Detection of microbial contaminants in food
c. Monitoring pollutants in the biotic component of environment.
Which of the statements given is/are correct?

  1. $1$ and $2$ only

  2. $2$ only

  3. $1$ and $3$ only

  4. $1, 2$ and $3$


Correct Option: A

Which of the enzyme can be used in electrochemical biosensors?

  1. Ligase

  2. Endonuclease

  3. Dehydrogenase

  4. None of the above


Correct Option: C
Explanation:

Electrochemical biosensors are those biosensors that are mostly based on enzymes and catalyses the reaction that produces or consumes electrons. The reactions are redox types and the enzymes that catalyze these oxidations are called dehydrogenases.

So, the correct answer is option C.

Which of the following is considered as a physical component of biosensor?

  1. Analyte

  2. Amplifier

  3. Transducer

  4. Both B and C


Correct Option: D

____ are miniaturized labs which help in detection of diseases.

  1. Biotech labs

  2. Biomolecular labs

  3. Biolabs

  4. Biochips


Correct Option: D
Explanation:

In molecular biology, biochips are essentially miniaturized laboratories that can perform hundreds or thousands of simultaneous biochemical reactions. Biochips enable researchers to quickly screen large numbers of biological analytes for a variety of purposes, from disease diagnosis to detection of bioterrorism agents.

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