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Biochemistry

Description: GATE: Life Science : Biochemistry
Number of Questions: 20
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Tags: biochemistry Biochemistry
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Which of the following is a component of myosin?

  1. Creatine

  2. 4-hydroxyproline

  3. γ-carboxyglutamate

  4. Desmosine

  5. 6-N methyl lysine


Correct Option: E
Explanation:

6-N methyl lysine is a component of myosin, which is the contractile protein found in muscles. 6-N methyl lysine is an amino acid which occurs naturally in biofluids of human beings.

Which of the following statements is correct about Vitamin-D3 or Cholecalciferol?

  1. It is produced non-enzymetically in skin from 7-dehydrocholesterol.

  2. It is an antioxidant.

  3. It is composed of imidazole and thiophene ring fused together.

  4. It contains pterin ring connected with p-aminobenzoic acid.

  5. It is a corrinoid bearing the corrin ring.


Correct Option: A
Explanation:

Vitamin-D3 or Cholecalciferol is synthesized in skin. At first previtamin-D is produced non-enzymetically in skin from 7-dehydrocholesterol with the help of UV rays of sunlight. Then it undergoes several reactions for some time to form cholecalciferol.

Which of the following is the odd one?

  1. Thromboxanes

  2. Prostaglandins

  3. Leukotrienes

  4. Cholesterol

  5. Both 2 and 4


Correct Option: D
Explanation:

Cholesterol is an important sterol found in animal tissues. A sterol is the one in which the steroid nucleus (4 fused rings in which 3 rings have 6 carbons and 1 has 5 carbons) contains one or many hydroxyl groups but no carboxyl or carbonyl groups. It is an important component of plasma lipoproteins and plasma membrane. Cholesterol has a polar hydroxyl group on C-3 and also a hydrocarbon side chain on C-17.

Which of the following is the correct statement about hyaluronic acid?

  1. Short polymer of D-glucoronic acid and N-acetylgalactosamine.

  2. A polymer of D-galactose and N-acetylglucosamine.

  3. A polymer of N-acetylglucosamine and D-glucoronic acid.

  4. A polymer of D-glucosamine-N-sulphate and D-glucoronic acid.

  5. None of these


Correct Option: C
Explanation:

Hyaluronic acid is the straight chain polymer of N-acetylglucosamine and D-glucoronic acid,  which is linked together by (β-1 -> 3) linkages. Hyaluronic acid or hyaluronate is a glycosaminoglycan,  which is found in abundance in animal tissues and also in bacteria.

Which of the following are contained in Gangliosides?

  1. Galactose, glucose and ceramide

  2. N-acetyl-D-galactosamine and ceramide

  3. Sialic acid

  4. Glucose and galactose

  5. Both 1 and 3


Correct Option: E
Explanation:

Gangliosides are glycosphingolipids which are obtained from glucosylceramide. Gangliosides can contain more than one molecule of sialic acid along with galactose, glucose and ceramide. They are present in the nervous tissues in the form of membrane lipids.

Which of the following disaccharides is formed by the hydrolysis of starch?

  1. Sucrose

  2. Maltose

  3. Trehalose

  4. Lactose

  5. None of the above


Correct Option: B
Explanation:

Maltose (C12H22O11) is a reducing disaccharide which is obtained from the hydrolysis of starch with the help of amylases. Maltose in α and β forms are linked with α-1, 4-glycosidic linkage. Maltose is a crystalline solid, white in color and has melting point of about 160-165°C.

What does an α-helix signify?

  1. Aggregation of protein.

  2. Tertiary structure of protein.

  3. Secondary structure of protein.

  4. Primary structure of protein.

  5. None of the above


Correct Option: C
Explanation:

The secondary structure of protein is formed by the folding of linear polypeptide chain into regular structure. The folding of polypeptide chain occurs because NH and CO group in the peptide chain are linked with hydrogen bonds. The α-helix is a rod-like structure. There is an imaginary axis at the middle of the helix. Around the imaginary axis, the polypeptide chain is wound tightly.

Which of the following is a simple protein?

  1. Keratin

  2. Chymotripsinogen

  3. Glycoproteins

  4. Lipoproteins

  5. Myoglobulin


Correct Option: B
Explanation:

Depending on the composition, simple proteins are those proteins, which consist of amino acid residues only. They do not contain any other chemical components. Chymotripsinogen is one such simple protein.

Which Vitamin-A derivative is obtained from oxidation of retinal?

The various forms of Vitamin-A work as hormone and are present in the vertebrate eye in the form of visual pigment. Vitamin-A activity is seen in both carotenoids and retinoids.

  1. β carotene

  2. Retinoic acid

  3. Retinol

  4. Retin-A

  5. None of these


Correct Option: B
Explanation:

Retinoic acid, a Vitamin-A derivative is obtained from oxidation of retinal. 9-cis retinoic acid and trans-retinoic acid control development, growth and differentiation of tissues. It also controls gene expression.

Which vitamin acts as the coenzyme in decarboxylation and transamination of amino acids?

  1. Niacin

  2. Thiamin

  3. Biotin

  4. Ascorbic acid

  5. Pyridoxine


Correct Option: E
Explanation:

Pyridoxine or Vitamin-B6 plays a major role in the metabolism of amino acids and glycogen. It acts as the coenzyme in decarboxylation and transamination of amino acids. It also plays a significant role in the action of steroid hormone.

Depending on polarity and charge of R groups match the following amino acids: Name Type

  1. Glycine A. Polar Uncharged amino acid
  2. Glutamine B. Non-polar aliphatic amino acid
  3. Tyrosine C. Negatively charged amino acid
  4. Glutamate D. Positively charged amino acid
  5. Histidine E. Aromatic amino acid
  1. 1 - B, 2 - D, 3 - E, 4 - C, 5 - A

  2. 1 - B, 2 - A, 3 - E, 4 - C, 5 - D

  3. 1 - C, 2 - A, 3 - E, 4 - B, 5 - D

  4. 1 - B, 2 - D, 3 - E, 4 - A, 5 - C

  5. 1 - B, 2 - E, 3 - D, 4 - C, 5 - A


Correct Option: B
Explanation:

Non-polar aliphatic amino acids have hydrophobic and non-polar R groups. Glycine is a non-polar aliphatic amino acid. Polar uncharged amino acids have hydrophilic R groups. Glutamine is a polar uncharged amino acid. Aromatic amino acids are non-polar and contain aromatic side chains. Tyrosine is an aromatic amino acid. Negatively charged amino acids possess R groups with net negative charge. Glutamate is a negatively charged amino acid. Positively charged amino acids have R groups, which are hydrophilic and with positive charge. Histidine is a positively charged amino acid.

Deficiency of Vitamin-A causes which of the following?

The various forms of Vitamin-A work as hormone and are present in the vertebrate eye in the form of visual pigment. Vitamin-A activity is seen in both carotenoids and retinoids.

  1. Pellagra

  2. Affects liver and central nervous system

  3. Dermatitis

  4. Xerophthalmia and dryness of eyes, mucous membrane and skin

  5. Haemorrhagic disease


Correct Option: D
Explanation:

Xerophthalmia is a disease in which the cornea and conjunctiva of the eye get dried due to prolonged Vitamin-A deficiency. In this condition the eye fails to produce tears. Vitamin-A deficiency also causes dryness in mucous membrane, skin and delayed growth and development.

Which of the following is the odd one?

Fatty acids are found in oils, natural fats and complex lipids in the form of esters. Fatty acids are hydrocarbons with long chain which have 4-36 C atoms in addition to one carboxylic acid.

  1. Oleic acid

  2. Linoleic acid

  3. Arachidonic acid

  4. Timnodonic acid

  5. Linolenic acid


Correct Option: A
Explanation:

Oleic acid belongs to the class of monounsaturated fatty acids also known as monoenoic acids. Oleic acid consists of one double bond. Its systematic name is cis-9-Octadecenoic acid. Oleic acid is commonly found in natural fats.

Which glycoprotein has O-glycosidic linkage?

Glycoconjugate molecules are biologically active. They are formed as a result of the covalent bonding of oligosaccharides and polysaccharides with lipid or protein molecules.

  1. Plasma protein

  2. Mucin

  3. GPI-linked glycoprotein

  4. Uromodulin

  5. All of the above


Correct Option: B
Explanation:

Mucin contains O-glycosidic bond between the hydroxyl side-chain of Thr or Ser and the anomeric carbon. Mucins are capable of forming gels and therefore is an important ingredient of gel-like secretions.

Which glycoconjugate can have more than one oligosaccharide chain along with covalent linkage with the polypeptide backbone?

Glycoconjugate molecules are biologically active. They are formed as a result of the covalent bonding of oligosaccharides and polysaccharides with lipid or protein molecules.

  1. Proteoglycan

  2. Peptidoglycan

  3. Glycoprotein

  4. Ganglioside

  5. None of these


Correct Option: C
Explanation:

The proteins having one or more oligosaccharide chains bound covalently to side-chains of polypeptides are called glycoproteins. Glycoproteins like TSH, HCG, FSH, etc., act as hormones. Other glycoproteins include transferrin, immunoglobin, lectin, calnexin, etc.

Which of the following is the polysaccharide, which produces cellobiose and β-D-glucose on hydrolysis?

This fibrous structural polysaccharide is present in abundance in the plant cell walls but absent in animals.

  1. Peptidoglycan

  2. Hyaluronic acid

  3. Starch

  4. Inulin

  5. Cellulose


Correct Option: E
Explanation:

Cellulose on hydrolysis produces cellobiose and β-D-glucose. The first step involves breaking of β-glycosidic bonds with the help of β-1, 4 glucanase to form cellobiose. Then, the β-1, 4 glycosidic bond is cleaved with the help of β-glucosidase to form glucose.

Match the vitamins with their deficiency diseases

  1. Vitamin B2 A. Low and scurvy healing of wounds
  2. Vitamin A B. Pernicious anaemia
  3. Vitamin C C. abrasion at corners of lips, tongue and mouth
  4. Vitamin D D. night blindness
  5. Vitamin B12 E. Rickets
  1. 1 - C, 2 - D, 3 - A, 4 - E, 5 - B

  2. 1 - B, 2 - D, 3 - A, 4 - E, 5 - C

  3. 1 - C, 2 - A, 3 - D, 4 - E, 5 - B

  4. 1 - D, 2 - C, 3 - A, 4 - E, 5 - B

  5. 1 - C, 2 - D, 3 - E, 4 - A, 5 - B


Correct Option: A
Explanation:

Vitamin B2 acts as the coenzyme in reactions where oxidation and reduction take place. Its deficiency causes abrasion at corners of lips, tongue and mouth. Vitamin A occurs in the retina of vertebrates in the form of visual pigments. Its deficiency causes night blindness. Vitamin C is an antioxidant and its deficiency causes low and scurvy healing of wounds. Vitamin D maintains balance of calcium and its deficiency causes rickets due to improper mineralization of bones. Vitamin B12 acts as the coenzyme in metabolism of folic acid and its deficiency causes pernicious anaemia.

Match the following

  1. Starch A. It is stored in the liver of animals
  2. Dextrans B. It is found in roots and tubers of onions, garlic, dahlias and dandelions
  3. Glycogen C. It is found in amyloplasts and chloroplasts ofplant cells
  4. Chitin D. It is found in yeast and bacteria
  5. Inulin E. It occurs in insects and arthropods like crabsand also in fungi.
  1. 1 - C, 2 - E, 3 - A, 4 - B, 5 - D

  2. 1 - E, 2 - D, 3 - A, 4 - B, 5 - C

  3. 1 - B, 2 - D, 3 - E, 4 - C, 5 - A

  4. 1 - C, 2 - D, 3 - A, 4 - E, 5 - B

  5. 1 - D, 2 - C, 3 - A, 4 - E, 5 - B


Correct Option: D
Explanation:

Starch is found in amyloplasts and chloroplast of plant cells in higher plants in the form of compact and insoluble granules. Dextrans occur in yeast and bacteria in the form of a stock polysaccharide. Glycogen is found in abundance in the liver of animals and forms the most essential food reserve in animals. It is also called animal starch. Chitin is found in abundance in insects and arthropods like crabs. Vertebrates cannot digest chitin. Inulin occurs in roots and tubers of onions, garlic, dahlias and dandelion. It is white powdery in nature and can be used to determine the functioning of kidney by measuring Glomerular Filtration Rate.

The polysaccharide mentioned in the passage in question 19 exhibits which of the following linkages?

This fibrous structural polysaccharide is present in abundance in the plant cell walls but absent in animals.

  1. (β-1 -> 4) glycosidic linkage

  2. (β-1 -> 3) linkages

  3. (α-1 -> 6) glycosidic linkage

  4. (β-2 -> 1) fructosidic linkages

  5. None of these


Correct Option: A
Explanation:

Cellulose (a fibrous structural polysaccharide) is an unbranched and linear polymer of β-D-glucose attached with a (β-1 -> 4) glycosidic linkage. It is also known as anhydride of β-D-glucose.

Which of the following is/are called Essential Fatty Acids?

Fatty acids are found in oils, natural fats and complex lipids in the form of esters. Fatty acids are hydrocarbons with long chain which have 4-36 C atoms in addition to one carboxylic acid.

  1. Palmitoleic acids

  2. Timnodonic acids and Arachidonic acids

  3. Linolenic acids and Elaidic acids

  4. Linoleic acids, Linolenic acids and Arachidonic acids

  5. None of the above


Correct Option: D
Explanation:

Linoleic acid, arachidonic acid and linolenic acid are together known as Essential Fatty Acids (EFA). These fatty acids are not synthesized in the body and ingested by humans and animals for proper working of the cells. Two fatty acids: Linoleic acid (belonging to ω-6 fatty acid) and α-Linolenic acid (belonging to ω-3 fatty acid) are necessary in humans.

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