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Microbes (GATE)

Description: GAte
Number of Questions: 15
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Tags: GAte Role of Microorganisms in Transformation of Organic Matter and in the Causation of Diseases.
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Which of the following staining methods use(s) carbol fuchsin and methylene blue as primary stain and counter stain?

P. Gram stain Q. Kinyoun stain R. Ziehl–Neelsen stain

  1. Only P

  2. Only Q

  3. Only R

  4. P and Q

  5. Q and R


Correct Option: E
Explanation:

The reagents used in Ziehl–Neelsen staining method are carbol fuchsin (primary stain), acid alcohol and methylene blue (counter stain). The Kinyoun staining method uses carbol fuchsin as a primary stain, followed by decolourization with an acid-alcohol solution and methylene blue as a counter stain.

Which among the following are catalase-positive bacteria?

  1. Enterobacter
  2. Enterococcus
  3. Staphylococcus
  4. Streptococcus
  1. 1 and 2

  2. 1 and 3

  3. 2 and 3

  4. 2 and 4

  5. 3 and 4


Correct Option: B
Explanation:

Enterobacter and Staphylococcus are examples of catalase-positive bacteria.

Gram staining is a common technique used to differentiate Gram-positive and Gram-negative bacteria based on their different cell wall constituents. Colours of Gram-positive and Gram-negative bacteria on a Gram staining are __________ and _________, respectively.

  1. pink, purple

  2. purple, pink

  3. purple, colourless

  4. colourless, purple

  5. colourless, pink


Correct Option: B
Explanation:

The Gram stain technique distinguishes between Gram-positive and Gram-negative bacteria by colouring these cells purple or pink. Gram-positive bacteria stain purple due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet of these cells during the decolouring process. Gram-negative bacteria stain pink, which is attributed to a thinner peptidoglycan wall, which does not retain the crystal violet during the decolouring process.

Which of the following is a genus of non-sulphur photoautotrophic bacteria?

  1. Chlorobium

  2. Chromatium

  3. Rhodospirillum

  4. Beggiatoa

  5. Thiobacillus


Correct Option: C
Explanation:

Rhodospirillum is a genus of purple non-sulphur photoautotrophic bacteria of the family Rhodospirillaceae.

Transduction method of genetic recombination in Salmonella typhimurium was first demonstrated by

  1. Zinder and Lederberg

  2. Griffith

  3. Lederberg and Tautum

  4. A. V. Leeuwenhoek

  5. R. Koch


Correct Option: A
Explanation:

Transduction method of genetic recombination in bacteria, e.g. Salmonella typhimurium was first demonstrated by Zinder and Lederberg in 1952. It is the transfer of genes (small double stranded piece of DNA) from one bacterial cell to another via virus (bacteriophage).

Which of the following bacteria are called gliding bacteria?

  1. Mycobacteria and Myxobacteria

  2. Mycobacteria and Cytophagales

  3. Myxobacteria and Cytophagales

  4. Cytophagales and Rickettsiae

  5. Rickettsiae and Chlamydiae


Correct Option: C
Explanation:

Myxobacteria and Cytophagales are called gliding bacteria on the basis of their gliding mobility on solid surfaces.

Which of the following are used as counter stains in Gram staining technique?

P. Crystal violet Q. Safranin R. Iodine solution S. Carbol fuchsin

  1. P and Q

  2. P and R

  3. Q and R

  4. Q and S

  5. R and S


Correct Option: D
Explanation:

Safranin is used as secondary stain (counter stain) in Gram staining technique and carbol fuchsin is sometimes substituted for safranin as counter stain since it stains anaerobic bacteria more intensely.

Amphitrichous flagella are a characteristic feature of the genus

  1. Lactobacillus

  2. Pseudomonas

  3. Spirillum

  4. Nitrosomonas

  5. Clostridium


Correct Option: D
Explanation:

Amphitrichous: Flagella at both sides or ends, e.g. Nitrosomonas

Match column – I (Bacteria) with column – II (Flagellar arrangement) and select the correct option.

Column – I
Column – II
A. Escherichia coli 1. Amphitrichous
B. Helicobacter pylori 2. Lophotrichous
C. Rhodospirillum rubrum 3. Monotrichous
D. Vibrio cholerae 4. Peritrichous
  1. A - 4, B - 3, C - 2, D - 1

  2. A - 2, B - 1, C - 4, D - 3

  3. A - 4, B - 2, C - 1, D - 3

  4. A - 3, B - 2, C - 4, D - 1

  5. A - 1, B - 2, C - 3, D - 4


Correct Option: C
Explanation:

Peritrichous bacteria have flagella projecting in all directions (e.g. Escherichia coli). Lophotrichous bacteria have multiple flagella located at the same spot on the bacteria's surfaces (e.g. Helicobacter pylori). Amphitrichous bacteria have a single flagellum on each of two opposite ends (e.g. Rhodospirillum rubrum). Monotrichous bacteria have a single flagellum (e.g. Vibrio cholerae).

Which of the following is an example of obligate aerobes?

  1. Nocardia

  2. Veillonella

  3. Peptostreptococcus

  4. Treponema

  5. Staphylococcus


Correct Option: A
Explanation:

Nocardia is an obligate aerobe.

Which of the following stains is used to identify acid-fast bacteria, mainly Mycobacterium?

  1. Auramine-rhodamine stain

  2. Gram stain

  3. Hoechst stain

  4. Kinyoun stain

  5. Ziehl-Neelsen stain


Correct Option: E
Explanation:

Ziehl-Neelsen stain is used to identify the acid-fast bacteria, mainly Mycobacterium.

Which of the following is a genus of chemosynthetic filamentous sulphur-oxidising bacteria?

  1. Chlorobium

  2. Chromatium

  3. Rhodospirillum

  4. Beggiatoa

  5. Thiobacillus


Correct Option: D
Explanation:

Beggiatoa is a genus of chemosynthetic filamentous sulphur-oxidizing bacteria.

Which of the following pairs of microbes represents non-spore forming rods?

  1. Clostridium, Lactobacillus

  2. Bacillus, Clostridium

  3. Desulfotomaculaum, Sporosarcina

  4. Lactobacillus, Listeria

  5. Bacillus, Listeria


Correct Option: D
Explanation:

Lactobacillus and Listeria are non-spore forming rods.

Which of the following structures are not found in bacterial cells?

  1. Cell wall
  2. Mitochondria
  3. Nuclear membrane
  4. Mesosome
  5. DNA
  1. 1 and 2

  2. 2, 3 and 4

  3. 3 and 4

  4. 3, 4 and 5

  5. 4 and 5


Correct Option: C
Explanation:

Membrane bound organelles like endoplasmic reticulum, mitochondria and golgi bodies are absent in bacteria. Bacterial nucleus is of primitive type (prokaryotic), i.e. without nuclear membrane and nucleolus.

Match column – I (Disease) with column – II (Causative agent) and select the correct option.

Column – I Column – II
P. Leaf blight of rice 1. Pseudomonas fuscovaginae
Q. Angular leaf spot of cotton 2. Pseudomonas syringae
R. Halo blight of bean 3. Xanthomonas oryzae
S. Bacterial sheath rot of wheat 4. Xanthomonas campestris
  1. P - 1, Q - 3, R - 4, S - 2

  2. P - 1, Q - 3, R - 2, S - 4

  3. P - 3, Q - 2, R - 4, S - 1

  4. P - 3, Q - 4, R - 2, S - 1

  5. P - 4, Q - 2, R - 1, S - 3


Correct Option: D
Explanation:

Leaf blight of rice is caused by Xanthomonas oryzae. Angular leaf spot of cotton is caused by Xanthomonas campestris. Halo blight of bean is a bacterial disease caused by Pseudomonas syringae. Bacterial sheath rot of wheat is caused by Pseudomonas fuscovaginae.

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