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GATE Biotechnology

Description: Biotechnology GATE Biology Biochemistry Microbiology CSIR NET Life Sciences MicrobiologyRecombinant DNA TechnologyBiochemistryBioinformatics
Number of Questions: 20
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Tags: Biotechnology GATE Biology Biochemistry Microbiology CSIR NET Life Sciences Recombinant DNA Technology Bioinformatics
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Which of the following statements are false?

  1. Heat inactivation of serum is done to inactivate prions.
  2. Nude mice refer to transgenic mice.
  3. A humanised antibody is one in which the CDRs are from mouse, and the rest is from human.
  4. B-lymphocytes originate from the bone marrow.
  1. Only 1 and 2

  2. Only 1 and 3

  3. Only 2 and 3

  4. Only 2 and 4

  5. Only 3 and 4


Correct Option: A
Explanation:

Yes, this is the correct answer. Both are false statements. Heat-inactivation of serum is done by heating to 56°C for 30 minutes to inactivate complement (a group of proteins present in sera that are part of the immune response). A nude mouse is a laboratory mouse from a strain with a genetic mutation causing deteriorated or absent thymus. This type of genetic mutation results in an inhibited immune system due to a greatly reduced number of T cells.

Which of the following is a transcription related database?

  1. GDB

  2. HOMSTRAD

  3. JASPAR

  4. NDB

  5. OMIM


Correct Option: C
Explanation:

Correct answer. JASPAR is a collection of transcription factor DNA-binding preferences, modelled as matrices.

Which of the following are computational tools for eukaryotic genomic sequences?

  1. AUGUSTUS
  2. Chemgenome
  3. GeneMark
  4. mGene
  1. Only 1 and 2

  2. Only 1 and 4

  3. Only 2 and 3

  4. Only 2 and 4

  5. Only 3 and 4


Correct Option: B
Explanation:

This is the correct answer. AUGUSTUS is a program that predicts genes in eukaryotic genomic sequences. mGene is a computational tool for the genome-wide prediction of protein coding genes from eukaryotic DNA sequences.

Which of the following is an example of composite database?

  1. OWL

  2. GenBank

  3. NRL-3D

  4. PIR

  5. SWISS-PROT


Correct Option: A
Explanation:

Correct answer. Composite database: A database that amalgamates a number of primary sources, using a set of defined criteria that determine the priority of inclusion of the different sources and the level of redundancy retained. OWL is a composite database that amalgamates information from SWISS-PROT, PIR, GenBank and NRL-3D databases.

Which of the following statements are FALSE?

  1. Synteny refers to local conservation of gene order.
  2. The helix content of a protein can be determined using circular dichromism spectroscopy.
  3. In zinc finger proteins, the amino acid residues that coordinate zinc are Cys and Met.
  4. The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors.
  1. Only 1 and 2

  2. Only 1 and 3

  3. Only 2 and 3

  4. Only 2 and 4

  5. Only 3 and 4


Correct Option: B
Explanation:

Yes, this is the correct answer, as both the statements are false. Synteny refers to gene duplication from a common ancestor. This is the condition in which two or more gene loci are present on the same chromosome. During evolution, rearrangements may cause the loss of synteny, or else it will be retained. Translocations can cause gain of synteny. In zinc finger proteins, the amino acid residues that coordinate zinc are Cys and His. Zinc fingers coordinate zinc ions with a combination of cysteine and histidine residues. They can be classified by the type and order of these zinc coordinating residues (e.g., Cys2His2, Cys4, and Cys6).

Arrange the following in a sequence of signal transduction pathway.

P. Hormone Q. 7 TM receptor R. G protein S. cAMP T. PKA

  1. P → Q → R → S → T

  2. P → R → Q → S → T

  3. P → S → R → Q → T

  4. T → Q → R → S → P

  5. T → S → R → Q → P


Correct Option: A
Explanation:

It is correct. Correct sequence of signal transduction pathway is Hormone (P) → 7 TM receptor (Q) → G protein (R) → cAMP (S) → PKA (T).

Which of the photoreceptors use FAD as chromophore?

  1. Phototropins
  2. Phytochrome
  3. Cryptochrome
  4. BLUF-proteins
  1. Only 1 and 2

  2. Only 1 and 3

  3. Only 2 and 3

  4. Only 2 and 4

  5. Only 3 and 4


Correct Option: E
Explanation:

Correct answer. The cryptochrome and the BLUF-proteins use flavin adenine dinucleotide (FAD) as chromophore.

Which of the following is a pair of secondary databases?

  1. NRDB and MIPSX

  2. PRINTS and BLOCKS

  3. GenBank and DDBJ

  4. PIR and MIPS

  5. EMBL and TrEMBL


Correct Option: B
Explanation:

Correct answer. PRINTS and BLOCKS are secondary databases. Secondary database: A database that contains information derived from primary sequence data, typically in the form of regular expressions (patterns), fingerprints, blocks, profiles or Hidden Markov Models. PRINTS: Primary source- OWL; Stored Information - Aligned motifs (fingerprints) BLOCKS: Primary source- PROSITE/PRINTS; Stored Information - Aligned motifs (blocks)

Which of the following is a set of primary nucleic acid sequence databases?

  1. PIR, MIPS, SWISS-PROT

  2. TrEMBL, NRL-3D, EMBL

  3. EMBL, TrEMBL, GenBank

  4. EMBL, GenBank, DDBJ

  5. SWISS-PROT, EMBL, TrEMBL


Correct Option: D
Explanation:

Correct answer. EMBL, GenBank and DDBJ are primary nucleic acid sequence databases. Primary database is a database that stores biomolecular sequences of proteins, nucleic acids, etc. and associated annotation information of organism, species, function, etc.

Which of the following is an unsaturated fatty acid having two carbon-carbon double bonds in its structure?

  1. Lauric acid

  2. Myristic acid

  3. Palmitic acid

  4. Oleic acid

  5. Linoleic acid


Correct Option: E
Explanation:

Correct answer. Linoleic acid, CH3(CH2)4CH=CHCH2CH=CH(CH2)7COOH, is a polyunsaturated omega-6 fatty acid. It has two carbon-carbon double bonds in its structure.

Which of the following is/are example(s) of derived protein structure database(s)?

  1. CATH
  2. PDB
  3. SCOP
  1. Only 2

  2. Only 1 and 2

  3. Only 1 and 3

  4. Only 2 and 3

  5. All of these


Correct Option: C
Explanation:

It is correct, as Structural Classification of Proteins (SCOP) database and CATH Protein Structure Classification are examples of derived protein structure databases.

Match the column – I (common name of fatty acid) with column – II (its IUPAC name) and select the correct option.

 
Column – I Column – II
A. Palmitic acid 1. Octanoic acid
B. Stearic acid 2. Decanoic acid
C. Capric acid 3. Octadecanoic acid
D. Caprylic acid 4. Hexadecanoic acid
  1. A - 3, B - 4, C - 2, D - 1

  2. A - 2, B - 3, C - 4, D - 1

  3. A - 4, B - 2, C - 1, D - 3

  4. A - 4, B - 3, C - 2, D - 1

  5. A - 3, B - 2, C - 4, D - 1


Correct Option: D
Explanation:

Correct answer. Caprylic acid, CH3(CH2)6COOH, is a saturated fatty acid with an 8-carbon chain and has the IUPAC name octanoic acid. Capric acid, CH3(CH2)8COOH, is a saturated fatty acid with an 10-carbon chain and has the IUPAC name decanoic acid. Stearic acid, CH3(CH2)16COOH, is a saturated fatty acid with an 18-carbon chain and has the IUPAC name octadecanoic acid. Palmitic acid, CH3(CH2)14COOH, is a saturated fatty acid with an 16-carbon chain and has the IUPAC name hexadecanoic acid.

Match the column – I (disease) with column – II (caused by) and select the correct option.

 
Column – I
Column – II
A. Bacterial speck of tomato 1. Pseudomonas syringae
B. Powdery mildew of barley 2. Blumeria graminis
C. Citrus canker 3. Xanthomonas axonopodis
D. Angular leaf spot of cotton 4. Xanthomonas campestris
  1. A - 1, B - 2, C - 3, D - 4

  2. A - 2, B - 3, C - 4, D - 1

  3. A - 4, B - 2, C - 1, D - 3

  4. A - 3, B - 2, C - 4, D - 1

  5. A - 4, B - 3, C - 2, D - 1


Correct Option: A
Explanation:

Correct answer. Bacterial speck of tomato is caused by Pseudomonas syringae. Powdery mildew is a fungal disease of barley caused by Blumeria graminis. Citrus canker is a disease affecting Citrus species caused by the bacterium Xanthomonas axonopodis. Angular leaf spot of cotton is caused by Xanthomonas campestris.

Which of the following is an example of a program for DNA sequence assembly?

  1. ProDom

  2. Phrap

  3. PHYLIP

  4. Pfam

  5. PROSITE


Correct Option: B
Explanation:

Phrap is a widely used program for DNA sequence assembly and finishing tools from the UW Genome Centre.

Match the column – I (amino acid) with column – II (single letter code) and select the correct option.

 
Column – I
Column – II
A. Glutamine 1. K
B. Lysine 2. Q
C. Tryptophan 3. W
D. Tyrosine 4. Y
  1. A - 3, B - 4, C - 2, D - 1

  2. A - 2, B - 1, C - 3, D - 4

  3. A - 2, B - 3, C - 1, D - 4

  4. A - 3, B - 2, C - 1, D - 4

  5. A - 3, B - 1, C - 4, D - 2


Correct Option: B
Explanation:

Here, all are matched correctly. Single letter code for glutamine is Q. Single letter code for lysine is K. Single letter code for tryptophan is W. Single letter code for tyrosine is Y.

Pyrrolidine-2-carboxylic acid is IUPAC name of

  1. alanine

  2. glutamine

  3. lysine

  4. proline

  5. valine


Correct Option: D
Explanation:

Pyrrolidine-2-carboxylic acid is IUPAC name of proline.

Assertion (A): Gram-positive bacteria are generally resistant to complement-mediated lysis. Reason (R): Thick peptidoglycan layer in Gram-positive bacteria prevents insertion of membrane attack complex into the inner membrane.

Directions: In the following question there are two statements, Assertion (A) and Reason (R), consider both the statements independently and mark your answers as under:

1. If A and R both are correct and R is correct explanation of A.
2. If A and R both are correct and R is not correct explanation of A.
3. If A is correct and R is incorrect.
4. If A is incorrect and R is correct.
5. If A and R both are incorrect.

  1. 1

  2. 2

  3. 3

  4. 4

  5. 5


Correct Option: A
Explanation:

Yes, it is the correct answer. A and R both are correct and R is correct explanation of A. Gram-positive bacteria are generally resistant to complement-mediated lysis because thick peptidoglycan layer prevents insertion of membrane attack complex into the inner membrane.

Assertion (A): Dimethyl sulphoxide (DMSO) is used as a cryopreservant for mammalian cell cultures. Reason (R): It easily penetrates cells.

Directions: In the following question there are two statements, Assertion (A) and Reason (R), consider both the statements independently and mark your answers as under:

1. If A and R both are correct and R is correct explanation of A.
2. If A and R both are correct and R is not correct explanation of A.
3. If A is correct and R is incorrect.
4. If A is incorrect and R is correct.
5. If A and R both are incorrect.

  1. 1

  2. 2

  3. 3

  4. 4

  5. 5


Correct Option: C
Explanation:

This is the correct answer. A is correct and R is incorrect. Dimethyl sulphoxide (DMSO) is used as a cryopreservant for mammalian cell cultures to protect cells from ice crystal induced mechanical injury. It protects cells by preventing crystallisation of water.

Assertion (A): PROSITE is a composite protein sequence database. Reason (R): It contains information derived from SWISS-PROT in the form of regular expressions (patterns).

Directions: In the following question there are two statements, Assertion (A) and Reason (R), consider both the statements independently and mark your answers as under:

1. If A and R both are correct and R is correct explanation of A.
2. If A and R both are correct and R is not correct explanation of A.
3. If A is correct and R is incorrect.
4. If A is incorrect and R is correct.
5. If A and R both are incorrect.

  1. 1

  2. 2

  3. 3

  4. 4

  5. 5


Correct Option: D
Explanation:

Correct answer. A is incorrect and R is correct. PROSITE is a secondary protein sequence database. A secondary database contains information derived from primary sequence data, typically in the form of regular expressions (patterns), fingerprints, blocks, profiles or Hidden Markov Models. Stored information in PROSITE database is regular expressions (patterns) and primary source is SWISS-PROT.

Assertion (A): Hyposecretion of somatotropin hormone (STH) causes Simond’s disease. Reason (R): STH is secreted by posterior lobe of pitutary.

Directions: In the following question there are two statements, Assertion (A) and Reason (R), consider both the statements independently and mark your answers as under:

1. If A and R both are correct and R is correct explanation of A.
2. If A and R both are correct and R is not correct explanation of A.
3. If A is correct and R is incorrect.
4. If A is incorrect and R is correct.
5. If A and R both are incorrect.

  1. 1

  2. 2

  3. 3

  4. 4

  5. 5


Correct Option: C
Explanation:

Correct answer. A is correct and R is incorrect. Hyposecretion of somatotropin hormone (STH) causes Simond’s disease. STH is secreted by anterior lobe of pitutary.

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