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Life Sciences

Description: GATE
Number of Questions: 15
Created by:
Tags: GATE Recombinant DNA Technology Bacteriophage and other Viral Vectors Sequence and Structure Databases
Attempted 0/15 Correct 0 Score 0

The part of an antibody that recognizes the anti-genic determinant is called _________.

  1. hapten

  2. paratope

  3. immunogen

  4. allergen

  5. mimotope


Correct Option: B
Explanation:

The part of an antibody that recognizes the anti-genic determinant is called a paratope. Thus, this option is correct.

Which among the following are primary lymphoid organs?

  1. Thymus
  2. Bone marrow
  3. Spleen
  4. Lymph nodes
  5. Mucosa-associated lymphoid tissue
  1. 1 and 2

  2. 1, 2 and 4

  3. 2 and 3

  4. 2, 3 and 5

  5. 4 and 5


Correct Option: A
Explanation:

The thymus and the bone marrow constitute the primary lymphoid tissues involved in the production and early selection of lymphocyte tissues. Thymus: Site of T Cell maturation Bone Marrow: Site of Haematopoiesis and B Cell maturation

Which of the following proteins enhances follicle-stimulating hormone (FSH) biosynthesis and secretion?

  1. Bindin

  2. Avidin

  3. Activin

  4. Inhibin

  5. Hyalin


Correct Option: C
Explanation:

Activin is a protein complex which enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle.

Assertion: In capping, methyl guanosine triphosphate is added to the 3'-end of hnRNA. Reason: In tailing, adenylate residues are added at 5'-end in a template independent manner.

Direction: In the following question there are two statements, Assertion (A) and Reason (R), consider both the statements independently and mark your answers as under:

1. A and R both are correct and R is correct explanation of A.
2. A and R both are correct and R is not correct explanation of A.
3. A is correct and R is incorrect.
4. A is incorrect and R is correct.
5. A and R both are incorrect.

  1. A and R are both correct and R is the correct explanation of A.

  2. A and R are both correct and R is not the correct explanation of A.

  3. A is correct and R is incorrect.

  4. A is incorrect and R is correct.

  5. A and R are both incorrect.


Correct Option: E
Explanation:

Both are incorrect statements. In capping, methyl guanosine triphosphate is added to the 5'-end of hnRNA. In tailing, adenylate residues are added at 3'-end in a template independent manner. It is the fully processed hnRNA, now called mRNA, that is transported out of the nucleus for translation.

Which of the following statements is/are not correct about deoxyribonucleic acid?

  1. Histones are rich in the acidic amino acid moities.
  2. A typical nucleosome contains 200 bp of DNA helix.
  3. The chromatin that is more densely packed and stains dark are called as euchromatin.
  4. Euchromatin is a transcriptionally active chromatin.
  1. 1 and 2

  2. 1 and 3

  3. 1 and 4

  4. 2 and 3

  5. 3 and 4


Correct Option: B
Explanation:

In eukaryotes, there is a set of positively charged, basic proteins called histones. Histones are rich in the basic amino acid residues lysines and arginines. In a typical nucleus, some region of chromatin are loosely packed (and stains light) and are referred to as euchromatin. The chromatin that is more densely packed and stains dark are called as Heterochromatin.

Which of the following terms refers to PCD?

  1. Apoptosis
  2. Necrosis
  3. Entosis
  4. Anoikis
  1. 1 and 2

  2. 1 and 4

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: B
Explanation:

Apoptosis is the process of programmed cell death that may occur in multicellular organisms where biochemical events lead to characteristic changes in morphology of cell and death. These changes include blebbing, cell shrinkage, nuclear fragmentation, chromatin condensation and chromosomal DNA fragmentation. Anoikis is a form of programmed cell death which is induced by anchorage-dependent cells detaching from the surrounding extracellular matrix.

Which of the following bacteriophages was the first DNA-based genome to be sequenced?

  1. Φ X 174 phage

  2. P1 phage

  3. λ phage

  4. T4 phage

  5. MS2 phage


Correct Option: A
Explanation:

The phi X 174 bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.

Which of the following statements are not true?

  1. Pseudogenes are inactive copies of protein-coding genes.
  2. About 80% of the human genome consists of repetitive DNA sequences.
  3. The human reference genome is a diploid sequence.
  4. The total length of the human genome is over 3 billion base pairs.
  1. 1 and 2

  2. 1 and 3

  3. 1 and 4

  4. 2 and 3

  5. 2 and 4


Correct Option: D
Explanation:

About 8% of the human genome consists of repetitive DNA sequences, termed as tandem repeats or tandem DNA arrays. The human reference genome (HRG) is a haploid sequence. Each chromosome is represented once.

Which of the following sets represents filamentous bacteriophages?

  1. M13 phage, T12 phage, fd phage

  2. M13 phage, f1 phage, fd phage

  3. T2 phage, f1 phage, fd phage

  4. M13 phage, MS2 phage, f1 phage

  5. MS2 phage, T12 phage, f1 phage


Correct Option: B
Explanation:

M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA). Filamentous bacteriophage fd is a type of filamentous bacteriophage known to infect Escherichia coli. Bacteriophage f1 is structurally classified as a class I filamentous phage.

Match the column – I with column – II and select the correct option ||| |---|---| |Column – I |Column – II| |A. Heterogeneous nuclear RNA|1. Pol I| |B. Ribosomal RNA|2. RNAP II and Pol II| |C. Small nuclear RNA|3. Pol III|

  1. A - 1, B - 2, C - 3

  2. A - 2, B - 1, C - 3

  3. A - 2, B - 3, C - 1

  4. A - 3, B - 2, C - 1

  5. A - 3, B - 1, C - 2


Correct Option: B
Explanation:

In eukaryotes, there are at least three RNA polymerases in the nucleus (in addition to the RNA polymerase found in the organelles). There is a clear cut division of labour. The RNA polymerase I or Pol I transcribes ribosomal RNAs (28S, 18S, and 5.8S), whereas the RNA polymerase III or Pol III is responsible for transcription of tRNA, 5srRNA, and snRNAs (small nuclear RNAs). The RNA polymerase II or RNAP II and Pol II transcribes precursor of mRNA, the heterogeneous nuclear RNA (hnRNA).

Which of the following statements is/are correct about the structure of deoxyribonucleic acid?

  1. It is made of two polynucleotide chains, where the backbone is constituted by sugar-phosphate, and the bases project outside.
  2. The two chains have anti-parallel polarity.
  3. Adenine forms three hydrogen bonds with thymine from opposite strand.
  4. The two chains are coiled in a right-handed fashion.
  1. 1 and 2

  2. 1 and 4

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: D
Explanation:

The two chains have anti-parallel polarity. It means, if one chain has the polarity 5'→3', the other has 3'→5'. The two chains are coiled in a right-handed fashion. The pitch of the helix is 3.4 nm and there are roughly 10 bp in each turn. Consequently, the distance between a bp in a helix is approximately equal to 0.34 nm.

Which of the following is a pair of bibliographic databases?

  1. EMBL and PDB

  2. PDB and Sequin

  3. Embase and EMBL

  4. BioOne and PDB

  5. BioOne and Embase


Correct Option: E
Explanation:

BioOne bibliographic database is an indexed and fully-searchable collection of abstracts that link to the fulltext articles available from the BioOne organization. Embase is a biomedical and pharmacological database containing bibliographic records with citations, abstracts and indexing derived from biomedical articles in peer reviewed journals and is especially strong in its coverage of drug and pharmaceutical research.

The International Nucleotide Sequence Database (INSD) consists of which of the following databases?

  1. DNA Data Bank of Japan
  2. European Nucleotide Archive
  3. GenBank
  4. PROSITE
  5. VectorBase
  1. 1, 2 and 3

  2. 1, 3 and 5

  3. 2, 3 and 4

  4. 2, 4 and 5

  5. 3, 4 and 5


Correct Option: A
Explanation:

The International Nucleotide Sequence Database Collaboration (INSDC) is a joint effort to collect and disseminate databases containing DNA and RNA sequences. It consists of the following databases: DNA Data Bank of Japan (Japan), GenBank (USA) and the European Nucleotide Archive (UK).

Which of the following is an incorrect statement?

  1. PCR is a laboratory technique for amplifying a specific DNA sequence.

  2. Gel electrophoresis is a basic technique used to separate DNA, RNA or proteins.

  3. The northern blotting is a laboratory method used to detect specific RNA molecules from a mixture of RNA molecules.

  4. Electroporation used to transfer polar molecules like DNA into host cells.

  5. The southern blotting technique depends on similarities between the sequences of probe RNA and experimental RNA.


Correct Option: E
Explanation:

The southern blotting technique is a laboratory method used to detect specific DNA molecules from many other DNA molecules. The southern blotting technique depends on similarities between the sequences of probe DNA and experimental DNA.

Match the column – I with column – II and select the correct option ||| |---|---| |Column – I |Column – II| |A. DDBJ|1. Bibliographic database| |B. MEDLINE|2. Nucleotide sequence database| |C. NatureServe|3. Model organism database| |D. WormBase|4. Biodiversity database|

  1. A - 4, B - 3, C - 2, D - 1

  2. A - 2, B - 1, C - 4, D - 3

  3. A - 2, B - 1, C - 3, D - 4

  4. A - 3, B - 2, C - 4, D - 1

  5. A - 1, B - 2, C - 3, D - 4


Correct Option: C
Explanation:

DNA Data Bank of Japan (DDBJ) is a nucleotide sequence database. It is a biological database that collects DNA sequences. MEDLINE is a bibliographic database of life sciences and biomedical information which provides bibliographic information for articles from academic journals covering medicine, nursing, pharmacy, dentistry, veterinary medicine and health care. NatureServe is a sophisticated biodiversity database which acts as an authoritative source for information on more thant 70,000 plants, animals, and ecosystems of the United States and Canada. WormBase is an online biological database about the biology and genome of the model organism Caenorhabditis elegans (a nematode) and contains information about other related nematodes.

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