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Tests for nutrients - class-VIII

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X is one of the important components of food. Wheat, pearl millet and sweet potato are rich sources of X, X gives a blue-black colour with a dilute solution of iodine. Identify X.

  1. Minerals

  2. Fats

  3. Proteins

  4. Carbohydrates


Correct Option: D
Explanation:

  • Starch gives blue-black colour with Iodine and is used as a test. Starch is a polysaccharide(carbohydrate).

So, the correct option is 'Carbohydrates'.

A green leaf is partially covered and placed in the light for several days. If it is then boiled and tested for starch with iodine, what will be the result?

  1. Starch will be found only in the shaded part of the leaf

  2. Starch will be found in the entire leaf

  3. Starch will be found only in the part of the leaf exposed to light

  4. Starch not be found in the leaf at all


Correct Option: C
Explanation:

The leaf on the right shows a positive test for starch in the areas which contained chlorophyll, and a negative test for starch in the areas which lack chlorophyll. This is evidence that chlorophyll is required for photosynthesis. A plant can be 'de-starched' by leaving it in the dark for a few hours. So, the correct option is "C" (Starch will be found only in the part of the leaf exposed to light).

Iodine gives blue-black colour with

  1. Protein

  2. Oil

  3. Starch

  4. Sucrose


Correct Option: C
Explanation:

The iodine test is used for the determination of the presence of starch in a sample. Molecular iodine is not soluble in water and hence potassium iodide is added to it. Together they form polyiodide complex which upon addition of starch turns intense blue-black colour. Starch is a polymer of amylose and amylopectin, of which amylose is responsible for the blue colour of the reaction. In the absence of starch solution remain as light brown colour which is the colour of iodine solution.

The digestive juices break down into simpler substance

  1. Proteins

  2. Hydrochloric acid

  3. Fatty acid

  4. None of the above


Correct Option: A
Explanation:

Gastric acid is a digestive fluid, formed in the stomach. It is composed of hydrochloric acid (HCl) 0.05–0.1 M (roughly 5,000–10,000 parts per million) potassium chloride (KCl) and sodium chloride (NaCl). The acid plays a key role in digestion of proteins, by activating digestive enzymes, and making ingested proteins unravel so that digestive enzymes break down the long chains of amino acids. The highly acidic environment in the stomach lumen causes proteins from food to lose their characteristic folded structure (or denature). This exposes the protein's peptide bonds. The gastric chief cells of the stomach secrete enzymes for protein breakdown (inactive pepsinogen, and in infancy rennin). Hydrochloric acid activates pepsinogen into the enzyme pepsin, which then helps digestion by breaking the bonds linking amino acids, a process known as proteolysis. In addition, many microorganisms have their growth inhibited by such an acidic environment, which is helpful to prevent infection. HCl is digestive juice. Fatty acids are digested by bile acids.

Therefore, the correct answer is option A.

Which carbohydrate gives red colour when iodine solution is added to it?

  1. Starch

  2. Glycogen

  3. Dextrin

  4. Insulin


Correct Option: B
Explanation:

Iodine test is used to distinguish between the presence of starch and glycogen and other saccharides.
When starch solution is treated with iodine, it gives a blue color. The blue color develops because of the trapping of iodine in the helical coils of amylose. When this test solution is heated, the color disappears due to disruption of the helical coils of amylose and the color reappears upon cooling, due to the reformation of helical coils. Glycogen however gives red color with iodine.
Therefore, the correct answer is option B.

Which of the following foods turns blue-black with iodine solution?

  1. Milk

  2. Groundnut

  3. Tomato

  4. Raw potato


Correct Option: D
Explanation:

  • Raw potato contains approximately 17% starch. when iodine solution added to the raw potato sample, it turns blue-black. Iodine reacts with the starch present in the raw potato which results in the formation of blue-black colored complex. 

Hence option D is the correct answer.

Egg white, turns violet when two drops of copper sulphate and ten drops of caustic soda is added to it. This indicates the presence of 

  1. Starch.

  2. Proteins.

  3. Fats.

  4. Vitamins.


Correct Option: B
Explanation:

When two drops of copper sulphate and ten drops of caustic soda or NaOH is added to egg, it turns violet. This indicates the presence of proteins.
This is Biuret's test and the principle is simple as follows:
In the presence of alkaline medium (caustic soda or NaOH), the Cu2+ ions get reduced to Cu+ and binds to the egg proteins, thereby giving a violet color.
Therefore, the correct answer is option B.

Which of the following agent is used to detect the presence of fat in the food product?

  1. Methyl alcohol

  2. Ethyl alcohol

  3. Sodium hydroxide

  4. Iodine solution


Correct Option: B
Explanation:

Shake or crush the food to make it dissolve. Filter or dilute the food and ethanol mix so that you get a clear liquid (a solution of fat in ethanol). Add this to a test tube of water. A white (milk-like) emulsion indicates the presence of fats or oils.

So, the correct option is 'Ethyl alcohol'.

Starch is tested with ................  solution.

  1. Caustic soda

  2. Salt

  3. HCl

  4. Iodine


Correct Option: D
Explanation:

The iodine test is used to test for the presence of starch. Iodine dissolved in an aqueous solution of potassium iodide reacts with the starch, producing a purple-black colour. The intensity of the colour decreases with increasing temperature and with the presence of water-soluble organic solvents, such as ethanol.

What compounds form fats and oils?

  1. Carboxylic acids +Alcohol

  2. Carbon Compounds +water

  3. Carbon Dioxide + Alcohol

  4. Carbon Monoxide +Alcohol


Correct Option: A

Which of the following is used to test presence of starch?

  1. Cheek peel

  2. Onion

  3. Potato

  4. None of the above


Correct Option: C
Explanation:

A chemical test for starch is to add iodine solution (red) and see if it turns blue/black in colour. It is possible to distinguish starch from other carbohydrates using this iodine solution test. For example, if iodine is added to an peeled potato then it will turn black. 

Which of the following test are used for detection of protein?

  1. Biuret test

  2. Iodine test

  3. Protein test

  4. Glucose test


Correct Option: A
Explanation:

The Biuret test is a chemical test used for detecting the presence of peptide bonds. In the presence of peptides, a copper (II) ion forms violet-coloured coordination complexes in an alkaline solution. The Biuret reaction can be used to assess the concentration of proteins because peptide bonds occur with the same frequency per amino acid in the peptide. 

Choose the correct answer from the alternatives given.
If an animal needed to store energy for long-term use, but not be encumbered with the weight of extra.tissue, which is the best molecule for storage?

  1. Fructose and glucose in the form of honey

  2. High-calorie fat molecules

  3. Complex cellulose molecules

  4. Glycogen with extensive side branches of glucose


Correct Option: B
Explanation:

If an animal needed to store energy for long-term use, but not be encumbered with the weight of extra tissue, then high-calorie fat molecules are the best molecules for storage. So, the correct answer is "high-calorie fat molecules".

Oil is obtained from the plant and animal sources can be detected by 

  1. Oil paper test

  2. Starch

  3. Calcium sulphate solution

  4. Iodine solution


Correct Option: A
Explanation:
Some plants and animal produce oil. Oil paper test is a test used to detect oil in plant and animal products. It include a process in which a paper is moved back and forth few times against the substance and the presence of oil on the paper is checked.
So the correct answer is 'Oil paper test'.

Which of the following helps in nutrient conservation?

  1. Leaching

  2. Mineralisation

  3. Nitrification

  4. Immobilisation


Correct Option: B,C
Explanation:

A.Leaching - the loss of water-soluble plant nutrients from the soil. It does not help in nutrient conservation.

B.Mineralisation – The process of formation of minerals from the organic compounds is called mineralisation. It helps in nutrient conservation.

C.Nitrification – Formation of nitrites or nitrates from ammonia or nitrogenous compounds is called Nitrification. It also helps in nutrient conservation.

D. Immobilisation – elements, cannot be transported from one place to another place in the body is called Immobilisation and the elements are called Immobilised elements.

So, the correct option is ‘Mineralisation or Nitrification’. 

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