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Biotechology and Its Applications (MPDMAT)

Description: proteins
Number of Questions: 15
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Tags: proteins Biotechnology and Its Applications
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Cry3Bb1 protein toxin provides resistance to plants from

  1. corn borer

  2. cotton bollworm

  3. corn rootworm

  4. corn earworm


Correct Option: C
Explanation:

Bacillus thuringiensis Cry3Bb1 protein toxin provides resistance to plants from corn rootworm.

Bt toxin is produced by B. thuringiensis in inactive form. Which of the following is responsible for the conversion of inactive Bt toxin into its active form?

  1. Acidic pH of the insect gut

  2. Alkaline pH of the insect gut

  3. Action of gut enzymes

  4. The microflora of the gut


Correct Option: B
Explanation:

The alkaline digestive tract of the insects denatures the inactive toxin crystals making them soluble and thus, amenable to being cut with proteases found in the insect gut. This liberates the active toxin from the crystal, which then destroys the insect.

Who coined the term biotechnology?

  1. Lowell J. Reed

  2. Paulien Hogeweg and Ben Hesper

  3. George E. Davis

  4. Karoly Ereky


Correct Option: D
Explanation:

The term biotechnology was coined by Hungarian engineer Karoly Ereky in 1919.

In which of the following plants was RNAi technology used to increase the stearic acid content?

  1. Canola, peanut, cotton

  2. Cotton

  3. Wheat, sweet potato, maize

  4. Peanut


Correct Option: B
Explanation:

RNAi technology was used to increase the stearic acid content of cotton. SAD1 gene was used for this purpose.

Tobacco is credited as

  1. the first crop plant genome sequenced

  2. the first transgenic plant

  3. the first edible vaccine to face human trial

  4. isolation of GFP protein


Correct Option: B
Explanation:

Tobacco is credited as the first transgenic plant to be produced.

Which of the following traits is unique to golden rice?

  1. Resistance to pests

  2. High concentration of vitamin A

  3. Herbicide resistance

  4. Hypo-allergic


Correct Option: B
Explanation:

Golden rice contains high concentration of vitamin A. This genetically modified rice is capable of producing beta-carotene in the grain, which is a precursor for vitamin A.

Which of the following has been developed to produce vaccine against hepatitis B?

  1. Banana

  2. Alfalfa

  3. Tobacco

  4. Tomato


Correct Option: A
Explanation:

Bananas have been developed that produce a human vaccine against hepatitis B.

Which transgenic plant is used to produce Taliglucerase alfa, a drug used to treat Gaucher's disease?

  1. Transgenic potato

  2. Transgenic carrot

  3. Transgenic tomato

  4. Transgenic rice


Correct Option: B
Explanation:

Transgenic carrots have been used to produce the drug Taliglucerase alfa, which is used to treat Gaucher's disease.

Which of the following are produced using cancerous cell lines?

  1. Transgenic plants

  2. Vaccines

  3. Monoclonal antibodies

  4. Bt plants


Correct Option: C
Explanation:

Monoclonal antibodies are produced using cancerous cell lines. The antibody producing cell is fused with cancerous cell lines to produce monoclonal antibodies.

Which of the following techniques is used to detect specific proteins in a sample of tissue homogenate or extract?

  1. Southern blot

  2. Western blot

  3. Northern blot

  4. Gel electrophoresis


Correct Option: B
Explanation:

The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein.

Which of the following is a disadvantage of using non-viral vector for gene therapy?

  1. Potential for tissue specificity

  2. Production

  3. Toxicity

  4. Gene delivery efficiency


Correct Option: D
Explanation:

Non-viral vectors have low gene delivery efficiency, which is a key obstacle for clinical applications.

Which of the following is a proposed regulatory body in India for uses of biotechnology products?

  1. DBT

  2. BRAI

  3. Biosafety Committee

  4. NIH


Correct Option: B
Explanation:

The Biotechnology Regulatory Authority of India (BRAI) is a proposed regulatory body in India for uses of biotechnology products including genetically modified organisms (GMOs). The institute was first suggested under the Biotechnology Regulatory Authority of India (BRAI) draft bill prepared by the Department of Biotechnology in 2008. Since then, it has undergone several revisions.

Naked DNA approach of gene therapy has so far been explored for

  1. over-expression of the required gene

  2. replacement of defective genes

  3. vaccine research

  4. HIV cure


Correct Option: C
Explanation:

Naked DNA approach of gene therapy has been explored for vaccine research. Naked DNA is histone-free DNA that is passed from cell to cell during a gene transfer process called transformation or transfection.

Transgene α-interferon was used for hepatitis treatment. Which of the following was/were used as donor(s) of the gene?

  1. Catfish

  2. Cattle

  3. Humans

  4. Chicken


Correct Option: C
Explanation:

α-interferon gene was taken from human cells.

The milk of which transgenic organisms is used to produce biosteel?

  1. Primates

  2. Cats

  3. Cattle

  4. Goats


Correct Option: D
Explanation:

Biosteel is a trademark name for a high-strength based fibre material made of the recombinant spider silk-like protein extracted from the milk of transgenic goats.

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